##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431362_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3452287
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.321724700177015	33.0	31.0	34.0	25.0	34.0
2	30.447976370446604	33.0	31.0	34.0	25.0	34.0
3	30.62060454417608	34.0	31.0	34.0	26.0	34.0
4	33.567898323633	37.0	35.0	37.0	26.0	37.0
5	33.972139917683556	37.0	35.0	37.0	30.0	37.0
6	33.89024927533545	37.0	35.0	37.0	28.0	37.0
7	33.868013290899626	37.0	35.0	37.0	29.0	37.0
8	33.76820090566051	37.0	35.0	37.0	28.0	37.0
9	35.073802091193464	39.0	37.0	39.0	26.0	39.0
10-11	35.17580954306522	39.0	37.0	39.0	27.0	39.0
12-13	35.11816587091398	39.0	37.0	39.0	27.0	39.0
14-15	36.40055505234646	40.5	38.0	41.0	27.0	41.0
16-17	36.42718594948798	40.0	38.0	41.0	27.0	41.0
18-19	36.420290810120946	40.0	38.0	41.0	27.0	41.0
20-21	36.45529905248318	40.0	38.0	41.0	27.0	41.0
22-23	36.49167088367798	40.0	38.0	41.0	27.0	41.0
24-25	36.5394952679195	40.0	37.0	41.0	27.0	41.0
26-27	36.559938962201	40.0	37.0	41.0	28.0	41.0
28-29	36.546335371306036	40.0	37.0	41.0	28.0	41.0
30-31	36.46183109921047	40.0	37.0	41.0	28.0	41.0
32-33	36.368823768128195	40.0	37.0	41.0	27.5	41.0
34-35	36.29516940509291	40.0	37.0	41.0	27.0	41.0
36-37	36.18107431392581	40.0	36.0	41.0	27.0	41.0
38-39	36.05523628249911	40.0	36.0	41.0	27.0	41.0
40-41	35.95589923433364	40.0	36.0	41.0	26.0	41.0
42-43	35.81739163053362	40.0	35.5	41.0	26.0	41.0
44-45	35.68463948680976	40.0	35.0	41.0	25.5	41.0
46-47	35.55106861046025	40.0	35.0	41.0	25.0	41.0
48-49	35.39226648885217	40.0	35.0	41.0	25.5	41.0
50-51	35.242227543654394	40.0	35.0	41.0	26.0	41.0
52-53	35.059829759229174	39.0	35.0	41.0	25.0	41.0
54-55	34.88481621023976	39.0	34.5	41.0	25.5	41.0
56-57	34.65890335884589	39.0	34.0	41.0	25.0	41.0
58-59	34.40490796390914	38.0	34.0	40.5	25.0	41.0
60-61	34.18422222717868	38.0	34.0	40.0	25.0	41.0
62-63	33.97502380306156	37.5	34.0	40.0	24.0	41.0
64-65	33.696046997251386	37.0	33.5	40.0	23.0	41.0
66-67	33.34408321208521	36.5	33.0	40.0	22.5	41.0
68-69	32.993195380337724	36.0	33.0	39.0	21.5	41.0
70-71	32.7137102158656	35.0	33.0	39.0	21.0	41.0
72-73	32.43625341693782	35.0	33.0	39.0	20.0	40.5
74-75	32.10330050775037	35.0	32.5	37.5	18.5	40.0
76-77	31.788060059896527	35.0	32.0	37.0	17.5	39.0
78-79	31.48529757230497	35.0	32.0	36.5	15.0	39.0
80-81	31.18900543320993	35.0	32.0	36.0	11.0	39.0
82-83	30.90317346153434	35.0	32.0	36.0	7.0	37.0
84-85	30.668490626648364	35.0	31.0	35.0	4.0	37.0
86-87	30.45199732814798	35.0	31.0	35.0	2.0	36.5
88-89	30.246701968868752	35.0	31.0	35.0	2.0	36.0
90-91	30.07436447201522	35.0	31.0	35.0	2.0	36.0
92-93	29.911340221713896	35.0	31.0	35.0	2.0	36.0
94-95	29.71308410917169	35.0	31.0	35.0	2.0	35.5
96-97	29.5438493381344	35.0	30.0	35.0	2.0	35.0
98-99	29.43156811701924	35.0	30.5	35.0	2.0	35.0
100	29.391257157936174	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3102476876654663
1101	2	0.3551105524836089
1101	3	0.38091799962554873
1101	4	0.38282081077466046
1101	5	0.2132288097200714
1101	6	0.3156655494331204
1101	7	0.19291824471756058
1101	8	0.29370832603650143
1101	9	0.4372024923303073
1101	10-11	0.30420266582085986
1101	12-13	0.38747608909785924
