FastQCFastQC Report
Wed 25 May 2016
SRR2431358_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431358_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703491
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA117510.31729522226461465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37600.041.4998171
GTATCAA59950.031.4377251
TCAACGC60000.029.6882024
ATCAACG61050.029.1775933
CAACGCA62750.028.3122255
AACGCAG66000.026.846856
TATCAAC71250.025.5942732
ACGCAGA72250.023.7438377
CGCAGAG72900.023.2742448
GTACATG104400.022.1493261
TACATGG103800.020.7378652
ACATGGG104100.019.6396833
GCAGAGT87050.018.9510929
GTCTCGC8300.018.6866631
GTCGTAC1650.001572237617.0908341
CAGAGTA84950.017.04053710-11
AGAGTAC76350.016.62075210-11
TCTGTCG9300.016.171978
TACCTGG25600.016.1561782
GTACCTG28300.015.61123851
Produced by FastQC (version 0.11.5)