##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431361_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7697391
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.765421816301135	34.0	31.0	34.0	31.0	34.0
2	32.89741186331836	34.0	31.0	34.0	31.0	34.0
3	32.970866752124195	34.0	31.0	34.0	31.0	34.0
4	36.352955566373076	37.0	37.0	37.0	35.0	37.0
5	36.30010869916833	37.0	37.0	37.0	35.0	37.0
6	36.32609269816227	37.0	37.0	37.0	35.0	37.0
7	36.19217914225742	37.0	37.0	37.0	35.0	37.0
8	36.246561724615525	37.0	37.0	37.0	35.0	37.0
9	38.070962875602916	39.0	39.0	39.0	35.0	39.0
10-11	38.06216541422931	39.0	39.0	39.0	35.0	39.0
12-13	38.04085306307033	39.0	39.0	39.0	35.0	39.0
14-15	39.54300626017309	41.0	40.0	41.0	37.0	41.0
16-17	39.559709192374406	41.0	40.0	41.0	37.0	41.0
18-19	39.625269263312724	41.0	40.0	41.0	37.0	41.0
20-21	39.63054293331338	41.0	40.0	41.0	37.0	41.0
22-23	39.56129265097746	41.0	40.0	41.0	36.5	41.0
24-25	39.535938930476576	41.0	40.0	41.0	36.0	41.0
26-27	39.45287520148061	41.0	40.0	41.0	36.0	41.0
28-29	39.37051710118402	41.0	39.0	41.0	36.0	41.0
30-31	39.22350443156648	41.0	39.0	41.0	35.5	41.0
32-33	39.043058355746766	41.0	39.0	41.0	35.0	41.0
34-35	38.944100345168906	41.0	39.0	41.0	35.0	41.0
36-37	38.83651408639629	41.0	39.0	41.0	35.0	41.0
38-39	38.7228429736777	41.0	39.0	41.0	35.0	41.0
40-41	38.63464854520187	41.0	38.5	41.0	35.0	41.0
42-43	38.519406900338055	41.0	38.0	41.0	35.0	41.0
44-45	38.343449618188814	41.0	38.0	41.0	34.5	41.0
46-47	38.0878161834315	41.0	38.0	41.0	34.0	41.0
48-49	37.873247182064674	40.0	38.0	41.0	33.0	41.0
50-51	37.74968687442278	40.0	38.0	41.0	33.0	41.0
52-53	37.65036601362722	40.0	38.0	41.0	33.0	41.0
54-55	37.519672704686556	40.0	37.0	41.0	33.0	41.0
56-57	37.370761144912606	40.0	37.0	41.0	33.0	41.0
58-59	37.20245340271788	40.0	37.0	41.0	33.0	41.0
60-61	37.0216228459747	40.0	36.0	41.0	32.0	41.0
62-63	36.80671249518181	39.5	36.0	41.0	32.0	41.0
64-65	36.5500601437552	39.0	35.0	41.0	31.5	41.0
66-67	36.27862681004512	39.0	35.0	41.0	31.0	41.0
68-69	36.015669660018574	38.5	35.0	41.0	31.0	41.0
70-71	35.76467786292784	37.5	35.0	40.0	31.0	41.0
72-73	35.50344207537333	37.0	35.0	40.0	31.0	41.0
74-75	35.19340462242336	36.5	35.0	39.0	31.0	41.0
76-77	34.8588478225934	36.0	35.0	39.0	31.0	41.0
78-79	34.526607067251746	36.0	35.0	38.5	31.0	40.0
80-81	34.197027018635275	35.0	35.0	37.0	30.5	39.0
82-83	33.85725676401263	35.0	35.0	37.0	30.0	39.0
84-85	33.57286442899938	35.0	34.5	36.5	30.0	39.0
86-87	33.32676474405419	35.0	34.0	36.0	30.0	37.0
88-89	33.086653321885294	35.0	34.0	36.0	30.0	37.0
90-91	32.873740466087796	35.0	34.0	35.5	29.0	37.0
92-93	32.714222585288965	35.0	34.0	35.0	29.0	36.0
94-95	32.57051278803428	35.0	34.0	35.0	29.0	36.0
96-97	32.43027559597791	35.0	34.0	35.0	29.0	36.0
98-99	32.30578568764403	35.0	34.0	35.0	29.0	36.0
100	32.20092353370122	35.0	34.0	35.0	29.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1704336858842126
1101	2	0.1650355769156846
1101	3	0.17935846840477154
1101	4	0.11395308610186561
1101	5	0.14076221867997418
1101	6	0.1563447174215753
1101	7	0.18969075140297065
1101	8	0.17950997449514006
1101	9	0.25071849101036037
1101	10-11	0.2025899255791188
1101	12-13	0.21523678363737986
1101	14-15	0.29492788558581395
1101	16-17	0.30914233902229427
1101	18-19	0.3096866056999872
