##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431359_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3225750
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72574781058668	34.0	31.0	34.0	31.0	34.0
2	32.84582283189956	34.0	31.0	34.0	31.0	34.0
3	32.91872277764861	34.0	31.0	34.0	31.0	34.0
4	36.31424753933194	37.0	37.0	37.0	35.0	37.0
5	36.27405347593583	37.0	37.0	37.0	35.0	37.0
6	36.314061536076885	37.0	37.0	37.0	35.0	37.0
7	36.178676896845694	37.0	37.0	37.0	35.0	37.0
8	36.238562504843834	37.0	37.0	37.0	35.0	37.0
9	38.04802480043401	39.0	39.0	39.0	35.0	39.0
10-11	38.036695497171195	39.0	39.0	39.0	35.0	39.0
12-13	38.01319243586762	39.0	39.0	39.0	35.0	39.0
14-15	39.457873827791985	41.0	39.0	41.0	36.0	41.0
16-17	39.47606572115012	41.0	39.0	41.0	36.5	41.0
18-19	39.5649450515384	41.0	40.0	41.0	37.0	41.0
20-21	39.56540122452143	41.0	40.0	41.0	37.0	41.0
22-23	39.48362396341936	41.0	40.0	41.0	36.0	41.0
24-25	39.44421932883826	41.0	39.0	41.0	36.0	41.0
26-27	39.34622087886538	41.0	39.0	41.0	36.0	41.0
28-29	39.252161512826476	41.0	39.0	41.0	35.0	41.0
30-31	39.05041726730218	41.0	39.0	41.0	35.0	41.0
32-33	38.809295667674185	41.0	38.0	41.0	35.0	41.0
34-35	38.693295047663334	41.0	38.0	41.0	35.0	41.0
36-37	38.57557451755406	41.0	38.0	41.0	35.0	41.0
38-39	38.44887716035031	41.0	38.0	41.0	35.0	41.0
40-41	38.33815717275052	40.0	38.0	41.0	35.0	41.0
42-43	38.17393892893126	40.0	38.0	41.0	34.5	41.0
44-45	37.93540416957297	40.0	38.0	41.0	33.5	41.0
46-47	37.57461117569558	40.0	37.5	41.0	33.0	41.0
48-49	37.26134201348523	40.0	37.0	41.0	33.0	41.0
50-51	37.08846268309695	40.0	37.0	41.0	32.0	41.0
52-53	36.95905076338836	40.0	37.0	41.0	32.0	41.0
54-55	36.78913089979075	40.0	36.0	41.0	32.0	41.0
56-57	36.60555979229637	40.0	35.5	41.0	31.0	41.0
58-59	36.395438270169734	39.5	35.0	41.0	31.0	41.0
60-61	36.176343641013716	39.0	35.0	41.0	31.0	41.0
62-63	35.930837169650474	39.0	35.0	41.0	31.0	41.0
64-65	35.64824350926141	38.5	35.0	41.0	30.0	41.0
66-67	35.34559342788499	37.5	35.0	40.5	30.0	41.0
68-69	35.06559156785244	37.0	35.0	40.0	29.5	41.0
70-71	34.80304053320933	36.5	35.0	39.5	29.5	41.0
72-73	34.53420072851275	36.0	35.0	39.0	29.5	41.0
74-75	34.23708982407192	36.0	35.0	39.0	29.0	41.0
76-77	33.93087855537472	35.0	35.0	37.5	29.0	40.0
78-79	33.62943098504224	35.0	35.0	37.0	29.0	39.0
80-81	33.33989490816089	35.0	34.0	37.0	29.0	39.0
82-83	33.04548570099977	35.0	34.0	36.0	29.0	39.0
84-85	32.80648081841432	35.0	34.0	36.0	29.0	37.0
86-87	32.605550182128184	35.0	34.0	36.0	29.0	37.0
88-89	32.39852964426878	35.0	34.0	35.0	28.0	36.5
90-91	32.217248546849575	35.0	34.0	35.0	27.0	36.0
92-93	32.084686042005735	35.0	34.0	35.0	27.0	36.0
94-95	31.953466790668834	35.0	34.0	35.0	27.0	36.0
96-97	31.822938076416335	35.0	34.0	35.0	27.0	36.0
98-99	31.707245136789894	35.0	34.0	35.0	26.0	35.5
100	31.60239324188173	35.0	34.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19763780170876544
1101	2	0.20925717372266206
1101	3	0.1918245538288872
1101	4	0.09924415497714278
1101	5	0.13493818176350203
1101	6	0.16758073498784398
1101	7	0.16186870892754968
1101	8	0.1684716124948622
1101	9	0.24355356973769204
1101	10-11	0.26217409398766733
1101	12-13	0.21674453555150563
1101	14-15	0.29458592803855055
