FastQCFastQC Report
Wed 25 May 2016
SRR2431359_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431359_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3225750
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA374011.159451290397582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43150.052.8278431
GTATCAA80900.040.9002651
TCAACGC97800.032.7748534
ATCAACG98300.032.560333
CAACGCA106850.030.0868555
TATCAAC112800.029.0836092
AACGCAG111500.028.7478076
GTACATG195950.026.9362071
TACATGG187900.026.5643792
ACGCAGA121450.026.0443047
CGCAGAG122800.025.6814388
ACATGGG198050.024.3245163
GTGGTAT19600.023.9798161
CATGGGT40300.023.6747954
GCAGAGT132100.022.8416489
CATGGGA69050.020.3517954
TGGTATC21050.019.871922
CATGGGG102200.019.8208184
CAGAGTA130750.018.9975910-11
AGAGTAC124350.018.12333110-11
Produced by FastQC (version 0.11.5)