1101	20-21	0.32136850915563286
1101	22-23	0.3220621608500096
1101	24-25	0.2967756826791188
1101	26-27	0.3121307549237997
1101	28-29	0.31655028913440475
1101	30-31	0.37802689577174675
1101	32-33	0.37952437381985504
1101	34-35	0.33196944353011304
1101	36-37	0.2992029733749533
1101	38-39	0.33862089555835695
1101	40-41	0.33806533973899633
1101	42-43	0.3462486485017493
1101	44-45	0.3595104483641691
1101	46-47	0.3773880196607493
1101	48-49	0.33985184725328565
1101	50-51	0.32582252354301744
1101	52-53	0.2943192723850885
1101	54-55	0.3020452608379074
1101	56-57	0.3423671973380422
1101	58-59	0.33963953189735463
1101	60-61	0.2619257829357622
1101	62-63	0.2577607747076911
1101	64-65	0.3135092881791337
1101	66-67	0.3395240302546725
1101	68-69	0.31690396305879176
1101	70-71	0.28117345667707383
1101	72-73	0.2819697240950916
1101	74-75	0.2928463368252423
1101	76-77	0.28757204515957113
1101	78-79	0.30186206612758326
1101	80-81	0.3162727156321239
1101	82-83	0.33379690401172013
1101	84-85	0.3161443543499942
1101	86-87	0.3000992483546838
1101	88-89	0.3005680187153459
1101	90-91	0.31284937031670523
1101	92-93	0.3011188291303597
1101	94-95	0.2801970542505785
1101	96-97	0.2980267206640619
1101	98-99	0.3079068375427312
1101	100	0.34671460632262097
1102	1	0.06833799745235325
1102	2	0.11977151771007755
1102	3	0.14483224839531772
1102	4	0.07976215870462511
1102	5	0.09299105360365445
1102	6	0.08337572769601564
1102	7	0.12108671526104331
1102	8	0.13761539101450637
1102	9	0.15980895361644087
1102	10-11	0.18949879225394994
1102	12-13	0.19224574980727738
1102	14-15	0.25063993919541616
1102	16-17	0.27545654265455966
1102	18-19	0.23742084859548385
1102	20-21	0.21982893925589053
1102	22-23	0.2504260165708061
1102	24-25	0.24262407673678155
1102	26-27	0.25676707882226424
1102	28-29	0.19681810806173416
1102	30-31	0.18059911905879034
1102	32-33	0.25211036155722155
1102	34-35	0.22804051006851012
1102	36-37	0.22441897832356972
1102	38-39	0.21180965112561267
1102	40-41	0.26266907631470104
1102	42-43	0.2764559233256634
1102	44-45	0.30075616609166644
1102	46-47	0.28970708137959633
1102	48-49	0.33130641628405044
1102	50-51	0.32703776473331203
1102	52-53	0.3292870814830593
1102	54-55	0.3488114516959584
1102	56-57	0.32774428029131286
1102	58-59	0.32920935383533845
1102	60-61	0.3178950590413976
1102	62-63	0.3175433830395775
1102	64-65	0.3210031542001772
1102	66-67	0.30850817931609953
1102	68-69	0.3248102932099499
1102	70-71	0.2812036651408292
1102	72-73	0.26581661302576265
1102	74-75	0.27012293718272673
1102	76-77	0.2713634243124403
1102	78-79	0.263703421634105
1102	80-81	0.2267827919641121
1102	82-83	0.23662534995781925
1102	84-85	0.24183679207839504
1102	86-87	0.2011110030573775
1102	88-89	0.2175422753980527
1102	90-91	0.2656862609110462
1102	92-93	0.2576909642644125
1102	94-95	0.23259206291712076
1102	96-97	0.26569385433408144
1102	98-99	0.26423865202747265
1102	100	0.2928118886703288
1103	1	0.1035947167115836
1103	2	0.13062709728561117
1103	3	0.11315563237750581
1103	4	0.0970593492036329
1103	5	0.10921299330370005
1103	6	0.12279195528148534
1103	7	0.12867631571625537
1103	8	0.1267129220079326
1103	9	0.1557488849489701
1103	10-11	0.17140334175359584
1103	12-13	0.14785311835111514
1103	14-15	0.23768012125866278
1103	16-17	0.2514671507871853
1103	18-19	0.21172706849103662
