##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431362_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3452287
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71861319757019	34.0	31.0	34.0	31.0	34.0
2	32.829129791352806	34.0	31.0	34.0	31.0	34.0
3	32.90492447470329	34.0	31.0	34.0	31.0	34.0
4	36.305213906028094	37.0	37.0	37.0	35.0	37.0
5	36.27772227511792	37.0	37.0	37.0	35.0	37.0
6	36.31911222908177	37.0	37.0	37.0	35.0	37.0
7	36.18204859561212	37.0	37.0	37.0	35.0	37.0
8	36.236809106543	37.0	37.0	37.0	35.0	37.0
9	38.0354139154711	39.0	39.0	39.0	35.0	39.0
10-11	38.02814815222489	39.0	39.0	39.0	35.0	39.0
12-13	38.0030836080546	39.0	39.0	39.0	35.0	39.0
14-15	39.40810034044099	41.0	39.0	41.0	36.0	41.0
16-17	39.43582138449092	41.0	39.0	41.0	36.5	41.0
18-19	39.54998396714989	41.0	40.0	41.0	37.0	41.0
20-21	39.53520926852258	41.0	40.0	41.0	37.0	41.0
22-23	39.433021501398926	41.0	39.0	41.0	36.0	41.0
24-25	39.382648806428904	41.0	39.0	41.0	36.0	41.0
26-27	39.2576770123689	41.0	39.0	41.0	35.0	41.0
28-29	39.14487367359666	41.0	39.0	41.0	35.0	41.0
30-31	38.86158494354612	41.0	38.5	41.0	35.0	41.0
32-33	38.54122267354945	41.0	38.0	41.0	35.0	41.0
34-35	38.38819802640974	40.5	38.0	41.0	35.0	41.0
36-37	38.23568724153003	40.0	38.0	41.0	35.0	41.0
38-39	38.077629553973935	40.0	38.0	41.0	34.0	41.0
40-41	37.93347728621635	40.0	37.0	41.0	34.0	41.0
42-43	37.71832020338981	40.0	37.0	41.0	33.5	41.0
44-45	37.393699886481045	40.0	37.0	41.0	33.0	41.0
46-47	36.895262329001035	40.0	37.0	41.0	31.5	41.0
48-49	36.4492960753263	40.0	36.0	41.0	30.0	41.0
50-51	36.23719421357494	40.0	36.0	41.0	30.0	41.0
52-53	36.10520040772972	40.0	35.0	41.0	30.0	41.0
54-55	35.932619014583665	40.0	35.0	41.0	29.0	41.0
56-57	35.73022521012882	39.5	35.0	41.0	28.5	41.0
58-59	35.511984374416144	39.0	35.0	41.0	28.0	41.0
60-61	35.281716728649734	39.0	35.0	41.0	28.0	41.0
62-63	35.019814401293985	38.5	35.0	41.0	27.5	41.0
64-65	34.738121569846314	37.5	35.0	40.5	26.5	41.0
66-67	34.41722197488215	37.0	35.0	40.0	26.0	41.0
68-69	34.123616605456036	36.5	35.0	39.5	26.0	41.0
70-71	33.85236887894894	36.0	35.0	39.0	26.0	41.0
72-73	33.57768067950318	35.5	35.0	39.0	26.0	41.0
74-75	33.290918454925674	35.0	35.0	38.0	26.0	40.5
76-77	32.9954834288111	35.0	34.0	37.0	26.0	39.5
78-79	32.70915598847952	35.0	34.0	37.0	25.5	39.0
80-81	32.44083675546095	35.0	34.0	36.0	25.0	39.0
82-83	32.16834072601728	35.0	34.0	36.0	24.5	37.5
84-85	31.955070942827174	35.0	34.0	36.0	24.0	37.0
86-87	31.776055698729564	35.0	34.0	35.0	24.0	37.0
88-89	31.585831218551643	35.0	34.0	35.0	23.0	36.0
90-91	31.41779941818279	35.0	34.0	35.0	21.0	36.0
92-93	31.292733193966782	35.0	34.0	35.0	20.0	36.0
94-95	31.161264981735293	35.0	34.0	35.0	19.5	36.0
96-97	31.028145979751976	35.0	34.0	35.0	16.5	35.5
98-99	30.91436285569537	35.0	33.5	35.0	2.0	35.0
100	30.808145151315635	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13755425727023862
1101	2	0.12781814113267131
1101	3	0.17205242683774458
1101	4	0.08182132910587825
1101	5	0.09583497069003499
1101	6	0.14511945342347587
1101	7	0.15440136455722353
1101	8	0.14937599585698536
1101	9	0.19021337973030228
1101	10-11	0.1873489228242633
1101	12-13	0.1963421578258462
1101	14-15	0.21071327966595987
1101	16-17	0.2552190772096381
1101	18-19	0.24514959946260007
1101	20-21	0.1905839251642476
1101	22-23	0.2766574746284576
1101	24-25	0.2417881916812732