1101	16-17	0.3185689006975281
1101	18-19	0.2900382704435458
1101	20-21	0.2374948312103058
1101	22-23	0.23555043346130589
1101	24-25	0.24041024411478418
1101	26-27	0.18649195794603202
1101	28-29	0.21274571952414334
1101	30-31	0.2430188715288324
1101	32-33	0.22085942438819472
1101	34-35	0.19912269820535755
1101	36-37	0.1788931781780505
1101	38-39	0.216416486384027
1101	40-41	0.2313214589589947
1101	42-43	0.24059081557117423
1101	44-45	0.2709094791148985
1101	46-47	0.31204942865283414
1101	48-49	0.2980835189620663
1101	50-51	0.25187785885587743
1101	52-53	0.26917622851289735
1101	54-55	0.2644102667020718
1101	56-57	0.2878076929739706
1101	58-59	0.28203081383551165
1101	60-61	0.23986197336908077
1101	62-63	0.2732738908278023
1101	64-65	0.31217082305188626
1101	66-67	0.32573084325647983
1101	68-69	0.30626846808310404
1101	70-71	0.2663448128360102
1101	72-73	0.2278092290539533
1101	74-75	0.2602472624768666
1101	76-77	0.22504981139744018
1101	78-79	0.2369704131216892
1101	80-81	0.17790753127246717
1101	82-83	0.22204931117690307
1101	84-85	0.1986516296908718
1101	86-87	0.16779516497872748
1101	88-89	0.1955166690102459
1101	90-91	0.20065804262544873
1101	92-93	0.2315317351080921
1101	94-95	0.19936563267931717
1101	96-97	0.27702300517823986
1101	98-99	0.30855136816814266
1101	100	0.3743456530041236
1102	1	0.11465914702341706
1102	2	0.1463317071131911
1102	3	0.14684763520773458
1102	4	0.10219782840793101
1102	5	0.09078412042031658
1102	6	0.10921613110770068
1102	7	0.14112354390012172
1102	8	0.2029052764347199
1102	9	0.20716387820995408
1102	10-11	0.19809973031937034
1102	12-13	0.23340517890851942
1102	14-15	0.2592153087297362
1102	16-17	0.30332408693600854
1102	18-19	0.24885667019090363
1102	20-21	0.22960718057127139
1102	22-23	0.2333695344739013
1102	24-25	0.2681998316137708
1102	26-27	0.2377624259403035
1102	28-29	0.19183347098325498
1102	30-31	0.22673704227783276
1102	32-33	0.24466660853596522
1102	34-35	0.2179250820005194
1102	36-37	0.18086129108639426
1102	38-39	0.10115535480117188
1102	40-41	0.10211186213795287
1102	42-43	0.07627225454547926
1102	44-45	0.06524867279076574
1102	46-47	0.15208131912205403
1102	48-49	0.1653088581532174
1102	50-51	0.111532140584643
1102	52-53	0.08845561832348636
1102	54-55	0.14484417509259373
1102	56-57	0.13921139502954816
1102	58-59	0.11932419179763087
1102	60-61	0.11552334121204666
1102	62-63	0.13068489431681485
1102	64-65	0.13178775744622584
1102	66-67	0.13441409851196084
1102	68-69	0.08804446978201241
1102	70-71	0.09267163325468886
1102	72-73	0.09352263660346694
1102	74-75	0.013704719615667216
1102	76-77	0.03223285091148398
1102	78-79	-0.05156735176320382
1102	80-81	-0.02669562480105725
1102	82-83	-0.01449223319406201
1102	84-85	0.02001006404129413
1102	86-87	-0.011758657655633442
1102	88-89	0.022428915017975726
1102	90-91	0.08332609929747292
1102	92-93	0.07796314579535846
1102	94-95	0.055372891893291865
1102	96-97	-7.25523895770408E-4
1102	98-99	0.0057197828611883494
1102	100	0.05334909220134776
1103	1	0.15464185662965946
1103	2	0.18893085492744177
1103	3	0.15505953227987135
1103	4	0.14206661027233736
1103	5	0.1611093599441631
1103	6	0.16726156160169126
1103	7	0.12853450242813835
1103	8	0.17191188684364533
