FastQCFastQC Report
Wed 25 May 2016
SRR2431363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431363_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062293
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286240.9347244042291185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205560.6712616983417328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153150.5001154363739851No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36410.11889783244124583No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35840.1170364821393642No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33810.11040746264318928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94650.064.4593351
GTATCAA210350.050.210241
ATCAACG287000.036.6009143
TCAACGC291000.036.0978054
TATCAAC301150.035.0060162
CAACGCA306100.034.315415
AACGCAG313700.033.484056
ACGCAGA341350.030.6065627
CGCAGAG343050.030.2356938
GCAGAGT362900.028.2321879
GTGGTAT29100.027.9437621
TGGTATC31150.026.2535172
GTACATG192750.024.9466921
TACATGG188000.024.849912
ACATGGG193400.023.2082923
AGAGTAC359350.023.20776710-11
AGTACTT259700.023.1730212-13
GAGTACT259550.022.27199412-13
CAGAGTA364500.021.8870510-11
GTACTTT268200.021.37844314-15
Produced by FastQC (version 0.11.5)