##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431360_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3299572
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74973269260377	34.0	31.0	34.0	31.0	34.0
2	32.86893754705156	34.0	31.0	34.0	31.0	34.0
3	32.9372424665987	34.0	31.0	34.0	31.0	34.0
4	36.334488836733975	37.0	37.0	37.0	35.0	37.0
5	36.30268956094912	37.0	37.0	37.0	35.0	37.0
6	36.3459933591387	37.0	37.0	37.0	35.0	37.0
7	36.2082967124221	37.0	37.0	37.0	35.0	37.0
8	36.26234493443392	37.0	37.0	37.0	35.0	37.0
9	38.07110285818888	39.0	39.0	39.0	35.0	39.0
10-11	38.06550304100047	39.0	39.0	39.0	35.0	39.0
12-13	38.03530730652339	39.0	39.0	39.0	35.0	39.0
14-15	39.44764805859669	41.0	39.0	41.0	36.0	41.0
16-17	39.47666515535954	41.0	39.0	41.0	36.5	41.0
18-19	39.587017194957404	41.0	40.0	41.0	37.0	41.0
20-21	39.586146779036795	41.0	40.0	41.0	37.0	41.0
22-23	39.487085446233635	41.0	40.0	41.0	36.0	41.0
24-25	39.43226757894661	41.0	39.5	41.0	36.0	41.0
26-27	39.3111680848304	41.0	39.0	41.0	35.5	41.0
28-29	39.194229433393176	41.0	39.0	41.0	35.0	41.0
30-31	38.92332566163127	41.0	38.5	41.0	35.0	41.0
32-33	38.61015959039536	41.0	38.0	41.0	35.0	41.0
34-35	38.46318977127943	41.0	38.0	41.0	35.0	41.0
36-37	38.31335367132465	40.5	38.0	41.0	35.0	41.0
38-39	38.16491669222553	40.0	38.0	41.0	35.0	41.0
40-41	38.018186298101696	40.0	37.5	41.0	34.0	41.0
42-43	37.81580565600629	40.0	37.0	41.0	34.0	41.0
44-45	37.50638249445686	40.0	37.0	41.0	33.0	41.0
46-47	37.048842092247114	40.0	37.0	41.0	32.5	41.0
48-49	36.663541665403876	40.0	36.0	41.0	31.0	41.0
50-51	36.46730015286831	40.0	36.0	41.0	31.0	41.0
52-53	36.325741035503995	40.0	35.0	41.0	31.0	41.0
54-55	36.156839887112625	40.0	35.0	41.0	30.0	41.0
56-57	35.962857910056215	39.0	35.0	41.0	30.0	41.0
58-59	35.74714296278427	39.0	35.0	41.0	30.0	41.0
60-61	35.53135149043573	39.0	35.0	41.0	29.0	41.0
62-63	35.289047791652976	38.0	35.0	41.0	29.0	41.0
64-65	35.014077280326056	37.5	35.0	41.0	28.5	41.0
66-67	34.72885149952782	37.0	35.0	40.0	28.0	41.0
68-69	34.463532391473805	36.5	35.0	40.0	28.0	41.0
70-71	34.22340200486609	36.0	35.0	39.0	28.0	41.0
72-73	33.978069125328986	36.0	35.0	39.0	28.0	41.0
74-75	33.709115606508966	35.0	35.0	39.0	28.0	41.0
76-77	33.42646500818894	35.0	35.0	37.5	27.0	40.0
78-79	33.14738093304223	35.0	34.0	37.0	27.0	39.0
80-81	32.88069922402057	35.0	34.0	36.5	27.0	39.0
82-83	32.60311291888766	35.0	34.0	36.0	26.0	39.0
84-85	32.37380211736553	35.0	34.0	36.0	26.0	37.0
86-87	32.17600782768189	35.0	34.0	35.5	26.0	37.0
88-89	31.976450582075493	35.0	34.0	35.0	25.0	37.0
90-91	31.792582492517212	35.0	34.0	35.0	25.0	36.0
92-93	31.65514875868749	35.0	34.0	35.0	24.0	36.0
94-95	31.526198397852813	35.0	34.0	35.0	24.0	36.0
96-97	31.39415703006329	35.0	34.0	35.0	23.5	36.0
98-99	31.275367684051144	35.0	34.0	35.0	22.5	35.5
100	31.166185796218418	35.0	34.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24652168980268385
1101	2	0.2408759365574369
1101	3	0.22390242947062688
1101	4	0.15664514961306963
1101	5	0.1880085347415914
1101	6	0.19132975464189883
1101	7	0.2310760663031175
1101	8	0.23501065247423725
1101	9	0.2950864628827361
1101	10-11	0.2937803598847566
1101	12-13	0.2967953688338625
1101	14-15	0.39227252040774374
1101	16-17	0.41506758701734725
1101	18-19	0.3803211955074133
1101	20-21	0.3638137140596882