1101	26-27	0.24210840845825032
1101	28-29	0.20951799804159776
1101	30-31	0.2534226596476401
1101	32-33	0.22014674578609572
1101	34-35	0.2504131811743022
1101	36-37	0.2659759755695177
1101	38-39	0.28598630315244833
1101	40-41	0.24368953811273286
1101	42-43	0.2863333700432804
1101	44-45	0.27221856426883306
1101	46-47	0.39805766602206205
1101	48-49	0.3994354540699163
1101	50-51	0.36828424527654846
1101	52-53	0.3047428566180841
1101	54-55	0.3349086790166069
1101	56-57	0.30040341065524245
1101	58-59	0.2718167052156346
1101	60-61	0.2619828587014865
1101	62-63	0.27805020868262886
1101	64-65	0.308804370133835
1101	66-67	0.2678587834697339
1101	68-69	0.2513375248509959
1101	70-71	0.1683542208421258
1101	72-73	0.18751258103798563
1101	74-75	0.18963375020163653
1101	76-77	0.19517530800617067
1101	78-79	0.18003255868221402
1101	80-81	0.19155384225344108
1101	82-83	0.1912749813288812
1101	84-85	0.18656521351898547
1101	86-87	0.14120326021107488
1101	88-89	0.1755600834381461
1101	90-91	0.18177735904405168
1101	92-93	0.263397373797595
1101	94-95	0.25516912081450016
1101	96-97	0.21650812870493397
1101	98-99	0.255311483601794
1101	100	0.31124883261190917
1102	1	0.061233024525819246
1102	2	0.11720115756725846
1102	3	0.11762983158535434
1102	4	0.03845666487534771
1102	5	0.08980009556828605
1102	6	0.06859279576842425
1102	7	0.12037598797552818
1102	8	0.1277255783506277
1102	9	0.12323801849240823
1102	10-11	0.14675035524938806
1102	12-13	0.134027590681562
1102	14-15	0.17947971526202622
1102	16-17	0.19098959077875577
1102	18-19	0.19661015133865334
1102	20-21	0.20354358638116565
1102	22-23	0.21511039718151892
1102	24-25	0.22082623620371322
1102	26-27	0.20084995878231382
1102	28-29	0.2414922250603624
1102	30-31	0.1540807547016101
1102	32-33	0.21078890362920788
1102	34-35	0.22636675249385263
1102	36-37	0.18500354662793228
1102	38-39	0.23839627723513246
1102	40-41	0.2426038428774504
1102	42-43	0.30845701300974326
1102	44-45	0.2555087975605588
1102	46-47	0.25833760310089815
1102	48-49	0.2948812286911249
1102	50-51	0.27211443441880334
1102	52-53	0.21176835435986874
1102	54-55	0.21753884637228538
1102	56-57	0.19592212073899162
1102	58-59	0.26550674027340193
1102	60-61	0.24284537997183264
1102	62-63	0.18406115105412368
1102	64-65	0.28270390610867224
1102	66-67	0.3263757686682851
1102	68-69	0.26441441293678736
1102	70-71	0.25265412718888314
1102	72-73	0.27641928529477156
1102	74-75	0.30105558044395764
1102	76-77	0.24262397336843833
1102	78-79	0.2560425972696123
1102	80-81	0.2134626120459444
1102	82-83	0.1933679426634214
1102	84-85	0.22447619907887528
1102	86-87	0.22489920449487855
1102	88-89	0.22439383855164508
1102	90-91	0.26922742753754747
1102	92-93	0.2628038433013842
1102	94-95	0.31313043269859264
1102	96-97	0.3325503093435884
1102	98-99	0.35276052945972936
1102	100	0.36481873278779986
1103	1	0.0756742880581811
1103	2	0.09973438924684785
1103	3	0.13874864784068563
1103	4	0.05891209748446613
1103	5	0.08810513549715182
1103	6	0.10591786653041169
1103	7	0.055984822004703005
1103	8	0.05098265878264385
1103	9	0.1312437391258996
1103	10-11	0.14558915798918548
1103	12-13	0.14613077701550736
1103	14-15	0.19145845880321133
1103	16-17	0.22549301034531766
1103	18-19	0.23085984682094818
1103	20-21	0.21677673194820812
1103	22-23	0.23298190167917454
1103	24-25	0.21390470005844975
1103	26-27	0.25451444887436026
1103	28-29	0.19715294392461402
