##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431363_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3062293
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.17348241987295	31.0	31.0	34.0	25.0	34.0
2	30.279133969218492	33.0	31.0	34.0	25.0	34.0
3	30.408266289345924	34.0	31.0	34.0	25.0	34.0
4	33.4466963154734	37.0	35.0	37.0	26.0	37.0
5	33.76737203135036	37.0	35.0	37.0	28.0	37.0
6	33.7059383279131	37.0	35.0	37.0	28.0	37.0
7	33.67239483615709	37.0	35.0	37.0	28.0	37.0
8	33.587722010924494	37.0	35.0	37.0	27.0	37.0
9	34.905456466771795	39.0	37.0	39.0	25.0	39.0
10-11	34.975478179259795	39.0	37.0	39.0	27.0	39.0
12-13	34.89713541454067	39.0	37.0	39.0	26.5	39.0
14-15	36.14211670797014	40.0	37.0	41.0	26.5	41.0
16-17	36.156023117317645	40.0	37.0	41.0	26.5	41.0
18-19	36.18418306151632	40.0	37.0	41.0	27.0	41.0
20-21	36.24314149560476	40.0	37.0	41.0	26.5	41.0
22-23	36.29665156142799	40.0	37.0	41.0	27.0	41.0
24-25	36.35787953667399	40.0	37.0	41.0	27.0	41.0
26-27	36.38653861665098	40.0	37.0	41.0	27.5	41.0
28-29	36.36790062218083	40.0	37.0	41.0	28.0	41.0
30-31	36.291854012663066	40.0	36.0	41.0	28.0	41.0
32-33	36.188983222702724	40.0	36.0	41.0	27.5	41.0
34-35	36.09560776842713	40.0	36.0	41.0	27.0	41.0
36-37	35.972644191787005	40.0	36.0	41.0	27.0	41.0
38-39	35.839274197472285	40.0	35.5	41.0	26.5	41.0
40-41	35.70974429945142	40.0	35.0	41.0	26.0	41.0
42-43	35.54359739580765	40.0	35.0	41.0	26.0	41.0
44-45	35.388753950062906	40.0	35.0	41.0	25.5	41.0
46-47	35.226780389727566	40.0	35.0	41.0	25.0	41.0
48-49	35.06268391039002	39.0	35.0	41.0	25.0	41.0
50-51	34.88855981449195	39.0	34.5	41.0	25.0	41.0
52-53	34.6575861617422	39.0	34.0	41.0	24.5	41.0
54-55	34.43954677099808	38.5	34.0	40.5	24.0	41.0
56-57	34.18754361519293	38.0	33.5	40.0	23.5	41.0
58-59	33.8983642322926	38.0	33.0	40.0	23.0	41.0
60-61	33.67629616107929	37.0	33.0	40.0	22.0	41.0
62-63	33.46789987110965	37.0	33.0	40.0	22.0	41.0
64-65	33.177503263077696	36.5	33.0	40.0	20.5	41.0
66-67	32.84950101117039	36.0	32.0	39.5	20.0	41.0
68-69	32.524102200540575	35.0	32.0	39.0	18.5	41.0
70-71	32.27836052918515	35.0	32.0	39.0	18.0	41.0
72-73	32.035713434344785	35.0	32.0	38.0	16.0	40.0
74-75	31.70634178375485	35.0	31.5	37.0	12.0	40.0
76-77	31.3950805817732	35.0	31.0	37.0	9.0	39.0
78-79	31.08092187782162	35.0	31.0	36.0	7.0	39.0
80-81	30.78392661969315	35.0	31.0	36.0	3.5	38.5
82-83	30.503686453255778	35.0	31.0	35.5	2.0	37.0
84-85	30.24805970558663	35.0	31.0	35.0	2.0	37.0
86-87	30.01824988660458	35.0	30.0	35.0	2.0	36.5
88-89	29.798613326680368	35.0	30.0	35.0	2.0	36.0
90-91	29.600778240357798	34.0	30.0	35.0	2.0	36.0
92-93	29.421478937515126	34.0	30.0	35.0	2.0	36.0
94-95	29.203604619153033	34.0	30.0	35.0	2.0	35.0
96-97	29.022281506047918	34.0	29.5	35.0	2.0	35.0
98-99	28.900334651191116	34.0	29.5	35.0	2.0	35.0
100	28.850766729375668	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6307744847036965
1101	2	0.633657840633159
1101	3	0.5499007426790286
1101	4	0.5352151812512886
1101	5	0.6129199004532211
1101	6	0.5349236771199983
1101	7	0.5962076536256831
1101	8	0.6088553353634509
