##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431363_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3062293
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75004775833011	34.0	31.0	34.0	31.0	34.0
2	32.85455016877876	34.0	31.0	34.0	31.0	34.0
3	32.922251724443086	34.0	31.0	34.0	31.0	34.0
4	36.33099249483965	37.0	37.0	37.0	35.0	37.0
5	36.300667179789784	37.0	37.0	37.0	35.0	37.0
6	36.343497176788766	37.0	37.0	37.0	35.0	37.0
7	36.21104446896492	37.0	37.0	37.0	35.0	37.0
8	36.260224936020165	37.0	37.0	37.0	35.0	37.0
9	38.06850389561025	39.0	39.0	39.0	35.0	39.0
10-11	38.060262685510494	39.0	39.0	39.0	35.0	39.0
12-13	38.038906303217885	39.0	39.0	39.0	35.0	39.0
14-15	39.45706452648391	41.0	39.0	41.0	36.0	41.0
16-17	39.4773199364006	41.0	39.0	41.0	36.5	41.0
18-19	39.590009512479696	41.0	40.0	41.0	37.0	41.0
20-21	39.593874100224895	41.0	40.0	41.0	37.0	41.0
22-23	39.511572863863776	41.0	40.0	41.0	36.0	41.0
24-25	39.46823491416399	41.0	39.0	41.0	36.0	41.0
26-27	39.35790631399412	41.0	39.0	41.0	35.5	41.0
28-29	39.24843213892335	41.0	39.0	41.0	35.0	41.0
30-31	38.997228384089965	41.0	38.5	41.0	35.0	41.0
32-33	38.701080203625196	41.0	38.0	41.0	35.0	41.0
34-35	38.54818823672326	41.0	38.0	41.0	35.0	41.0
36-37	38.3929650102064	40.5	38.0	41.0	35.0	41.0
38-39	38.227096329449864	40.0	38.0	41.0	35.0	41.0
40-41	38.05535312917477	40.0	38.0	41.0	34.0	41.0
42-43	37.825775162598745	40.0	37.5	41.0	34.0	41.0
44-45	37.472825428526924	40.0	37.0	41.0	33.0	41.0
46-47	36.92704404836506	40.0	37.0	41.0	32.0	41.0
48-49	36.45506537094916	40.0	37.0	41.0	30.5	41.0
50-51	36.20269092474169	40.0	36.0	41.0	29.5	41.0
52-53	36.031006993778846	40.0	36.0	41.0	29.0	41.0
54-55	35.8444374852439	40.0	35.0	41.0	29.0	41.0
56-57	35.63712110500204	39.5	35.0	41.0	28.0	41.0
58-59	35.40594368337713	39.0	35.0	41.0	28.0	41.0
60-61	35.17194876519001	39.0	35.0	41.0	28.0	41.0
62-63	34.91226231454665	38.0	35.0	41.0	27.0	41.0
64-65	34.61476987995597	37.5	35.0	40.5	26.5	41.0
66-67	34.308564856465395	37.0	35.0	40.0	26.0	41.0
68-69	34.02632831019109	36.0	35.0	39.5	26.0	41.0
70-71	33.76895042375109	36.0	35.0	39.0	26.0	41.0
72-73	33.52119604492451	35.5	35.0	39.0	26.0	41.0
74-75	33.24525167905227	35.0	35.0	38.0	26.0	40.5
76-77	32.96359362085862	35.0	34.0	37.0	26.0	39.5
78-79	32.68657750907572	35.0	34.0	37.0	25.0	39.0
80-81	32.431987892732664	35.0	34.0	36.0	25.0	39.0
82-83	32.17694257211835	35.0	34.0	36.0	25.0	37.5
84-85	31.96821662721366	35.0	34.0	36.0	24.0	37.0
86-87	31.795142398196383	35.0	34.0	35.0	24.0	37.0
88-89	31.622694333951713	35.0	34.0	35.0	23.5	36.0
90-91	31.459581757852696	35.0	34.0	35.0	22.0	36.0
92-93	31.34427976029727	35.0	34.0	35.0	20.0	36.0
94-95	31.23866494812874	35.0	34.0	35.0	20.0	36.0
96-97	31.131690370581783	35.0	34.0	35.0	18.5	35.5
98-99	31.029772951183965	35.0	34.0	35.0	17.0	35.0
100	30.933621635813424	35.0	34.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2785190388863725
1101	2	0.28176162040998776
1101	3	0.2680785784301918
1101	4	0.1965085929412851
1101	5	0.22847779291839743
1101	6	0.2413611665189208
1101	7	0.2752398877644069
1101	8	0.3203061224503614
1101	9	0.4038639927879899
1101	10-11	0.3610293268535685
1101	12-13	0.3498947141462523
1101	14-15	0.4476922413873652
1101	16-17	0.4467071094167636
