##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431359_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3225750
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.896665891653104	33.0	31.0	34.0	27.0	34.0
2	30.99670712237464	34.0	31.0	34.0	27.0	34.0
3	31.056727892738124	34.0	31.0	34.0	27.0	34.0
4	34.20290165077889	37.0	35.0	37.0	30.0	37.0
5	34.40525738200419	37.0	35.0	37.0	31.0	37.0
6	34.37965341393475	37.0	35.0	37.0	31.0	37.0
7	34.35260544059521	37.0	35.0	37.0	31.0	37.0
8	34.29687235526622	37.0	35.0	37.0	31.0	37.0
9	35.71400170502984	39.0	37.0	39.0	30.0	39.0
10-11	35.77115446020305	39.0	37.0	39.0	30.0	39.0
12-13	35.70666976672092	39.0	37.0	39.0	30.0	39.0
14-15	37.04159745795552	41.0	38.0	41.0	30.0	41.0
16-17	37.047054638456174	41.0	38.0	41.0	30.0	41.0
18-19	37.04486538014415	41.0	38.0	41.0	30.0	41.0
20-21	37.08311260947067	41.0	38.0	41.0	30.0	41.0
22-23	37.08923614663257	40.0	38.0	41.0	30.0	41.0
24-25	37.106387506781374	40.5	38.0	41.0	30.0	41.0
26-27	37.11493466635666	40.0	38.0	41.0	30.0	41.0
28-29	37.0882890800589	40.0	38.0	41.0	30.0	41.0
30-31	37.01686755018213	40.0	38.0	41.0	30.0	41.0
32-33	36.93866124157172	40.0	38.0	41.0	30.0	41.0
34-35	36.86557932263815	40.0	38.0	41.0	30.0	41.0
36-37	36.759723010152676	40.0	37.0	41.0	30.0	41.0
38-39	36.65238192668372	40.0	37.0	41.0	29.0	41.0
40-41	36.55678942881501	40.0	37.0	41.0	29.0	41.0
42-43	36.4183286057506	40.0	37.0	41.0	28.0	41.0
44-45	36.27116407037123	40.0	36.5	41.0	28.0	41.0
46-47	36.13266480663412	40.0	36.0	41.0	28.0	41.0
48-49	35.98408447647834	40.0	35.5	41.0	28.0	41.0
50-51	35.828672556769746	40.0	35.0	41.0	27.5	41.0
52-53	35.643118499573745	39.5	35.0	41.0	27.0	41.0
54-55	35.45873703789816	39.0	35.0	41.0	27.0	41.0
56-57	35.24240176703093	39.0	35.0	41.0	27.0	41.0
58-59	34.99638409672169	39.0	34.5	41.0	26.5	41.0
60-61	34.79804867085174	38.0	34.0	40.0	26.0	41.0
62-63	34.61294908160893	38.0	34.0	40.0	26.0	41.0
64-65	34.34515523521662	37.5	34.0	40.0	26.0	41.0
66-67	34.02816631791056	37.0	34.0	40.0	26.0	41.0
68-69	33.70095698674727	36.5	33.5	39.5	25.0	41.0
70-71	33.4482520344106	36.0	33.0	39.0	25.0	41.0
72-73	33.19958660776564	35.5	33.0	39.0	25.0	41.0
74-75	32.856867240176705	35.0	33.0	38.5	24.0	40.5
76-77	32.52352863675114	35.0	33.0	37.0	24.0	39.5
78-79	32.19694086646516	35.0	33.0	37.0	23.0	39.0
80-81	31.87488181043168	35.0	33.0	36.5	22.0	39.0
82-83	31.56869131209796	35.0	32.0	36.0	20.5	38.0
84-85	31.297545067038673	35.0	32.0	36.0	20.0	37.0
86-87	31.05325676199333	35.0	32.0	35.0	19.5	37.0
88-89	30.822423777416105	35.0	32.0	35.0	18.0	36.5
90-91	30.632286910020923	35.0	32.0	35.0	17.5	36.0
92-93	30.44626908470898	35.0	32.0	35.0	11.5	36.0
94-95	30.238174378051617	35.0	31.0	35.0	4.5	36.0
96-97	30.06445880802914	35.0	31.0	35.0	2.0	35.0
98-99	29.94741997984965	35.0	31.0	35.0	2.0	35.0
100	29.903321088119043	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3465139954222529
1101	2	0.3487776220058798
1101	3	0.3537231967975103
1101	4	0.3506806678849017
1101	5	0.19498443791212594
1101	6	0.200473869120799
1101	7	0.21008598425117953
1101	8	0.25470590023660833
1101	9	0.3317500926366037