1103	20-21	0.24077580318578384
1103	22-23	0.24554248984236438
1103	24-25	0.19664955317735888
1103	26-27	0.20928022082517828
1103	28-29	0.21385352428520576
1103	30-31	0.24874184848304992
1103	32-33	0.23805212363148343
1103	34-35	0.21217573908744214
1103	36-37	0.21377602929295847
1103	38-39	0.20457040639511348
1103	40-41	0.24983304447206933
1103	42-43	0.2267243590564192
1103	44-45	0.24389132169557115
1103	46-47	0.2390622126876636
1103	48-49	0.1480270771825687
1103	50-51	0.18954724500229503
1103	52-53	0.1652234921006226
1103	54-55	0.19942452975441682
1103	56-57	0.14643248132994557
1103	58-59	0.1505456555301521
1103	60-61	0.09690757513804016
1103	62-63	0.09822673588136155
1103	64-65	0.15055988451298674
1103	66-67	0.16717801131993326
1103	68-69	0.13396677714284522
1103	70-71	0.14510126794120737
1103	72-73	0.14699416093345263
1103	74-75	0.09035634786106783
1103	76-77	0.11662654853769538
1103	78-79	0.055117829048001
1103	80-81	0.03730822435817771
1103	82-83	0.08999096536194173
1103	84-85	0.07599003921281877
1103	86-87	0.06821622366700808
1103	88-89	0.07527208952929954
1103	90-91	0.12729616293692914
1103	92-93	0.1439642060616677
1103	94-95	0.1644040471192909
1103	96-97	0.15284947907826307
1103	98-99	0.14411072253074053
1103	100	0.18044159261391712
1104	1	0.09462159456825248
1104	2	0.13463872158513368
1104	3	0.137394985909161
1104	4	0.06017404821233896
1104	5	0.08040814646685845
1104	6	0.10710946670965171
1104	7	0.0845890435415626
1104	8	0.12099364042347105
1104	9	0.16373998247426158
1104	10-11	0.16809153208194516
1104	12-13	0.19042497003950132
1104	14-15	0.21368263983838176
1104	16-17	0.2375757269150256
1104	18-19	0.2252463500134283
1104	20-21	0.22303686676878698
1104	22-23	0.22418500193078472
1104	24-25	0.2371877902443984
1104	26-27	0.21428889998180978
1104	28-29	0.18318859859468972
1104	30-31	0.2517792021509564
1104	32-33	0.22811600863983728
1104	34-35	0.24061495218660411
1104	36-37	0.2697651112227675
1104	38-39	0.23570465446528743
1104	40-41	0.2369013019774613
1104	42-43	0.2412288337558124
1104	44-45	0.21235897849118146
1104	46-47	0.2418899065658664
1104	48-49	0.2748528633269558
1104	50-51	0.2549962801932466
1104	52-53	0.24818857028537877
1104	54-55	0.24086453216774828
1104	56-57	0.24707105048972977
1104	58-59	0.237931285863759
1104	60-61	0.24688745070546503
1104	62-63	0.22845080559122977
1104	64-65	0.303378356088011
1104	66-67	0.28927088093367814
1104	68-69	0.2904939938964972
1104	70-71	0.26154857804779397
1104	72-73	0.2683704176570245
1104	74-75	0.25951907134594165
1104	76-77	0.24311946041414245
1104	78-79	0.2058604616870241
1104	80-81	0.20060226217061938
1104	82-83	0.23949919521201934
1104	84-85	0.21477947509352902
1104	86-87	0.20247741722625534
1104	88-89	0.20164036597581259
1104	90-91	0.2343477035172441
1104	92-93	0.26411459795605197
1104	94-95	0.24119237893685863
1104	96-97	0.2335210922247839
1104	98-99	0.27254652452919004
1104	100	0.2539862796093715
1105	1	-0.05897945628395007
1105	2	0.02562818156565072
1105	3	0.03873437875169117
1105	4	-0.07247661525666871
1105	5	-0.09962680826352965
1105	6	-0.09976170946949026
1105	7	-0.47478798630314145
1105	8	-0.3903492827530499
1105	9	-0.3430972374159751
1105	10-11	-0.32066964295278666
1105	12-13	-0.18055070893012015
1105	14-15	-0.16143696064320068
1105	16-17	-0.1521408237337667
