##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431360_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3299572
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.29852902133974	33.0	31.0	34.0	25.0	34.0
2	30.481196046032636	33.0	31.0	34.0	25.0	34.0
3	30.70383825538585	34.0	31.0	34.0	26.0	34.0
4	33.65923671312522	37.0	35.0	37.0	27.0	37.0
5	34.06739358922915	37.0	35.0	37.0	30.0	37.0
6	33.94818509794604	37.0	35.0	37.0	29.0	37.0
7	33.97709096816193	37.0	35.0	37.0	30.0	37.0
8	33.87063776756501	37.0	35.0	37.0	28.0	37.0
9	35.2116707863929	39.0	37.0	39.0	27.0	39.0
10-11	35.31066923225194	39.0	37.0	39.0	27.5	39.0
12-13	35.24458232764734	39.0	37.0	39.0	27.0	39.0
14-15	36.530255590725105	40.0	38.0	41.0	27.0	41.0
16-17	36.54728007147594	40.0	38.0	41.0	27.0	41.0
18-19	36.504843658510865	40.0	38.0	41.0	27.0	41.0
20-21	36.51241827727959	40.0	38.0	41.0	27.0	41.0
22-23	36.522149842464415	40.0	37.5	41.0	27.0	41.0
24-25	36.5389833893608	40.0	37.0	41.0	27.0	41.0
26-27	36.54203014815255	40.0	37.0	41.0	27.5	41.0
28-29	36.51126176364692	40.0	37.0	41.0	28.0	41.0
30-31	36.43928970181587	40.0	37.0	41.0	27.5	41.0
32-33	36.34626657639233	40.0	37.0	41.0	27.0	41.0
34-35	36.263443410236235	40.0	36.5	41.0	27.0	41.0
36-37	36.14600333012888	40.0	36.0	41.0	27.0	41.0
38-39	36.02591714925451	40.0	36.0	41.0	26.0	41.0
40-41	35.91157110679809	40.0	36.0	41.0	26.0	41.0
42-43	35.76512317355099	40.0	35.0	41.0	25.0	41.0
44-45	35.613287268773036	40.0	35.0	41.0	25.0	41.0
46-47	35.466151973649914	40.0	35.0	41.0	25.0	41.0
48-49	35.316741534962716	40.0	35.0	41.0	25.0	41.0
50-51	35.152299752816425	39.0	35.0	41.0	25.0	41.0
52-53	34.944343690636245	39.0	35.0	41.0	25.0	41.0
54-55	34.76386891996901	39.0	34.0	41.0	25.0	41.0
56-57	34.55086826412638	38.5	34.0	41.0	25.0	41.0
58-59	34.29499447201031	38.0	34.0	40.0	25.0	41.0
60-61	34.08694173062446	38.0	34.0	40.0	24.5	41.0
62-63	33.90008052559544	37.0	33.0	40.0	24.0	41.0
64-65	33.632744792354885	37.0	33.0	40.0	23.0	41.0
66-67	33.33257692209777	36.0	33.0	40.0	22.5	41.0
68-69	33.030224374555246	36.0	33.0	39.0	22.0	41.0
70-71	32.80102843641539	35.0	33.0	39.0	21.5	41.0
72-73	32.5680135181169	35.0	33.0	39.0	20.5	41.0
74-75	32.251883577627645	35.0	32.5	38.0	19.5	40.0
76-77	31.939231815520316	35.0	32.0	37.0	18.5	39.5
78-79	31.635687446735517	35.0	32.0	37.0	17.5	39.0
80-81	31.333719646063187	35.0	32.0	36.0	14.0	39.0
82-83	31.04115473158337	35.0	31.5	36.0	10.5	38.5
84-85	30.781406194500377	35.0	31.0	35.5	7.5	37.0
86-87	30.543370928108253	35.0	31.0	35.0	4.5	37.0
88-89	30.319057592924175	35.0	31.0	35.0	2.0	36.5
90-91	30.126559899283908	35.0	31.0	35.0	2.0	36.0
92-93	29.93255125210179	35.0	31.0	35.0	2.0	36.0
94-95	29.71775475728367	35.0	30.5	35.0	2.0	36.0
96-97	29.539721515396543	34.5	30.0	35.0	2.0	35.0
98-99	29.41931620222259	34.5	30.0	35.0	2.0	35.0
100	29.36813350337559	35.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5096532427939486
1101	2	0.528212135477478
1101	3	0.4736261981597991
1101	4	0.4657821019296975
1101	5	0.3082556443359863
1101	6	0.48097119617362694
1101	7	0.4444352044773865
1101	8	0.5129393573611551
1101	9	0.6546806138816805
1101	10-11	0.5504465793397344
1101	12-13	0.5529244825024264
1101	14-15	0.6300945988482027
1101	16-17	0.6303613114388824
