FastQCFastQC Report
Wed 25 May 2016
SRR2431362_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431362_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3452287
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA737202.135396043260598No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60410.17498545167305035No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG44680.1294214530831301No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT39090.11322928829497662No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC36780.1065380717188345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56050.046.4548841
GTATCAA100300.039.0806961
TCAACGC119550.031.37264
ATCAACG119750.031.359453
CAACGCA133000.028.2352945
AACGCAG136450.027.3147266
TACATGG220750.027.0608862
GTACATG239700.026.7254491
TATCAAC151150.025.4978122
ACGCAGA145350.025.3835187
CGCAGAG148400.024.798488
ACATGGG232350.024.5164263
CATGGGT39650.023.825944
GCAGAGT159750.022.3599039
CATGGGG115200.021.5824324
GTATAAT31500.020.5904451
ATAATAC32500.019.0892013
CATGGGA74900.018.9505734
CTTGGGT25000.018.799974
CAGAGTA153600.018.77243210-11
Produced by FastQC (version 0.11.5)