1101	18-19	0.44907283315134805
1101	20-21	0.4101548913111088
1101	22-23	0.44460401610355405
1101	24-25	0.4217505448362644
1101	26-27	0.43754635049470636
1101	28-29	0.43888792996283854
1101	30-31	0.44454161685774807
1101	32-33	0.486863296189469
1101	34-35	0.4260731050352433
1101	36-37	0.40752917454834403
1101	38-39	0.40326983034902497
1101	40-41	0.4188427827969363
1101	42-43	0.388590133519763
1101	44-45	0.38561370221978564
1101	46-47	0.37988565306795863
1101	48-49	0.30564230014598337
1101	50-51	0.30561311907583644
1101	52-53	0.2490606529547108
1101	54-55	0.28417370422015154
1101	56-57	0.23313318477962497
1101	58-59	0.2516594829730181
1101	60-61	0.24058552562217272
1101	62-63	0.1980745871183629
1101	64-65	0.2599880491914561
1101	66-67	0.2778737443775299
1101	68-69	0.22185886949549882
1101	70-71	0.22561042223664884
1101	72-73	0.1921146658361934
1101	74-75	0.23280048502430617
1101	76-77	0.22130661451825517
1101	78-79	0.19628781032500342
1101	80-81	0.2550640400383415
1101	82-83	0.2344145149039818
1101	84-85	0.22979140135900877
1101	86-87	0.2067940684673637
1101	88-89	0.22471241789297736
1101	90-91	0.29439396519985905
1101	92-93	0.2936100307821228
1101	94-95	0.29798054469083013
1101	96-97	0.2937354339998528
1101	98-99	0.3694537456569158
1101	100	0.4148360555021213
1102	1	0.19956393268221717
1102	2	0.20516671494812044
1102	3	0.24233234343230237
1102	4	0.17163112952445658
1102	5	0.1959048759535733
1102	6	0.16101394411600012
1102	7	0.19404893886157737
1102	8	0.22098090849381435
1102	9	0.3153698548548647
1102	10-11	0.29343438756458795
1102	12-13	0.2571202525829719
1102	14-15	0.36463299448319475
1102	16-17	0.40514317095090746
1102	18-19	0.35151861000856144
1102	20-21	0.308702742811775
1102	22-23	0.3100102863510372
1102	24-25	0.35817290161525506
1102	26-27	0.36299354071849876
1102	28-29	0.29002907493287466
1102	30-31	0.33543152822201705
1102	32-33	0.35030032916917975
1102	34-35	0.3675983699926846
1102	36-37	0.29854295606503456
1102	38-39	0.32114484086579154
1102	40-41	0.3799763405168477
1102	42-43	0.34738354824635564
1102	44-45	0.3824748271364129
1102	46-47	0.32769349289393546
1102	48-49	0.3490587614646685
1102	50-51	0.37407227629476125
1102	52-53	0.32396338913525113
1102	54-55	0.2939350517019008
1102	56-57	0.29624619295625365
1102	58-59	0.3233563945567184
1102	60-61	0.3853316205410735
1102	62-63	0.3768781434765174
1102	64-65	0.38694275814376766
1102	66-67	0.3808233935729177
1102	68-69	0.4014496111855195
1102	70-71	0.3808901421827642
1102	72-73	0.38498455891646444
1102	74-75	0.3384412878199825
1102	76-77	0.3719137463165296
1102	78-79	0.3148163131452293
1102	80-81	0.3048138711195634
1102	82-83	0.3449124371380279
1102	84-85	0.3430680807672246
1102	86-87	0.2955078116466474
1102	88-89	0.3328679203081464
1102	90-91	0.36502418400930026
1102	92-93	0.37596319071242235
1102	94-95	0.3430541555453459
1102	96-97	0.35921834653245455
1102	98-99	0.4202382855343245
1102	100	0.4769010191900058
1103	1	0.20478800933486951
1103	2	0.19792797556652175
1103	3	0.24462589929729006
1103	4	0.18671246603436487
1103	5	0.16918427780440481
1103	6	0.2527527501405231
1103	7	0.1919312581724668
1103	8	0.2176414230114716
1103	9	0.31934560883061636
1103	10-11	0.3135693898424421
1103	12-13	0.31719311622726565
1103	14-15	0.3956069540285867