1101	14-15	0.4464808428108853
1101	16-17	0.3910774379493702
1101	18-19	0.3538868515331117
1101	20-21	0.3170022597133766
1101	22-23	0.29968909259358156
1101	24-25	0.33005822824657116
1101	26-27	0.38919607240598
1101	28-29	0.3589893488387972
1101	30-31	0.41332951340886837
1101	32-33	0.34456310134000745
1101	34-35	0.4233402691557373
1101	36-37	0.3955061634695909
1101	38-39	0.3936462812028765
1101	40-41	0.4292662627907333
1101	42-43	0.48380478324553167
1101	44-45	0.39171686692530727
1101	46-47	0.4113928997455645
1101	48-49	0.4618062655383497
1101	50-51	0.36723247377629775
1101	52-53	0.38013910521449645
1101	54-55	0.395884947348776
1101	56-57	0.3115483111714852
1101	58-59	0.40479547665103865
1101	60-61	0.4315990619250769
1101	62-63	0.4792319927421431
1101	64-65	0.4951671000993443
1101	66-67	0.4040128813719974
1101	68-69	0.4414596692940691
1101	70-71	0.4037832335538596
1101	72-73	0.42974625574900216
1101	74-75	0.33588617519271935
1101	76-77	0.3536025201291899
1101	78-79	0.3017053984267122
1101	80-81	0.31979403617887314
1101	82-83	0.3319032633533787
1101	84-85	0.31361900197474313
1101	86-87	0.2979136293637126
1101	88-89	0.3640014041719155
1101	90-91	0.3079061076715739
1101	92-93	0.340299332556004
1101	94-95	0.35816089133739126
1101	96-97	0.4169892684178045
1101	98-99	0.44991706968357903
1101	100	0.49145762943114946
1102	1	0.25708688446560757
1102	2	0.3266476806100087
1102	3	0.37158110610679174
1102	4	0.27883809844588825
1102	5	0.34683657306304383
1102	6	0.3430806473816901
1102	7	0.332663232242858
1102	8	0.3576958984493217
1102	9	0.44583080885243476
1102	10-11	0.4632786718407331
1102	12-13	0.49661984044829666
1102	14-15	0.45370944828741244
1102	16-17	0.49979518378014376
1102	18-19	0.42600845948901167
1102	20-21	0.3943536310497109
1102	22-23	0.37108506537607866
1102	24-25	0.4003391349480907
1102	26-27	0.4717802211614952
1102	28-29	0.503406780465653
1102	30-31	0.48697154359354045
1102	32-33	0.5483504270035127
1102	34-35	0.5634850336507284
1102	36-37	0.568736309226793
1102	38-39	0.5343987058367361
1102	40-41	0.5810938690086616
1102	42-43	0.59027873754723
1102	44-45	0.6034180109176077
1102	46-47	0.5702892818815641
1102	48-49	0.48486954042953556
1102	50-51	0.5147636211947741
1102	52-53	0.5667561575256386
1102	54-55	0.5719875147882192
1102	56-57	0.5589514282091415
1102	58-59	0.566702755242531
1102	60-61	0.6056409032812908
1102	62-63	0.5827086030746429
1102	64-65	0.6514427221007537
1102	66-67	0.6319425719281213
1102	68-69	0.5924465510858639
1102	70-71	0.5427193471334348
1102	72-73	0.5996048892086279
1102	74-75	0.6238183231523493
1102	76-77	0.5499895256120091
1102	78-79	0.4454610668502994
1102	80-81	0.48431331067880024
1102	82-83	0.49970910128565293
1102	84-85	0.4918082461572837
1102	86-87	0.5385440089419227
1102	88-89	0.47989003984354994
1102	90-91	0.4852176466688327
1102	92-93	0.5200468561490901
1102	94-95	0.47505835356806614
1102	96-97	0.4489574673590653
1102	98-99	0.478093864389745
1102	100	0.5587531628138294
1103	1	0.27452330606132236
1103	2	0.2784655703819645
1103	3	0.2877299225399632
1103	4	0.2792996657621174
1103	5	0.20450415266388688
1103	6	0.3190059328117343
1103	7	0.3391474147871918
1103	8	0.3746062394119605