1101	22-23	0.39876047736798625
1101	24-25	0.40037249069173697
1101	26-27	0.4043213458763404
1101	28-29	0.46176949595778893
1101	30-31	0.47791646202394844
1101	32-33	0.48248488483644536
1101	34-35	0.4757306975153668
1101	36-37	0.4680325866609678
1101	38-39	0.4888243771825316
1101	40-41	0.4985459985217702
1101	42-43	0.5769887209804665
1101	44-45	0.5114229881978432
1101	46-47	0.4912230079399933
1101	48-49	0.4998813369915496
1101	50-51	0.563017919447681
1101	52-53	0.5012000725862649
1101	54-55	0.541815423385593
1101	56-57	0.5648745319791644
1101	58-59	0.5904759106782578
1101	60-61	0.5666390170014495
1101	62-63	0.5722792838154263
1101	64-65	0.6291056014194965
1101	66-67	0.5981982384993998
1101	68-69	0.5677317362135881
1101	70-71	0.4949504949506789
1101	72-73	0.4877336671131687
1101	74-75	0.49002072217113835
1101	76-77	0.5126103721311566
1101	78-79	0.49319564367367974
1101	80-81	0.5120498459061977
1101	82-83	0.5462420473064569
1101	84-85	0.4699771407905118
1101	86-87	0.4906966767158991
1101	88-89	0.45718035561453974
1101	90-91	0.4304193007461308
1101	92-93	0.48032206085555984
1101	94-95	0.5324415862425838
1101	96-97	0.5310123302881635
1101	98-99	0.5735904495828805
1101	100	0.6096900442536857
1102	1	0.16233974052281752
1102	2	0.18503695744802684
1102	3	0.2202119167170551
1102	4	0.14293152603126913
1102	5	0.13884872911832957
1102	6	0.15996809345021745
1102	7	0.21181924410647213
1102	8	0.2107188708718084
1102	9	0.2842537836638215
1102	10-11	0.2601864174892725
1102	12-13	0.24441163546926958
1102	14-15	0.3215968384669665
1102	16-17	0.3257828312976869
1102	18-19	0.322556217365495
1102	20-21	0.3242520884492137
1102	22-23	0.3533704992651039
1102	24-25	0.36883981749227246
1102	26-27	0.400035314204068
1102	28-29	0.35578426309566424
1102	30-31	0.30139882694889053
1102	32-33	0.35526808172532043
1102	34-35	0.3214390903831159
1102	36-37	0.30155894528945737
1102	38-39	0.3337099490656499
1102	40-41	0.37474077898986735
1102	42-43	0.4327611872395636
1102	44-45	0.4826461629062564
1102	46-47	0.39772459666720295
1102	48-49	0.3820845624408875
1102	50-51	0.34129713660937
1102	52-53	0.2925226979724229
1102	54-55	0.2793320711200806
1102	56-57	0.24162038386148055
1102	58-59	0.2544731687745809
1102	60-61	0.22364620111214606
1102	62-63	0.19538896527259197
1102	64-65	0.23977475612309718
1102	66-67	0.24735051647081718
1102	68-69	0.22650140376579486
1102	70-71	0.18640781934477246
1102	72-73	0.2720335555548914
1102	74-75	0.24514156686381483
1102	76-77	0.30941517619199743
1102	78-79	0.2817078713334311
1102	80-81	0.2538268233125791
1102	82-83	0.3011667580708206
1102	84-85	0.3123007453068709
1102	86-87	0.2905173829524301
1102	88-89	0.3525210068487965
1102	90-91	0.3039993265793157
1102	92-93	0.3179875347738985
1102	94-95	0.2997775297270948
1102	96-97	0.32743901151691546
1102	98-99	0.3316313270973623
1102	100	0.3443058687343665
1103	1	0.17811495289740265
1103	2	0.17980285225462467
1103	3	0.15536748947954493
1103	4	0.14643660622351007
1103	5	0.17378293166422054
1103	6	0.18673945227752853
1103	7	0.14943041393630807
1103	8	0.16226712690806977
1103	9	0.22181495064078405
1103	10-11	0.23095717663728976
1103	12-13	0.26264390905861745
1103	14-15	0.34930145817153146
1103	16-17	0.38147822279162824
1103	18-19	0.2987609821981252
1103	20-21	0.3166629686795588
1103	22-23	0.37089319377199104