1103	9	0.25248273237246366
1103	10-11	0.24224379239090155
1103	12-13	0.20158694414686806
1103	14-15	0.23610446052079226
1103	16-17	0.24349002729382363
1103	18-19	0.20002368337262055
1103	20-21	0.2748628570951084
1103	22-23	0.28883541938098034
1103	24-25	0.23604064559550864
1103	26-27	0.2142821835378399
1103	28-29	0.2826322172893043
1103	30-31	0.31659930358596
1103	32-33	0.26214973308670153
1103	34-35	0.29577145041464803
1103	36-37	0.30983459258027324
1103	38-39	0.33726610192588424
1103	40-41	0.3027528629799079
1103	42-43	0.30830235018861885
1103	44-45	0.28933320540727436
1103	46-47	0.32526269229504834
1103	48-49	0.24901088370630475
1103	50-51	0.27162178107424495
1103	52-53	0.2576630140278624
1103	54-55	0.2005220560761103
1103	56-57	0.18432509086005666
1103	58-59	0.18098411119648716
1103	60-61	0.16415618751077687
1103	62-63	0.18949649017461212
1103	64-65	0.1752350455603846
1103	66-67	0.2424179617595712
1103	68-69	0.2877919620197389
1103	70-71	0.18427523147860114
1103	72-73	0.19624001343878916
1103	74-75	0.15965374862476267
1103	76-77	0.18438017825297237
1103	78-79	0.159018719371808
1103	80-81	0.15919750525731757
1103	82-83	0.18639328850867543
1103	84-85	0.19385711633386649
1103	86-87	0.2033445366473785
1103	88-89	0.21470244060268584
1103	90-91	0.30411585305963484
1103	92-93	0.27854265893547847
1103	94-95	0.32922154052619845
1103	96-97	0.30989287443097524
1103	98-99	0.38006062949770936
1103	100	0.43210839569362136
1104	1	0.09664390829009761
1104	2	0.12938448268360503
1104	3	0.15938989129470826
1104	4	0.05927457181586249
1104	5	0.06967774285780592
1104	6	0.10737487967604409
1104	7	0.07287307659988329
1104	8	0.13329317384099681
1104	9	0.19682883157644682
1104	10-11	0.17994961996894432
1104	12-13	0.23500694901932206
1104	14-15	0.29545520667972625
1104	16-17	0.2643092419091744
1104	18-19	0.23220020142039743
1104	20-21	0.271619865239316
1104	22-23	0.24075783986305055
1104	24-25	0.27174454726036856
1104	26-27	0.23122947202366362
1104	28-29	0.21561083941530512
1104	30-31	0.2229793873207555
1104	32-33	0.166098563065475
1104	34-35	0.17848493644574148
1104	36-37	0.17368049596015567
1104	38-39	0.15108816531051872
1104	40-41	0.15354590484002983
1104	42-43	0.14168684879052051
1104	44-45	0.19666696321183252
1104	46-47	0.314381866973271
1104	48-49	0.30620704481111005
1104	50-51	0.3101529413168862
1104	52-53	0.29604701533404665
1104	54-55	0.29709500292180735
1104	56-57	0.2682787319087865
1104	58-59	0.3041299766690102
1104	60-61	0.32238382755819117
1104	62-63	0.31404909111536483
1104	64-65	0.37352293735280284
1104	66-67	0.3945858715665125
1104	68-69	0.3927651221604975
1104	70-71	0.31832162597574154
1104	72-73	0.29881221797965907
1104	74-75	0.25968204670652995
1104	76-77	0.26593281419503256
1104	78-79	0.31648786587324196
1104	80-81	0.3285799164548777
1104	82-83	0.36086465128062173
1104	84-85	0.32299382334709037
1104	86-87	0.33753452559201946
1104	88-89	0.3970924146061705
1104	90-91	0.37457074967485227
1104	92-93	0.4475368378774718
1104	94-95	0.4462963387031067
1104	96-97	0.4309572516263849
1104	98-99	0.43711228027938276
1104	100	0.4462135240575513
1105	1	-0.011140758642270043
1105	2	0.05847868849810567
1105	3	0.058670885923319815
1105	4	-0.061898318047944656
1105	5	-0.032839428951561445