1101	18-19	0.6047711426474436
1101	20-21	0.5933899850322888
1101	22-23	0.5508411887067126
1101	24-25	0.628590942075526
1101	26-27	0.6042997433210502
1101	28-29	0.7182798153894012
1101	30-31	0.7262458038347361
1101	32-33	0.6896214688800129
1101	34-35	0.7228999509612777
1101	36-37	0.7992684278244369
1101	38-39	0.733346650727789
1101	40-41	0.7504047626175705
1101	42-43	0.7685267220412655
1101	44-45	0.7441661426798092
1101	46-47	0.7686909663864583
1101	48-49	0.8392299214708459
1101	50-51	0.7828492538007197
1101	52-53	0.8418484675849811
1101	54-55	0.8532146719011919
1101	56-57	0.8168451588016765
1101	58-59	0.9102491411047922
1101	60-61	0.913717180654082
1101	62-63	0.8619362416592793
1101	64-65	0.8723149305786748
1101	66-67	0.8095367615222457
1101	68-69	0.8222955709306987
1101	70-71	0.7895065894910545
1101	72-73	0.7879878235548219
1101	74-75	0.7954180944092926
1101	76-77	0.819716727334459
1101	78-79	0.8499823373441373
1101	80-81	0.7715307220935941
1101	82-83	0.779533173332954
1101	84-85	0.8063993120598738
1101	86-87	0.8390592391920464
1101	88-89	0.845845765815362
1101	90-91	0.8349609596009948
1101	92-93	0.8523951992488996
1101	94-95	0.8840116408031236
1101	96-97	0.8628740053172699
1101	98-99	0.8547521139627143
1101	100	0.8333885017580833
1102	1	0.14758563823332693
1102	2	0.2689035380289475
1102	3	0.292910703552689
1102	4	0.2436828615978328
1102	5	0.31657974584692283
1102	6	0.36447750411830526
1102	7	0.26027300914816465
1102	8	0.31876453726288645
1102	9	0.37618069864026893
1102	10-11	0.36107569068369827
1102	12-13	0.3958020632945036
1102	14-15	0.43991086211914876
1102	16-17	0.47851924773246424
1102	18-19	0.41129485026630164
1102	20-21	0.34285853147305545
1102	22-23	0.22678278216023529
1102	24-25	0.3611090861929753
1102	26-27	0.30059047167691233
1102	28-29	0.40748274861234535
1102	30-31	0.402965284393737
1102	32-33	0.40289550793643514
1102	34-35	0.4683734204060457
1102	36-37	0.4866715740080849
1102	38-39	0.5057931871088144
1102	40-41	0.5039409629703755
1102	42-43	0.4070593662813593
1102	44-45	0.4237275683858357
1102	46-47	0.4570439487354818
1102	48-49	0.396829956132855
1102	50-51	0.369896931502268
1102	52-53	0.3797720815075536
1102	54-55	0.3722453521916407
1102	56-57	0.4381769623900311
1102	58-59	0.3786127005409128
1102	60-61	0.3889140140248486
1102	62-63	0.3506926650956359
1102	64-65	0.4220362981374919
1102	66-67	0.4635621343049863
1102	68-69	0.40187840158392163
1102	70-71	0.3414080979976788
1102	72-73	0.29173933393779095
1102	74-75	0.3768696523632684
1102	76-77	0.34936936167571986
1102	78-79	0.32656393291807007
1102	80-81	0.32777390531847317
1102	82-83	0.42269092824001575
1102	84-85	0.3701919001294982
1102	86-87	0.3965942602870953
1102	88-89	0.361750440308672
1102	90-91	0.36820553773947395
1102	92-93	0.34176835914072967
1102	94-95	0.36010451908670404
1102	96-97	0.3622458234397392
1102	98-99	0.3714829783660534
1102	100	0.3938006297645771
1103	1	0.1469483347917908
1103	2	0.16225992029636416
1103	3	0.26089033184914
1103	4	0.268726127154153
1103	5	0.23112575765576082
1103	6	0.23798772302431814
1103	7	0.22193009393126317
1103	8	0.25089331799102155
1103	9	0.22181297384461374
1103	10-11	0.2776792954856475
1103	12-13	0.26442653317330667
1103	14-15	0.3012910411302414