1103	16-17	0.3981195478558561
1103	18-19	0.39715998600708957
1103	20-21	0.38660615107232843
1103	22-23	0.44389894702541
1103	24-25	0.37434463207270596
1103	26-27	0.38304009647934123
1103	28-29	0.3985624334662319
1103	30-31	0.4519396179443902
1103	32-33	0.4103931481905647
1103	34-35	0.4646005741377479
1103	36-37	0.4588867949802946
1103	38-39	0.45560652889890463
1103	40-41	0.4458956475075837
1103	42-43	0.41676657298651776
1103	44-45	0.45045494868796254
1103	46-47	0.37328392241293074
1103	48-49	0.35081847751009576
1103	50-51	0.3667805547173302
1103	52-53	0.4095990908423772
1103	54-55	0.4146048420145476
1103	56-57	0.34845195051914857
1103	58-59	0.3599375204235642
1103	60-61	0.31218814561189134
1103	62-63	0.36300907603602184
1103	64-65	0.36333594882266596
1103	66-67	0.35915772012153013
1103	68-69	0.390857002378624
1103	70-71	0.3778624962979791
1103	72-73	0.4246123485442581
1103	74-75	0.41004343085069905
1103	76-77	0.3719480856365891
1103	78-79	0.3708991192280351
1103	80-81	0.4357713413627451
1103	82-83	0.4603213204040557
1103	84-85	0.4486464631313183
1103	86-87	0.3749161499835516
1103	88-89	0.41339335797644594
1103	90-91	0.4607340565048865
1103	92-93	0.482027734276965
1103	94-95	0.55047213974904
1103	96-97	0.5310404647831426
1103	98-99	0.5120904756008002
1103	100	0.5289354283672729
1104	1	0.11839397399055684
1104	2	0.19704614175650903
1104	3	0.19250244562687158
1104	4	0.06374882240773161
1104	5	0.09277261752534116
1104	6	0.14045161772889259
1104	7	0.1202801425434501
1104	8	0.18297944271413513
1104	9	0.2594446198688516
1104	10-11	0.20660985061770987
1104	12-13	0.22922692649311927
1104	14-15	0.2990870171771576
1104	16-17	0.29718319288635087
1104	18-19	0.2913608336885929
1104	20-21	0.2461800386020201
1104	22-23	0.24517641659017642
1104	24-25	0.26986715842395625
1104	26-27	0.23164440839856582
1104	28-29	0.26372570978973187
1104	30-31	0.31534611836746507
1104	32-33	0.2814567319613417
1104	34-35	0.32794287946899914
1104	36-37	0.36837546767339546
1104	38-39	0.33910428079445154
1104	40-41	0.31269882127208604
1104	42-43	0.3543187618094308
1104	44-45	0.30985092775244993
1104	46-47	0.3305909446923323
1104	48-49	0.37531282310941805
1104	50-51	0.40923602336064135
1104	52-53	0.358441269187324
1104	54-55	0.36131676430657933
1104	56-57	0.3348646956059227
1104	58-59	0.3617784263528634
1104	60-61	0.38256378261736046
1104	62-63	0.3986675734624683
1104	64-65	0.3968446636573475
1104	66-67	0.39297914035179105
1104	68-69	0.4205825205019309
1104	70-71	0.43737083591681625
1104	72-73	0.4335236993340601
1104	74-75	0.4480570075383028
1104	76-77	0.4037195766345931
1104	78-79	0.4128295154125823
1104	80-81	0.4217312749469926
1104	82-83	0.47304213301437414
1104	84-85	0.40857861798060213
1104	86-87	0.41758909066755123
1104	88-89	0.4150083686522237
1104	90-91	0.4310283712190923
1104	92-93	0.4302261239118188
1104	94-95	0.3765004034568662
1104	96-97	0.4624126699675024
1104	98-99	0.4630240100702423
1104	100	0.4174500203072782
1105	1	-0.06247550998532603
1105	2	-0.027701552850942335
1105	3	0.04224958471804996
1105	4	-0.09340577272190131
1105	5	-0.14153869379607897
1105	6	-0.11809151558491493
1105	7	-0.5442927664427799
1105	8	-0.3950695336208767
1105	9	-0.38836836305154776
1105	10-11	-0.34375747189483263
1105	12-13	-0.20928678970877002