1105	18-19	-0.1518159904291636
1105	20-21	-0.14224436127381068
1105	22-23	-0.11293241384287
1105	24-25	-0.12397051735786135
1105	26-27	-0.1424576582407937
1105	28-29	-0.2036236985326738
1105	30-31	-0.17177429167099234
1105	32-33	-0.21263912743111746
1105	34-35	-0.19955737722226985
1105	36-37	-0.18632061245088494
1105	38-39	-0.18711535104122134
1105	40-41	-0.19865044786911312
1105	42-43	-0.15591495505588426
1105	44-45	-0.18497395251629456
1105	46-47	-0.17899512979034427
1105	48-49	-0.20502264232044354
1105	50-51	-0.21172435301416925
1105	52-53	-0.20577891571468854
1105	54-55	-0.2355212457044047
1105	56-57	-0.21928504222270107
1105	58-59	-0.18623985071738502
1105	60-61	-0.20441523126972783
1105	62-63	-0.2223061757573177
1105	64-65	-0.2140666417740249
1105	66-67	-0.22758358916222932
1105	68-69	-0.24148576279362288
1105	70-71	-0.2791407372680581
1105	72-73	-0.2352736866346845
1105	74-75	-0.2616515663292702
1105	76-77	-0.23627122700320768
1105	78-79	-0.22937765972991997
1105	80-81	-0.21187421020871255
1105	82-83	-0.2271717768814412
1105	84-85	-0.21089139896179177
1105	86-87	-0.21910225339177458
1105	88-89	-0.17358986086077977
1105	90-91	-0.1428569637145216
1105	92-93	-0.14012475706466887
1105	94-95	-0.17702194658419046
1105	96-97	-0.1562610712395056
1105	98-99	-0.1712621259339926
1105	100	-0.2057572176925433
1106	1	0.09522251067823362
1106	2	0.09842761083704943
1106	3	0.11023045515661067
1106	4	0.07543834821936457
1106	5	0.06803871468387257
1106	6	0.10451469485161624
1106	7	0.10238781883854386
1106	8	0.12586513895760731
1106	9	0.12732605462711177
1106	10-11	0.1304771358837229
1106	12-13	0.12632863309292475
1106	14-15	0.17454067281211394
1106	16-17	0.21992076820122008
1106	18-19	0.18573738922908234
1106	20-21	0.16174439559027576
1106	22-23	0.19622774687771738
1106	24-25	0.19615562904058237
1106	26-27	0.17615121517229682
1106	28-29	0.2048708360726934
1106	30-31	0.18932520446890067
1106	32-33	0.18496641461764085
1106	34-35	0.18161953008410592
1106	36-37	0.18107750995738314
1106	38-39	0.14874189522922876
1106	40-41	0.15966411092163924
1106	42-43	0.16358991926403377
1106	44-45	0.13127294190440608
1106	46-47	0.15390612192705788
1106	48-49	0.08716042971587257
1106	50-51	0.12254169687643923
1106	52-53	0.09204561892254759
1106	54-55	0.0884220434292331
1106	56-57	0.08806116384185003
1106	58-59	0.08473054630623267
1106	60-61	0.08730339032702972
1106	62-63	0.07743089238211809
1106	64-65	0.09731380231998799
1106	66-67	0.15154923942991871
1106	68-69	0.14262968145941812
1106	70-71	0.13521375730869778
1106	72-73	0.10945169170406643
1106	74-75	0.11162974208732379
1106	76-77	0.13412856093101055
1106	78-79	0.11639190608354966
1106	80-81	0.11864221921884877
1106	82-83	0.11696082784716566
1106	84-85	0.12493517737960502
1106	86-87	0.1143764076799556
1106	88-89	0.12287237903181847
1106	90-91	0.1469550919913516
1106	92-93	0.12894887688506174
1106	94-95	0.1285274578013329
1106	96-97	0.16002814323100978
1106	98-99	0.16156347225030032
1106	100	0.15098825768643565
1107	1	-0.009483887342476294
1107	2	0.028265757046405326
1107	3	0.005260948550827038
1107	4	-0.07304903172943966
1107	5	-0.07870806835492772
1107	6	-0.06583884069493706
1107	7	-0.0259918605348588
1107	8	-0.010648763055954191
1107	9	-2.281403688186856E-5
1107	10-11	0.019698579230599478