1103	24-25	0.3397633360213206
1103	26-27	0.3940346390989973
1103	28-29	0.3876687131135341
1103	30-31	0.4547105465084016
1103	32-33	0.4260791315483061
1103	34-35	0.3693930907755103
1103	36-37	0.3456725979140032
1103	38-39	0.3452350298666147
1103	40-41	0.3396507112956826
1103	42-43	0.29666637721624056
1103	44-45	0.29679718323510684
1103	46-47	0.268989833894139
1103	48-49	0.2440394636832579
1103	50-51	0.2256582186125442
1103	52-53	0.2101118958299466
1103	54-55	0.26138681479357473
1103	56-57	0.2129484285760057
1103	58-59	0.26914195156315657
1103	60-61	0.2891586788870555
1103	62-63	0.2698407507648781
1103	64-65	0.2497472638713205
1103	66-67	0.25955913227738137
1103	68-69	0.23822168203899707
1103	70-71	0.2458201043580246
1103	72-73	0.24009071711255103
1103	74-75	0.20011078116770165
1103	76-77	0.16600890199197238
1103	78-79	0.14618838251077904
1103	80-81	0.15528479913142235
1103	82-83	0.22774555489126413
1103	84-85	0.2451712276245459
1103	86-87	0.2138944221215695
1103	88-89	0.24737013178112477
1103	90-91	0.27413524471555206
1103	92-93	0.2519661548495584
1103	94-95	0.21154447880856964
1103	96-97	0.26852946148668977
1103	98-99	0.32855677279936657
1103	100	0.378395613162553
1104	1	0.07060920729612974
1104	2	0.16398106241111776
1104	3	0.15151468796105405
1104	4	0.01795105860076518
1104	5	0.07546543366399305
1104	6	0.08286107284737909
1104	7	0.04761926806986594
1104	8	0.10223489393923302
1104	9	0.08313295368476048
1104	10-11	0.17048795910537962
1104	12-13	0.16298931372620729
1104	14-15	0.2017484945795971
1104	16-17	0.2247715126356553
1104	18-19	0.2328601871228031
1104	20-21	0.20421041724858924
1104	22-23	0.2255506537619283
1104	24-25	0.30397958903556344
1104	26-27	0.24592203011429348
1104	28-29	0.20137605654567636
1104	30-31	0.2186678660255481
1104	32-33	0.2000609952317447
1104	34-35	0.28512544364508585
1104	36-37	0.2542049057993694
1104	38-39	0.20775341184770468
1104	40-41	0.16675011439773613
1104	42-43	0.18319032359315202
1104	44-45	0.13936282263660615
1104	46-47	0.15115940653386417
1104	48-49	0.16593841618891503
1104	50-51	0.15139025775840054
1104	52-53	0.1403469846103249
1104	54-55	0.10784498547915433
1104	56-57	0.08839047043395709
1104	58-59	0.02191424917621987
1104	60-61	0.055420624817543285
1104	62-63	-0.004941536977021599
1104	64-65	0.030396870825221356
1104	66-67	0.11073901631274907
1104	68-69	0.035846270640917055
1104	70-71	0.0508439439317101
1104	72-73	0.036170687859105044
1104	74-75	0.1009773872154085
1104	76-77	0.060781510585805165
1104	78-79	0.06991297560426091
1104	80-81	0.05378784842308448
1104	82-83	0.08616589941133412
1104	84-85	0.0638628351551418
1104	86-87	0.04887347634914363
1104	88-89	0.1111071075325043
1104	90-91	0.09893957883439342
1104	92-93	0.11703521349669543
1104	94-95	0.15668607277876845
1104	96-97	0.18140032834817177
1104	98-99	0.17214377147143978
1104	100	0.12912283502076605
1105	1	-0.11176810217368427
1105	2	-0.012830655678570224
1105	3	0.03377077794429795
1105	4	-0.1364307632138022
1105	5	-0.12698724404734207
1105	6	-0.09858777980726074
1105	7	-0.4391945510202646
1105	8	-0.3361247739928359
1105	9	-0.3232868728934406
1105	10-11	-0.34499456036491694
1105	12-13	-0.24843987122980593
1105	14-15	-0.2005265765008204
1105	16-17	-0.1597425520518101
1105	18-19	-0.2074622168885938
1105	20-21	-0.22071503499862644