1101	9	0.7380182026548283
1101	10-11	0.7116578688393602
1101	12-13	0.6643276124848185
1101	14-15	0.7285303010442661
1101	16-17	0.6954339503843343
1101	18-19	0.6821604686919969
1101	20-21	0.6640476616058848
1101	22-23	0.6814399503369302
1101	24-25	0.7396864824365394
1101	26-27	0.8138557761399738
1101	28-29	0.8275961001427063
1101	30-31	0.9266978309493297
1101	32-33	0.9237027981390327
1101	34-35	0.9905431797178466
1101	36-37	0.9974494865331565
1101	38-39	0.9007095210351892
1101	40-41	0.9270688541836023
1101	42-43	0.8780753552728697
1101	44-45	0.9166171394891691
1101	46-47	0.9520096466482428
1101	48-49	0.941607651276847
1101	50-51	1.0072788347886075
1101	52-53	0.991394779479478
1101	54-55	0.9768374663581483
1101	56-57	0.9314204615238992
1101	58-59	0.9946331693063826
1101	60-61	1.0234608820257023
1101	62-63	0.9353116452086496
1101	64-65	0.9912485874393582
1101	66-67	0.9300401772252727
1101	68-69	0.946520695545594
1101	70-71	0.8710310268172421
1101	72-73	0.9375638763235017
1101	74-75	0.9834538894250642
1101	76-77	0.997606925900115
1101	78-79	0.9318389523649842
1101	80-81	0.9102648180677058
1101	82-83	0.9512146996293929
1101	84-85	0.9685428708814285
1101	86-87	0.9838835224620865
1101	88-89	0.9779901312788404
1101	90-91	1.0451081318614115
1101	92-93	1.0134270460759822
1101	94-95	1.0975260934322328
1101	96-97	1.1278203528922965
1101	98-99	1.1281853781728586
1101	100	1.0364648270929067
1102	1	0.356513932593014
1102	2	0.47166484631698324
1102	3	0.4975312611620808
1102	4	0.43806997355709143
1102	5	0.4353707665576678
1102	6	0.4712868364344729
1102	7	0.5195244940683494
1102	8	0.4219435695095868
1102	9	0.5192652482026148
1102	10-11	0.5709939053067359
1102	12-13	0.6082425684965571
1102	14-15	0.6798583842347057
1102	16-17	0.646023312101228
1102	18-19	0.5645899673306758
1102	20-21	0.5938196819784878
1102	22-23	0.5205717620569104
1102	24-25	0.6313282341263786
1102	26-27	0.6630649804132958
1102	28-29	0.7032901524671971
1102	30-31	0.7208077482810396
1102	32-33	0.745556702266029
1102	34-35	0.8422475679630068
1102	36-37	0.8516896079291598
1102	38-39	0.7966234106251946
1102	40-41	0.8222660316138004
1102	42-43	0.8151183096470191
1102	44-45	0.8295159309067941
1102	46-47	0.7878915067236534
1102	48-49	0.6822588429022929
1102	50-51	0.7173474886686151
1102	52-53	0.6992142007581279
1102	54-55	0.6864539535242216
1102	56-57	0.7447495512880664
1102	58-59	0.6775184775798877
1102	60-61	0.640551027404257
1102	62-63	0.6131205540584332
1102	64-65	0.6762511808520912
1102	66-67	0.6533685210580202
1102	68-69	0.6249896669668829
1102	70-71	0.6555687358189957
1102	72-73	0.6715653820641379
1102	74-75	0.7008980486519363
1102	76-77	0.6803017939786713
1102	78-79	0.641546458343285
1102	80-81	0.7110966291805241
1102	82-83	0.6239717662454041
1102	84-85	0.6264759057554592
1102	86-87	0.6797418032983273
1102	88-89	0.5630011055780351
1102	90-91	0.6865862773718163
1102	92-93	0.7569750103337256
1102	94-95	0.5962660331158709
1102	96-97	0.6198962410060531
1102	98-99	0.607413627549878
1102	100	0.6396363583600113
1103	1	0.2845242534604786
1103	2	0.3140064922300603