1101	10-11	0.3140460894861974
1101	12-13	0.3402174481135347
1101	14-15	0.39781251894493863
1101	16-17	0.39592112093547627
1101	18-19	0.348182498685631
1101	20-21	0.2946155741581151
1101	22-23	0.35077093217403643
1101	24-25	0.3377487621870259
1101	26-27	0.33206779896128324
1101	28-29	0.29200106770470313
1101	30-31	0.37574620069324993
1101	32-33	0.37716668260867436
1101	34-35	0.4202918609600559
1101	36-37	0.41967450948307317
1101	38-39	0.37796650506670204
1101	40-41	0.33831827239153256
1101	42-43	0.37610544450738814
1101	44-45	0.3808629208092853
1101	46-47	0.3429683604708913
1101	48-49	0.3657960336879711
1101	50-51	0.3580310710033885
1101	52-53	0.3161241574680389
1101	54-55	0.3763622244030742
1101	56-57	0.3575627379944848
1101	58-59	0.3699508163846943
1101	60-61	0.37318396243993135
1101	62-63	0.3705062411669431
1101	64-65	0.3289994688732918
1101	66-67	0.2744139037377522
1101	68-69	0.32885673319557185
1101	70-71	0.29316216133337747
1101	72-73	0.31903741347299786
1101	74-75	0.34662182355025806
1101	76-77	0.34121703341649834
1101	78-79	0.3394212170177724
1101	80-81	0.3080331244553882
1101	82-83	0.3395495306927998
1101	84-85	0.34118872969325764
1101	86-87	0.30054536500874107
1101	88-89	0.36339346496122005
1101	90-91	0.3595468203172416
1101	92-93	0.3927491213711569
1101	94-95	0.40648599005070096
1101	96-97	0.3797480431162157
1101	98-99	0.40841997988730583
1101	100	0.4370140402859697
1102	1	0.00541126112113588
1102	2	0.06402182358522523
1102	3	0.11954968917098441
1102	4	0.05636564484996143
1102	5	0.0015662195395620415
1102	6	0.03737608378479962
1102	7	0.11453827963878638
1102	8	0.09803370563529512
1102	9	0.09924615356097632
1102	10-11	0.13739385202369192
1102	12-13	0.11759565974786312
1102	14-15	0.10528670385603789
1102	16-17	0.18600337922062948
1102	18-19	0.09665114609234138
1102	20-21	0.0644467934223556
1102	22-23	-0.0020717674057948443
1102	24-25	0.08004091367383381
1102	26-27	0.08562214976109317
1102	28-29	0.180175966884633
1102	30-31	0.13272813836381658
1102	32-33	0.1331942493829672
1102	34-35	0.1380476982226142
1102	36-37	0.06166953096433758
1102	38-39	0.07308706484608507
1102	40-41	0.05291039440915313
1102	42-43	0.019995644779612576
1102	44-45	0.08546433533008013
1102	46-47	-0.004327177016605788
1102	48-49	-0.1429775395475943
1102	50-51	-0.1456499381768097
1102	52-53	-0.11780935363736234
1102	54-55	-0.13919823259725206
1102	56-57	-0.10835665830100538
1102	58-59	-0.1188235569096392
1102	60-61	-0.08638319943285921
1102	62-63	-0.08420752989211877
1102	64-65	-0.054049882298045304
1102	66-67	-0.004373699024419864
1102	68-69	-0.03243154718893493
1102	70-71	-0.0373556793438965
1102	72-73	-0.04547184229924284
1102	74-75	-0.06709199648337716
1102	76-77	-0.06139312137692343
1102	78-79	-0.10401552514291978
1102	80-81	-0.11338849131712081
1102	82-83	-0.10118453272758643
1102	84-85	-0.11656062054925087
1102	86-87	-0.06449981403554261
1102	88-89	-0.08111454322597922
1102	90-91	-0.05265353761934222
1102	92-93	-0.060193015592144405
1102	94-95	0.0634032686180781
1102	96-97	0.08344801938887869
1102	98-99	0.02763522778308669
1102	100	0.034851939513185215