1105	6	-0.051023199756428994
1105	7	-0.3930928311777322
1105	8	-0.3900664551589088
1105	9	-0.30721521941072893
1105	10-11	-0.31139105489988594
1105	12-13	-0.16263203469284093
1105	14-15	-0.12298565399372308
1105	16-17	-0.08913987305101045
1105	18-19	-0.11220349961085674
1105	20-21	-0.0930075652313036
1105	22-23	-0.06037678512146982
1105	24-25	-0.09428498089367565
1105	26-27	-0.07809145278172025
1105	28-29	-0.12011540006945154
1105	30-31	-0.06769589795446507
1105	32-33	-0.03361833636252953
1105	34-35	-0.036333239337423606
1105	36-37	0.04327205550902136
1105	38-39	0.01765525582882077
1105	40-41	0.05264541210615192
1105	42-43	0.03037703934067082
1105	44-45	0.05211532225805371
1105	46-47	0.15789343379248066
1105	48-49	0.13397026444669535
1105	50-51	0.14973639714084186
1105	52-53	0.16252699684673644
1105	54-55	0.1879728492855719
1105	56-57	0.146924896803597
1105	58-59	0.19686902337071643
1105	60-61	0.18639799584411065
1105	62-63	0.15934300429290005
1105	64-65	0.206100044593569
1105	66-67	0.22017668870516616
1105	68-69	0.2168250632102513
1105	70-71	0.15609279557099143
1105	72-73	0.24304872886060025
1105	74-75	0.2494359823702581
1105	76-77	0.24806738550955743
1105	78-79	0.2290170436938439
1105	80-81	0.25543619704375686
1105	82-83	0.19225071844282837
1105	84-85	0.18621504094222985
1105	86-87	0.17360166068746707
1105	88-89	0.22064867586104242
1105	90-91	0.20943619940447888
1105	92-93	0.20450904515166712
1105	94-95	0.10022108115492045
1105	96-97	0.12008854641020505
1105	98-99	0.1403220413769546
1105	100	0.155157521911935
1106	1	0.08928251575422763
1106	2	0.09269789976476517
1106	3	0.10151759012675399
1106	4	0.04830460786484991
1106	5	0.08510708726058169
1106	6	0.10171210467625258
1106	7	0.037738801441214775
1106	8	0.0593449796829546
1106	9	0.13919070666139532
1106	10-11	0.1259116470512538
1106	12-13	0.12193587010005302
1106	14-15	0.09928281730941535
1106	16-17	0.1554596948895295
1106	18-19	0.17755661261669786
1106	20-21	0.1911966237948235
1106	22-23	0.19428796066602416
1106	24-25	0.1787120633104422
1106	26-27	0.14242443147946915
1106	28-29	0.152087183202525
1106	30-31	0.11798044758519666
1106	32-33	0.15660072087716514
1106	34-35	0.19752041152818123
1106	36-37	0.2191044640272679
1106	38-39	0.16015569911750305
1106	40-41	0.2154061886326275
1106	42-43	0.2103691673638295
1106	44-45	0.250799475442939
1106	46-47	0.2788072517865672
1106	48-49	0.241129267080602
1106	50-51	0.23807074540734163
1106	52-53	0.17536115523118667
1106	54-55	0.17425836224263236
1106	56-57	0.1442527365085624
1106	58-59	0.07590853612901327
1106	60-61	0.0991902349142535
1106	62-63	0.10516980742868043
1106	64-65	0.17567040390296995
1106	66-67	0.1739561483795029
1106	68-69	0.1539610332792094
1106	70-71	0.12206996868276576
1106	72-73	0.21209765617116716
1106	74-75	0.17510097879743114
1106	76-77	0.20801069067455558
1106	78-79	0.18465550275092113
1106	80-81	0.1279984840029087
1106	82-83	0.17943860343818585
1106	84-85	0.1627701310606895
1106	86-87	0.13873512113147513
1106	88-89	0.13615819959260023
1106	90-91	0.11931677547455166
1106	92-93	0.11612701308869333
1106	94-95	0.111909398832303
1106	96-97	0.09170149233036895
1106	98-99	0.12617106774438724
1106	100	0.06869614613295028
1107	1	-0.08142891080900228