1103	3	0.3561548876428482
1103	4	0.3085265225850691
1103	5	0.222786428973329
1103	6	0.3168356462689985
1103	7	0.36059049584863345
1103	8	0.4393138011655324
1103	9	0.5654854908514295
1103	10-11	0.5021257560814405
1103	12-13	0.5076498429038239
1103	14-15	0.4968232190566866
1103	16-17	0.4181555931620835
1103	18-19	0.4336166935002552
1103	20-21	0.4183824797555715
1103	22-23	0.3914202132624993
1103	24-25	0.4288499959338523
1103	26-27	0.524339752402355
1103	28-29	0.5091597708368099
1103	30-31	0.4902928749090236
1103	32-33	0.43461780204141576
1103	34-35	0.5026300319168939
1103	36-37	0.5285710169534283
1103	38-39	0.4827772128504293
1103	40-41	0.454069395560019
1103	42-43	0.43283253450410086
1103	44-45	0.4057322660517073
1103	46-47	0.38917385800600357
1103	48-49	0.48279081307712346
1103	50-51	0.3597214435425684
1103	52-53	0.3236137715862668
1103	54-55	0.34927900920641264
1103	56-57	0.33073036048315885
1103	58-59	0.22156829841541992
1103	60-61	0.22017815167424715
1103	62-63	0.19526036834110272
1103	64-65	0.29613349716297677
1103	66-67	0.38008354241590325
1103	68-69	0.39999431697152943
1103	70-71	0.40888963199703454
1103	72-73	0.3806273411260932
1103	74-75	0.3811043449296676
1103	76-77	0.41506370731201514
1103	78-79	0.3286137614820177
1103	80-81	0.36822974167078115
1103	82-83	0.38708735078956025
1103	84-85	0.39427880748693056
1103	86-87	0.4020081148503536
1103	88-89	0.4322176622945939
1103	90-91	0.3215185373040761
1103	92-93	0.26816676259690553
1103	94-95	0.3056803853873653
1103	96-97	0.339589085698897
1103	98-99	0.3413751025827807
1103	100	0.27596897683548605
1104	1	0.41025423333410416
1104	2	0.4064306290740838
1104	3	0.38422736300814364
1104	4	0.4774325131485213
1104	5	0.45070871590114336
1104	6	0.5288898253949981
1104	7	0.45755004783580944
1104	8	0.5587221342755271
1104	9	0.5395658355395057
1104	10-11	0.5807340881704377
1104	12-13	0.6094354364021299
1104	14-15	0.617056746251528
1104	16-17	0.6337765434791649
1104	18-19	0.584051216420967
1104	20-21	0.552485796842312
1104	22-23	0.5351811522720524
1104	24-25	0.5583737142973462
1104	26-27	0.6789071374669788
1104	28-29	0.7721993510034579
1104	30-31	0.7788712790675376
1104	32-33	0.8375084955398364
1104	34-35	0.8414088447288606
1104	36-37	0.8336366861074964
1104	38-39	0.7452765077254142
1104	40-41	0.7322478374953647
1104	42-43	0.7473520348799525
1104	44-45	0.7610899271552185
1104	46-47	0.817805404980092
1104	48-49	0.7186984589295164
1104	50-51	0.6953877771094596
1104	52-53	0.686974786659988
1104	54-55	0.6465155572716981
1104	56-57	0.7558308200587618
1104	58-59	0.6643865522834034
1104	60-61	0.7626808924090227
1104	62-63	0.7489701937636397
1104	64-65	0.7750191571536149
1104	66-67	0.8378625964380504
1104	68-69	0.9209202833118653
1104	70-71	0.8360285601304369
1104	72-73	0.8345452199058556
1104	74-75	0.8839215267523741
1104	76-77	0.8931722621056508
1104	78-79	0.8254783098382745
1104	80-81	0.8460895309117831
1104	82-83	0.8808236199954429
1104	84-85	0.8581937636670816
1104	86-87	0.8467051280556568
1104	88-89	0.8323426066184325
1104	90-91	0.8118567803495012