1103	9	0.38825611145065864
1103	10-11	0.43996495975169836
1103	12-13	0.3648496930773888
1103	14-15	0.45054782888941247
1103	16-17	0.39730283486852613
1103	18-19	0.41048305391139905
1103	20-21	0.4256432263631922
1103	22-23	0.3702351024266761
1103	24-25	0.3636484592827216
1103	26-27	0.40385319489051597
1103	28-29	0.44034444027138875
1103	30-31	0.45920583966221784
1103	32-33	0.331891102034362
1103	34-35	0.3522115607244345
1103	36-37	0.3832265477040977
1103	38-39	0.3118947377372052
1103	40-41	0.2862692843248169
1103	42-43	0.31431031308084556
1103	44-45	0.3309454866936079
1103	46-47	0.2634704831933661
1103	48-49	0.34308206656432816
1103	50-51	0.2360032343915961
1103	52-53	0.24710631798340899
1103	54-55	0.2586078178845099
1103	56-57	0.17500727755729883
1103	58-59	0.14381883341687995
1103	60-61	0.1623326383232353
1103	62-63	0.23952241683581832
1103	64-65	0.2530229078880737
1103	66-67	0.2728847515707997
1103	68-69	0.21575083234660752
1103	70-71	0.12573037207729953
1103	72-73	0.13937987439921073
1103	74-75	0.11302709151376433
1103	76-77	0.09238101389475517
1103	78-79	0.1318093529415627
1103	80-81	0.13877714198801883
1103	82-83	0.1587733687303583
1103	84-85	0.05668975577908597
1103	86-87	0.10480336597249718
1103	88-89	0.07243331055920876
1103	90-91	0.10243666687538067
1103	92-93	0.06267712859009933
1103	94-95	0.09361874144929061
1103	96-97	0.11305521657857298
1103	98-99	0.11338143058964079
1103	100	0.15952616816711895
1104	1	0.3349314998258883
1104	2	0.33694563901387653
1104	3	0.3001704994663612
1104	4	0.30872896824367047
1104	5	0.2910632941077935
1104	6	0.38384718815129304
1104	7	0.27944759017819365
1104	8	0.3745836806814964
1104	9	0.4189314814784737
1104	10-11	0.5315750259659495
1104	12-13	0.4577247786733878
1104	14-15	0.4094344175198259
1104	16-17	0.46641317358056966
1104	18-19	0.42696879483392536
1104	20-21	0.41551220892608143
1104	22-23	0.4168736931339936
1104	24-25	0.4095704037402115
1104	26-27	0.4149112468853531
1104	28-29	0.41106837870382407
1104	30-31	0.5246992549731146
1104	32-33	0.488377220742656
1104	34-35	0.4964329229998867
1104	36-37	0.5339412800061893
1104	38-39	0.5370023253682845
1104	40-41	0.48479547367654163
1104	42-43	0.44083076525954823
1104	44-45	0.4509378550875951
1104	46-47	0.4019587171683483
1104	48-49	0.4730725810560017
1104	50-51	0.3745544952077964
1104	52-53	0.36915647208748226
1104	54-55	0.35522927896802514
1104	56-57	0.42565226543688084
1104	58-59	0.3886013451551307
1104	60-61	0.3881287467629235
1104	62-63	0.411631957527554
1104	64-65	0.3955201952111551
1104	66-67	0.4474715619439493
1104	68-69	0.42594034402973335
1104	70-71	0.3819950482827963
1104	72-73	0.3933039051014404
1104	74-75	0.4624134356675782
1104	76-77	0.5143243872280756
1104	78-79	0.47786918086306684
1104	80-81	0.45143824822646295
1104	82-83	0.39693541430433044
1104	84-85	0.4128605442997042
1104	86-87	0.47218840797468076
1104	88-89	0.49999737297541813
1104	90-91	0.5283061141231009
1104	92-93	0.503124540353145
1104	94-95	0.4665162239867051
1104	96-97	0.5311244302662956
1104	98-99	0.5003183244384282
1104	100	0.47700948450565406
1105	1	0.24306187703942328
1105	2	0.30888822929335547
1105	3	0.37389861797059964
