##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431358_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3703491 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.364436689599085 33.0 31.0 34.0 28.0 34.0 2 31.467737332154986 34.0 31.0 34.0 28.0 34.0 3 31.497680971818212 34.0 31.0 34.0 28.0 34.0 4 34.750513231974914 37.0 35.0 37.0 32.0 37.0 5 34.83582787159467 37.0 35.0 37.0 32.0 37.0 6 34.85371369877772 37.0 35.0 37.0 32.0 37.0 7 34.796706404848834 37.0 35.0 37.0 32.0 37.0 8 34.77746536983619 37.0 35.0 37.0 32.0 37.0 9 36.31057534634214 39.0 37.0 39.0 32.0 39.0 10-11 36.3347785913345 39.0 37.0 39.0 32.0 39.0 12-13 36.28499691237268 39.0 37.0 39.0 32.0 39.0 14-15 37.70283173362646 41.0 38.0 41.0 32.0 41.0 16-17 37.70969336769011 41.0 38.0 41.0 32.0 41.0 18-19 37.73628219968673 41.0 38.5 41.0 32.0 41.0 20-21 37.792820206664466 41.0 39.0 41.0 32.0 41.0 22-23 37.78533308167888 41.0 39.0 41.0 32.0 41.0 24-25 37.79526438703375 41.0 38.5 41.0 32.0 41.0 26-27 37.788806021129794 41.0 38.0 41.0 32.0 41.0 28-29 37.753286426239455 41.0 38.0 41.0 32.0 41.0 30-31 37.68775487776263 40.5 38.0 41.0 31.0 41.0 32-33 37.61650426583999 40.0 38.0 41.0 31.0 41.0 34-35 37.55012176349288 40.0 38.0 41.0 31.0 41.0 36-37 37.455355231050916 40.0 38.0 41.0 30.5 41.0 38-39 37.355809694150736 40.0 38.0 41.0 30.0 41.0 40-41 37.277379774920476 40.0 38.0 41.0 30.0 41.0 42-43 37.15991681902292 40.0 38.0 41.0 30.0 41.0 44-45 37.030544694181785 40.0 38.0 41.0 30.0 41.0 46-47 36.911946728100595 40.0 37.0 41.0 30.0 41.0 48-49 36.79276836368713 40.0 37.0 41.0 30.0 41.0 50-51 36.66674564620246 40.0 37.0 41.0 30.0 41.0 52-53 36.510242093203416 40.0 36.5 41.0 30.0 41.0 54-55 36.3565132195542 40.0 36.0 41.0 29.5 41.0 56-57 36.177514944683274 40.0 35.5 41.0 29.0 41.0 58-59 35.97224038076507 39.0 35.0 41.0 29.0 41.0 60-61 35.81096038845511 39.0 35.0 41.0 28.5 41.0 62-63 35.66083878697154 39.0 35.0 41.0 28.0 41.0 64-65 35.414147894513576 39.0 35.0 41.0 28.0 41.0 66-67 35.13385046703232 38.0 35.0 40.0 28.0 41.0 68-69 34.83069609187656 37.5 34.0 40.0 28.0 41.0 70-71 34.58241467307467 37.0 34.0 40.0 28.0 41.0 72-73 34.3390679226708 37.0 34.0 39.5 27.5 41.0 74-75 33.995479265374215 36.0 34.0 39.0 27.0 41.0 76-77 33.634897992191696 36.0 34.0 39.0 26.5 40.5 78-79 33.280553942212904 35.0 34.0 37.5 26.0 39.5 80-81 32.914114682606225 35.0 33.5 37.0 26.0 39.0 82-83 32.5585952551255 35.0 33.0 37.0 26.0 39.0 84-85 32.23875567673852 35.0 33.0 36.0 25.0 38.0 86-87 31.949697866148455 35.0 33.0 36.0 25.0 37.0 88-89 31.67486501249767 35.0 33.0 36.0 24.5 37.0 90-91 31.447116652909376 35.0 33.0 35.0 24.0 36.5 92-93 31.22059794393992 35.0 32.5 35.0 23.5 36.0 94-95 31.0001582290871 35.0 32.0 35.0 21.5 36.0 96-97 30.803842239659822 35.0 32.0 35.0 20.0 36.0 98-99 30.673817487338297 35.0 32.0 35.0 19.0 36.0 100 30.624853415331643 35.0 32.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30790869081527816 1101 2 0.29462635524288316 1101 3 0.31891796977942377 1101 4 0.3185719859443523 1101 5 0.2227637366261277 1101 6 0.28160431018253007 1101 7 0.25326700305069494 1101 8 0.27631211783972986 1101 9 0.3973589303633176 1101 10-11 0.30590608595521473 1101 12-13 0.3254438892839957 1101 14-15 0.3930915660329717 1101 16-17 0.4000899735587353 1101 18-19 0.37435347985138634 1101 20-21 0.3917620533705204 1101 22-23 0.3600568127859205 1101 24-25 0.47512956897890746 1101 26-27 0.3980774791723718 1101 28-29 0.4310611572258125 