1107	2	-0.018742501266274303
1107	3	0.007966605602511834
1107	4	-0.05715384486611441
1107	5	-0.11197288838654629
1107	6	-0.08352047704497068
1107	7	-0.038789410580392314
1107	8	0.017888298521818058
1107	9	-0.043131379730802166
1107	10-11	-0.006556202332987482
1107	12-13	0.027240449811053224
1107	14-15	0.020161012147710267
1107	16-17	0.05726219261239862
1107	18-19	0.016561683093151203
1107	20-21	-0.02459972190739279
1107	22-23	0.0032469417399312306
1107	24-25	-0.003107988531589001
1107	26-27	-0.02740887482044485
1107	28-29	-0.0018938254529317078
1107	30-31	-0.017307806422117267
1107	32-33	0.028296785618181275
1107	34-35	0.009887634420543634
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2216	36-37	-0.5768583867708799
2216	38-39	-0.576708257462279
2216	40-41	-0.5822884099558294
2216	42-43	-0.6144232451282434
2216	44-45	-0.49493190250988306
2216	46-47	-0.38395279019322714
2216	48-49	-0.39297088426078375
2216	50-51	-0.340625236827492
2216	52-53	-0.47715000883228953
2216	54-55	-0.44289641961002246
2216	56-57	-0.5017509910639575
2216	58-59	-0.5799285699251229
2216	60-61	-0.5446785733493513
2216	62-63	-0.6071109057088222
2216	64-65	-0.5505066282161195
2216	66-67	-0.4465286306993761
2216	68-69	-0.4591511653559621
2216	70-71	-0.5264424087071831
2216	72-73	-0.5247082294354968
2216	74-75	-0.4821176649047061
2216	76-77	-0.4330571663929845
2216	78-79	-0.4308436886351146
2216	80-81	-0.5000781015536404
2216	82-83	-0.5538037033744558
2216	84-85	-0.49806221414691976
2216	86-87	-0.4405986685510328
2216	88-89	-0.3874006913491357
2216	90-91	-0.4053185371922261
2216	92-93	-0.5052781422511572
2216	94-95	-0.46173311106652903
2216	96-97	-0.579340962741675
2216	98-99	-0.5456876251396459
2216	100	-0.5292141098821794
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40.0
3	428.0
4	239.0
5	354.0
6	472.0
7	820.0
8	1361.0
9	2188.0
10	2848.0
11	3914.0
12	5841.0
13	8456.0
14	9016.0
15	11968.0
16	15850.0
17	24909.0
18	30318.0
19	16388.0
20	10118.0
21	8900.0
22	8728.0
23	8964.0
24	9806.0
25	11829.0
26	14558.0
27	17762.0
28	21970.0
29	27260.0
30	34550.0
31	43737.0
32	54463.0
33	69966.0
34	98507.0
35	172951.0
36	293139.0
37	646585.0
38	1027124.0
39	503472.0
40	5951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.16805607615136	21.357904008437835	14.942530873785564	23.531509041625238
2	13.743877778463235	21.874206579619184	40.430641069717446	23.95127457220013
3	15.402574293030435	29.309305771698895	31.265260064357324	24.02285987091334
4	10.458166558730134	18.74046900774756	39.422617565826044	31.378746867696268
5	11.597302952801675	38.98786328760753	35.117073548787104	14.29776021080369
6	27.51419049833372	39.88452297915214	18.520282104936836	14.081004417577308
7	24.56362086336511	34.490676586840266	23.675734325350696	17.269968224443925
8	25.21584127722235	34.47055723475161	20.858932031310548	19.454669456715493
9	26.352352166162905	15.495807176625592	21.966488413547236	36.18535224366426
10-11	23.453801441525226	27.293141129969772	28.7686584515229	20.484398976982096
12-13	24.892490118577076	25.907091374099046	28.91941408974657	20.281004417577307
14-15	22.33916143532512	26.173928543749515	28.240161202821053	23.24674881810432