1105	4	0.36051108292557643
1105	5	0.36575784854647964
1105	6	0.29031415965024365
1105	7	0.23192766419344935
1105	8	0.32570677786002733
1105	9	0.3078982041338918
1105	10-11	0.2525133444522467
1105	12-13	0.32031026172795407
1105	14-15	0.4073220747891497
1105	16-17	0.3532207368273603
1105	18-19	0.3555686002994065
1105	20-21	0.35926053593667007
1105	22-23	0.30709446661703055
1105	24-25	0.32862889955776353
1105	26-27	0.3530705523154438
1105	28-29	0.3648686331437361
1105	30-31	0.27001170391029916
1105	32-33	0.3278423747067407
1105	34-35	0.35985451616561903
1105	36-37	0.39225835847251744
1105	38-39	0.3893162251235509
1105	40-41	0.3976548550860244
1105	42-43	0.39961953215065904
1105	44-45	0.33464994615576416
1105	46-47	0.2848620771506063
1105	48-49	0.2579149996937318
1105	50-51	0.28474264420191986
1105	52-53	0.37022951367224977
1105	54-55	0.3805451688894621
1105	56-57	0.3153766823467947
1105	58-59	0.38406127078563657
1105	60-61	0.4177012331584393
1105	62-63	0.45701692242212033
1105	64-65	0.45994713703682066
1105	66-67	0.43984601487277075
1105	68-69	0.3621450739444896
1105	70-71	0.4718272724433703
1105	72-73	0.40873964654996797
1105	74-75	0.4194035648325567
1105	76-77	0.3619018819270998
1105	78-79	0.3631149962794602
1105	80-81	0.33597416314617234
1105	82-83	0.3383347165778332
1105	84-85	0.33893883924793045
1105	86-87	0.3203411532489646
1105	88-89	0.3729602134222709
1105	90-91	0.4018253080047707
1105	92-93	0.47354978796389346
1105	94-95	0.48538692089578106
1105	96-97	0.4403286598329963
1105	98-99	0.40552374030482596
1105	100	0.40772778622608996
1106	1	0.20228602432900544
1106	2	0.25809574149624837
1106	3	0.21244824112698524
1106	4	0.16387170393183936
1106	5	0.2112921516879993
1106	6	0.15921787713019597
1106	7	0.21153575207193853
1106	8	0.28222780539597636
1106	9	0.2354023908251861
1106	10-11	0.2436513107710141
1106	12-13	0.28727819456282333
1106	14-15	0.2684593244401796
1106	16-17	0.3012871838263109
1106	18-19	0.27537527099343606
1106	20-21	0.31672466894989526
1106	22-23	0.32751759094717414
1106	24-25	0.3792068876607786
1106	26-27	0.41368953296591826
1106	28-29	0.41196648886693055
1106	30-31	0.45761970229195725
1106	32-33	0.4288936619687007
1106	34-35	0.42519071863453206
1106	36-37	0.4350222035901794
1106	38-39	0.4631979298130773
1106	40-41	0.5099356441969007
1106	42-43	0.5147035665456272
1106	44-45	0.48720888707103427
1106	46-47	0.48553075780380794
1106	48-49	0.4623870256410285
1106	50-51	0.438151516086414
1106	52-53	0.42918900992992803
1106	54-55	0.4750329773160473
1106	56-57	0.4206092502705161
1106	58-59	0.4073251297090721
1106	60-61	0.35644562742843533
1106	62-63	0.5025388408057125
1106	64-65	0.5728749103686397
1106	66-67	0.5296888073922972
1106	68-69	0.5292835436636167
1106	70-71	0.5438362253602307
1106	72-73	0.537150889676596
1106	74-75	0.5761432665270192
1106	76-77	0.5330284092891127
1106	78-79	0.5796204165557945
1106	80-81	0.5710583569717933
1106	82-83	0.646268802246702
1106	84-85	0.587049191291996
1106	86-87	0.6054316376535844
1106	88-89	0.5682927616944262
1106	90-91	0.5078635013504815
1106	92-93	0.5435813964385652
1106	94-95	0.5753005522281107
1106	96-97	0.5520933018343541
1106	98-99	0.5751530636530227