1104	92-93	0.8348191098017388
1104	94-95	0.8628979174115265
1104	96-97	0.7891288592165715
1104	98-99	0.8229286748545839
1104	100	0.8701943284089033
1105	1	0.1999087527115222
1105	2	0.2269585210413183
1105	3	0.30024966446157464
1105	4	0.3088519077367806
1105	5	0.21436896214635226
1105	6	0.3141512715553745
1105	7	0.32136316206005944
1105	8	0.28414013502294466
1105	9	0.27251284938701303
1105	10-11	0.3221051275918896
1105	12-13	0.3571830704078067
1105	14-15	0.40085001339426185
1105	16-17	0.44907177512945395
1105	18-19	0.4003393576635048
1105	20-21	0.33699865956918984
1105	22-23	0.3343392168533512
1105	24-25	0.39127136294845855
1105	26-27	0.30833386992023293
1105	28-29	0.36219854297979026
1105	30-31	0.3196687984348543
1105	32-33	0.3444317433909987
1105	34-35	0.3959754410301315
1105	36-37	0.3993030026305746
1105	38-39	0.3612207843927209
1105	40-41	0.3309821946620346
1105	42-43	0.35167374950619745
1105	44-45	0.4278557113927022
1105	46-47	0.35840154969713467
1105	48-49	0.3714012981227057
1105	50-51	0.28395489862612067
1105	52-53	0.35694819763093477
1105	54-55	0.35206529112889484
1105	56-57	0.33519609221123403
1105	58-59	0.3790289513056848
1105	60-61	0.3829651652382182
1105	62-63	0.371210515155056
1105	64-65	0.340907528682898
1105	66-67	0.2845487145913097
1105	68-69	0.23829067126423098
1105	70-71	0.24857032485414265
1105	72-73	0.25638458479924253
1105	74-75	0.2823212611145891
1105	76-77	0.34315797018689764
1105	78-79	0.3571223097574716
1105	80-81	0.2889298856341753
1105	82-83	0.366958261990451
1105	84-85	0.33624847192003315
1105	86-87	0.371190012962785
1105	88-89	0.29644236724142203
1105	90-91	0.3789697622598709
1105	92-93	0.3831746301184822
1105	94-95	0.3645744907851274
1105	96-97	0.35184619233443115
1105	98-99	0.32851845638518284
1105	100	0.23696900090680728
1106	1	0.23037018889612426
1106	2	0.21972543836541547
1106	3	0.1945912325090795
1106	4	0.11895511500998879
1106	5	0.1994648833002799
1106	6	0.13755159986921228
1106	7	0.040653195507168505
1106	8	0.1074363492635868
1106	9	0.13546929537289287
1106	10-11	0.21586215016076693
1106	12-13	0.17042826502088815
1106	14-15	0.2283212392870908
1106	16-17	0.17346106585885934
1106	18-19	0.24104016105844295
1106	20-21	0.24796867247709287
1106	22-23	0.35461386996503563
1106	24-25	0.34040321857096956
1106	26-27	0.3062110296808527
1106	28-29	0.3165224116348426
1106	30-31	0.38440480208893035
1106	32-33	0.3616789486371559
1106	34-35	0.3158564431224846
1106	36-37	0.34253307831291124
1106	38-39	0.28979978962193087
1106	40-41	0.27426775586860686
1106	42-43	0.2793405904426578
1106	44-45	0.2888063288563174
1106	46-47	0.25569901050788246
1106	48-49	0.27257381290298355
1106	50-51	0.2272562495342072
1106	52-53	0.16638770101052813
1106	54-55	0.19572347996196982
1106	56-57	0.2386836291222849
1106	58-59	0.22411877861440388
1106	60-61	0.15793533039896346
1106	62-63	0.238973024423629
1106	64-65	0.2318292852776267
1106	66-67	0.23924745454980467
1106	68-69	0.2295985455347349
1106	70-71	0.22034853071863836
1106	72-73	0.13893150236963692
1106	74-75	0.1986870838228718
1106	76-77	0.2231664541777718