1103	16-17	0.2985227537000199
1103	18-19	0.2919996455798284
1103	20-21	0.2531685207652572
1103	22-23	0.2709453949872085
1103	24-25	0.27592476733609317
1103	26-27	0.3843584497434591
1103	28-29	0.3990268535688202
1103	30-31	0.3649384919156944
1103	32-33	0.321684241845567
1103	34-35	0.390568116163756
1103	36-37	0.3515238898944304
1103	38-39	0.3405430534942724
1103	40-41	0.30850483471567713
1103	42-43	0.3038901481973113
1103	44-45	0.30589790871670175
1103	46-47	0.26715842554192193
1103	48-49	0.2607352348457397
1103	50-51	0.23664323299933443
1103	52-53	0.19773656253340732
1103	54-55	0.27150994643601933
1103	56-57	0.24718682870271635
1103	58-59	0.31963843963144
1103	60-61	0.2641216678421827
1103	62-63	0.2546918684219719
1103	64-65	0.3340266184370435
1103	66-67	0.31597984544858804
1103	68-69	0.35521010010052834
1103	70-71	0.30522653058438465
1103	72-73	0.31269212654165557
1103	74-75	0.34861193987799055
1103	76-77	0.3222295701152902
1103	78-79	0.316000131451851
1103	80-81	0.3431399106726367
1103	82-83	0.3328070289190528
1103	84-85	0.2568605450220147
1103	86-87	0.21273532317116306
1103	88-89	0.23825080319539182
1103	90-91	0.14074914870148092
1103	92-93	0.2339789794982181
1103	94-95	0.15335206195894102
1103	96-97	0.15630492548078934
1103	98-99	0.2139108338328768
1103	100	0.18973015795327441
1104	1	0.1680707696718322
1104	2	0.11991150456683997
1104	3	0.13502797735780092
1104	4	0.0911892766179605
1104	5	0.14575253443774017
1104	6	0.044254414240292306
1104	7	0.12963651985012348
1104	8	0.24334320534191534
1104	9	0.2425164247026217
1104	10-11	0.25692235730567603
1104	12-13	0.21824425935182035
1104	14-15	0.2688671808473586
1104	16-17	0.267733771128313
1104	18-19	0.2472124994061744
1104	20-21	0.30144241459772303
1104	22-23	0.2676108376170845
1104	24-25	0.36361106007838373
1104	26-27	0.3725672381904701
1104	28-29	0.4266933422872867
1104	30-31	0.45789671454569714
1104	32-33	0.4112557055364192
1104	34-35	0.45237863115408317
1104	36-37	0.42746401529476685
1104	38-39	0.49576943344145974
1104	40-41	0.45290560356164633
1104	42-43	0.41965419916250113
1104	44-45	0.4215868288522415
1104	46-47	0.37443886437111473
1104	48-49	0.37259089690735436
1104	50-51	0.28773108283984783
1104	52-53	0.3557332257474002
1104	54-55	0.3625962413726569
1104	56-57	0.33598109760474415
1104	58-59	0.3144595553975549
1104	60-61	0.31435144903446144
1104	62-63	0.2852851593974606
1104	64-65	0.308133268728362
1104	66-67	0.36866627190917
1104	68-69	0.3358041025259695
1104	70-71	0.33584892243456466
1104	72-73	0.341184497226628
1104	74-75	0.34913931287217537
1104	76-77	0.283319129150037
1104	78-79	0.3131354310614256
1104	80-81	0.31901815707021086
1104	82-83	0.2993139364981019
1104	84-85	0.2712200051977902
1104	86-87	0.3406820974570657
1104	88-89	0.3101771079517519
1104	90-91	0.24520037392116478
1104	92-93	0.2502861839319088
1104	94-95	0.22522273647178537
1104	96-97	0.2700073144783097
1104	98-99	0.33600147495728905
1104	100	0.31140421512779426
1105	1	0.13910906212148078
1105	2	0.20753541038159895
1105	3	0.15977652674057907
1105	4	0.2153695603079484
1105	5	0.1378042643644335
1105	6	0.12051523765395444
1105	7	0.19081906039908603
1105	8	0.13817646074598855
1105	9	0.19668732255074417