1106	100	0.6361006042549064
1107	1	-0.04429599009998242
1107	2	0.03457741005856718
1107	3	0.04668749409530548
1107	4	-0.005606955766893407
1107	5	0.053020934690486854
1107	6	-0.03378256595108553
1107	7	0.05348155947834243
1107	8	-0.02584665074415682
1107	9	-0.02758893015919739
1107	10-11	0.057066251208517826
1107	12-13	-0.0219880928053513
1107	14-15	0.07921726510369353
1107	16-17	0.11120990087243143
1107	18-19	0.07028551132441407
1107	20-21	0.006727328977994773
1107	22-23	0.018335379092143
1107	24-25	0.048416848811335456
1107	26-27	0.11289885554168677
1107	28-29	0.055647007098386325
1107	30-31	0.0692388296795059
1107	32-33	0.11807326420942132
1107	34-35	0.09464051293223008
1107	36-37	0.11555471895029257
1107	38-39	0.11493401301377304
1107	40-41	0.1291742221804668
1107	42-43	0.06221773212302395
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2216	64-65	-0.8397226841019929
2216	66-67	-0.8570908691873385
2216	68-69	-0.8686860292275327
2216	70-71	-0.9643279044555371
2216	72-73	-0.8793126381300986
2216	74-75	-0.9172292573759719
2216	76-77	-0.8461471631239093
2216	78-79	-0.826386339150968
2216	80-81	-0.7607435126799409
2216	82-83	-0.6876727722313625
2216	84-85	-0.7505335994911633
2216	86-87	-0.7290978859810444
2216	88-89	-0.7949313296627132
2216	90-91	-0.8231363338872377
2216	92-93	-0.8624166605444152
2216	94-95	-0.9313482629256704
2216	96-97	-1.0679069971842736
2216	98-99	-1.1079166374385885
2216	100	-1.042054680348155
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	85379.0
3	21657.0
4	18281.0
5	17894.0
6	10243.0
7	8215.0
8	9089.0
9	8818.0
10	8084.0
11	8858.0
12	10476.0
13	12147.0
14	12607.0
15	12568.0
16	12580.0
17	11726.0
18	10948.0
19	9976.0
20	9178.0
21	9600.0
22	12687.0
23	25982.0
24	47571.0
25	52936.0
26	49312.0
27	32374.0
28	32429.0
29	35190.0
30	40165.0
31	47416.0
32	62365.0
33	82420.0
34	118106.0
35	209410.0
36	360007.0
37	696432.0
38	901618.0
39	334154.0
40	3389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.32878656652	23.111923459057373	12.971465151914538	21.58782482250809
2	15.247653581022638	24.27437851253171	38.11334068306123	22.364627223384417
3	16.745541939456928	32.06828750572809	28.862498645824825	22.323671908990157
4	11.502649116541141	21.562455916852702	37.86560951115538	29.069285455450782
5	11.719661534219085	43.12852013333781	32.88860360271635	12.263214729726755
6	26.237998173384774	42.58400301017847	18.14704281538586	13.030956001050898
7	24.28207735915351	37.87781838531964	22.185727895739838	15.654376359787006
8	23.72189797661666	38.828144936964975	19.85367960427392	17.596277482144444
9	25.120072577975122	20.962828409109672	20.09201436612889	33.825084646786316
10-11	22.590213386082908	31.393595028455053	26.91240618175719	19.103785403704848
12-13	23.124134812661868	30.500665211206368	26.141670724363298	20.233529251768463
14-15	21.342359427243448	30.47657972816281	25.053928019310096	23.127132825283645
16-17	20.778182694544224	33.281865036134015	24.989477989518253	20.950474279803505
18-19	20.847643895191794	32.23302407940012	26.956072887335264	19.96325913807282
20-21	22.980186757358236	30.641629737040983	26.323043246404488	20.055140259196293