1105	22-23	-0.19023295761066095
1105	24-25	-0.15859857195738414
1105	26-27	-0.21014371609445703
1105	28-29	-0.1927616715862328
1105	30-31	-0.20991107837965473
1105	32-33	-0.2292784461081112
1105	34-35	-0.20544974985637054
1105	36-37	-0.27888765356934186
1105	38-39	-0.3073205797579135
1105	40-41	-0.29498618738831794
1105	42-43	-0.2959417984379016
1105	44-45	-0.31032225044182127
1105	46-47	-0.313466114167511
1105	48-49	-0.3738995733321744
1105	50-51	-0.33652238113248956
1105	52-53	-0.2728646177183478
1105	54-55	-0.3129445512639748
1105	56-57	-0.26859679791530056
1105	58-59	-0.2611423494413714
1105	60-61	-0.25506347040308697
1105	62-63	-0.3228356067316085
1105	64-65	-0.3011758447315884
1105	66-67	-0.2766550839955997
1105	68-69	-0.2702336195577857
1105	70-71	-0.29252870420083354
1105	72-73	-0.2391937430272506
1105	74-75	-0.233538273820443
1105	76-77	-0.1982200061168058
1105	78-79	-0.20683495721041112
1105	80-81	-0.3014722739704965
1105	82-83	-0.2602244479334743
1105	84-85	-0.2647671065729895
1105	86-87	-0.2719033754321387
1105	88-89	-0.21200350258840928
1105	90-91	-0.19121836696391625
1105	92-93	-0.18884221509847166
1105	94-95	-0.26128007296337685
1105	96-97	-0.22482416011657946
1105	98-99	-0.18550649640611283
1105	100	-0.20660725233553734
1106	1	0.10448592333920459
1106	2	0.07627507668409095
1106	3	0.0735613826173207
1106	4	0.07504058547336001
1106	5	0.07289389882272701
1106	6	0.07629633146207482
1106	7	0.0369281702789479
1106	8	0.06092956427221452
1106	9	0.020540875945243897
1106	10-11	0.07706739073362456
1106	12-13	0.10933577998044797
1106	14-15	0.12921508795133008
1106	16-17	0.1704582854002581
1106	18-19	0.16215223852928062
1106	20-21	0.15284133348997386
1106	22-23	0.216886536008694
1106	24-25	0.16074442637628295
1106	26-27	0.1397521476128034
1106	28-29	0.16132732869727562
1106	30-31	0.12842169896779154
1106	32-33	0.14730047979175964
1106	34-35	0.2000931602734255
1106	36-37	0.2076881343084409
1106	38-39	0.1710736333578069
1106	40-41	0.2179029859104631
1106	42-43	0.23488522990768956
1106	44-45	0.26741130200111485
1106	46-47	0.26964141215835014
1106	48-49	0.289957217355699
1106	50-51	0.3152439577852064
1106	52-53	0.24881487431785843
1106	54-55	0.23189097922330149
1106	56-57	0.2638968149779757
1106	58-59	0.2805234152627065
1106	60-61	0.25124492326077075
1106	62-63	0.2686297001524096
1106	64-65	0.30958829797450704
1106	66-67	0.28780108102640156
1106	68-69	0.2838497064386871
1106	70-71	0.2653134229985099
1106	72-73	0.23015931151617508
1106	74-75	0.1963298202087458
1106	76-77	0.18244969706750425
1106	78-79	0.15160131428625334
1106	80-81	0.1622094013536639
1106	82-83	0.20410878093374407
1106	84-85	0.19944748728941164
1106	86-87	0.1723099422205081
1106	88-89	0.2051641033309437
1106	90-91	0.23559306335221208
1106	92-93	0.2275558888415432
1106	94-95	0.25785697500191773
1106	96-97	0.25564102641601494
1106	98-99	0.23710830391148363
1106	100	0.2697235800036175
1107	1	-0.06225316895820754
1107	2	-0.010755039492643448
1107	3	0.02067447098600894
1107	4	-0.07452027910905201
1107	5	-0.09368466325129532
1107	6	-0.08536605628108163
1107	7	-0.11303748113546419
1107	8	-0.04456498886735716
1107	9	-0.06917813969042186
1107	10-11	-0.02490154561154867
1107	12-13	0.009842164299499245
1107	14-15	-0.02455040420806398
1107	16-17	-0.026794447138577482
