FastQCFastQC Report
Wed 25 May 2016
SRR2431361_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431361_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7697391
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240590.31256045067737886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69100.041.4223331
GTATCAA129350.037.8613931
TCAACGC130700.035.7442444
ATCAACG132850.035.0596353
CAACGCA141300.033.0295335
TATCAAC153100.031.2512842
AACGCAG149800.031.0926076
ACGCAGA167400.027.205937
CGCAGAG168000.026.9409128
GTACATG272100.026.7039951
TACATGG272300.025.7695882
ACATGGG279950.024.1756043
GCAGAGT202200.021.3149099
CATGGGG175950.020.5949344
GAGTACA192150.017.4888381
CATGGGT58700.017.3746854
AGAGTAC177350.017.21247310-11
CAGAGTA195750.016.69900710-11
CATGGGA83350.015.7323624
AGTACAT210650.015.2612452
Produced by FastQC (version 0.11.5)