22-23	22.200631059932157	31.026794701599258	26.344724526089518	20.42784971237907
24-25	21.443350451454354	31.41211898083792	26.0485730184078	21.09595754929993
26-27	21.169531965331966	31.626802754232198	26.165336195976753	21.038329084459086
28-29	21.47099879007742	31.018597237135847	26.727166657928496	20.783237314858237
30-31	21.774131177390522	31.16578951865821	26.266385152798712	20.793694151152557
32-33	20.81825757823727	31.060902526354266	26.876632794434528	21.244207100973934
34-35	21.684031484056803	30.79383318941907	26.572472103275306	20.94966322324882
36-37	22.21375279633472	30.83167766758673	25.910997550319543	21.043571985759005
38-39	21.5777106596294	30.811010208595057	26.350083292611536	21.26119583916401
40-41	21.30774469214176	31.012311548837047	26.558843456526066	21.12110030249513
42-43	21.319476045879153	30.929120319370902	26.74984727515412	21.001556359595828
44-45	21.12725564241907	30.743967694458775	26.453304722347827	21.675471940774333
46-47	21.752855425982833	30.83889027766232	26.3299372271193	21.078317069235553
48-49	21.411545447988537	30.512932441595964	26.68858353896996	21.386938571445537
50-51	21.592613823821715	30.869739393045826	25.93679204538904	21.600854737743415
52-53	21.28888762724536	30.973033238545927	26.279101360923935	21.458977773284783
54-55	21.320518832878033	31.109522470177016	26.279666203881657	21.290292493063294
56-57	21.49451943016325	31.051430544447783	26.042113532275852	21.411936493113114
58-59	21.772900109405736	30.25713099750977	26.183280822249134	21.78668807083536
60-61	21.881567204580616	30.696129840885188	26.15286620144849	21.269436753085706
62-63	20.988064140688227	30.888981967310936	26.574666757041566	21.54828713495927
64-65	21.409625390248664	30.782081785808014	26.37114943547551	21.437143388467817
66-67	21.39761265503129	30.535468227293965	26.12418955897931	21.94272955869544
68-69	21.65580381932325	30.902616149816048	26.120235658275227	21.321344372585475
70-71	21.906376845262283	30.710511611578063	26.152446190018384	21.230665353141266
72-73	21.543298688666383	30.5444622651593	26.3254908992213	21.58674814695302
74-75	21.42224849787981	30.499998986179307	26.505835117416364	21.571917398524516
76-77	21.485308145006485	30.96626960620597	25.9736806354744	21.574741613313144
78-79	21.56403856342187	31.051618825433692	26.12022117512246	21.264121436021977
80-81	21.411009571336333	31.22882019947936	26.149955087743283	21.210215141441022
82-83	21.231900388264695	31.24340291818402	26.28496091283336	21.239735780717925
84-85	21.480857951683372	30.53672816341948	26.110639118794044	21.871774766103105
86-87	21.68844833916407	31.029566927679824	25.9550684661681	21.326916266988004
88-89	21.330102721914194	30.81838470684287	26.21774705262711	21.63376551861582
90-91	21.276762767012123	30.889800222031845	25.966544595016376	21.866892415939656
92-93	21.19558070135698	30.99806443502185	26.513764178549355	21.29259068507182
94-95	21.561066819997844	30.449746503882647	26.31840792417059	21.670778751948927
96-97	21.391544621104888	31.33893078050888	25.73940282342261	21.53012177496362
98-99	20.659816641641573	31.06591547451677	26.3387145854609	21.935553298380757
100	20.942261267143387	31.171807244411255	26.135027863343037	21.750903625102325