1107	12-13	0.06086682090283091
1107	14-15	0.04346956093765897
1107	16-17	0.0751280223434847
1107	18-19	0.06486167179944147
1107	20-21	0.07429388686871619
1107	22-23	0.055286754183612175
1107	24-25	0.057832908193759636
1107	26-27	0.024326440656608384
1107	28-29	0.030546554165510997
1107	30-31	0.06418514965868383
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2216	34-35	-0.530560136088468
2216	36-37	-0.5582523944016629
2216	38-39	-0.5427782627432052
2216	40-41	-0.5600851174848813
2216	42-43	-0.5119769185047858
2216	44-45	-0.4999233596948187
2216	46-47	-0.4857545254688276
2216	48-49	-0.48574645278903006
2216	50-51	-0.4407884654230614
2216	52-53	-0.48208123282753945
2216	54-55	-0.5227157363816985
2216	56-57	-0.5335624401358743
2216	58-59	-0.5975540189527067
2216	60-61	-0.594841275652449
2216	62-63	-0.6487703996965877
2216	64-65	-0.5525588364851473
2216	66-67	-0.506312845105235
2216	68-69	-0.5440012176289315
2216	70-71	-0.5320032854757599
2216	72-73	-0.5495357756573043
2216	74-75	-0.47249945637631896
2216	76-77	-0.4242602593057754
2216	78-79	-0.39153257182218937
2216	80-81	-0.40554231019577713
2216	82-83	-0.43125281071736055
2216	84-85	-0.38021873039355825
2216	86-87	-0.34408506661065275
2216	88-89	-0.3206379801737853
2216	90-91	-0.3242207674553583
2216	92-93	-0.45630947944508193
2216	94-95	-0.4179625840137362
2216	96-97	-0.5313871552620384
2216	98-99	-0.5407032228343738
2216	100	-0.5120355570004236
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	98.0
3	536.0
4	306.0
5	526.0
6	721.0
7	1212.0
8	2190.0
9	3427.0
10	5047.0
11	7399.0
12	11558.0
13	17075.0
14	18664.0
15	22997.0
16	29152.0
17	43247.0
18	51476.0
19	27614.0
20	17738.0
21	16721.0
22	16660.0
23	17316.0
24	19988.0
25	24288.0
26	30351.0
27	39261.0
28	49099.0
29	61653.0
30	76764.0
31	97140.0
32	121127.0
33	157279.0
34	215550.0
35	339459.0
36	586085.0
37	1308791.0
38	2581428.0
39	1653940.0
40	23508.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.86771517478383	21.72237272059922	14.686623401090085	24.72328870352686
2	12.706149395295107	22.535895604107935	41.365860198604956	23.392094801992
3	14.636099496386102	29.077405060043276	32.28754255514712	23.998952888423506
4	10.090983757985699	18.836750724094664	41.104841008377335	29.9674245095423
5	11.433146633710045	38.8175422035856	36.506967100930694	13.24234406177366
6	27.31411201535689	40.89015355982306	18.973285883489613	12.822448541330434
7	23.673332951385735	34.39810969716882	25.241214847991998	16.687342503453443
8	23.631305724238253	36.05400842960946	22.354353572528666	17.96033227362362
9	25.203669658979255	15.950079708826017	22.60607522730754	36.24017540488719
10-11	22.332755345285175	27.373372354347076	30.778110401303504	19.51576189906424
12-13	23.70777968794881	25.671269654873967	30.591755050509974	20.029195606667248
14-15	21.134141425321904	26.61525184312451	28.6347348081967	23.615871923356888
16-17	19.7379280849836	29.966517226421264	28.926547969305442	21.36900671928969
18-19	19.62746857993832	28.33802388367695	32.1428975090391	19.891610027345628
20-21	21.038894867104972	27.66198962739453	31.570261923812886	19.728853581687613
22-23	20.476416372340502	27.712926384280273	31.71444794007627	20.09620930330296
24-25	20.38870091375855	27.6050816488486	31.631800598216266	20.374416839176586
