FastQCFastQC Report
Wed 25 May 2016
SRR2431361_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431361_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7697391
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370140.4808642304905649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237110.3080394382979895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210330.273248429240505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121850.059.559841
GTATCAA267950.049.2402731
ATCAACG346850.037.4526863
TCAACGC349000.037.221964
TATCAAC358150.036.507222
CAACGCA359800.036.104215
AACGCAG372600.034.914376
ACGCAGA420700.030.8219557
CGCAGAG424700.030.38788
GCAGAGT467150.027.4151829
GTACATG251300.026.5786781
TACATGG255400.025.2659872
ACATGGG260800.024.184193
AGAGTAC442700.023.19652410-11
GAGTACT326600.021.85861412-13
AGTACTT349200.021.79654712-13
CAGAGTA463800.021.18869810-11
CATGGGG169050.020.990294
GTGGTAT51300.020.524021
GTACTTT373400.018.91129114-15
Produced by FastQC (version 0.11.5)