1106	78-79	0.25120300954584707
1106	80-81	0.1611652651635609
1106	82-83	0.19783514224770116
1106	84-85	0.1246461572664046
1106	86-87	0.1903986769702506
1106	88-89	0.1883770436836123
1106	90-91	0.1809785201565859
1106	92-93	0.2518973724757103
1106	94-95	0.2710391211073997
1106	96-97	0.21040139313092254
1106	98-99	0.24299814443851986
1106	100	0.308205603683259
1107	1	-0.11421204593807488
1107	2	0.015318882284169888
1107	3	-0.005830679640730807
1107	4	-0.04926349083407899
1107	5	0.056113434485766334
1107	6	0.07075461873101574
1107	7	-0.002024793918970147
1107	8	-0.012482458577743216
1107	9	-0.07622216410126015
1107	10-11	-0.03965142145732159
1107	12-13	-0.021724318920668395
1107	14-15	-0.05875340605636126
1107	16-17	-0.028455886561978616
1107	18-19	-0.06308289591456173
1107	20-21	-0.21849857765528924
1107	22-23	-0.10980237736406906
1107	24-25	-0.05808618305530899
1107	26-27	-0.045613403643514516
1107	28-29	-0.12274156967379923
1107	30-31	-0.07740208351440714
1107	32-33	-0.015714784052761388
1107	34-35	-0.021454836538829625
1107	36-37	5.17561747656714E-4
1107	38-39	-0.026062752943182943
1107	40-41	-0.05274420728007101
1107	42-43	-0.05854090720046656
1107	44-45	-0.05739267058316244
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2208	80-81	1.1080327173106639
2208	82-83	1.125613058735869
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2216	94-95	-1.7642095067980996
2216	96-97	-1.9125616120437918
2216	98-99	-2.009105029626486
2216	100	-2.0650983386781654
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	82962.0
3	16420.0
4	11648.0
5	10902.0
6	8152.0
7	8860.0
8	10259.0
9	10208.0
10	9816.0
11	10349.0
12	11893.0
13	13081.0
14	12665.0
15	11856.0
16	11502.0
17	11195.0
18	10461.0
19	9879.0
20	9454.0
21	9891.0
22	13079.0
23	26309.0
24	43856.0
25	45545.0
26	40654.0
27	28641.0
28	30609.0
29	33995.0
30	40014.0
31	49000.0
32	64897.0
33	87935.0
34	125998.0
35	206713.0
36	370642.0
37	619598.0
38	702129.0
39	239438.0
40	1788.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.69251794247826	21.58890449814289	13.336409466653343	22.3821680927255
2	15.851236868083404	23.070243814190093	38.024080663790606	23.054438653935893
3	17.43547559653867	30.799946837188237	28.525995733259833	23.238581833013257
4	12.030012823732298	20.27253451745768	38.03926537353896	29.658187285271058
5	12.54001904455878	41.91618565440526	32.45797592130339	13.085819379732566
6	27.125606286273907	40.9614566349181	18.344011068837023	13.568926009970966
7	24.865746027568232	36.39005150715493	22.173384454067588	16.570818011209248
8	24.092469270576004	37.34766725457035	20.172563500618654	18.387299974234995
9	25.99565097134729	19.522135863550613	19.84248404708498	34.63972911801712
10-11	23.115129741014332	30.145286554878975	26.752110265085673	19.987473439021024
12-13	23.895558001797998	28.883111446226735	26.135497158501813	21.08583339347345
14-15	22.163130699773014	29.110130872519385	24.4357904354678	24.290947992239804
16-17	21.45895249082958	31.419331853614267	25.013511117322867	22.108204538233277