26-27	20.290145121278673	27.751617276324144	31.606915622314418	20.35132198008277
28-29	20.19051304931372	27.66128161091902	31.816279444255958	20.3319258955113
30-31	20.63565919973923	27.877807324391746	31.316358490726593	20.170174985142427
32-33	20.05374923696312	27.81884515468577	31.80890522908191	20.318500379269196
34-35	20.416162327187195	27.548222519612803	31.531723253702427	20.503891899497575
36-37	20.692349211605794	27.908491666861007	31.038656984973308	20.360502136559894
38-39	20.523276540327952	27.95701991063754	31.11871186271565	20.400991686318857
40-41	20.53846380704261	27.789137728025253	31.06116717176907	20.611231293163065
42-43	20.383047163603603	27.8961279556419	31.07632426983035	20.64450061092414
44-45	20.708255392553582	27.797798736071588	30.810867973337057	20.683077898037773
46-47	20.809404420155943	28.12280436901983	30.372968846464854	20.694822364359375
48-49	20.76366240853651	28.09066729096319	30.147844728412178	20.997825572088125
50-51	20.951027047111264	28.156109082263804	29.72727485316299	21.165589017461944
52-53	20.752160245506627	28.302538692016217	29.884821983142594	21.060479079334566
54-55	20.99035414472323	28.390903489806067	29.635741403126207	20.983000962344494
56-57	20.862919607160062	28.243033772669456	29.773953538585797	21.12009308158468
58-59	20.717203238005904	27.94870429096843	30.05407069724177	21.280021773783897
60-61	21.06571466933373	28.303176134208602	29.574137783984522	21.056971412473146
62-63	20.796663440898353	28.21601501080041	29.94467865800243	21.042642890298804
64-65	20.847485855921832	27.90395472959604	29.975188995855866	21.273370418626257
66-67	20.819130895925067	28.158417030115253	29.77625095666302	21.24620111729666
68-69	21.023916918761543	28.14112835308487	29.643499271533447	21.19145545662014
70-71	21.109625579869274	28.047477988450098	29.551529651851734	21.291366779828895
72-73	20.873555620642634	28.181528768130924	29.7185061853185	21.226409425907946
74-75	21.044270413810665	28.048300429764435	29.551150802105692	21.356278354319212
76-77	21.05607944327969	28.16982458674825	29.439784986188783	21.334310983783283
78-79	20.992905958643966	28.232082527585955	29.51319325400254	21.261818259767544
80-81	21.06128290923906	28.160144715200104	29.454555351725737	21.324017023835097
82-83	20.840306297470857	28.186964799357593	29.740797684795105	21.231931218376445
84-85	20.906380335906164	28.02022034094169	29.435950807936056	21.637448515216086
86-87	20.946518403856903	28.28887278670273	29.38425377247481	21.38035503696556
88-89	20.6664044089438	28.103898749464584	29.561131015011437	21.668565826580174
90-91	20.778818459762803	28.048298291487107	29.40308093170164	21.76980231704845
92-93	20.73870785562903	28.194240382438	29.510865265202945	21.55618649673002
94-95	20.65073580327237	27.882555472034227	29.543949295480417	21.92275942921299
96-97	20.703116854788142	28.150547241311237	29.343851532476396	21.802484371424228
98-99	20.492479115293815	28.065778988838737	29.44054522687115	22.0011966689963
100	20.66847210737024	28.059640605136604	29.19000065609083	22.081886631402327
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1760.0
1	1259.0
2	2300.0
3	3464.0
4	2037.0
5	1543.5
6	3570.5
7	9903.5
8	17338.0
9	17381.5
10	10770.5
11	6074.5
12	5452.0
13	5855.5
14	6518.5
15	7191.5
16	8184.0
17	9713.0
18	11452.0
19	13400.0
20	17195.0