1103	1	0.12747591883249143
1103	2	0.14553257303945344
1103	3	0.19429819537081627
1103	4	0.16261025785130556
1103	5	0.1084428689179191
1103	6	0.1794398700087214
1103	7	0.22112181597253056
1103	8	0.1497534685574351
1103	9	0.22094609690389433
1103	10-11	0.1845354460594706
1103	12-13	0.15400467292458586
1103	14-15	0.20712487155268633
1103	16-17	0.1895430689211821
1103	18-19	0.12694341988831326
1103	20-21	0.13062425197366423
1103	22-23	0.19277623395530696
1103	24-25	0.23805382978307676
1103	26-27	0.23646305193152983
1103	28-29	0.22779927091602303
1103	30-31	0.2604994787822008
1103	32-33	0.246189302079884
1103	34-35	0.275262301689601
1103	36-37	0.27031360468343735
1103	38-39	0.19492690896007048
1103	40-41	0.19724342781769622
1103	42-43	0.14266350594563448
1103	44-45	0.10493531958257307
1103	46-47	0.09477438496341506
1103	48-49	0.11425364817953465
1103	50-51	0.05861761511911823
1103	52-53	0.0969129809006617
1103	54-55	0.037738336221551094
1103	56-57	0.03138926255545016
1103	58-59	0.0874768084079065
1103	60-61	0.025012289049364256
1103	62-63	0.05577797200975709
1103	64-65	0.10140277950326748
1103	66-67	0.016033848223095504
1103	68-69	0.05217974237950784
1103	70-71	0.12761416074336296
1103	72-73	0.10743730856833622
1103	74-75	0.11210783974353689
1103	76-77	0.10726824273592683
1103	78-79	0.04776989572518886
1103	80-81	0.03537490650211694
1103	82-83	0.018645690497990586
1103	84-85	0.028407127136208743
1103	86-87	0.0987440805606532
1103	88-89	0.07514107256412927
1103	90-91	0.10691469235849738
1103	92-93	0.11421005191879985
1103	94-95	0.0880587710940155
1103	96-97	0.049679103535879676
1103	98-99	0.05589572951857491
1103	100	0.027714998263501656
1104	1	0.18101023587566445
1104	2	0.21301082433483742
1104	3	0.19355188049544836
1104	4	0.1584250032410992
1104	5	0.2474970981122766
1104	6	0.22650087707884126
1104	7	0.1727553643287152
1104	8	0.1603845073648742
1104	9	0.184408154329887
1104	10-11	0.2358954248388727
1104	12-13	0.277042539332399
1104	14-15	0.31716270018680603
1104	16-17	0.31666675554933477
1104	18-19	0.2607492113808547
1104	20-21	0.22047576080946385
1104	22-23	0.19713934171242897
1104	24-25	0.2998652367319252
1104	26-27	0.33850271192087433
1104	28-29	0.37659262456990916
1104	30-31	0.39213974421979003
1104	32-33	0.38670096169653334
1104	34-35	0.3033449546634799
1104	36-37	0.3190043753155507
1104	38-39	0.33850744504831454
1104	40-41	0.3395907992471976
1104	42-43	0.3198701684309526
1104	44-45	0.35424924209522857
1104	46-47	0.3731421599237592
1104	48-49	0.33840839274454027
1104	50-51	0.2857040280842753
1104	52-53	0.2603775978277554
1104	54-55	0.25150405592727054
1104	56-57	0.19215288797539642
1104	58-59	0.18494728862229692
1104	60-61	0.21866357281739113
1104	62-63	0.1662194295114645
1104	64-65	0.26951341074504853
1104	66-67	0.24012725387397182
1104	68-69	0.22902861928577067
1104	70-71	0.22422324754654
1104	72-73	0.22498895615682102
1104	74-75	0.23665816337190648
1104	76-77	0.24939778428472437
1104	78-79	0.2313009270026356
1104	80-81	0.21574819661800504
1104	82-83	0.3010675518256072
1104	84-85	0.21481182960806677
1104	86-87	0.31261344273347547