18-19	21.550191310890238	30.29752541641182	26.886029521015786	21.266253751682154
20-21	24.340322758142346	28.67039502751696	25.73439249608055	21.254889718260138
22-23	23.46876997073761	28.966986503250997	25.778934282251893	21.785309243759496
24-25	22.446055945659023	29.120907111109222	26.20196695744006	22.23106998579169
26-27	22.421727770660745	29.395668539881715	26.135856366454814	22.04674732300273
28-29	22.165481879101705	28.970774514391668	26.847643252948032	22.016100353558592
30-31	22.831257492343155	29.40773466157549	25.947419139840637	21.813588706240715
32-33	22.03559554882567	29.282044533295803	26.437052235040863	22.245307682837666
34-35	22.65679672062732	28.448959652129957	26.72530028968489	22.168943337557838
36-37	22.599666328466935	29.12500534730021	25.99263035901529	22.28269796521757
38-39	22.35062092360202	29.157627960485822	26.33521678036687	22.15653433554529
40-41	22.49242642686379	28.69024943073703	26.32729787776676	22.49002626463242
42-43	21.938527763345963	29.134508030420342	26.710360504367152	22.216603701866543
44-45	22.30981816566867	28.874670059331358	26.31041510397601	22.50509667102397
46-47	22.40332652688688	28.990155416219153	26.311117192247767	22.295400864646197
48-49	21.955410537136714	28.882164443441567	26.40857684094892	22.7538481784728
50-51	22.29758876763262	28.88108681958258	26.158421156956567	22.662903255828233
52-53	22.26310480414513	29.1347856002022	26.036225142401463	22.565884453251208
54-55	22.21358308953454	29.192373166120944	26.115757048721335	22.478286695623183
56-57	22.360548125212055	29.07625103149829	25.92741778791252	22.635783055377132
58-59	22.35293944766226	28.252897420331756	26.496648099969534	22.89751503203645
60-61	22.63349718658535	28.979836351387668	25.89539929719331	22.491267164833673
62-63	22.01691673526994	28.97691370486103	26.198603464789294	22.807566095079732
64-65	22.304694930350383	28.681143293790516	26.110552796638466	22.903608979220632
66-67	22.020525142434117	28.801407964554667	26.102058163604852	23.076008729406364
68-69	22.51086032590611	28.992669871890115	26.165703281821823	22.330766520381946
70-71	22.45887313852724	28.784247620982057	26.131986717143	22.6248925233477
72-73	22.266027450671768	28.877641688760676	26.11449982088585	22.741831039681703
74-75	22.20512537500494	28.780084074254162	26.173067044858218	22.841723505882683
76-77	22.242238087602985	28.809081952641368	25.91027377197414	23.03840618778151
78-79	22.46824520057356	28.552493180763566	26.30182676837259	22.67743485029029
80-81	22.114441694508006	29.14613330598999	26.214931099016326	22.52449390048568
82-83	22.076766419373477	29.099453203232418	26.34403115708035	22.479749220313757
84-85	22.051701045479696	28.487676741354516	26.32756948788296	23.133052725282823
86-87	22.526286158775648	28.913305149337514	26.03542875877865	22.524979933108185
88-89	22.15161030494584	28.81706433561889	26.211518877199214	22.819806482236054
90-91	22.156193520355572	28.8190546696425	25.905792871545337	23.118958938456586
92-93	22.093391203881346	28.964871105665562	26.172435379756692	22.769302310696396
94-95	22.181950230436417	28.29681804095247	26.422140313947374	23.099091414663743