1103	30-31	0.21300263446516965
1103	32-33	0.22930959459909417
1103	34-35	0.3516870112414878
1103	36-37	0.381293037743923
1103	38-39	0.39476469445225604
1103	40-41	0.42262547351349866
1103	42-43	0.4131419658345905
1103	44-45	0.34286432752112006
1103	46-47	0.4231895002614081
1103	48-49	0.38034970169105264
1103	50-51	0.42104928348120296
1103	52-53	0.42291630219762766
1103	54-55	0.38938670342876947
1103	56-57	0.43029140353808515
1103	58-59	0.37704495972368335
1103	60-61	0.427037096232759
1103	62-63	0.42068795429152317
1103	64-65	0.4792935114768966
1103	66-67	0.4419688835184985
1103	68-69	0.3730365455798079
1103	70-71	0.3608389911169283
1103	72-73	0.40286273362153935
1103	74-75	0.3377889749602545
1103	76-77	0.31120058931985284
1103	78-79	0.30767956538649344
1103	80-81	0.29364016542679394
1103	82-83	0.2748332370243176
1103	84-85	0.28321829041470536
1103	86-87	0.26336217132565665
1103	88-89	0.2860742143688775
1103	90-91	0.31764407762669933
1103	92-93	0.26707318181536266
1103	94-95	0.3498994580895527
1103	96-97	0.38412295513699846
1103	98-99	0.345967149867235
1103	100	0.4303125247180084
1104	1	0.03930673737004753
1104	2	0.0750076021596442
1104	3	0.09545414916031092
1104	4	0.01131672054809485
1104	5	0.08157969954864086
1104	6	0.10920393550107832
1104	7	0.05281450725912862
1104	8	0.10760882509194403
1104	9	0.13278907471015344
1104	10-11	0.17007957878497848
1104	12-13	0.2108923679288992
1104	14-15	0.2390187675467459
1104	16-17	0.22658450263493535
1104	18-19	0.20928175395853543
1104	20-21	0.2015725645959563
1104	22-23	0.2556647677104209
1104	24-25	0.24769436328954697
1104	26-27	0.17016552093945592
1104	28-29	0.17803454494899285
1104	30-31	0.24714812935326336
1104	32-33	0.18159450210787753
1104	34-35	0.17247793367506148
1104	36-37	0.22171576833772377
1104	38-39	0.16784896828172435
1104	40-41	0.19953605829500276
1104	42-43	0.29676220507722917
1104	44-45	0.24841469674623795
1104	46-47	0.25364043802492375
1104	48-49	0.2898343802266439
1104	50-51	0.25345407417162846
1104	52-53	0.28101135876666916
1104	54-55	0.22885838129710834
1104	56-57	0.21226500557022376
1104	58-59	0.20604015050256663
1104	60-61	0.15644091276965355
1104	62-63	0.13745462855003865
1104	64-65	0.2009546171315577
1104	66-67	0.16601533515529354
1104	68-69	0.10874646549173406
1104	70-71	0.11011713259110678
1104	72-73	0.07251053721430623
1104	74-75	0.14632875542585566
1104	76-77	0.11414567953517718
1104	78-79	0.13243477814200588
1104	80-81	0.1580753719575938
1104	82-83	0.1529629898381799
1104	84-85	0.18107167084255238
1104	86-87	0.16426667217183777
1104	88-89	0.1860762409017802
1104	90-91	0.18807725862535918
1104	92-93	0.21858761068793342
1104	94-95	0.19986663743012656
1104	96-97	0.21716193387116434
1104	98-99	0.1947555417199709
1104	100	0.17425308709172782
1105	1	-0.05156938229072239
1105	2	0.01642305743438044
1105	3	0.03634297917493967
1105	4	-0.046469071779277726
1105	5	-0.10716335913045327
1105	6	-0.14971981809217993
1105	7	-0.5505784628715844
1105	8	-0.42730922368537705
1105	9	-0.3594734090235434
1105	10-11	-0.33711794493706293
1105	12-13	-0.2096197940544826
1105	14-15	-0.1951803084433763
1105	16-17	-0.13175247084062391
1105	18-19	-0.1853528427016471
1105	20-21	-0.16009171924317656
1105	22-23	-0.08468291805304773
1105	24-25	-0.12521272123800742
1105	26-27	-0.14011974563579344
1105	28-29	-0.12854295299310792
1105	30-31	-0.17036961545659324
1105	32-33	-0.17545231016281804