1104	88-89	0.284772900955371
1104	90-91	0.26202142429689346
1104	92-93	0.2649113477937135
1104	94-95	0.28302644043657565
1104	96-97	0.2574008114072939
1104	98-99	0.2707069336380954
1104	100	0.20013517091447497
1105	1	0.34332898445906324
1105	2	0.35428167154051593
1105	3	0.3707837894088506
1105	4	0.42648752991284056
1105	5	0.3714056296643946
1105	6	0.4201328859593758
1105	7	0.40517574435659753
1105	8	0.41253178747673047
1105	9	0.453383021545946
1105	10-11	0.47739320394118323
1105	12-13	0.4491667556408885
1105	14-15	0.5364867155753572
1105	16-17	0.5734691064230262
1105	18-19	0.5407909635773933
1105	20-21	0.5131935612920415
1105	22-23	0.46679609871733874
1105	24-25	0.5190719568257762
1105	26-27	0.5038040630000253
1105	28-29	0.5600615875696064
1105	30-31	0.454113132085908
1105	32-33	0.4951867883590211
1105	34-35	0.5287352806305492
1105	36-37	0.5215782776348874
1105	38-39	0.4758491955839972
1105	40-41	0.49174046469194366
1105	42-43	0.4783398571336832
1105	44-45	0.4904421887151784
1105	46-47	0.5105810240637254
1105	48-49	0.5324462039177433
1105	50-51	0.552351741868371
1105	52-53	0.5079674136194399
1105	54-55	0.4672187521750857
1105	56-57	0.44788748203929174
1105	58-59	0.4427618288920101
1105	60-61	0.4627969969050838
1105	62-63	0.5112719755857285
1105	64-65	0.5506840808693312
1105	66-67	0.5633427180856216
1105	68-69	0.5816457261933863
1105	70-71	0.5994428127565428
1105	72-73	0.5437235922597452
1105	74-75	0.47773427081813935
1105	76-77	0.5439145397450815
1105	78-79	0.5324961956258676
1105	80-81	0.5037736185496335
1105	82-83	0.5541637163729618
1105	84-85	0.6102965965223568
1105	86-87	0.5112924512964732
1105	88-89	0.5996406364647306
1105	90-91	0.5974558547732904
1105	92-93	0.56861703142593
1105	94-95	0.5662810241075213
1105	96-97	0.5664798269882994
1105	98-99	0.47959541336244627
1105	100	0.47540155074650414
1106	1	0.05719996566385532
1106	2	0.12396612148094377
1106	3	0.155398617086572
1106	4	0.06225881647377918
1106	5	0.128196500938202
1106	6	0.12367031808548035
1106	7	0.07593420955630137
1106	8	0.07155897807795242
1106	9	0.11006563584467699
1106	10-11	0.08638747934922719
1106	12-13	0.06082770355694578
1106	14-15	0.055071106326089136
1106	16-17	0.06664050084895479
1106	18-19	0.1466800507900956
1106	20-21	0.17468695876945617
1106	22-23	0.12711981358944513
1106	24-25	0.16565660490298484
1106	26-27	0.18741151283231972
1106	28-29	0.20392198749092927
1106	30-31	0.2713302798985282
1106	32-33	0.2420923336294578
1106	34-35	0.25216725501411474
1106	36-37	0.265171021120274
1106	38-39	0.2193745868597503
1106	40-41	0.2469661868259152
1106	42-43	0.2839774515184317
1106	44-45	0.30881951926649975
1106	46-47	0.25647144402469735
1106	48-49	0.25780787329333066
1106	50-51	0.23152014844790614
1106	52-53	0.22223040266945304
1106	54-55	0.30956046468283915
1106	56-57	0.31321513635851517
1106	58-59	0.260080247127334
1106	60-61	0.2655861300722293
1106	62-63	0.3577641844269195
1106	64-65	0.3656261028606025
1106	66-67	0.37028050295192116
1106	68-69	0.44665843226498225
1106	70-71	0.35750650475056744
1106	72-73	0.28508790222932134
1106	74-75	0.23405593630155153