16-17	20.1139579942649	29.43608463148105	28.397178950631634	22.052778423622414
18-19	20.01672479268387	27.12803224056421	32.68745253041928	20.167790436332638
20-21	21.697124699682245	26.115151515151513	32.42523444160273	19.762489343563512
22-23	21.13335866078834	26.348334347826558	32.45476204980022	20.063544941584876
24-25	20.500442145580212	26.57977724542916	32.42978565433283	20.489994954657796
26-27	20.320906011702114	26.644551858146126	32.50089436786947	20.53364776228229
28-29	20.43283792153972	26.362387599225478	32.939603823501876	20.265170655732927
30-31	20.76441823004438	26.56281969725716	32.42192101702944	20.25084105566902
32-33	20.233103983641083	26.590753096592863	32.65912646226705	20.517016457499004
34-35	20.789524919100273	26.259177368041524	32.39520237797767	20.55609533488054
36-37	20.88433083833078	26.49441290556192	31.95182797379007	20.669428282317227
38-39	20.650754599176732	26.591955943634936	32.09712600668509	20.66016345050324
40-41	20.6326740156582	26.473745821782718	32.08312846557199	20.810451696987094
42-43	20.709579487382978	26.647532048057787	31.966557146628148	20.676331317931087
44-45	20.78705187718516	26.573130332507265	31.597707684514592	21.04211010579298
46-47	21.090494234103385	26.71460536217922	31.0464953160567	21.14840508766069
48-49	21.15185432391402	26.909746388122556	30.564886068877943	21.37351321908548
50-51	21.272970629060623	27.00317525763608	30.10023335857316	21.62362075473014
52-53	21.2095923178128	27.29534359162588	30.047402958941618	21.447661131619704
54-55	21.304237503958404	27.48517661807872	29.95635253585117	21.254233342111704
56-57	21.28734410682369	27.15563455240238	30.028956267681096	21.528065073092836
58-59	21.34730412227556	26.677709126661387	30.226951693577625	21.748035057485428
60-61	21.415194623981588	27.02542336889011	30.106413023024537	21.45296898410377
62-63	20.962210338680926	26.96760443307758	30.479252886925522	21.590932341315973
64-65	21.160536309385414	26.893730140277455	30.315554522204142	21.630179028132993
66-67	21.217049864580893	26.891011993732267	30.17377635704701	21.718161784639832
68-69	21.398710031027022	27.064795900099092	30.026584626487622	21.509909442386256
70-71	21.500138497263613	26.859044816846477	30.067686178066094	21.57313050782382
72-73	21.25971790711358	26.92501207472628	30.03429284264787	21.78097717551227
74-75	21.285712403499105	26.932904896870664	29.93647080889083	21.8449118907394
76-77	21.386959701327353	26.945840937534005	29.775797111917612	21.89140224922103
78-79	21.332160879730814	27.065186188236158	29.804285190813566	21.79836774121946
80-81	21.17429980502067	27.170855929664217	29.85321291188202	21.801631353433095
82-83	21.125094591548965	27.117478906200837	29.979545594754146	21.77788090749605
84-85	21.14325847094221	26.86091486284374	29.76746110982716	22.228365556386894
86-87	21.349603920217973	27.07142456348762	29.615337756712496	21.963633759581914
88-89	20.975680680235374	26.98003879459604	29.82694956485508	22.217330960313504
90-91	21.009844642147442	26.956714079425513	29.63942246584787	22.39401881257917
92-93	20.919104893214467	27.0662135103053	29.878279588959938	22.136402007520296
94-95	20.943547779580303	26.66069177135392	29.939371656365605	22.456388792700167