1105	34-35	-0.1173860102400539
1105	36-37	-0.039898260333878
1105	38-39	-0.05239227647977174
1105	40-41	-0.07523060879575638
1105	42-43	-0.08119446978776068
1105	44-45	-0.011290201018361756
1105	46-47	-0.05991228757471134
1105	48-49	-0.04217347150402162
1105	50-51	-0.03272291083265344
1105	52-53	-0.08376529865391547
1105	54-55	-0.049503960498078925
1105	56-57	-0.035552987983372475
1105	58-59	0.013928577793983266
1105	60-61	0.01939664672751462
1105	62-63	-0.010712049467386464
1105	64-65	0.02461932938902578
1105	66-67	0.06994882171137817
1105	68-69	0.10359920874532946
1105	70-71	0.07139861732675712
1105	72-73	0.08833883609164417
1105	74-75	0.03757799902052028
1105	76-77	0.08220890932548741
1105	78-79	0.06284022720416971
1105	80-81	0.043111905181760335
1105	82-83	0.07712115653365714
1105	84-85	0.10256507048093866
1105	86-87	0.10853694693251725
1105	88-89	0.06980448497117564
1105	90-91	0.13814970390036407
1105	92-93	0.17395021583731918
1105	94-95	0.12179350711819126
1105	96-97	0.05436714367765205
1105	98-99	0.03826387296817302
1105	100	0.0014726030106047006
1106	1	0.14816144947555188
1106	2	0.14673169861809754
1106	3	0.12205216618913539
1106	4	0.07015691503966082
1106	5	0.09120495671538009
1106	6	0.10913664129268597
1106	7	0.10890223298390822
1106	8	0.11551171223955947
1106	9	0.1557167314768364
1106	10-11	0.12638934148660752
1106	12-13	0.11805644680796235
1106	14-15	0.14748199013150298
1106	16-17	0.19652554371477038
1106	18-19	0.1995337206562624
1106	20-21	0.15943305187082757
1106	22-23	0.20508175344683366
1106	24-25	0.18028879541346754
1106	26-27	0.172008425209782
1106	28-29	0.1489608659294035
1106	30-31	0.1665505417112243
1106	32-33	0.17122467559506305
1106	34-35	0.1685162226478525
1106	36-37	0.2010913180232663
1106	38-39	0.20750296602868445
1106	40-41	0.21321652625275078
1106	42-43	0.21508958680426105
1106	44-45	0.14313831460948023
1106	46-47	0.20633093281971782
1106	48-49	0.14809655665396804
1106	50-51	0.23164361053261473
1106	52-53	0.17217810469681893
1106	54-55	0.19145706282797903
1106	56-57	0.1868424706614462
1106	58-59	0.16886496092016756
1106	60-61	0.17535804571686242
1106	62-63	0.22317185105204373
1106	64-65	0.174574929492735
1106	66-67	0.2686966325725564
1106	68-69	0.21770430427606158
1106	70-71	0.2514900188427234
1106	72-73	0.1844831829098439
1106	74-75	0.19614747552920164
1106	76-77	0.22238873400799264
1106	78-79	0.2500581403701503
1106	80-81	0.24561240166961085
1106	82-83	0.25981799764633706
1106	84-85	0.22980411321463023
1106	86-87	0.19741121749395774
1106	88-89	0.2661091751244449
1106	90-91	0.2695503680330056
1106	92-93	0.3028930677659467
1106	94-95	0.3184130343000149
1106	96-97	0.256703780883921
1106	98-99	0.2917519479982431
1106	100	0.3294869600874364
1107	1	-0.016211867788854306
1107	2	0.021686466237085256
1107	3	0.04927312511001247
1107	4	-0.03019721389251373
1107	5	-0.04402281282970222
1107	6	-0.04538736001695298
1107	7	0.008279090417325108
1107	8	0.032901070054577986
1107	9	0.04859791091327281
1107	10-11	0.08438137806686541
1107	12-13	0.08943014371627811
1107	14-15	0.08014252797391208
1107	16-17	0.08624455260188313
1107	18-19	0.13082730933736286
1107	20-21	0.1542802189373944
1107	22-23	0.1464332801867414
1107	24-25	0.12066007133911683
1107	26-27	0.0871222301604746
1107	28-29	0.12229152849835856
1107	30-31	0.132201694744829
1107	32-33	0.09886142582633539