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	67807.0
1	45911.0
2	18540.0
3	9993.5
4	5463.0
5	3395.5
6	2695.0
7	2834.5
8	3380.5
9	3517.5
10	2597.5
11	1691.5
12	1533.5
13	1549.5
14	1594.0
15	1662.0
16	1829.0
17	2141.0
18	2533.5
19	3087.0
20	4027.5
21	5240.5
22	6773.0
23	9139.0
24	12332.0
25	16008.0
26	20615.0
27	26508.0
28	33568.0
29	40415.0
30	45430.0
31	50401.5
32	56908.5
33	62258.5
34	67166.0
35	77019.0
36	88029.0
37	97347.0
38	111104.5
39	120818.5
40	127801.5
41	134189.5
42	134104.5
43	137266.0
44	138331.0
45	131855.5
46	128891.5
47	126090.0
48	116069.0
49	114041.0
50	116646.5
51	109670.0
52	98737.5
53	88692.5
54	82157.5
55	78797.0
56	71314.5
57	61477.5
58	56046.5
59	52676.5
60	48015.0
61	41363.0
62	34525.5
63	27393.5
64	21088.5
65	16934.5
66	13574.5
67	10394.0
68	7814.5
69	5821.0
70	4317.5
71	3387.0
72	2744.0
73	2050.5
74	1582.5
75	1299.0
76	953.5
77	746.0
78	559.0
79	372.5
80	275.5
81	197.0
82	140.0
83	112.0
84	95.5
85	89.0
86	91.0
87	77.5
88	59.5
89	56.0
90	48.0
91	40.0
92	34.0
93	32.5
94	27.0
95	19.0
96	20.0
97	17.5
98	12.0
99	16.5
100	43.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.92427193915222E-4
2	1.448315276221241E-4
3	2.8966305524424824E-5
4	1.158652220976993E-4
5	8.689891657327448E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	2.8966305524424824E-5
64-65	2.8966305524424824E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	8.689891657327448E-5
84-85	5.213934994396469E-4
86-87	9.993375405926565E-4
88-89	0.002969046316253544
90-91	0.0023028212891917734
92-93	0.0018828098590876136
94-95	0.0018683267063254012
96-97	0.001694528873178852
98-99	0.0024766191223383224
100	0.0023462707474784105
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3452287.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.43446555506815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.06609465020867	34.856539863886
2	13.65164993064889	12.67814136949129
3	4.775733185764706	6.6527585434376135
4	2.155885794398425	4.004296186426176
5	1.1688979751158144	2.713857638145209
6	0.7124482089968511	1.9849291092260568
7	0.464907169716863	1.5111401170965328
8	0.31579206376389213	1.1730908565926668
9	0.2464006907304733	1.0297335947822246
>10	1.21918017114766	10.446291801154315
>50	0.11696994954436646	3.7996234214830062
>100	0.08832937518405252	8.37267290786262
>500	0.011264841910065847	3.648955489631561
>1k	0.006320827959221372	4.810128117411476
>5k	6.258245504179577E-5	0.17555042414911784
>10k+	6.258245504179577E-5	2.1422905592241297
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	73720	2.135396043260598	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6041	0.17498545167305035	No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG	4468	0.1294214530831301	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT	3909	0.11322928829497662	No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC	3678	0.1065380717188345	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.8966305524424824E-5	0.0
5	0.0	0.0	0.0	2.8966305524424824E-5	0.0
6	0.0	0.0	0.0	2.8966305524424824E-5	0.0
7	0.0	0.0	0.0	5.793261104884965E-5	0.0
8	0.0	0.0	0.0	8.689891657327447E-5	0.0