1105	14-15	-0.19198571000374898
1105	16-17	-0.14513020414618438
1105	18-19	-0.19768403182596472
1105	20-21	-0.1855425163688409
1105	22-23	-0.1608984569036167
1105	24-25	-0.18297433258172902
1105	26-27	-0.25866579694045555
1105	28-29	-0.24981636299300902
1105	30-31	-0.20423987050200765
1105	32-33	-0.15006831172244262
1105	34-35	-0.11492605909089804
1105	36-37	-0.12263509218600888
1105	38-39	-0.1235142966798719
1105	40-41	-0.1163963942177304
1105	42-43	-0.14855037875638288
1105	44-45	-0.08045397493120277
1105	46-47	0.006316221692948432
1105	48-49	-0.07853372309438811
1105	50-51	-0.09068489132215518
1105	52-53	-0.05253025302828007
1105	54-55	5.740642954705777E-4
1105	56-57	0.030678905712285598
1105	58-59	-0.02192911452808488
1105	60-61	-0.04649355676268385
1105	62-63	-0.016484158233261326
1105	64-65	-0.02807375120220712
1105	66-67	0.010788916428225548
1105	68-69	-0.02395561821553116
1105	70-71	-0.042600655997901526
1105	72-73	-0.04795692829832632
1105	74-75	-0.10526497979591909
1105	76-77	-0.10372860884723423
1105	78-79	-0.03829184469327629
1105	80-81	-0.0312833856325625
1105	82-83	-0.03142878823361883
1105	84-85	-0.03213860403400304
1105	86-87	-0.0018957184873613642
1105	88-89	0.013708006051601274
1105	90-91	0.08731951729967236
1105	92-93	0.06669362115948729
1105	94-95	0.0436119713258023
1105	96-97	0.05572937989710525
1105	98-99	0.06317301468607894
1105	100	0.06553212561296462
1106	1	0.06897331197699685
1106	2	0.08919434203190946
1106	3	0.10576434708607252
1106	4	0.06837513581705679
1106	5	0.09313088209393783
1106	6	0.0883411295133456
1106	7	0.15516600830250127
1106	8	0.10993731775684523
1106	9	0.14749501689428968
1106	10-11	0.14549323983121099
1106	12-13	0.13444258105786844
1106	14-15	0.1581219538369112
1106	16-17	0.1683734797754255
1106	18-19	0.18956299243335906
1106	20-21	0.17397107499573394
1106	22-23	0.16873961981464447
1106	24-25	0.1601895942801903
1106	26-27	0.1915991855038257
1106	28-29	0.21807097587740287
1106	30-31	0.22920800697028199
1106	32-33	0.18294017823912867
1106	34-35	0.15027949343577518
1106	36-37	0.19828140179682663
1106	38-39	0.10316743947267781
1106	40-41	0.17393269581407367
1106	42-43	0.1977167492214491
1106	44-45	0.18634657067879346
1106	46-47	0.13113343898871932
1106	48-49	0.08357426782865929
1106	50-51	0.08180180801169712
1106	52-53	0.07631802608550231
1106	54-55	-0.02883454631155047
1106	56-57	0.0246031358558767
1106	58-59	-0.0030830912192172377
1106	60-61	0.015855659750926065
1106	62-63	0.03920411322310002
1106	64-65	0.017079687379627728
1106	66-67	0.0070959687946299255
1106	68-69	-0.01705262659653073
1106	70-71	-0.036903652393462494
1106	72-73	-0.0433250881623124
1106	74-75	-0.015047989647946736
1106	76-77	-0.0582369394657718
1106	78-79	-0.061133058976828636
1106	80-81	-0.06951174567171137
1106	82-83	-0.052189400312421697
1106	84-85	-0.04353956151030758
1106	86-87	-0.023616680270034607
1106	88-89	-0.008167564196849497
1106	90-91	0.03197372143714006
1106	92-93	0.011585720356688256
1106	94-95	0.061449389550539024
1106	96-97	-0.028218025889923837
1106	98-99	9.535909182929458E-4
1106	100	0.04775982533006484
1107	1	-0.06467939258286748
1107	2	-0.03018398764477581
1107	3	-0.019201258569601976
1107	4	-0.1313797196074944
1107	5	-0.13649467025864936