1107	34-35	0.1225273895844623
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2216	28-29	-0.3997251359664986
2216	30-31	-0.45907129244790923
2216	32-33	-0.5050708188435706
2216	34-35	-0.5116795469634283
2216	36-37	-0.598672713992265
2216	38-39	-0.6420364622205099
2216	40-41	-0.5861931535843752
2216	42-43	-0.5373155272600201
2216	44-45	-0.4738451884722963
2216	46-47	-0.5715337902459936
2216	48-49	-0.4623638097102969
2216	50-51	-0.4162244999007001
2216	52-53	-0.44712616050190235
2216	54-55	-0.4813374822265004
2216	56-57	-0.5270295472506703
2216	58-59	-0.5239916803364579
2216	60-61	-0.49210172004281816
2216	62-63	-0.5319448183153526
2216	64-65	-0.5169620428771822
2216	66-67	-0.4468317069180898
2216	68-69	-0.4866888532715663
2216	70-71	-0.4610854965755351
2216	72-73	-0.49963542418888807
2216	74-75	-0.43594936542957896
2216	76-77	-0.41575963994228715
2216	78-79	-0.4487896411758996
2216	80-81	-0.46059672018063225
2216	82-83	-0.5248382691121094
2216	84-85	-0.4931663016102874
2216	86-87	-0.5200892253707536
2216	88-89	-0.4754313688568921
2216	90-91	-0.43720376812709105
2216	92-93	-0.5800091224851087
2216	94-95	-0.5654669015650171
2216	96-97	-0.6571988486828886
2216	98-99	-0.5930358265495634
2216	100	-0.5827804000205461
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46.0
3	633.0
4	463.0
5	679.0
6	884.0
7	1454.0
8	2295.0
9	2957.0
10	4002.0
11	5615.0
12	9121.0
13	13694.0
14	13936.0
15	17984.0
16	24493.0
17	39092.0
18	48755.0
19	24470.0
20	12757.0
21	10896.0
22	10366.0
23	10464.0
24	11583.0
25	13854.0
26	16656.0
27	20525.0
28	25231.0
29	31138.0
30	39211.0
31	47787.0
32	60357.0
33	76720.0
34	108464.0
35	204594.0
36	319249.0
37	698689.0
38	1074216.0
39	440782.0
40	8175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.86092592640874	20.8661806238139	14.849452151662287	22.423441298115073
2	14.196332517062608	21.89940230907868	40.61259698646057	23.291668187398148
3	15.19248149049324	30.322424600553838	31.87736800027808	22.607725908674848
4	10.136368389600465	18.799702102402733	39.91190527296707	31.152024235029735
5	11.08540512419738	39.139938249629886	35.18125810513437	14.593398521038372
6	26.405713082371197	40.61878401187386	18.656096668672102	14.319406237082838
7	23.876693913339185	35.27661518292077	23.98491203077844	16.861778872961604
8	25.770018541332167	33.21505425244193	21.073682460351645	19.941244745874258
9	27.142442097079417	14.663468014101957	22.242530820873235	35.9515590679454
10-11	23.727980900776792	26.840598710362144	28.69560091614631	20.735819472714752
12-13	25.50320990114669	26.23313183405667	28.81972153531847	19.44393672947817
14-15	22.599583985920056	25.52576016999745	29.699993077052977	22.174662767029506
16-17	19.732846660778783	28.92554993255196	29.229913387849855	22.11169001881941
18-19	19.60123825162856	25.916761845118902	34.92332763759212	19.558672265660416
20-21	21.359492996961144	24.758254455669533	34.99578685086147	18.88646569650785
22-23	20.571989442351786	25.11321490352474	34.99050194016483	19.324293713958642
24-25	19.981655622770315	25.539048931084462	34.66788943203977	19.811406014105444
26-27	19.77205139981105	25.76851256832643	34.71501862760135	19.74441740426117
28-29	20.07616469870205	25.256864678758568	35.17852314233405	19.48844748020533
30-31	20.43888958891848	25.339135371722953	34.74764898681579	19.47432605254277
32-33	19.502527410454356	25.31686050722014	35.19513926104022	19.985472821285285