1107	18-19	0.006621023216020205
1107	20-21	-0.04996513996596974
1107	22-23	-0.02637623167299097
1107	24-25	-0.0061999740937963566
1107	26-27	-0.033187961971115953
1107	28-29	0.009309870762230332
1107	30-31	0.016156767261449545
1107	32-33	-0.015157509000637504
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2216	50-51	-0.6848522640023944
2216	52-53	-0.643970662028174
2216	54-55	-0.7236004667527993
2216	56-57	-0.7491455608492359
2216	58-59	-0.8169052615860863
2216	60-61	-0.7293760845082886
2216	62-63	-0.7669838851375417
2216	64-65	-0.6935474195323792
2216	66-67	-0.6435005072704456
2216	68-69	-0.6803503969833642
2216	70-71	-0.6663527263281992
2216	72-73	-0.6909547734862826
2216	74-75	-0.6422308458541011
2216	76-77	-0.6656957683644862
2216	78-79	-0.5880399262410023
2216	80-81	-0.5894074554081641
2216	82-83	-0.6991667217157129
2216	84-85	-0.7002109994343613
2216	86-87	-0.6858789177562947
2216	88-89	-0.6187190710854296
2216	90-91	-0.6240517842001694
2216	92-93	-0.7665387530208285
2216	94-95	-0.7108285864589305
2216	96-97	-0.8326289933485533
2216	98-99	-0.7577652245549054
2216	100	-0.738384612835457
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59.0
3	741.0
4	444.0
5	667.0
6	866.0
7	1308.0
8	2054.0
9	2731.0
10	3646.0
11	5081.0
12	7994.0
13	11532.0
14	12247.0
15	15398.0
16	20860.0
17	33333.0
18	40010.0
19	19789.0
20	11052.0
21	9683.0
22	9693.0
23	9468.0
24	10362.0
25	12463.0
26	15004.0
27	19003.0
28	22594.0
29	28261.0
30	35356.0
31	44715.0
32	56065.0
33	71785.0
34	102451.0
35	201670.0
36	323217.0
37	663875.0
38	958418.0
39	497897.0
40	17780.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34385322346674	21.031748196724493	15.121569169459706	22.502829410349065
2	13.905963504335414	22.60267246944299	40.28606757850265	23.205296447718947
3	15.234602421352417	30.394043328794346	31.790774590922855	22.580579658930386
4	10.29760836780316	18.809857481609356	39.841897448828675	31.050636701758812
5	11.736006973025592	38.26011373596333	34.52493232455603	15.478946966455043
6	26.676975074342973	40.36456849555033	18.53010026754985	14.42835616255684
7	23.323388609189312	35.08215610994396	24.171983517862316	17.422471763004413
8	25.108044316050687	33.442731360309764	21.579435150983215	19.869789172656333
9	26.620695047721345	14.883324261449667	22.348747049617344	36.14723364121165
10-11	23.67076699644681	26.966148942953815	28.290623753626225	21.07246030697315
12-13	25.381534332331583	25.95603308550321	29.263962113874165	19.39847046829104
14-15	22.580883217580947	25.22927216014683	29.230078931449295	22.95976569082293
16-17	19.78903627500779	28.836694577357306	29.180330054928337	22.193939092706568
18-19	19.382256244143182	25.669011011125082	34.737611423542205	20.211121321189538
20-21	21.606362885853077	24.689899174802065	34.13663953991609	19.567098399428776
22-23	20.632100642220145	25.254430918942607	34.64670163789549	19.46676680094176
24-25	20.313319946726335	24.71041271633713	34.59158953153392	20.384677805402617
26-27	20.256149615281572	25.432709734279403	34.4360726109816	19.87506803945743
28-29	19.862326284523558	25.125119694696885	35.020133864094106	19.992420156685455
30-31	20.79747889876392	25.277037305988266	34.052732973572894	19.872750821674913
32-33	19.795495134390535	25.454331919201255	34.848705042458064	19.90146790395015