1105	10-11	0.25759462595359395
1105	12-13	0.21559801152577762
1105	14-15	0.23666754666861323
1105	16-17	0.1648337554051409
1105	18-19	0.18561430750244767
1105	20-21	0.1725486977562838
1105	22-23	0.20215935253247608
1105	24-25	0.17451612781505332
1105	26-27	0.20042895871758049
1105	28-29	0.2721481093268423
1105	30-31	0.25056985311766766
1105	32-33	0.2791746130985757
1105	34-35	0.29781245684932145
1105	36-37	0.2489772471346754
1105	38-39	0.2581473127904843
1105	40-41	0.21076126826394415
1105	42-43	0.2284940555529431
1105	44-45	0.18123460917273349
1105	46-47	0.21819017320513012
1105	48-49	0.13859231542595296
1105	50-51	0.1521709808383349
1105	52-53	0.2090965258011579
1105	54-55	0.1548862563336968
1105	56-57	0.1386861700103026
1105	58-59	0.141508082328464
1105	60-61	0.16295102238031944
1105	62-63	0.267852691799888
1105	64-65	0.18657219793220037
1105	66-67	0.19751094824181337
1105	68-69	0.1259399054274155
1105	70-71	0.2004896513581258
1105	72-73	0.1511257481732855
1105	74-75	0.14056189272219655
1105	76-77	0.1961119904174069
1105	78-79	0.11145243357919199
1105	80-81	0.19006976958805666
1105	82-83	0.1943007331521578
1105	84-85	0.2230296806481462
1105	86-87	0.2729072388797995
1105	88-89	0.2684670995683476
1105	90-91	0.2807631536683459
1105	92-93	0.2417016917869681
1105	94-95	0.3016052599930319
1105	96-97	0.27221113349291315
1105	98-99	0.26834342445226156
1105	100	0.3033486635774132
1106	1	0.17177478159280923
1106	2	0.1636258387276719
1106	3	0.20658313492237568
1106	4	0.1898941519814059
1106	5	0.12398921300506771
1106	6	0.14684247560487762
1106	7	0.1740458275255392
1106	8	0.21407047394909995
1106	9	0.24411176164444726
1106	10-11	0.24661144769051901
1106	12-13	0.23298630884386995
1106	14-15	0.27117644015437037
1106	16-17	0.26391817587263944
1106	18-19	0.20750641366048228
1106	20-21	0.2697689029029604
1106	22-23	0.20732493267077245
1106	24-25	0.3131113964693242
1106	26-27	0.32891006313981563
1106	28-29	0.32525721476034875
1106	30-31	0.36552266970451797
1106	32-33	0.2974357813474455
1106	34-35	0.314591263043944
1106	36-37	0.30409541365742854
1106	38-39	0.24666655407570204
1106	40-41	0.2161544054863782
1106	42-43	0.25980509452499234
1106	44-45	0.2253296956844153
1106	46-47	0.26223934039749963
1106	48-49	0.29075575347042104
1106	50-51	0.2601565200894598
1106	52-53	0.22103455935577898
1106	54-55	0.24614076648320804
1106	56-57	0.26266397042240186
1106	58-59	0.23869288880901962
1106	60-61	0.25778231229720916
1106	62-63	0.2749450255755903
1106	64-65	0.3328238164165711
1106	66-67	0.3486592745088757
1106	68-69	0.2812254937076091
1106	70-71	0.3195126885596409
1106	72-73	0.32389419849116763
1106	74-75	0.3492597064456575
1106	76-77	0.31184892885482185
1106	78-79	0.32554722123161994
1106	80-81	0.33375067353227195
1106	82-83	0.33457942747649483
1106	84-85	0.24631222944903186
1106	86-87	0.27211615037788306
1106	88-89	0.3282068416285888
1106	90-91	0.31644187917097355
1106	92-93	0.25829381191869416
1106	94-95	0.2893299799336475
1106	96-97	0.2948235106274346
1106	98-99	0.344748850443807
1106	100	0.40221445235177455
1107	1	0.03660002644603111
1107	2	0.08024947933525794
1107	3	0.12228017438581418
1107	4	-0.026431181498637102
1107	5	0.10002306353160861
1107	6	0.011073875221839558