96-97	22.094757432467734	29.089996211923296	26.070360259156693	22.744886096452273
98-99	21.642229342325496	29.037987617812753	26.16133418173702	23.158448858124732
100	21.650420790454486	28.925976083309685	26.304114050851457	23.11948907538437
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26389.0
1	18107.5
2	7408.5
3	3684.0
4	1934.5
5	1326.0
6	1144.5
7	1151.0
8	1304.0
9	1375.5
10	1123.0
11	885.5
12	896.5
13	940.0
14	970.5
15	1074.5
16	1263.5
17	1609.0
18	2030.0
19	2596.0
20	3388.5
21	4349.0
22	5507.5
23	7287.0
24	9776.0
25	12503.0
26	16003.0
27	21171.5
28	27094.5
29	33157.0
30	38927.0
31	44116.5
32	48843.5
33	53698.0
34	59108.5
35	64553.0
36	70338.0
37	77339.0
38	84175.5
39	87848.5
40	93566.0
41	100242.0
42	104877.5
43	112770.5
44	115905.5
45	113174.5
46	113594.5
47	114729.5
48	115268.5
49	117057.5
50	119333.0
51	113303.5
52	102487.5
53	94355.0
54	89086.5
55	86739.0
56	77246.0
57	65861.0
58	62088.5
59	59121.5
60	52966.5
61	44859.0
62	37194.0
63	28629.5
64	20772.5
65	16730.5
66	13755.0
67	11304.0
68	8560.0
69	5779.5
70	4144.5
71	3360.5
72	2616.5
73	1845.5
74	1365.5
75	1069.0
76	751.5
77	550.5
78	401.5
79	280.5
80	196.5
81	150.5
82	132.5
83	101.0
84	89.0
85	92.0
86	76.5
87	60.5
88	57.5
89	52.5
90	44.0
91	34.5
92	29.5
93	25.5
94	24.5
95	20.5
96	19.0
97	18.0
98	18.0
99	22.5
100	47.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.285868791784457E-4
2	6.531053690812734E-5
3	6.531053690812734E-5
4	6.531053690812734E-5
5	3.265526845406367E-5
6	6.531053690812734E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	1.6327634227031836E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.1429343958922285E-4
84-85	4.7350139258392325E-4
86-87	0.0011429343958922285
88-89	0.003053267600454953
90-91	0.002367506962919616
92-93	0.0018286950334275656
94-95	0.00195931610724382
96-97	0.0017307292280653746
98-99	0.002334851694465552
100	0.0026124214763250937
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3062293.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.529014162690366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.0987322097016	38.45193917586794
2	13.197273683449371	13.336904577198283
3	4.558036429250798	6.909392618630167
4	2.0423889810314853	4.127996069930507
5	1.1186171196852894	2.8261310141602944
6	0.6512595397946396	1.9744501505922334
7	0.4553764727732006	1.6106806969479126
8	0.2964184968294365	1.198218753950239
9	0.21947177607668852	0.9980723233520825
>10	1.1497400986019028	10.625338020384389
>50	0.10997998233378513	3.889936325013342
>100	0.09332589726483907	9.131115759255112
>500	0.007374080728472652	2.4733456492880013
>1k	0.0019405475599759752	1.467736969859907
>5k	0.0	0.0
>10k+	6.468491866586584E-5	0.9787418955697439
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29945	0.9778620138569366	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.265526845406367E-5	0.0
5	0.0	0.0	0.0	3.265526845406367E-5	0.0
6	0.0	0.0	0.0	3.265526845406367E-5	0.0
7	0.0	0.0	0.0	3.265526845406367E-5	3.265526845406367E-5
8	0.0	0.0	0.0	3.265526845406367E-5	3.265526845406367E-5