1107	6	-0.09883280012052609
1107	7	-0.08929504292138546
1107	8	-0.019394019321985922
1107	9	-0.009126289177707747
1107	10-11	-0.04191959147435398
1107	12-13	-0.009064878904908369
1107	14-15	-0.009121444077301533
1107	16-17	-0.030950516975806863
1107	18-19	-0.021358611319463705
1107	20-21	0.02284997605786998
1107	22-23	0.01777305732336032
1107	24-25	0.019916708108361547
1107	26-27	0.01370493906470216
1107	28-29	0.011431844348884113
1107	30-31	0.07289681837178819
1107	32-33	0.0146373915076623
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2216	44-45	-1.1256509452416168
2216	46-47	-1.036037689527923
2216	48-49	-1.067331416257339
2216	50-51	-0.8937437784219924
2216	52-53	-0.949187605039711
2216	54-55	-1.0409214400662492
2216	56-57	-1.0070405817195578
2216	58-59	-1.1129332831542271
2216	60-61	-1.0704822327995487
2216	62-63	-1.1412480476844777
2216	64-65	-1.133430638233527
2216	66-67	-1.0168821498493372
2216	68-69	-1.1179157429784468
2216	70-71	-1.074641032653588
2216	72-73	-1.1098646081005015
2216	74-75	-1.0180220346934732
2216	76-77	-0.8875550946502457
2216	78-79	-0.9102066409024232
2216	80-81	-1.011640467313967
2216	82-83	-1.0113940393010807
2216	84-85	-0.9822996185813722
2216	86-87	-0.9836930161213537
2216	88-89	-0.9355227757565565
2216	90-91	-0.9659838503024574
2216	92-93	-1.116090007367184
2216	94-95	-1.0416677330552275
2216	96-97	-1.1765368297830783
2216	98-99	-1.1603847450004885
2216	100	-1.2494409036717506
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35.0
3	418.0
4	246.0
5	351.0
6	480.0
7	804.0
8	1344.0
9	2036.0
10	2843.0
11	4711.0
12	8248.0
13	12720.0
14	12752.0
15	17190.0
16	23709.0
17	40646.0
18	49659.0
19	21746.0
20	10715.0
21	8244.0
22	7769.0
23	7946.0
24	8790.0
25	10468.0
26	13011.0
27	16289.0
28	19870.0
29	24782.0
30	31445.0
31	39269.0
32	50104.0
33	64478.0
34	91848.0
35	161195.0
36	296241.0
37	664106.0
38	934133.0
39	395093.0
40	6559.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.30774498786585	20.60189698365686	14.996673763291476	23.093684265185814
2	14.611278088438334	21.99630211619271	39.728216642958934	23.664203152410025
3	15.576611782670216	29.889327451435648	31.26013747286741	23.273923293026723
4	10.673669848773457	18.841287729485764	39.78450977017706	30.700532651563716
5	11.852360306476225	38.71781700836595	34.366502486861975	15.063320198295852
6	27.145247042004144	39.773659803291196	18.556127712142505	14.524965442562157
7	24.365075451630528	34.592444289295635	23.537329706856923	17.505150552216918
8	25.847885881592646	32.986850049946234	20.81789691580786	20.347367152653256
9	27.701986713877474	14.532312877964323	21.516425763308735	36.249274644849464
10-11	24.10353940658193	26.587984885835546	28.162262722737502	21.146212984845018
12-13	26.040535637837397	25.57459393990059	28.30932572422038	20.075544698041632
14-15	23.317069921134262	25.283292617656116	28.31037069281091	23.089266768398716
16-17	20.42376415320154	28.384449169298954	28.373379033293027	22.81840764420648
18-19	20.25379348089814	25.653733982999015	33.51852680328108	20.573945732821777
20-21	22.45717506456763	24.60269151253652	33.00510434501206	19.935029077883794
22-23	21.652652100225648	24.840772755029732	33.081076579945076	20.425498564799547
24-25	20.83400616825668	25.063436971953845	33.33237544498948	20.770181414799996