1107	7	0.05016590019771172
1107	8	0.07426515419027879
1107	9	0.01027220187472011
1107	10-11	0.07482488949165855
1107	12-13	0.12272822353659762
1107	14-15	0.05620481075621342
1107	16-17	0.05305059672924983
1107	18-19	0.06006129807306593
1107	20-21	0.033395454077250974
1107	22-23	0.06051186384918594
1107	24-25	0.0485514126899389
1107	26-27	0.07944004787924541
1107	28-29	0.07604633958462159
1107	30-31	0.043491572529987366
1107	32-33	0.10208135695632592
1107	34-35	0.05806035906314122
1107	36-37	0.05042037004083255
1107	38-39	0.03133801272377923
1107	40-41	0.004321695759159638
1107	42-43	5.9942630649345574E-5
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2216	88-89	-1.1592873404500459
2216	90-91	-1.1552034927264074
2216	92-93	-1.1824768718899001
2216	94-95	-1.1595543227546727
2216	96-97	-1.2963544535152245
2216	98-99	-1.3318163039020732
2216	100	-1.3363825423451736
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77640.0
3	19641.0
4	17662.0
5	18186.0
6	11053.0
7	8753.0
8	8977.0
9	8589.0
10	8339.0
11	8863.0
12	10269.0
13	11119.0
14	11294.0
15	10984.0
16	10631.0
17	10028.0
18	9759.0
19	9146.0
20	8882.0
21	9535.0
22	12205.0
23	24567.0
24	44962.0
25	45328.0
26	48933.0
27	33689.0
28	33197.0
29	33351.0
30	39015.0
31	46909.0
32	62625.0
33	83444.0
34	119195.0
35	213013.0
36	362038.0
37	639470.0
38	801944.0
39	360293.0
40	6044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61335401891343	23.796983901797844	13.035825068850068	21.553837010438663
2	14.857805800267428	25.311343410599918	37.491195827822516	22.339654961310135
3	16.649219959437165	32.55437371877322	28.442355553993064	22.35405076779655
4	11.516251198640308	21.854137445705078	37.38378795795334	29.245823397701276
5	12.320719172062317	42.57749186864236	32.00363562304444	13.098153336250885
6	26.537744895398557	42.49472355808572	17.883198184491807	13.084333362023923
7	23.683404999193836	38.015112263045026	22.259311207635417	16.042171530125728
8	22.943733308441217	39.307249546304796	20.322241793784162	17.42677535146983
9	24.508178636501945	21.5116990930945	20.20980296838499	33.770319302018564
10-11	22.41622549833736	31.808170877919924	26.369026043377747	19.40657758036497
12-13	22.76648304689214	30.547219457553886	26.53156227534965	20.154735220204316
14-15	21.208387027165948	30.5424461111926	24.461293767797763	23.787873093843686
16-17	20.675242122311623	33.621102979416726	24.81255144606634	20.891103452205318
18-19	20.47141265594447	32.362939799464904	26.675929484187645	20.489718060402986
20-21	23.15095715444306	30.891809604397174	25.385959148641096	20.571274092518664
22-23	22.17351220097637	31.599325609503293	25.769554354322317	20.45760783519802
24-25	21.565812172002914	31.008400483456644	25.930908614814285	21.494878729726157
26-27	21.559947775044762	31.662788385887623	25.808074501783867	20.969189337283744
28-29	21.07436964551766	31.38010020693593	26.42301789444207	21.122512253104343
30-31	21.950240819112295	31.556683715342476	25.44342114674267	21.04965431880256
32-33	21.000102437528263	31.63908834236683	26.37692100672451	20.983888213380403
34-35	21.705102964869383	30.615228278091827	26.25458695855099	21.425081798487806
36-37	21.658566626216977	31.495994025891843	25.798724804307955	21.046714543583228