34-35	20.335883992260396	25.286009487808126	34.60954148537078	19.7685650345607
36-37	20.910460009611352	25.294402037908366	33.85638643785194	19.938751514628347
38-39	20.41051963814484	25.29420284397531	34.11647900909852	20.17879850878133
40-41	20.168288010724826	25.55681530813744	34.15017538208499	20.12472129905274
42-43	20.365286764607905	25.580631780675578	34.00386238172289	20.050219072993627
44-45	20.35600866732681	25.41722119222524	33.35271185628146	20.874058284166487
46-47	21.132439585402384	25.751350637346142	32.551500630111555	20.56470914713992
48-49	21.115611939502994	25.744422983114557	32.068151244827945	21.071813832554508
50-51	21.462219232225916	26.200009993475277	30.900346947391593	21.437423826907214
52-53	21.165091768179114	26.42643164558209	31.06192534453982	21.34655124169898
54-55	21.242053230232013	26.659970584550592	31.013543996807897	21.0844321884095
56-57	21.39630170069628	26.442050684152786	30.9168987004899	21.244748914661038
58-59	21.56648818956027	25.56485042349751	31.203658121693202	21.665003265249016
60-61	21.732123716046498	26.0521426948276	31.09681394291564	21.11891964621026
62-63	20.932326889392453	26.03497333796408	31.646789505044048	21.38591026759942
64-65	21.207115746749906	26.046777107465285	31.505607731918	21.240499413866807
66-67	21.40191500446012	25.884321981795424	31.141018059600604	21.572744954143854
68-69	21.65324092627398	26.12994409606572	30.96060365067942	21.25621132698088
70-71	21.84648299918574	25.979971710932425	30.99757344338435	21.175971846497482
72-73	21.520619172608747	25.854372662507974	31.078217916281233	21.54679024860205
74-75	21.37504891781199	25.849359498919387	31.170733954016246	21.604857629252376
76-77	21.39647455356936	26.33871259275051	30.76206046337021	21.50275239030992
78-79	21.3517766135746	26.47944437267144	30.834541726796672	21.33423728695729
80-81	21.171794866595736	26.588202885595514	30.88825233159466	21.35174991621409
82-83	21.005759728594082	26.509944540452796	31.038069129488456	21.44622660146467
84-85	21.14626858754231	25.94699970598842	30.781592847552826	22.125138858916443
86-87	21.221222801469462	26.3737923732956	30.660701522400792	21.744283302834145
88-89	20.858987581535864	26.143240103486477	30.93960628734081	22.05816602763685
90-91	20.877647556369197	26.2062747665284	30.678964961879824	22.23711271522258
92-93	20.737997236250738	26.34566215779557	31.231901910509386	21.684438695444303
94-95	21.08198233271229	25.707291116301924	30.98287033174828	22.22785621923751
96-97	20.913499035469716	26.55255773965489	30.407072478595886	22.126870746279508
98-99	20.164857120883585	26.185754220893536	31.06852250658022	22.580866151642653
100	20.580150027113334	26.227457696247235	30.71474339662868	22.477648880010754
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5908.0
1	3960.0
2	7818.0
3	10805.0
4	4936.0
5	2762.0
6	6517.0
7	18029.0
8	30983.0
9	30134.0
10	16205.0
11	5867.5
12	4166.5
13	4115.0
14	4286.0
15	4636.5
16	4996.0
17	5507.0
18	6217.5
19	6926.0
20	7904.0
21	9121.0
22	10630.5
23	12928.0
24	16201.5
25	19889.5
26	24305.5
27	29880.5
28	36501.5
29	43005.0
30	47862.5
31	52340.5
32	58432.5
33	63479.0
34	67578.0
35	76290.5
36	86513.0
37	95070.5
38	108189.5
39	118103.0
40	124304.0
41	131754.0
42	132467.5
43	135342.0
44	138157.0
45	132216.5
46	128525.5
47	125797.0
48	114761.5
49	111571.0
50	114995.5
51	108625.0
52	97026.5
53	86652.0