26-27	20.772539771072235	25.381366669758048	33.26504534535751	20.58104821381221
28-29	20.63990890400044	25.008947628290702	33.87488799136848	20.47625547634038
30-31	21.305310959105636	25.372655701612235	32.94215499192014	20.37987834736199
32-33	20.53917443323559	25.32330904657917	33.34268946493051	20.794827055254732
34-35	21.170332606769243	24.740233879723245	33.27034339991173	20.819090113595784
36-37	21.2304365368284	25.351175297741168	32.38351482431724	21.03487334111319
38-39	21.074197453416982	25.389790612761132	32.591328320196354	20.944683613625536
40-41	21.153158172229976	25.180389658971247	32.262689078292155	21.403763090506615
42-43	20.882359328193044	25.50489774199179	32.44309751709389	21.16964541272128
44-45	21.386980089012138	25.312193957506814	31.673126452553802	21.62769950092725
46-47	21.709303740061216	25.634445366989066	30.83431506776621	21.82193582518351
48-49	21.76774001825476	25.78517511017352	29.85116982315089	22.595915048420835
50-51	22.256601283135407	26.014963762927728	29.132836174014358	22.595598779922504
52-53	22.100579533049256	26.472221959165893	28.900908567534195	22.526289940250656
54-55	22.178180963203886	26.53903300808548	28.937784918426797	22.345001110283842
56-57	22.341295071533512	26.142722182328082	28.815068947196377	22.700913798942025
58-59	22.220500552938855	25.318786842520762	29.443761310020157	23.016951294520233
60-61	22.584056595702158	26.047633758092818	28.906318882454325	22.461990763750695
62-63	22.124842397510626	25.935777536636763	29.32172068446749	22.617659381385124
64-65	22.34049779038126	25.808683231813546	29.328774222453568	22.52204475535163
66-67	22.393652812072876	25.890944608239085	29.2072595178184	22.50814306186964
68-69	22.889335071256653	26.003914409399155	29.000378966556077	22.106371552788115
70-71	22.78862136416925	25.859047164974548	28.910254944925757	22.44207652593045
72-73	22.57149957335864	25.964727062189713	28.80003892510542	22.66373443934623
74-75	22.584831968859294	25.95804364792978	28.723169063068415	22.73395532014251
76-77	22.594334092789005	26.021877156276645	28.657200302650214	22.726588448284136
78-79	22.619647083718668	25.915191283811673	28.95146584740139	22.513695785068265
80-81	22.193274880966396	26.31843973032384	28.855140091543337	22.63314529716643
82-83	22.1939931788762	26.203640327445292	28.975554591849395	22.62681190182911
84-85	22.068715307462664	25.82349364423246	28.73319295761494	23.37459809068994
86-87	22.316071925399473	26.165453628729406	28.527759019085103	22.99071542678602
88-89	21.88383236231434	26.040926809627045	28.70511425107119	23.37012657698742
90-91	21.89537211775813	26.079298736253133	28.5044002059928	23.520928939995944
92-93	21.690589799143005	26.29025144801774	28.853340413202456	23.1658183396368
94-95	21.701646023678276	25.6111624802413	29.0298810502118	23.657310445868628
96-97	21.66510897017648	26.277180497509317	28.73148316871388	23.326227363600324
98-99	21.262987533045926	26.14355521353739	28.81258683459737	23.780870418819315
100	21.44964641376529	26.04651238735613	28.87389601250084	23.629945186377746
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1586.0
1	1128.5
2	1802.0
3	2618.5
4	1474.0
5	1071.5
6	2587.0
7	7315.5
8	12928.5
9	13099.0
10	7655.0
11	3386.0