38-39	21.220903802068875	31.222397935247358	26.16231741571331	21.394380846970456
40-41	21.77994600511824	30.95205984291296	25.830577420344213	21.437416731624587
42-43	21.10400985339917	31.446760367708297	26.45779513221715	20.991434646675387
44-45	21.453024816551967	30.80443463576488	26.05748563753117	21.685054910151983
46-47	21.558356659591002	31.302575000636445	25.995613976600602	21.14345436317195
48-49	21.082400990189033	31.085789308431515	26.190502889465666	21.64130681191379
50-51	21.641200737550204	31.136432240302682	25.585394105659766	21.636972916487352
52-53	21.16065356355309	31.371508183485613	26.063380341450348	21.404457911510946
54-55	21.38878921266152	30.933481675805226	25.953729756465382	21.723999355067868
56-57	21.47417907534674	31.335609588152646	25.69080171610136	21.499409620399252
58-59	21.364240574232053	30.699254327530966	26.216915405998108	21.719589692238873
60-61	21.91175097861177	31.21315431213503	25.393020670559697	21.4820740386935
62-63	21.031621676993257	31.421802585305002	26.128616075054584	21.417959662647153
64-65	21.64599251751326	30.592987604602595	25.910091658871465	21.85092821901268
66-67	21.459510506211107	31.13778090006825	25.733413303301155	21.669295290419484
68-69	21.41286809319512	31.16375396566585	26.052742598130905	21.370635343008125
70-71	21.92055515078925	31.011264491273415	25.57104375961488	21.49713659832245
72-73	21.3780453949785	31.32012273106936	26.064622926852334	21.237208947099806
74-75	21.564054368263523	30.739426204368325	25.91593697606841	21.780582451299743
76-77	21.65033828629895	31.106291967564275	25.512233101747743	21.73113664438903
78-79	21.416126091505202	30.927480897522468	26.12335781731691	21.53303519365542
80-81	21.61046947907183	31.166663433924157	25.745217864620017	21.47764922238399
82-83	21.14449207525166	31.536320582524652	26.23179364210805	21.087393700115637
84-85	21.387757464760004	30.56412026657546	25.946450027253686	22.101672241410846
86-87	21.798473510557486	31.219129140000636	25.684919207469303	21.297478141972583
88-89	21.116502390009046	30.99398402377113	26.0988714986932	21.790642087526628
90-91	21.646644818772902	30.749400132566386	25.56071731324855	22.043237735412166
92-93	21.188876201791025	31.212857089818847	26.14930584232017	21.44896086606996
94-95	21.330845686328264	30.419667686365536	26.163027255464343	22.086459371841855
96-97	21.416330416785183	31.322208737036505	25.656102103784946	21.605358742393367
98-99	20.7937401291653	31.1947320391345	26.14154659518902	21.869981236511187
100	21.29784480537783	31.010101622381775	25.55768047595523	22.134373096285163
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	78253.0
1	53497.0
2	21633.0
3	10876.5
4	5481.0
5	3234.5
6	2508.0
7	2590.0
8	3187.5
9	3283.5
10	2458.5
11	1655.0
12	1462.5
13	1476.5
14	1493.5
15	1595.0
16	1793.0
17	2083.0
18	2520.5
19	3133.0
20	4211.0
21	5604.0
22	7175.0
23	9726.5
24	13267.0
25	17449.0
26	22692.5
27	28778.5
28	36260.0
29	44636.5
30	51254.0
31	57107.5
32	62779.0
33	67592.0
34	73137.0
35	78577.0
36	83114.5
37	88336.5
38	94544.5
39	99866.0
40	104724.5
41	106966.5
42	105674.5
43	105239.5
44	103630.5
45	101266.5
46	101070.0
47	99428.0
48	99262.5
49	103684.0
50	107747.5
51	104890.0
52	100252.0
53	96929.5
54	93857.5