54	79635.0
55	74413.5
56	66418.0
57	56946.5
58	52562.0
59	50496.5
60	45790.0
61	38808.0
62	32459.5
63	25720.0
64	19434.5
65	15390.0
66	12208.0
67	9294.5
68	6735.0
69	4706.0
70	3305.0
71	2531.0
72	1960.5
73	1387.0
74	1169.5
75	975.5
76	608.0
77	436.0
78	310.0
79	165.5
80	68.5
81	33.0
82	16.0
83	6.5
84	4.5
85	4.5
86	4.0
87	4.0
88	2.0
89	0.5
90	2.0
91	2.0
92	1.0
93	1.0
94	2.5
95	5.0
96	7.0
97	9.5
98	6.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009500948212011341
2	2.8966305524424824E-5
3	2.0276413867097376E-4
4	0.0027228327192959337
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.689891657327448E-5
24-25	8.689891657327448E-5
26-27	5.069103466774344E-4
28-29	9.12438624019382E-4
30-31	0.0015062478872700908
32-33	0.0039683838568462014
34-35	0.004996687702963282
36-37	0.003475956662930979
38-39	0.002230405525380711
40-41	0.0031862936076867306
42-43	3.765619718175227E-4
44-45	3.331125135308855E-4
46-47	0.0034904398156931915
48-49	0.00240420335852726
50-51	9.993375405926565E-4
52-53	2.8966305524424824E-5
54-55	5.64842957726284E-4
56-57	7.096744853484081E-4
58-59	6.807081798239834E-4
60-61	3.041462080064606E-4
62-63	0.0
64-65	0.0
66-67	6.807081798239834E-4
68-69	7.38640790872833E-4
70-71	0.0020276413867097377
72-73	7.241576381106205E-5
74-75	0.001969708775660888
76-77	4.3449458286637236E-4
78-79	0.0013034837485991172
80-81	0.0010427869988792937
82-83	0.0011876185265014176
84-85	0.0012165848320258425
86-87	2.751799024820358E-4
88-89	0.001404865817934604
90-91	0.0013614163596479668
92-93	0.0026793832610092962
94-95	8.979554712571695E-4
96-97	0.002563518038911597
98-99	0.0024766191223383224
100	0.003215259913211155
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3452287.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.36377156495562
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.61626149212765	29.623186806964956
2	15.319492753409572	12.673439974860532
3	5.4834027473693805	6.804426559225113
4	2.5023520635485226	4.140268765268655
5	1.3429835736512352	2.777543287799872
6	0.8134588523910595	2.0188635688676846
7	0.5446905420384076	1.5771318608127984
8	0.38169129168516625	1.2630553118078447
9	0.2748258431076519	1.0231050055006123
>10	1.4482061666689723	11.041680101075553
>50	0.1424606099045607	4.109557680351729
>100	0.10806245847350184	9.14265000733389
>500	0.013688136815322608	3.930134096690469
>1k	0.00793209979512572	5.464865456358655
>5k	2.1058672022457664E-4	0.6422660703280508
>10k+	2.8078229363276885E-4	3.7678254467536103
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51234	1.4840596972383813	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33774	0.978308002781924	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29293	0.8485099877269763	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15465	0.4479639149352299	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8284	0.23995687496433526	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7327	0.21223612057746066	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6509	0.18854168265848117	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4840	0.14019691873821616	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4400	0.12745174430746922	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG	4360	0.12629309208649223	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT	4177	0.12099225817552249	No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG	4019	0.11641558190266336	No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC	3571	0.10343867702772105	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	2.8966305524424824E-5