1106	76-77	0.3584778352580287
1106	78-79	0.3254282487522602
1106	80-81	0.3120012870033655
1106	82-83	0.26673847305993803
1106	84-85	0.2780327898381856
1106	86-87	0.25026745258195504
1106	88-89	0.2797588913576803
1106	90-91	0.3244141243034129
1106	92-93	0.3472444759410571
1106	94-95	0.4061254937670924
1106	96-97	0.3995822735959358
1106	98-99	0.44424936112194047
1106	100	0.4871905623983679
1107	1	0.0820160710399449
1107	2	0.08708937919733017
1107	3	0.1085565045185568
1107	4	0.12645696879605595
1107	5	0.09498673418592318
1107	6	0.12017805513496427
1107	7	0.1497195956381674
1107	8	0.14516820151015253
1107	9	0.11359009032359779
1107	10-11	0.12198430800723514
1107	12-13	0.13721840762130455
1107	14-15	0.09599789089991617
1107	16-17	0.12162452212602659
1107	18-19	0.08583986432780932
1107	20-21	0.06856733678997529
1107	22-23	0.1047608705901979
1107	24-25	0.07799919734021188
1107	26-27	0.10473225376778572
1107	28-29	0.06042713098870678
1107	30-31	0.07836454316100827
1107	32-33	0.07908261630380764
1107	34-35	0.10301924296528853
1107	36-37	0.12586862438433855
1107	38-39	0.0777575487993758
1107	40-41	0.10201133886252478
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2216	36-37	-0.5530928676559626
2216	38-39	-0.5347202277916594
2216	40-41	-0.5929031048217581
2216	42-43	-0.5650242305932807
2216	44-45	-0.5910068355471552
2216	46-47	-0.4835427006369102
2216	48-49	-0.599643946453071
2216	50-51	-0.5544384362424424
2216	52-53	-0.5379788150431608
2216	54-55	-0.7579026772812227
2216	56-57	-0.6619735537517357
2216	58-59	-0.6307054466504738
2216	60-61	-0.6029665725800584
2216	62-63	-0.7219317377904133
2216	64-65	-0.7333461382409894
2216	66-67	-0.7834455285783406
2216	68-69	-0.7564188426816116
2216	70-71	-0.8084951303204022
2216	72-73	-0.7524036426045484
2216	74-75	-0.7763688577330328
2216	76-77	-0.7113365322793186
2216	78-79	-0.6583134197663014
2216	80-81	-0.6984992588313403
2216	82-83	-0.7198796551406552
2216	84-85	-0.6294264104369205
2216	86-87	-0.6102734022941441
2216	88-89	-0.7150729542449028
2216	90-91	-0.6693364332914769
2216	92-93	-0.7258423044776698
2216	94-95	-0.7949574974395333
2216	96-97	-0.8705286938697228
2216	98-99	-0.8777312321427999
2216	100	-0.8186350267868043
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68635.0
3	16264.0
4	12953.0
5	12328.0
6	7718.0
7	5916.0
8	6477.0
9	6709.0
10	6491.0
11	7179.0
12	8210.0
13	9042.0
14	9278.0
15	9449.0
16	9391.0
17	9399.0
18	9339.0
19	9114.0
20	8576.0
21	8993.0
22	11245.0
23	18067.0
24	31579.0
25	39239.0
26	38074.0
27	28228.0
28	28130.0
29	32688.0
30	38840.0
31	46170.0
32	60159.0
33	81002.0
34	117874.0
35	195938.0
36	344388.0
37	639272.0
38	865153.0
39	366346.0
40	1897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.37625254088748	21.974875245796085	13.571477950971294	22.07739426234514
2	15.449594946660433	22.839260847406557	39.246742150967776	22.464402054965234
3	17.43634813609238	30.20110051925909	29.184499728745255	23.17805161590328
4	12.021653285782575	19.967753195694886	38.38774038625484	29.6228531322677
5	12.30008906458624	41.24313454022616	33.83911767468579	12.617658720501812