55	92651.0
56	83677.5
57	71827.0
58	65949.0
59	62124.0
60	55408.5
61	45975.5
62	37883.5
63	30349.5
64	23734.5
65	19136.5
66	15467.5
67	11964.0
68	9028.5
69	6692.0
70	5033.0
71	4151.5
72	3190.5
73	2244.5
74	1681.0
75	1354.0
76	1018.0
77	731.5
78	542.0
79	419.0
80	292.5
81	182.5
82	149.0
83	123.0
84	107.5
85	91.5
86	78.0
87	69.5
88	52.0
89	49.5
90	46.0
91	29.0
92	22.5
93	23.0
94	23.0
95	20.5
96	17.0
97	19.5
98	19.0
99	19.0
100	48.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.333765712643943E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	1.5153480512017922E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.0607436358412545E-4
84-85	5.606787789446631E-4
86-87	0.0012274319214734515
88-89	0.003257998310083853
90-91	0.0028185473752353335
92-93	0.0021063337911704913
94-95	0.0020760268301464554
96-97	0.0020154129080983837
98-99	0.002394249920898832
100	0.003091310024451656
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3299572.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.18096228767223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.78577087717846	38.27607718919367
2	13.501935479993454	13.820841012242575
3	4.803615486350131	7.375609891540931
4	2.303983423253018	4.7168035478773875
5	1.2974781971866978	3.320309133964466
6	0.7833011923034058	2.4054065269901588
7	0.5259696863432581	1.8843744276835162
8	0.3653935313591493	1.4960954038921603
9	0.2633701976786902	1.2131586139580839
>10	1.2259611820648462	10.939483945537413
>50	0.07962727746281931	2.8261185726662266
>100	0.05749249688305619	5.7334839178868835
>500	0.003968593400692993	1.3909231172805891
>1k	0.0020139130677878187	1.7876671027447295
>5k	5.923273728787702E-5	0.23173462442987236
>10k+	5.923273728787702E-5	2.581912972111321
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	85167	2.581152949534061	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7644	0.23166641006772998	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4142	0.12553143256155647	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3492	0.10583190789593318	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	3.0306961024035844E-5	0.0	0.0	0.0
14-15	0.0	3.0306961024035844E-5	0.0	0.0	3.0306961024035844E-5
16-17	0.0	3.0306961024035844E-5	0.0	1.5153480512017922E-5	3.0306961024035844E-5
18-19	0.0	3.0306961024035844E-5	1.5153480512017922E-5	1.0607436358412545E-4	3.0306961024035844E-5
20-21	0.0	3.0306961024035844E-5	3.0306961024035844E-5	3.78837012800448E-4	3.0306961024035844E-5
22-23	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.0010607436358412545	3.0306961024035844E-5
24-25	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.002166947713218563	3.0306961024035844E-5
26-27	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.0034246865957160504	3.0306961024035844E-5
28-29	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.006955447555016226	3.0306961024035844E-5
30-31	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.015456550122258281	3.0306961024035844E-5
32-33	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.02770056237596876	3.0306961024035844E-5
34-35	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.04441485138072453	3.0306961024035844E-5
