FastQCFastQC Report
Wed 25 May 2016
SRR2431362_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431362_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3452287
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT512341.4840596972383813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337740.978308002781924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292930.8485099877269763No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154650.4479639149352299No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82840.23995687496433526No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73270.21223612057746066No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65090.18854168265848117No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48400.14019691873821616No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44000.12745174430746922No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG43600.12629309208649223No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT41770.12099225817552249No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG40190.11641558190266336No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC35710.10343867702772105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112400.066.575181
GTATCAA251300.051.100341
ATCAACG349050.036.6380583
TCAACGC352850.036.2562874
CAACGCA365200.035.017335
TATCAAC370050.034.685892
AACGCAG372550.034.301256
ACGCAGA412850.030.8618897
CGCAGAG417850.030.4363578
TAACGCA2450.028.7746754
GCAGAGT438100.028.772059
GTGGTAT31100.028.4140451
TGGTATC34400.025.549052
TATAACG2601.8189894E-1225.3073272
TACATGG206350.024.8491672
GTACATG222950.024.2662931
AGAGTAC431150.023.67645610-11
AGTACTT327050.023.65380312-13
ACATGGG211200.023.0100843
GAGTACT325100.022.77648512-13
Produced by FastQC (version 0.11.5)