96-97	21.01260246487677	27.099135887224968	29.51234910732734	22.375912540570923
98-99	20.577857168543893	26.975244878902906	29.847945050897856	22.59895290165534
100	20.716543063825196	26.92030825376807	29.69063017918227	22.672518503224463
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4128.0
1	2603.0
2	3715.5
3	5222.5
4	2553.0
5	1469.5
6	3771.0
7	10484.5
8	17889.5
9	17951.0
10	10470.0
11	4409.0
12	3323.5
13	3451.5
14	3739.5
15	3995.5
16	4345.0
17	4888.0
18	5487.5
19	6051.5
20	6916.5
21	8126.0
22	9606.5
23	11835.0
24	15028.0
25	18737.5
26	23260.5
27	29365.0
28	36588.0
29	44421.0
30	51401.5
31	57895.5
32	65364.0
33	71546.0
34	76274.0
35	82494.5
36	89153.0
37	94032.5
38	100458.5
39	105812.5
40	109692.0
41	113913.0
42	116315.5
43	120191.5
44	121214.0
45	117439.5
46	114229.5
47	111696.5
48	106382.0
49	103552.0
50	105226.5
51	100958.0
52	91281.5
53	83837.5
54	78416.5
55	74015.5
56	66211.5
57	56878.0
58	52596.0
59	49515.5
60	43263.5
61	35264.0
62	29262.0
63	24382.0
64	19476.0
65	15640.5
66	12702.5
67	9695.5
68	7141.5
69	5245.0
70	3737.0
71	2742.5
72	2085.5
73	1534.0
74	1228.5
75	923.5
76	573.5
77	412.0
78	295.0
79	173.0
80	93.0
81	44.5
82	20.5
83	10.5
84	7.0
85	5.5
86	3.0
87	1.5
88	1.5
89	3.0
90	1.5
91	1.0
92	1.0
93	0.5
94	1.5
95	3.0
96	2.5
97	4.0
98	4.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008649151360148802
2	3.100054250949391E-5
3	3.1000542509493915E-4
4	0.0027590482833449587
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.650081376424087E-5
24-25	7.750135627373479E-5
26-27	2.480043400759513E-4
28-29	4.960086801519026E-4
30-31	0.0013020227853987444
32-33	0.0033480585910253434
34-35	0.004712082461443075
36-37	0.003162055335968379
38-39	0.0019840347206076104
40-41	0.00285204991087344
42-43	2.635046113306983E-4
44-45	4.960086801519026E-4
46-47	0.0031775556072231263
48-49	0.002263039603193056
50-51	8.525149190110827E-4
52-53	6.200108501898783E-5
54-55	5.735100364256374E-4
56-57	7.28512748973107E-4
58-59	6.045105789351313E-4
60-61	5.270092226613966E-4
62-63	0.0
64-65	0.0
66-67	7.28512748973107E-4
68-69	4.185073238781679E-4
70-71	0.0016895295667674185
72-73	1.2400217003797565E-4
74-75	0.0020925366193908393
76-77	3.4100596760443307E-4
78-79	0.001116019530341781
80-81	7.28512748973107E-4
82-83	0.0012090211578702627
84-85	9.765170890490583E-4
86-87	3.4100596760443307E-4
88-89	0.0013330233279082383
90-91	0.001116019530341781
92-93	0.002449042858250019
94-95	9.610168177943113E-4
96-97	0.002216538789428815
98-99	0.0022940401457025496
100	0.002976052080911416
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3225750.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.89861181811752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.94409991094004	33.98123902419863
2	16.179360581390778	15.499378239067783
3	5.615524967888842	8.069275517755635
4	2.5323498585771023	4.8518417145459765
5	1.3677484660082577	3.2756626419077617
6	0.7884915047006139	2.2660589103322857
7	0.5019699958285783	1.6830566182174604
8	0.36009003090080266	1.3798250087753194
9	0.2382259628873193	1.0269623629203282
>10	1.263446602972377	11.169140509671767
>50	0.11875454925903907	3.957354076466641
>100	0.08287085333462772	7.373969379517417
>500	0.00564040579945098	1.9169478659553403
>1k	0.0011021482594359333	0.9798699652223464