34-35	20.460265154069564	24.71012554545861	34.43388613544422	20.395723165027608
36-37	20.51269306306525	25.485006546565476	33.90111655311204	20.10118383725723
38-39	20.223222782713904	25.24799332259226	34.1024384418897	20.426345452804135
40-41	20.66306405148729	25.15403301454331	33.63581258116871	20.54709035280069
42-43	20.309699197452748	25.626928952421334	33.93215118456071	20.131220665565206
44-45	20.730549918011683	25.014778505771456	33.30303127193951	20.95164030427735
46-47	20.928144833243344	25.745715932302325	32.57681135129999	20.749327883154336
48-49	20.780099896938946	25.68506960371729	32.10090436416058	21.43392613518319
50-51	21.44624916920464	25.903570457659512	31.10855453275584	21.541625840380004
52-53	20.970968650166945	26.31025366630995	31.530553517411807	21.18822416611129
54-55	21.239762647068897	25.94422466759952	31.35643278389627	21.459579901435312
56-57	21.30507065291265	26.113020717550338	31.21736008792726	21.364548541609746
58-59	21.050890514293748	25.45212171099488	31.85031977049019	21.646668004221176
60-61	21.547091596179975	25.957271865725083	31.029146622321345	21.466489915773593
62-63	20.821412595330546	25.982294067230537	31.863965993165174	21.33232734427374
64-65	21.136210999487208	25.47581928807736	31.677078118010453	21.710891594424975
66-67	21.307420708884546	25.892242881605064	31.41467775908836	21.385658650422027
68-69	21.31673792895269	25.86240600227668	31.472735053407312	21.348121015363315
70-71	21.671645436996016	25.854448971885063	30.938947137445734	21.534958453673188
72-73	21.182660523238035	25.997850022230175	31.382113845778036	21.43737560875375
74-75	21.493077828457075	25.533879081614174	31.061917895903523	21.911125194025228
76-77	21.58728739589521	25.876965413570296	30.84476415037156	21.69098304016293
78-79	21.27429013568259	25.858688137948967	31.268913627024865	21.598108099343573
80-81	21.33385178794863	26.01473322342944	30.970046062348818	21.681368926273116
82-83	20.96309660697541	26.27007328913719	31.435893674682546	21.330936429204854
84-85	21.192873785889894	25.50601054208834	30.97391572633243	22.327199945689333
86-87	21.357275266311497	26.131841864681316	30.776451997557864	21.734430871449327
88-89	20.7300550065108	25.854242065925682	31.152833360382736	22.262869567180786
90-91	21.15341310494338	25.826131996243067	30.5743622600131	22.44609263880045
92-93	20.69175163715241	26.18754354854206	31.173830777525787	21.946874036779747
94-95	20.79388459282899	25.420263764439532	31.094972473393977	22.690879169337496
96-97	20.885294473315813	26.20470489325507	30.657256960976824	22.252743672452286
98-99	20.263087788569624	25.96346969410885	31.20598016612785	22.56746235119368
100	20.90740716606652	25.7910747402535	30.57984819972438	22.7216698939556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5378.0
1	3711.0
2	7009.0
3	9889.5
4	4819.0
5	2964.5
6	7531.0
7	21201.0
8	35907.0
9	34096.5
10	17802.0
11	6025.0
12	4104.0
13	4028.5
14	4268.5
15	4681.5
16	5063.5
17	5572.5
18	6169.5
19	6722.0
20	7648.5
21	8824.5
22	10292.5
23	12591.0
24	15714.0
25	19423.5
26	24206.5
27	30165.5
28	37684.0
29	45629.0
30	51840.0
31	57576.0
32	62873.0
33	67717.0
34	72694.0
35	77782.0
36	83067.5
37	88328.5
38	93990.5
39	99530.5
40	104704.0
41	106887.0
42	105702.0
43	105970.0
44	105787.5
45	103402.0
46	101717.5
47	100574.5
48	99314.5
49	101628.5