9	0.0	0.0	0.0	8.689891657327447E-5	0.0
10-11	0.0	0.0	0.0	1.303483748599117E-4	0.0
12-13	0.0	0.0	0.0	1.4483152762212413E-4	0.0
14-15	0.0	0.0	0.0	1.4483152762212413E-4	0.0
16-17	0.0	0.0	0.0	1.7379783314654893E-4	0.0
18-19	0.0	0.0	0.0	2.317304441953986E-4	0.0
20-21	0.0	0.0	0.0	4.2001143010415994E-4	0.0
22-23	0.0	0.0	0.0	9.703712350682316E-4	0.0
24-25	0.0	0.0	0.0	0.0017669446369899143	0.0
26-27	0.0	1.4483152762212412E-5	0.0	0.003026978927302394	0.0
28-29	0.0	2.8966305524424824E-5	0.0	0.006473969284708948	0.0
30-31	0.0	2.8966305524424824E-5	0.0	0.013657613054766305	0.0
32-33	0.0	2.8966305524424824E-5	0.0	0.02437514609880349	0.0
34-35	0.0	2.8966305524424824E-5	0.0	0.038785883097204835	0.0
36-37	0.0	2.8966305524424824E-5	0.0	0.0597430051441262	0.0
38-39	0.0	2.8966305524424824E-5	0.0	0.09185215481795111	0.0
40-41	0.0	2.8966305524424824E-5	0.0	0.12849453130634853	0.0
42-43	0.0	2.8966305524424824E-5	0.0	0.16251545714478546	0.0
44-45	0.0	2.8966305524424824E-5	0.0	0.20146065492237464	0.0
46-47	0.0	2.8966305524424824E-5	0.0	0.2478357100669788	0.0
48-49	0.0	2.8966305524424824E-5	0.0	0.29826604798500245	0.0
50-51	0.0	2.8966305524424824E-5	0.0	0.35551795085402804	0.0
52-53	0.0	2.8966305524424824E-5	0.0	0.4124657075150473	0.0
54-55	0.0	2.8966305524424824E-5	0.0	0.4719480159094537	0.0
56-57	0.0	2.8966305524424824E-5	0.0	0.5414381828625487	0.0
58-59	0.0	2.8966305524424824E-5	0.0	0.6200961855141244	0.0
60-61	0.0	2.8966305524424824E-5	0.0	0.7112386658467271	0.0
62-63	2.8966305524424824E-5	2.8966305524424824E-5	0.0	0.8050894957458636	0.0
64-65	2.8966305524424824E-5	4.344945828663723E-5	0.0	0.9009100344206609	0.0
66-67	2.8966305524424824E-5	5.793261104884965E-5	0.0	1.0026541825752031	0.0
68-69	2.8966305524424824E-5	5.793261104884965E-5	0.0	1.1309604328956429	0.0
70-71	2.8966305524424824E-5	5.793261104884965E-5	0.0	1.276545084461402	0.0
72-73	2.8966305524424824E-5	5.793261104884965E-5	0.0	1.4313555043366906	0.0
74-75	2.8966305524424824E-5	5.793261104884965E-5	0.0	1.5867597334752297	0.0
76-77	2.8966305524424824E-5	5.793261104884965E-5	0.0	1.7523601021583664	0.0
78-79	2.8966305524424824E-5	5.793261104884965E-5	0.0	1.936571322140946	0.0
80-81	2.8966305524424824E-5	5.793261104884965E-5	0.0	2.1544703554484315	0.0
82-83	2.8966305524424824E-5	5.793261104884965E-5	0.0	2.3902271161117254	0.0
84-85	2.8966305524424824E-5	5.793261104884965E-5	0.0	2.633775812961089	0.0
86-87	2.8966305524424824E-5	5.793261104884965E-5	0.0	2.8886068858122167	0.0
88	2.8966305524424824E-5	5.793261104884965E-5	0.0	3.08861922545837	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5605	0.0	46.454884	1
GTATCAA	10030	0.0	39.080696	1
TCAACGC	11955	0.0	31.3726	4
ATCAACG	11975	0.0	31.35945	3
CAACGCA	13300	0.0	28.235294	5
AACGCAG	13645	0.0	27.314726	6
TACATGG	22075	0.0	27.060886	2
GTACATG	23970	0.0	26.725449	1
TATCAAC	15115	0.0	25.497812	2
ACGCAGA	14535	0.0	25.383518	7
CGCAGAG	14840	0.0	24.79848	8
ACATGGG	23235	0.0	24.516426	3
CATGGGT	3965	0.0	23.82594	4
GCAGAGT	15975	0.0	22.359903	9
CATGGGG	11520	0.0	21.582432	4
GTATAAT	3150	0.0	20.590445	1
ATAATAC	3250	0.0	19.089201	3
CATGGGA	7490	0.0	18.950573	4
CTTGGGT	2500	0.0	18.79997	4
CAGAGTA	15360	0.0	18.772432	10-11
>>END_MODULE