FastQCFastQC Report
Wed 25 May 2016
SRR2431360_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431360_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299572
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA851672.581152949534061No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76440.23166641006772998No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41420.12553143256155647No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34920.10583190789593318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49250.044.4713251
GTATCAA88050.039.1802371
TCAACGC105950.030.519774
ATCAACG106800.030.4528983
TATCAAC118600.028.6515052
CAACGCA123250.026.2739935
AACGCAG128000.025.4458566
GTACATG196900.024.8965381
ACGCAGA129450.024.7251437
TACATGG192500.024.4397722
CGCAGAG130250.024.1763578
ACATGGG204950.021.9461253
GCAGAGT142400.021.1233989
CATGGGG115750.020.3833944
TATACTG13050.020.1683925
GCCCATA15750.019.6953531
AATCCGC16000.019.6810676
TCCGCTA16150.019.207258
GTATTAG14650.018.6076161
ATCCGCT17400.018.3676457
Produced by FastQC (version 0.11.5)