36-37	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.06719053259028747	3.0306961024035844E-5
38-39	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.10020996662597452	3.0306961024035844E-5
40-41	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.13707838471171413	3.0306961024035844E-5
42-43	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.17156770635706692	3.0306961024035844E-5
44-45	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.21176989015545045	3.0306961024035844E-5
46-47	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.2569272620812639	3.0306961024035844E-5
48-49	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.30763080787447583	3.0306961024035844E-5
50-51	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.3663656983390573	3.0306961024035844E-5
52-53	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.42516120272568686	3.0306961024035844E-5
54-55	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.4850477577091816	3.0306961024035844E-5
56-57	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.5530717317276301	3.0306961024035844E-5
58-59	0.0	3.0306961024035844E-5	3.0306961024035844E-5	0.6290058225733519	3.0306961024035844E-5
60-61	0.0	4.546044153605377E-5	3.0306961024035844E-5	0.7162444098810391	3.0306961024035844E-5
62-63	0.0	6.061392204807169E-5	3.0306961024035844E-5	0.8066652280962501	3.0306961024035844E-5
64-65	0.0	6.061392204807169E-5	3.0306961024035844E-5	0.8994802962323598	3.0306961024035844E-5
66-67	0.0	6.061392204807169E-5	3.0306961024035844E-5	1.0006297786500795	3.0306961024035844E-5
68-69	0.0	6.061392204807169E-5	3.0306961024035844E-5	1.121206023084206	3.0306961024035844E-5
70-71	0.0	6.061392204807169E-5	3.0306961024035844E-5	1.2574358128872472	3.0306961024035844E-5
72-73	0.0	6.061392204807169E-5	3.0306961024035844E-5	1.4064248332814073	3.0306961024035844E-5
74-75	0.0	6.061392204807169E-5	3.0306961024035844E-5	1.561475245880375	3.0306961024035844E-5
76-77	0.0	6.061392204807169E-5	3.0306961024035844E-5	1.7202079542437625	3.0306961024035844E-5
78-79	0.0	6.061392204807169E-5	3.0306961024035844E-5	1.9010495906741844	3.0306961024035844E-5
80-81	0.0	6.061392204807169E-5	3.0306961024035844E-5	2.111334439739457	3.0306961024035844E-5
82-83	0.0	6.061392204807169E-5	3.0306961024035844E-5	2.3398792328217115	3.0306961024035844E-5
84-85	0.0	6.061392204807169E-5	3.0306961024035844E-5	2.572727614369379	3.0306961024035844E-5
86-87	0.0	6.061392204807169E-5	3.0306961024035844E-5	2.8186686030794297	3.0306961024035844E-5
88	0.0	6.061392204807169E-5	3.0306961024035844E-5	3.010845042932841	3.0306961024035844E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4925	0.0	44.471325	1
GTATCAA	8805	0.0	39.180237	1
TCAACGC	10595	0.0	30.51977	4
ATCAACG	10680	0.0	30.452898	3
TATCAAC	11860	0.0	28.651505	2
CAACGCA	12325	0.0	26.273993	5
AACGCAG	12800	0.0	25.445856	6
GTACATG	19690	0.0	24.896538	1
ACGCAGA	12945	0.0	24.725143	7
TACATGG	19250	0.0	24.439772	2
CGCAGAG	13025	0.0	24.176357	8
ACATGGG	20495	0.0	21.946125	3
GCAGAGT	14240	0.0	21.123398	9
CATGGGG	11575	0.0	20.383394	4
TATACTG	1305	0.0	20.168392	5
GCCCATA	1575	0.0	19.695353	1
AATCCGC	1600	0.0	19.681067	6
TCCGCTA	1615	0.0	19.20725	8
GTATTAG	1465	0.0	18.607616	1
ATCCGCT	1740	0.0	18.367645	7
>>END_MODULE