6	27.47791986359761	41.058668526699215	18.388777803611564	13.074633806091606
7	25.186049755870727	35.862853599938	22.650887390529334	16.30020925366194
8	24.181105169340462	37.72524219173835	20.370208478648376	17.723444160272805
9	25.399953499186235	19.52411067193676	20.782980702162288	34.292955126714716
10-11	23.0132217313803	29.925273192280866	27.83269007207626	19.228815004262575
12-13	23.54762458343021	28.4542199488491	27.332015810276676	20.666139657444006
14-15	21.65347593582888	29.162830349531117	25.378656126482213	23.80503758815779
16-17	21.017840812214214	31.99964349376114	25.859862047585835	21.122653646438813
18-19	21.01089669069209	30.9977059598543	27.677020847864835	20.314376501588775
20-21	23.01054018445323	29.76083081453925	26.863954119197086	20.364674881810434
22-23	22.42982252189413	29.948988607300624	26.93508486398512	20.68610400682012
24-25	21.7100519259087	30.182345191040845	26.97368053940944	21.133922343641014
26-27	21.456420987367277	30.251476400837014	27.062931101294275	21.229171510501434
28-29	21.573835542121987	29.951840657211505	27.48979307137875	20.984530729287762
30-31	21.895853677439355	30.151701154770212	26.96476788343796	20.987677284352475
32-33	21.319863597612958	30.09931023792916	27.361357823761917	21.21946834069596
34-35	21.953328683251957	29.59758195768426	27.28760753313183	21.161481825931954
36-37	21.984174223048903	29.879438890180577	26.880864915136016	21.255521971634504
38-39	21.67013872742773	29.942493993644888	27.105417344803534	21.281949934123848
40-41	21.63817716810044	29.748260094551654	27.244888785553744	21.368673951794158
42-43	21.53897543207006	29.87767185925754	27.362892350616136	21.22046035805627
44-45	21.480477408354645	29.698876230334033	27.2447337828412	21.575912578470124
46-47	21.703681314423	29.701635278617378	27.20283654963962	21.391846857320004
48-49	21.540850964891884	29.636766643416262	27.28253894443153	21.539843447260328
50-51	21.65423544912036	29.6231884057971	27.027807486631016	21.694768658451522
52-53	21.553933193830893	29.752693172130513	27.138742928001243	21.554630706037354
54-55	21.634395101914283	29.86674416802294	27.041354723707666	21.45750600635511
56-57	21.671859257537008	29.772719522591647	26.938820429357513	21.616600790513836
58-59	21.825358443772767	29.24643881267922	27.169309462915603	21.75889328063241
60-61	21.92239014182748	29.578454622955903	26.9955824226924	21.50357281252422
62-63	21.465953654188947	29.67010772688522	27.237402154537705	21.626536464388128
64-65	21.80476196307246	29.487891108717523	27.037886853892406	21.669460074317612
66-67	21.63203906068356	29.45316593040378	27.017158800279006	21.89763620863365
68-69	21.864465628148494	29.689250561884833	27.009951174145545	21.436332635821127
70-71	22.00725412694722	29.39454390451833	27.091017592807876	21.507184375726577
72-73	21.75980779663644	29.485623498411222	27.1431760055801	21.61139269937224
74-75	21.68221343873518	29.402108036890645	27.21962334340851	21.69605518096567
76-77	21.840533209331163	29.42537394404402	26.93401534526854	21.800077501356274
78-79	21.841866232659072	29.44372626520964	27.097775711074945	21.616631791056342