50	107185.0
51	105354.5
52	98200.5
53	93303.0
54	90105.0
55	87331.0
56	78196.0
57	67094.5
58	62564.0
59	60554.5
60	53771.0
61	43310.0
62	35703.5
63	28742.5
64	21805.0
65	17168.5
66	13599.0
67	10342.0
68	7540.0
69	5334.5
70	3927.5
71	3203.5
72	2357.0
73	1516.0
74	1178.5
75	950.0
76	620.0
77	404.5
78	296.0
79	173.5
80	82.0
81	33.5
82	15.0
83	10.5
84	6.5
85	6.0
86	6.5
87	5.0
88	3.0
89	3.5
90	5.0
91	2.5
92	2.0
93	2.5
94	4.0
95	8.0
96	11.5
97	10.0
98	4.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009031474385162682
2	6.061392204807169E-5
3	3.333765712643943E-4
4	0.0020608733496344377
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	7.576740256008961E-5
24-25	1.6668828563219716E-4
26-27	4.849113763845735E-4
28-29	9.546692722571291E-4
30-31	0.0018638781029782043
32-33	0.004227821062853
34-35	0.005137029893574075
36-37	0.004000518855172731
38-39	0.0026670125701151546
40-41	0.003545914439812194
42-43	4.6975789587255556E-4
44-45	5.000648568965914E-4
46-47	0.0032883052711078892
48-49	0.0021669477132185626
50-51	6.970601035528244E-4
52-53	3.0306961024035844E-5
54-55	3.9399049331246595E-4
56-57	8.031344671369499E-4
58-59	5.455252984326452E-4
60-61	3.9399049331246595E-4
62-63	0.0
64-65	0.0
66-67	6.667531425287886E-4
68-69	7.273670645768603E-4
70-71	0.0018941850640022404
72-73	9.092088307210753E-5
74-75	0.001985105947074348
76-77	3.6368353228843014E-4
78-79	0.0010001297137931828
80-81	6.819066230408065E-4
82-83	0.001151664518913362
84-85	0.0010910505968652904
86-87	1.6668828563219716E-4
88-89	0.0016214224147859176
90-91	0.0020911803106584735
92-93	0.0028185473752353335
94-95	0.0011213575578893262
96-97	0.002151794232706545
98-99	0.00196995246656233
100	0.0031822309075237635
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3299572.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.25851553610359
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.47579555350885	33.063641990673524
2	15.12813518604855	13.996101530722044
3	5.41793066822297	7.518762899685731
4	2.649230977715497	4.901979693655169
5	1.4693563048047174	3.3985120726940385
6	0.9096812208401562	2.524830173228163
7	0.5943436979947982	1.9245420031234342
8	0.4255895802360758	1.574971376748345
9	0.3078741792420617	1.2817622253270646
>10	1.4440446973360899	11.739088183794415
>50	0.09817069864176708	3.1485988289581974
>100	0.07055305213198669	6.352425518577341
>500	0.006152487521766073	1.8491751793329985
>1k	0.002683531790566066	2.040163259553944
>5k	1.9635598467556583E-4	0.6749678634853906
>10k+	2.6180797956742107E-4	4.010477200440109
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52917	1.6037534565089047	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34207	1.036710215749194	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31950	0.9683074047179453	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13385	0.4056586733067198	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9222	0.27949079456365855	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7200	0.21821011937305806	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5871	0.17793216817211446	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4612	0.13977570424285332	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4602	0.13947263463261297	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3580	0.10849892046604832	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.0306961024035844E-5	0.0	0.0	0.0