12	2787.0
13	2903.5
14	3213.5
15	3620.5
16	4020.5
17	4520.5
18	5051.0
19	5562.0
20	6336.0
21	7259.5
22	8351.5
23	10057.0
24	12350.5
25	14823.0
26	17994.0
27	22761.0
28	28570.0
29	34382.5
30	39733.0
31	44816.0
32	49522.5
33	54141.0
34	59185.5
35	64947.5
36	70640.5
37	77265.0
38	84244.5
39	88577.5
40	94458.5
41	100718.5
42	105281.5
43	113336.0
44	117867.5
45	115729.5
46	114536.5
47	116305.5
48	116740.5
49	117292.5
50	120961.0
51	114727.5
52	101315.0
53	91271.5
54	84329.0
55	79676.5
56	70256.0
57	59866.5
58	57317.5
59	56488.5
60	50396.5
61	41447.0
62	34200.5
63	26348.5
64	18633.5
65	14768.0
66	11994.5
67	9672.5
68	7093.0
69	4427.5
70	3041.5
71	2495.0
72	1882.0
73	1227.0
74	913.0
75	671.0
76	434.5
77	285.0
78	182.0
79	82.0
80	32.5
81	20.0
82	9.0
83	10.0
84	10.0
85	4.5
86	1.5
87	2.0
88	2.5
89	2.5
90	2.5
91	1.5
92	1.5
93	1.5
94	2.5
95	5.0
96	5.0
97	8.0
98	6.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008947543556413447
2	3.265526845406367E-5
3	5.224842952650187E-4
4	0.002155247717968202
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.796580536219101E-5
24-25	4.898290268109551E-5
26-27	5.551395637190824E-4
28-29	9.470027851678465E-4
30-31	0.0016164357884761516
32-33	0.004245184899028277
34-35	0.005339136392239411
36-37	0.003641062432628099
38-39	0.002351179328692584
40-41	0.0033471650165415264
42-43	4.7350139258392325E-4
44-45	4.571737583568914E-4
46-47	0.003608407164174035
48-49	0.0025471109394169665
50-51	9.306751509408147E-4
52-53	0.0
54-55	4.2451848990282775E-4
56-57	7.184159059894007E-4
58-59	6.367777348542416E-4
60-61	3.5920795299470036E-4
62-63	0.0
64-65	0.0
66-67	7.673988086704962E-4
68-69	5.714671979461142E-4
70-71	0.002057281912606011
72-73	6.531053690812734E-5
74-75	0.002057281912606011
76-77	3.9186322144876406E-4
78-79	0.0012082449328003559
80-81	8.98019882486751E-4
82-83	0.0013388660066166107
84-85	0.0011429343958922285
86-87	2.93897416086573E-4
88-89	0.0015021423488869288
90-91	0.0016327634227031836
92-93	0.0029226465266386985
94-95	0.0011266067616651967
96-97	0.00257976620787103
98-99	0.0025307833051899345
100	0.0032002163084982396
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3062293.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.71850594740904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.53830559457775	32.438046501460995
2	14.930984599219288	13.35382647201721
3	5.213240786027674	6.9938501728576155
4	2.4290196387569	4.344885166484952
5	1.3059245619481372	2.919949764517266
6	0.8000765003330803	2.1466935443116237
7	0.5132826377431127	1.606726288203203
8	0.3682695255981727	1.3174770376569116
9	0.25919857440097904	1.0431875691819115
>10	1.3692323073747366	11.284546877286296
>50	0.140880258011329	4.45246062810839
>100	0.1174642330099819	10.393192647835647
>500	0.010608878418924059	3.1635180413296835
>1k	0.0032924105437469003	2.4314821259310575
>5k	0.0	0.0
>10k+	2.1949403624979335E-4	2.110157162817207
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28624	0.9347244042291185	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20556	0.6712616983417328	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15315	0.5001154363739851	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3641	0.11889783244124583	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3584	0.1170364821393642	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3381	0.11040746264318928	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.796580536219101E-5	0.0