80-81	21.744633031078045	29.546880570409982	27.081779431140045	21.626706967371927
82-83	21.6256286524463	29.593034051936467	27.272642725766943	21.508694569850288
84-85	21.726155969463072	29.194843716189865	27.084455816877647	21.994544497469413
86-87	21.996840386507035	29.473967134687516	26.93868111557421	21.590511363231236
88-89	21.627849119600825	29.493352926177295	27.101227885391165	21.777570068830716
90-91	21.684147740677233	29.354656802490265	26.985884212285228	21.97531124454727
92-93	21.646003948913986	29.50072495959788	27.11488400801439	21.738387083473743
94-95	21.69682812541173	29.139627274122322	27.27354880111108	21.889995799354868
96-97	21.79585826332269	29.544052453730973	26.88785379917537	21.772235483770963
98-99	21.353516530512188	29.52909403936185	27.106167942854952	22.011221487271005
100	21.503519266683035	29.426023476658507	27.025531424149758	22.0449258325087
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32104.0
1	21904.0
2	9343.0
3	5521.0
4	3357.0
5	2358.5
6	2037.0
7	2227.5
8	2654.5
9	2740.0
10	2076.5
11	1435.5
12	1322.0
13	1324.0
14	1350.5
15	1398.0
16	1562.0
17	1927.5
18	2394.0
19	2920.0
20	3773.5
21	5007.0
22	6786.0
23	9222.0
24	12572.0
25	16478.0
26	21361.0
27	27922.0
28	35464.5
29	43397.5
30	50670.0
31	57751.0
32	65097.0
33	71604.5
34	76757.5
35	82976.0
36	89583.5
37	94801.0
38	101665.5
39	107012.5
40	111077.0
41	114518.5
42	116842.5
43	120960.5
44	121042.5
45	117756.0
46	115748.5
47	112968.5
48	108188.5
49	105900.0
50	106181.5
51	101228.5
52	92150.5
53	84824.5
54	80367.5
55	77490.0
56	69930.0
57	60110.5
58	54414.0
59	50277.5
60	44341.5
61	36921.0
62	31062.5
63	26121.5
64	21478.0
65	17479.0
66	14021.0
67	10957.0
68	8440.5
69	6534.5
70	4787.0
71	3573.0
72	2796.0
73	2128.0
74	1712.5
75	1356.5
76	966.5
77	709.0
78	561.5
79	424.0
80	294.5
81	212.5
82	167.5
83	134.5
84	110.0
85	90.5
86	81.5
87	74.0
88	54.5
89	48.0
90	44.0
91	35.0
92	28.0
93	18.0
94	22.5
95	22.0
96	18.5
97	17.5
98	21.5
99	22.0
100	44.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.170037975664574E-4
2	6.200108501898783E-5
3	0.0
4	1.2400217003797565E-4
5	3.100054250949391E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	1.5500271254746957E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	3.100054250949391E-5
84-85	4.960086801519026E-4
86-87	0.0012090211578702627
88-89	0.0025575447570332483
90-91	0.002201038518174068
92-93	0.0016430287530031778
94-95	0.0017050298380221654
96-97	0.0015500271254746956
98-99	0.0019685344493528636
100	0.0024180423157405253
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3225750.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.284300767297175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.05535980181443	38.71932705308475
2	14.444121309100458	15.104015656886688
3	5.0619606590534385	7.9398322071052725
4	2.282108666802863	4.772738236751057
5	1.2057619875591004	3.152121120565701
6	0.7258300107579888	2.2769708753040736
7	0.43955547076326706	1.6087295306108287
8	0.31145943078791094	1.3027550844901048
9	0.2251923479975432	1.059662200787766
>10	1.0746926948286502	10.406994165913057