21	22113.0
22	27090.5
23	34689.0
24	46434.5
25	58691.0
26	72967.0
27	93410.5
28	118192.5
29	145279.5
30	168769.5
31	189330.0
32	207950.0
33	223129.0
34	237117.5
35	250113.5
36	260306.5
37	265905.0
38	271862.5
39	276624.5
40	279218.0
41	279553.5
42	273730.5
43	267899.5
44	261390.0
45	251363.0
46	242333.5
47	231230.0
48	219238.0
49	211967.0
50	207419.0
51	200638.5
52	190178.5
53	180521.0
54	171637.0
55	161349.0
56	144943.5
57	125741.0
58	112823.5
59	103559.0
60	91158.5
61	76339.5
62	63401.5
63	51448.5
64	40795.0
65	32095.5
66	24893.0
67	18855.5
68	14074.5
69	10375.5
70	7436.0
71	5367.0
72	4042.0
73	3030.5
74	2212.5
75	1465.0
76	886.0
77	534.0
78	329.0
79	177.5
80	80.0
81	35.0
82	18.0
83	15.5
84	10.5
85	7.0
86	5.5
87	4.5
88	4.0
89	4.0
90	5.5
91	6.0
92	4.0
93	4.0
94	6.0
95	14.5
96	23.5
97	21.5
98	10.5
99	2.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009626638428527276
2	0.0
3	3.3777678696586934E-4
4	0.0025073430724774147
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.444419674146734E-5
24-25	9.743561162477E-5
26-27	4.67690935798896E-4
28-29	9.158947492728381E-4
30-31	0.0017863195464541167
32-33	0.004566482331480887
34-35	0.00574870108586143
36-37	0.004176739884981808
38-39	0.0025852915617772306
40-41	0.0035336648482583253
42-43	4.67690935798896E-4
44-45	5.26152302773758E-4
46-47	0.0037285360715078658
48-49	0.0025203344873607173
50-51	0.001052304605547516
52-53	2.5982829766605333E-5
54-55	5.651265474236661E-4
56-57	7.470063557899033E-4
58-59	7.924763078814626E-4
60-61	3.962381539407313E-4
62-63	0.0
64-65	0.0
66-67	8.054677227647653E-4
68-69	7.470063557899033E-4
70-71	0.0023644375087610854
72-73	1.3640985627467802E-4
74-75	0.0019617036473787027
76-77	3.2478537208256665E-4
78-79	0.0011172616799640293
80-81	8.509376748563248E-4
82-83	0.0013316200255385235
84-85	0.0012017058767054968
86-87	3.0529824975761267E-4
88-89	0.0016629011050627413
90-91	0.0018382852059873275
92-93	0.003221870891059061
94-95	0.0011562359246139375
96-97	0.0025008473650357634
98-99	0.002487855950152461
100	0.003715544656624563
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7697391.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.683780415763124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.70083826701816	36.72103658054204
2	17.172658460811014	18.094411350085288
3	5.972080942637499	9.438954030212331
4	2.646395431735173	5.57688463275258
5	1.3602143835636533	3.5830617951015054
6	0.8102405126739073	2.561191995220048
7	0.5146485104467193	1.8979540380972173
8	0.3454975131615387	1.4561692094075789
9	0.24010259643284546	1.1384561220950358
>10	1.1164649276801666	10.434157493991444
>50	0.07622410021848927	2.7851855943117476
>100	0.04228739723842183	4.037612596211019
>500	0.0016553303013365737	0.5751554152997831
>1k	5.681214326862718E-4	0.5005077725793436
>5k	4.940185933357386E-5	0.1353131621144494
>10k+	7.41027890003608E-5	1.0639482119785686
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37014	0.4808642304905649	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23711	0.3080394382979895	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21033	0.273248429240505	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.2991414883302667E-5	0.0
3	0.0	0.0	0.0	1.2991414883302667E-5	0.0
4	0.0	0.0	0.0	1.2991414883302667E-5	0.0