6	0.0	0.0	0.0	0.0	2.8966305524424824E-5
7	0.0	0.0	0.0	0.0	2.8966305524424824E-5
8	0.0	0.0	0.0	0.0	2.8966305524424824E-5
9	0.0	0.0	0.0	0.0	2.8966305524424824E-5
10-11	0.0	0.0	0.0	0.0	2.8966305524424824E-5
12-13	0.0	0.0	0.0	0.0	2.8966305524424824E-5
14-15	0.0	0.0	0.0	0.0	2.8966305524424824E-5
16-17	0.0	0.0	0.0	2.8966305524424824E-5	2.8966305524424824E-5
18-19	0.0	0.0	0.0	8.689891657327447E-5	2.8966305524424824E-5
20-21	0.0	0.0	0.0	2.7517990248203584E-4	4.344945828663723E-5
22-23	0.0	0.0	0.0	7.965734019216826E-4	5.793261104884965E-5
24-25	0.0	0.0	0.0	0.0015931468038433653	7.241576381106205E-5
26-27	0.0	0.0	0.0	0.0028386979413936326	8.689891657327447E-5
28-29	0.0	0.0	0.0	0.006343620909849036	8.689891657327447E-5
30-31	0.0	0.0	0.0	0.013686579360290729	8.689891657327447E-5
32-33	0.0	0.0	0.0	0.024534460779187826	8.689891657327447E-5
34-35	0.0	0.0	0.0	0.03893071462482696	8.689891657327447E-5
36-37	0.0	0.0	0.0	0.06017749972699257	8.689891657327447E-5
38-39	0.0	0.0	0.0	0.09256182930329952	8.689891657327447E-5
40-41	0.0	0.0	0.0	0.12955180145799003	8.689891657327447E-5
42-43	0.0	0.0	0.0	0.16341341261604264	8.689891657327447E-5
44-45	0.0	0.0	0.0	0.20259034083782723	8.689891657327447E-5
46-47	0.0	0.0	0.0	0.2490233285934802	8.689891657327447E-5
48-49	0.0	0.0	0.0	0.2996129811918882	8.689891657327447E-5
50-51	0.0	0.0	0.0	0.35731386179654245	8.689891657327447E-5
52-53	0.0	0.0	0.0	0.4149857760956722	8.689891657327447E-5
54-55	0.0	0.0	0.0	0.47538052311409795	8.689891657327447E-5
56-57	0.0	0.0	0.0	0.5460727917464567	8.689891657327447E-5
58-59	0.0	0.0	0.0	0.6254984014944296	8.689891657327447E-5
60-61	0.0	0.0	0.0	0.7179588487283937	8.689891657327447E-5
62-63	0.0	0.0	0.0	0.8126641846405007	8.689891657327447E-5
64-65	0.0	0.0	0.0	0.9097302744528482	8.689891657327447E-5
66-67	0.0	0.0	0.0	1.0127634232032272	8.689891657327447E-5
68-69	0.0	0.0	0.0	1.142503505647126	8.689891657327447E-5
70-71	0.0	0.0	0.0	1.2903764953493149	8.689891657327447E-5
72-73	0.0	0.0	0.0	1.4477359501107527	8.689891657327447E-5
74-75	0.0	0.0	0.0	1.6058340456630633	8.689891657327447E-5
76-77	0.0	0.0	0.0	1.7742731122875939	8.689891657327447E-5
78-79	0.0	0.0	0.0	1.9615113111974756	8.689891657327447E-5
80-81	0.0	0.0	0.0	2.1826256044181727	8.689891657327447E-5
82-83	0.0	0.0	0.0	2.422524546771459	8.689891657327447E-5
84-85	0.0	0.0	0.0	2.67089613349064	8.689891657327447E-5
86-87	0.0	0.0	0.0	2.930072152170431	8.689891657327447E-5
88	0.0	0.0	0.0	3.1331114707438865	8.689891657327447E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11240	0.0	66.57518	1
GTATCAA	25130	0.0	51.10034	1
ATCAACG	34905	0.0	36.638058	3
TCAACGC	35285	0.0	36.256287	4
CAACGCA	36520	0.0	35.01733	5
TATCAAC	37005	0.0	34.68589	2
AACGCAG	37255	0.0	34.30125	6
ACGCAGA	41285	0.0	30.861889	7
CGCAGAG	41785	0.0	30.436357	8
TAACGCA	245	0.0	28.774675	4
GCAGAGT	43810	0.0	28.77205	9
GTGGTAT	3110	0.0	28.414045	1
TGGTATC	3440	0.0	25.54905	2
TATAACG	260	1.8189894E-12	25.307327	2
TACATGG	20635	0.0	24.849167	2
GTACATG	22295	0.0	24.266293	1
AGAGTAC	43115	0.0	23.676456	10-11
AGTACTT	32705	0.0	23.653803	12-13
ACATGGG	21120	0.0	23.010084	3
GAGTACT	32510	0.0	22.776485	12-13
>>END_MODULE