FastQCFastQC Report
Wed 25 May 2016
SRR2431360_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431360_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299572
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT529171.6037534565089047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342071.036710215749194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319500.9683074047179453No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133850.4056586733067198No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92220.27949079456365855No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72000.21821011937305806No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58710.17793216817211446No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46120.13977570424285332No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46020.13947263463261297No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35800.10849892046604832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105650.063.708991
GTATCAA230600.050.040241
ATCAACG322800.035.5409133
TATCAAC323250.035.5350532
TCAACGC325450.035.2659534
CAACGCA338850.033.8425835
AACGCAG347050.033.110676
ACGCAGA375350.030.4765157
CGCAGAG378300.030.114628
GTGGTAT34000.029.9990841
TGGTATC34250.028.4056052
GCAGAGT399550.028.2659639
GTACATG179600.025.5690981
TACATGG176000.025.4492232
AGAGTAC388400.024.12831910-11
ACATGGG178550.023.9538783
AGTACTT306550.023.61024912-13
GAGTACT303050.022.68102612-13
CAGAGTA394800.022.45151710-11
CATGGGG106000.021.1498264
Produced by FastQC (version 0.11.5)