10-11	0.0	0.0	0.0	9.796580536219101E-5	0.0
12-13	0.0	0.0	0.0	9.796580536219101E-5	0.0
14-15	0.0	0.0	0.0	9.796580536219101E-5	0.0
16-17	0.0	0.0	0.0	1.3062107381625469E-4	0.0
18-19	0.0	0.0	0.0	3.265526845406367E-4	0.0
20-21	0.0	0.0	0.0	5.551395637190824E-4	0.0
22-23	0.0	0.0	0.0	0.0013062107381625469	0.0
24-25	0.0	1.6327634227031836E-5	0.0	0.0024328174998277433	0.0
26-27	0.0	3.265526845406367E-5	0.0	0.00355942426149294	0.0
28-29	0.0	3.265526845406367E-5	0.0	0.007804609160521217	0.0
30-31	0.0	3.265526845406367E-5	0.0	0.016458255300848088	0.0
32-33	0.0	3.265526845406367E-5	0.0	0.028344773018127264	0.0
34-35	0.0	3.265526845406367E-5	0.0	0.0460602561544568	0.0
36-37	0.0	4.898290268109551E-5	0.0	0.07089458781377223	0.0
38-39	0.0	6.531053690812734E-5	0.0	0.10884000975739422	0.0
40-41	0.0	6.531053690812734E-5	0.0	0.1544430921534941	0.0
42-43	0.0	6.531053690812734E-5	0.0	0.19749906361017708	0.0
44-45	0.0	6.531053690812734E-5	0.0	0.24542067006651552	0.0
46-47	0.0	6.531053690812734E-5	0.0	0.30085298826728857	0.0
48-49	0.0	6.531053690812734E-5	0.0	0.36273472198773926	0.0
50-51	0.0	6.531053690812734E-5	0.0	0.43367829270419256	0.0
52-53	0.0	6.531053690812734E-5	0.0	0.5042300001991972	0.0
54-55	0.0	6.531053690812734E-5	0.0	0.5761042460665913	0.0
56-57	0.0	6.531053690812734E-5	0.0	0.6593751806244537	0.0
58-59	0.0	6.531053690812734E-5	0.0	0.7538468722620599	0.0
60-61	0.0	6.531053690812734E-5	0.0	0.8615929305262429	0.0
62-63	0.0	6.531053690812734E-5	0.0	0.973910726373995	0.0
64-65	0.0	6.531053690812734E-5	0.0	1.0850855878258545	0.0
66-67	0.0	6.531053690812734E-5	0.0	1.2097307475150156	0.0
68-69	0.0	6.531053690812734E-5	0.0	1.3541649998873395	0.0
70-71	0.0	6.531053690812734E-5	0.0	1.5226172022076268	0.0
72-73	0.0	6.531053690812734E-5	0.0	1.7039029250303612	3.265526845406367E-5
74-75	0.0	6.531053690812734E-5	0.0	1.889433832752124	3.265526845406367E-5
76-77	0.0	6.531053690812734E-5	0.0	2.080907999332526	3.265526845406367E-5
78-79	0.0	6.531053690812734E-5	0.0	2.3004167138807423	3.265526845406367E-5
80-81	0.0	6.531053690812734E-5	0.0	2.548988617353075	3.265526845406367E-5
82-83	0.0	6.531053690812734E-5	0.0	2.820566157451296	3.265526845406367E-5
84-85	0.0	6.531053690812734E-5	0.0	3.1005034462737564	3.265526845406367E-5
86-87	0.0	6.531053690812734E-5	0.0	3.392735443669172	3.265526845406367E-5
88	0.0	6.531053690812734E-5	0.0	3.6241796588373485	3.265526845406367E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9465	0.0	64.459335	1
GTATCAA	21035	0.0	50.21024	1
ATCAACG	28700	0.0	36.600914	3
TCAACGC	29100	0.0	36.097805	4
TATCAAC	30115	0.0	35.006016	2
CAACGCA	30610	0.0	34.31541	5
AACGCAG	31370	0.0	33.48405	6
ACGCAGA	34135	0.0	30.606562	7
CGCAGAG	34305	0.0	30.235693	8
GCAGAGT	36290	0.0	28.232187	9
GTGGTAT	2910	0.0	27.943762	1
TGGTATC	3115	0.0	26.253517	2
GTACATG	19275	0.0	24.946692	1
TACATGG	18800	0.0	24.84991	2
ACATGGG	19340	0.0	23.208292	3
AGAGTAC	35935	0.0	23.207767	10-11
AGTACTT	25970	0.0	23.17302	12-13
GAGTACT	25955	0.0	22.271994	12-13
CAGAGTA	36450	0.0	21.88705	10-11
GTACTTT	26820	0.0	21.378443	14-15
>>END_MODULE