>5k	1.296645011101098E-4	0.3877681516045965
>10k+	1.9449675166516468E-4	2.1816500138407404
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30766	0.9537626908470898	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21621	0.670262729597768	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17867	0.5538866930171278	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7254	0.22487793536386888	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5233	0.16222583895218165	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4916	0.1523986669766721	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3834	0.11885607998139967	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3829	0.11870107726885219	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.100054250949391E-5	0.0
8	0.0	0.0	0.0	3.100054250949391E-5	0.0
9	0.0	0.0	0.0	3.100054250949391E-5	0.0
10-11	0.0	0.0	0.0	3.100054250949391E-5	0.0
12-13	0.0	0.0	0.0	3.100054250949391E-5	0.0
14-15	0.0	0.0	0.0	3.100054250949391E-5	0.0
16-17	0.0	0.0	0.0	3.100054250949391E-5	0.0
18-19	0.0	0.0	0.0	1.2400217003797565E-4	0.0
20-21	0.0	0.0	0.0	1.7050298380221653E-4	0.0
22-23	0.0	0.0	0.0	3.4100596760443307E-4	0.0
24-25	0.0	0.0	0.0	8.370146477563357E-4	0.0
26-27	0.0	0.0	0.0	0.0017360303805316593	0.0
28-29	0.0	0.0	0.0	0.0039990699837247155	0.0
30-31	0.0	0.0	0.0	0.009873672789273813	0.0
32-33	0.0	0.0	0.0	0.021080368906455864	0.0
34-35	0.0	0.0	0.0	0.03712314965511897	0.0
36-37	0.0	0.0	0.0	0.06086956521739131	0.0
38-39	0.0	0.0	0.0	0.09422614895760675	0.0
40-41	0.0	0.0	0.0	0.12772223513911493	0.0
42-43	0.0	0.0	0.0	0.15959079283887467	0.0
44-45	0.0	0.0	0.0	0.20119352088661552	0.0
46-47	0.0	0.0	0.0	0.250716887545532	0.0
48-49	0.0	0.0	0.0	0.3031698054715958	0.0
50-51	0.0	0.0	0.0	0.3596837944664032	0.0
52-53	0.0	0.0	0.0	0.41377974114547	0.0
54-55	0.0	0.0	0.0	0.47258777028598	0.0
56-57	0.0	0.0	0.0	0.5434395101914283	0.0
58-59	0.0	0.0	0.0	0.6229559017282802	0.0
60-61	0.0	0.0	0.0	0.7108269394714408	0.0
62-63	0.0	0.0	0.0	0.7994109896923196	0.0
64-65	0.0	0.0	0.0	0.8940866465163141	0.0
66-67	0.0	0.0	0.0	0.9997054948461599	0.0
68-69	0.0	0.0	0.0	1.123986669766721	0.0
70-71	0.0	0.0	0.0	1.2655971479500892	0.0
72-73	0.0	0.0	0.0	1.4130667286677516	0.0
74-75	0.0	0.0	0.0	1.5632798573975046	0.0
76-77	0.0	0.0	0.0	1.7255366968921955	0.0
78-79	0.0	0.0	0.0	1.9154460203053554	0.0
80-81	0.0	0.0	0.0	2.130946291560102	0.0
82-83	0.0	0.0	0.0	2.360582810199179	0.0
84-85	0.0	0.0	0.0	2.593226381461675	0.0
86-87	0.0	0.0	0.0	2.834844609780671	0.0
88	0.0	0.0	0.0	3.0330620785863753	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8455	0.0	63.20846	1
GTATCAA	17980	0.0	47.526142	1
GTGGTAT	2465	0.0	35.08563	1
ATCAACG	24405	0.0	34.76053	3
TCAACGC	24560	0.0	34.502342	4
TATCAAC	25080	0.0	33.974903	2
CAACGCA	25385	0.0	33.34401	5
AACGCAG	26140	0.0	32.43487	6
TATAACG	190	0.0	32.157166	2
TGGTATC	2775	0.0	30.655157	2
ACGCAGA	29510	0.0	28.571594	7
CGCAGAG	29670	0.0	28.306635	8
GCAGAGT	31490	0.0	26.566147	9
GTACATG	18545	0.0	24.509136	1
TACATGG	17985	0.0	24.14624	2
ACATGGG	18370	0.0	23.000566	3
AGAGTAC	30645	0.0	22.782127	10-11
AGTACTT	21365	0.0	22.514702	12-13
GAGTACT	21130	0.0	22.042223	12-13
CAGAGTA	31160	0.0	21.530788	10-11
>>END_MODULE