9	0.0	0.0	0.0	9.796580536219101E-5	3.265526845406367E-5
10-11	0.0	0.0	0.0	1.3062107381625469E-4	3.265526845406367E-5
12-13	0.0	0.0	0.0	1.3062107381625469E-4	3.265526845406367E-5
14-15	0.0	0.0	0.0	1.4694870804328653E-4	3.265526845406367E-5
16-17	0.0	0.0	0.0	1.9593161072438203E-4	3.265526845406367E-5
18-19	0.0	0.0	0.0	3.4288031876766856E-4	3.265526845406367E-5
20-21	0.0	0.0	0.0	4.7350139258392325E-4	3.265526845406367E-5
22-23	0.0	0.0	0.0	0.0011755896643462923	3.265526845406367E-5
24-25	0.0	0.0	0.0	0.002285868791784457	3.265526845406367E-5
26-27	0.0	0.0	0.0	0.0033308373823144944	3.265526845406367E-5
28-29	0.0	0.0	0.0	0.007494384110207612	3.265526845406367E-5
30-31	0.0	0.0	0.0	0.016131702616307454	3.265526845406367E-5
32-33	0.0	0.0	0.0	0.02792025452822444	3.265526845406367E-5
34-35	0.0	0.0	0.0	0.045407150785375536	3.265526845406367E-5
36-37	0.0	0.0	0.0	0.06978430868633406	3.265526845406367E-5
38-39	0.0	0.0	0.0	0.10697865945551259	3.265526845406367E-5
40-41	0.0	0.0	0.0	0.1520755851905745	3.265526845406367E-5
42-43	0.0	0.0	0.0	0.19454376181508432	3.265526845406367E-5
44-45	0.0	0.0	0.0	0.24179593526811444	3.265526845406367E-5
46-47	0.0	0.0	0.0	0.29633023358640076	3.265526845406367E-5
48-49	0.0	0.0	0.0	0.35744456849818096	3.265526845406367E-5
50-51	0.0	0.0	0.0	0.42709825611069874	3.265526845406367E-5
52-53	0.0	0.0	0.0	0.49652335684403814	3.265526845406367E-5
54-55	0.0	0.0	0.0	0.567270995949767	3.265526845406367E-5
56-57	0.0	0.0	0.0	0.649317357940602	3.265526845406367E-5
58-59	0.0	0.0	0.0	0.7422215966924132	3.265526845406367E-5
60-61	0.0	0.0	0.0	0.8477307690674929	3.265526845406367E-5
62-63	0.0	0.0	0.0	0.9584321291267688	3.265526845406367E-5
64-65	0.0	0.0	0.0	1.067370104689525	3.265526845406367E-5
66-67	0.0	0.0	0.0	1.1891416007547286	3.265526845406367E-5
68-69	0.0	0.0	0.0	1.3300980670366944	3.265526845406367E-5
70-71	0.0	0.0	0.0	1.4948928792901266	3.265526845406367E-5
72-73	0.0	0.0	0.0	1.6719497448480598	3.265526845406367E-5
74-75	0.0	0.0	0.0	1.853088518962751	3.265526845406367E-5
76-77	0.0	0.0	0.0	2.0405624151575306	3.265526845406367E-5
78-79	0.0	0.0	0.0	2.255009563095367	3.265526845406367E-5
80-81	0.0	0.0	0.0	2.4974586037325626	3.265526845406367E-5
82-83	0.0	0.0	0.0	2.76250509013997	3.265526845406367E-5
84-85	0.0	0.0	0.0	3.035797031832029	3.265526845406367E-5
86-87	0.0	0.0	0.0	3.320469334580329	3.265526845406367E-5
88	0.0	0.0	0.0	3.546133567232136	3.265526845406367E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4915	0.0	44.083096	1
GTATCAA	8925	0.0	39.811474	1
ATCAACG	11080	0.0	30.286783	3
TCAACGC	11175	0.0	30.02931	4
CAACGCA	12755	0.0	26.383188	5
TATCAAC	13505	0.0	26.170858	2
AACGCAG	13290	0.0	25.391838	6
GTACATG	20265	0.0	25.32624	1
TACATGG	19565	0.0	25.03125	2
ACGCAGA	13350	0.0	24.503191	7
CGCAGAG	13195	0.0	24.1855	8
ACATGGG	20835	0.0	22.422684	3
GCAGAGT	14065	0.0	21.319431	9
CGTCACT	515	0.0	20.990139	1
CATGGGG	11015	0.0	20.60902	4
GTACAAA	2465	0.0	20.401474	1
ATGCGGG	460	0.0	19.4129	8
CGCAGAA	600	0.0	18.799864	2
CATGGGA	6155	0.0	18.55551	4
GTCGGGA	1455	0.0	18.412237	2
>>END_MODULE