5	0.0	0.0	0.0	2.5982829766605333E-5	0.0
6	0.0	0.0	0.0	2.5982829766605333E-5	0.0
7	0.0	0.0	0.0	2.5982829766605333E-5	0.0
8	1.2991414883302667E-5	0.0	0.0	2.5982829766605333E-5	0.0
9	1.2991414883302667E-5	0.0	0.0	2.5982829766605333E-5	0.0
10-11	1.2991414883302667E-5	0.0	0.0	2.5982829766605333E-5	0.0
12-13	1.2991414883302667E-5	0.0	0.0	3.247853720825667E-5	0.0
14-15	1.2991414883302667E-5	0.0	0.0	4.5469952091559334E-5	0.0
16-17	1.2991414883302667E-5	0.0	0.0	5.1965659533210666E-5	0.0
18-19	1.2991414883302667E-5	0.0	0.0	5.1965659533210666E-5	0.0
20-21	1.2991414883302667E-5	0.0	0.0	1.1692273394972401E-4	0.0
22-23	1.2991414883302667E-5	0.0	0.0	3.2478537208256665E-4	0.0
24-25	1.2991414883302667E-5	0.0	0.0	6.755535739317387E-4	0.0
26-27	1.2991414883302667E-5	0.0	0.0	0.001052304605547516	0.0
28-29	1.2991414883302667E-5	0.0	0.0	0.002487855950152461	0.0
30-31	1.2991414883302667E-5	0.0	0.0	0.006560664516067847	0.0
32-33	1.2991414883302667E-5	0.0	0.0	0.015109015509281003	0.0
34-35	1.2991414883302667E-5	0.0	0.0	0.02718453564331083	0.0
36-37	1.2991414883302667E-5	0.0	0.0	0.04506721823017695	0.0
38-39	1.2991414883302667E-5	0.0	0.0	0.07013415324750945	0.0
40-41	1.2991414883302667E-5	0.0	0.0	0.09761749143313625	0.0
42-43	1.2991414883302667E-5	0.0	0.0	0.12525672659736264	0.0
44-45	1.2991414883302667E-5	0.0	0.0	0.15874209845907528	0.0
46-47	1.2991414883302667E-5	0.0	0.0	0.19845685375733155	0.0
48-49	1.2991414883302667E-5	0.0	0.0	0.24090630188852302	0.0
50-51	1.2991414883302667E-5	0.0	0.0	0.28820804347862805	0.0
52-53	1.2991414883302667E-5	0.0	0.0	0.3361138858608066	0.0
54-55	1.9487122324954E-5	0.0	0.0	0.3872545905489275	0.0
56-57	2.5982829766605333E-5	0.0	0.0	0.44622911841168	0.0
58-59	2.5982829766605333E-5	0.0	0.0	0.5141092611769364	0.0
60-61	2.5982829766605333E-5	0.0	0.0	0.589959636973099	0.0
62-63	2.5982829766605333E-5	0.0	0.0	0.6681549631556978	0.0
64-65	2.5982829766605333E-5	0.0	0.0	0.7488836152405407	0.0
66-67	2.5982829766605333E-5	0.0	0.0	0.8432272701230845	0.0
68-69	2.5982829766605333E-5	0.0	0.0	0.9515237045902956	0.0
70-71	2.5982829766605333E-5	0.0	0.0	1.0728375367705758	0.0
72-73	2.5982829766605333E-5	0.0	0.0	1.2072791936904337	0.0
74-75	2.5982829766605333E-5	0.0	0.0	1.3454039167297074	0.0
76-77	2.5982829766605333E-5	0.0	0.0	1.4949675805737295	0.0
78-79	2.5982829766605333E-5	0.0	0.0	1.6640118710352638	0.0
80-81	2.5982829766605333E-5	0.0	0.0	1.8570772876160246	0.0
82-83	2.5982829766605333E-5	0.0	0.0	2.0661481273330145	0.0
84-85	2.5982829766605333E-5	0.0	0.0	2.279129382929878	0.0
86-87	2.5982829766605333E-5	0.0	0.0	2.509662040034084	0.0
88	2.5982829766605333E-5	0.0	0.0	2.6983688369214973	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12185	0.0	59.55984	1
GTATCAA	26795	0.0	49.240273	1
ATCAACG	34685	0.0	37.452686	3
TCAACGC	34900	0.0	37.22196	4
TATCAAC	35815	0.0	36.50722	2
CAACGCA	35980	0.0	36.10421	5
AACGCAG	37260	0.0	34.91437	6
ACGCAGA	42070	0.0	30.821955	7
CGCAGAG	42470	0.0	30.3878	8
GCAGAGT	46715	0.0	27.415182	9
GTACATG	25130	0.0	26.578678	1
TACATGG	25540	0.0	25.265987	2
ACATGGG	26080	0.0	24.18419	3
AGAGTAC	44270	0.0	23.196524	10-11
GAGTACT	32660	0.0	21.858614	12-13
AGTACTT	34920	0.0	21.796547	12-13
CAGAGTA	46380	0.0	21.188698	10-11
CATGGGG	16905	0.0	20.99029	4
GTGGTAT	5130	0.0	20.52402	1
GTACTTT	37340	0.0	18.911291	14-15
>>END_MODULE