FastQCFastQC Report
Wed 25 May 2016
SRR2431359_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431359_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3225750
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307660.9537626908470898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216210.670262729597768No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178670.5538866930171278No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72540.22487793536386888No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52330.16222583895218165No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49160.1523986669766721No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38340.11885607998139967No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38290.11870107726885219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84550.063.208461
GTATCAA179800.047.5261421
GTGGTAT24650.035.085631
ATCAACG244050.034.760533
TCAACGC245600.034.5023424
TATCAAC250800.033.9749032
CAACGCA253850.033.344015
AACGCAG261400.032.434876
TATAACG1900.032.1571662
TGGTATC27750.030.6551572
ACGCAGA295100.028.5715947
CGCAGAG296700.028.3066358
GCAGAGT314900.026.5661479
GTACATG185450.024.5091361
TACATGG179850.024.146242
ACATGGG183700.023.0005663
AGAGTAC306450.022.78212710-11
AGTACTT213650.022.51470212-13
GAGTACT211300.022.04222312-13
CAGAGTA311600.021.53078810-11
Produced by FastQC (version 0.11.5)