>50	0.10094693703637855	3.7050168130745966
>100	0.06784093140069591	6.561817164171019
>500	0.004040725777701314	1.4287519912599875
>1k	0.0010696038817936473	0.7992709616372138
>5k	0.0	0.0
>10k+	5.942243787742486E-5	1.161996938357826
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37401	1.159451290397582	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.100054250949391E-5	0.0
8	0.0	0.0	0.0	3.100054250949391E-5	0.0
9	0.0	0.0	0.0	3.100054250949391E-5	0.0
10-11	0.0	0.0	0.0	3.100054250949391E-5	0.0
12-13	0.0	0.0	0.0	4.6500813764240866E-5	0.0
14-15	0.0	0.0	0.0	6.200108501898783E-5	0.0
16-17	0.0	0.0	0.0	6.200108501898783E-5	0.0
18-19	0.0	0.0	0.0	9.300162752848175E-5	0.0
20-21	0.0	0.0	0.0	1.395024412927226E-4	0.0
22-23	0.0	0.0	0.0	3.1000542509493915E-4	0.0
24-25	0.0	0.0	0.0	8.215143765015888E-4	0.0
26-27	0.0	0.0	0.0	0.0017205301092769122	0.0
28-29	0.0	0.0	0.0	0.0038750678136867398	0.0
30-31	0.0	0.0	0.0	0.00978067116174533	0.0
32-33	0.0	0.0	0.0	0.02094086646516314	0.0
34-35	0.0	0.0	0.0	0.03689064558629776	0.0
36-37	0.0	0.0	0.0	0.06028055490971092	0.0
38-39	0.0	0.0	0.0	0.0934511353948694	0.0
40-41	0.0	0.0	0.0	0.12663721615128265	0.0
42-43	0.0	0.0	0.0	0.15852127412229713	0.0
44-45	0.0	0.0	0.0	0.19976749593117882	0.0
46-47	0.0	0.0	0.0	0.2487793536386887	0.0
48-49	0.0	0.0	0.0	0.3010152677671859	0.0
50-51	0.0	0.0	0.0	0.35667674184298226	0.0
52-53	0.0	0.0	0.0	0.40995117414554755	0.0
54-55	0.0	0.0	0.0	0.46804619080833915	1.5500271254746957E-5
56-57	0.0	0.0	0.0	0.5384019220336356	3.100054250949391E-5
58-59	0.0	0.0	0.0	0.6171898008215144	3.100054250949391E-5
60-61	0.0	0.0	0.0	0.7039603193055879	3.100054250949391E-5
62-63	0.0	0.0	0.0	0.7912113461985585	3.100054250949391E-5
64-65	0.0	0.0	0.0	0.8842129737270402	3.100054250949391E-5
66-67	0.0	0.0	0.0	0.9879717895063164	3.100054250949391E-5
68-69	0.0	0.0	0.0	1.1101449275362318	3.100054250949391E-5
70-71	0.0	0.0	0.0	1.2496473688289544	3.100054250949391E-5
72-73	0.0	0.0	0.0	1.3949469115709525	3.100054250949391E-5
74-75	0.0	0.0	0.0	1.5428815004262575	3.100054250949391E-5
76-77	0.0	0.0	0.0	1.7025342943501511	3.100054250949391E-5
78-79	0.0	0.0	0.0	1.8894985662249089	3.100054250949391E-5
80-81	0.0	0.0	0.0	2.101883282957452	3.100054250949391E-5
82-83	0.0	0.0	0.0	2.3281562427342477	3.100054250949391E-5
84-85	0.0	0.0	0.0	2.5578857630008525	3.100054250949391E-5
86-87	0.0	0.0	0.0	2.79569092459118	3.100054250949391E-5
88	0.0	0.0	0.0	2.9909013407734637	3.100054250949391E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4315	0.0	52.827843	1
GTATCAA	8090	0.0	40.900265	1
TCAACGC	9780	0.0	32.774853	4
ATCAACG	9830	0.0	32.56033	3
CAACGCA	10685	0.0	30.086855	5
TATCAAC	11280	0.0	29.083609	2
AACGCAG	11150	0.0	28.747807	6
GTACATG	19595	0.0	26.936207	1
TACATGG	18790	0.0	26.564379	2
ACGCAGA	12145	0.0	26.044304	7
CGCAGAG	12280	0.0	25.681438	8
ACATGGG	19805	0.0	24.324516	3
GTGGTAT	1960	0.0	23.979816	1
CATGGGT	4030	0.0	23.674795	4
GCAGAGT	13210	0.0	22.841648	9
CATGGGA	6905	0.0	20.351795	4
TGGTATC	2105	0.0	19.87192	2
CATGGGG	10220	0.0	19.820818	4
CAGAGTA	13075	0.0	18.99759	10-11
AGAGTAC	12435	0.0	18.123331	10-11
>>END_MODULE