5	0.0	3.0306961024035844E-5	0.0	0.0	0.0
6	0.0	3.0306961024035844E-5	0.0	0.0	0.0
7	0.0	3.0306961024035844E-5	0.0	0.0	0.0
8	0.0	3.0306961024035844E-5	0.0	0.0	0.0
9	0.0	3.0306961024035844E-5	0.0	0.0	0.0
10-11	0.0	3.0306961024035844E-5	0.0	0.0	0.0
12-13	0.0	3.0306961024035844E-5	0.0	0.0	0.0
14-15	0.0	3.0306961024035844E-5	0.0	0.0	0.0
16-17	0.0	3.0306961024035844E-5	0.0	1.5153480512017922E-5	0.0
18-19	0.0	3.0306961024035844E-5	0.0	1.0607436358412545E-4	0.0
20-21	0.0	3.0306961024035844E-5	0.0	3.9399049331246595E-4	0.0
22-23	0.0	3.0306961024035844E-5	0.0	0.0010758971163532724	0.0
24-25	0.0	3.0306961024035844E-5	0.0	0.002166947713218563	0.0
26-27	0.0	6.061392204807169E-5	0.0	0.0034246865957160504	0.0
28-29	0.0	6.061392204807169E-5	0.0	0.006985754516040263	0.0
30-31	0.0	6.061392204807169E-5	0.0	0.015577777966354425	0.0
32-33	0.0	6.061392204807169E-5	0.0	0.027897557622624997	0.0
34-35	0.0	6.061392204807169E-5	0.0	0.04479368839352498	0.0
36-37	0.0	6.061392204807169E-5	0.0	0.06761483004462397	0.0
38-39	0.0	6.061392204807169E-5	0.0	0.1008767197685033	0.0
40-41	0.0	6.061392204807169E-5	0.0	0.13804820746448326	0.0
42-43	0.0	6.061392204807169E-5	0.0	0.17293151960314854	0.0
44-45	0.0	6.061392204807169E-5	0.0	0.2138156100245729	0.0
46-47	0.0	6.061392204807169E-5	0.0	0.2594578933267708	0.0
48-49	0.0	6.061392204807169E-5	0.0	0.3104493552497112	0.0
50-51	0.0	6.061392204807169E-5	0.0	0.36971461753221324	0.0
52-53	0.0	6.061392204807169E-5	0.0	0.4294647911910999	0.0
54-55	0.0	6.061392204807169E-5	0.0	0.49051816417402017	0.0
56-57	0.0	6.061392204807169E-5	0.0	0.5599059514385503	0.0
58-59	0.0	6.061392204807169E-5	0.0	0.6368856324396013	0.0
60-61	0.0	6.061392204807169E-5	0.0	0.7258668700061706	0.0
62-63	0.0	6.061392204807169E-5	0.0	0.8174241992597828	0.0
64-65	0.0	6.061392204807169E-5	0.0	0.9113000110317337	0.0
66-67	0.0	6.061392204807169E-5	0.0	1.0135860044878546	0.0
68-69	0.0	6.061392204807169E-5	0.0	1.1363140431546879	0.0
70-71	0.0	6.061392204807169E-5	0.0	1.2745743993463394	0.0
72-73	0.0	6.061392204807169E-5	0.0	1.4260485905444706	0.0
74-75	0.0	6.061392204807169E-5	0.0	1.584402461895058	0.0
76-77	0.0	6.061392204807169E-5	0.0	1.7465143964126257	0.0
78-79	0.0	6.061392204807169E-5	0.0	1.930280654581867	0.0
80-81	0.0	6.061392204807169E-5	0.0	2.1447175573074326	0.0
82-83	0.0	6.061392204807169E-5	0.0	2.377187101842299	0.0
84-85	0.0	6.061392204807169E-5	0.0	2.6148542901927887	0.0
86-87	0.0	6.061392204807169E-5	0.0	2.8661899179651176	0.0
88	0.0	6.061392204807169E-5	0.0	3.063306392465447	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10565	0.0	63.70899	1
GTATCAA	23060	0.0	50.04024	1
ATCAACG	32280	0.0	35.540913	3
TATCAAC	32325	0.0	35.535053	2
TCAACGC	32545	0.0	35.265953	4
CAACGCA	33885	0.0	33.842583	5
AACGCAG	34705	0.0	33.11067	6
ACGCAGA	37535	0.0	30.476515	7
CGCAGAG	37830	0.0	30.11462	8
GTGGTAT	3400	0.0	29.999084	1
TGGTATC	3425	0.0	28.405605	2
GCAGAGT	39955	0.0	28.265963	9
GTACATG	17960	0.0	25.569098	1
TACATGG	17600	0.0	25.449223	2
AGAGTAC	38840	0.0	24.128319	10-11
ACATGGG	17855	0.0	23.953878	3
AGTACTT	30655	0.0	23.610249	12-13
GAGTACT	30305	0.0	22.681026	12-13
CAGAGTA	39480	0.0	22.451517	10-11
CATGGGG	10600	0.0	21.149826	4
>>END_MODULE