1101 30-31 0.47798059320867736 1101 32-33 0.4903939063397402 1101 34-35 0.5017635161850151 1101 36-37 0.510694490420704 1101 38-39 0.45926422588151894 1101 40-41 0.48500028237508275 1101 42-43 0.49608081545773786 1101 44-45 0.47358437365634387 1101 46-47 0.5290210866869387 1101 48-49 0.5309197125433229 1101 50-51 0.5135515074913855 1101 52-53 0.5317998564722544 1101 54-55 0.5428885412221689 1101 56-57 0.5993970210905459 1101 58-59 0.5924169189916242 1101 60-61 0.5691702199380373 1101 62-63 0.5976653076877056 1101 64-65 0.5622321465529367 1101 66-67 0.5644822342817335 1101 68-69 0.6071281998590692 1101 70-71 0.6330997056583811 1101 72-73 0.6617139771643537 1101 74-75 0.6347280389009811 1101 76-77 0.5863414373442666 1101 78-79 0.555413463721365 1101 80-81 0.47741360648740283 1101 82-83 0.4193056948913423 1101 84-85 0.5034940953657596 1101 86-87 0.5313291135928573 1101 88-89 0.5168194628489644 1101 90-91 0.4753062586977954 1101 92-93 0.4704547386158495 1101 94-95 0.43630601722809814 1101 96-97 0.4521303194356818 1101 98-99 0.5024647224542846 1101 100 0.5053451908358149 1102 1 0.09042955794251739 1102 2 0.17028417248778993 1102 3 0.16475760276550844 1102 4 0.1102253489392524 1102 5 0.15113525583058163 1102 6 0.15385546550916018 1102 7 0.21311908465953167 1102 8 0.16833741572581573 1102 9 0.22990322596643864 1102 10-11 0.2322727980627235 1102 12-13 0.21381619988635947 1102 14-15 0.27045468720012167 1102 16-17 0.22716916136468512 1102 18-19 0.14455062938805696 1102 20-21 0.15543448485099276 1102 22-23 0.08242754811553965 1102 24-25 0.13013901080588397 1102 26-27 0.0764684883020621 1102 28-29 0.08900011736140812 1102 30-31 0.18463999642089846 1102 32-33 0.1654328142784749 1102 34-35 0.18941017464563004 1102 36-37 0.18083442033957198 1102 38-39 0.19271727935353766 1102 40-41 0.18658666271944924 1102 42-43 0.19945101259252596 1102 44-45 0.216003401222288 1102 46-47 0.13575717362772366 1102 48-49 0.08993446861095578 1102 50-51 0.12589929154404444 1102 52-53 0.10890417650914941 1102 54-55 0.15480845042603875 1102 56-57 0.13465929279198008 1102 58-59 0.1313707152934711 1102 60-61 0.13731516700989488 1102 62-63 0.05463347040620192 1102 64-65 0.04867018256736344 1102 66-67 0.05590967271846381 1102 68-69 0.06033576582961331 1102 70-71 0.05803915413000027 1102 72-73 0.05874969782729522 1102 74-75 0.09897285832396108 1102 76-77 0.05538986875746588 1102 78-79 0.08043692354338816 1102 80-81 0.09365780828073866 1102 82-83 0.13185321287793528 1102 84-85 0.13898824001692844 1102 86-87 0.11815914602984989 1102 88-89 0.14216252794265927 1102 90-91 0.13429070488926342 1102 92-93 0.13492630866006294 1102 94-95 0.06379125927969298 1102 96-97 0.11951132781823048 1102 98-99 0.14522637811181838 1102 100 0.07925998552927283 1103 1 0.14622227617488193 1103 2 0.18120450498289742 1103 3 0.19236963405484175 1103 4 0.17906294619336904 1103 5 0.16802348283546564 1103 6 0.14669126476714922 1103 7 0.19295652994155432 1103 8 0.22735822831855046 1103 9 0.19340498385476934 1103 10-11 0.23787323221869627 1103 12-13 0.2132363140491904 1103 14-15 0.22678841587480747 1103 16-17 0.14386621272687705 1103 18-19 0.19149892183136785 1103 20-21 0.15879150755142035 1103 22-23 0.18423743802765813 1103 24-25 0.17857851118231594 1103 26-27 0.2116487954607038 1103 28-29 0.22505707160147637 1103 30-31 0.1536393301939114 1103 32-33 0.1871230385642022 1103 34-35 0.24861803542402328 1103 36-37 0.27098048856483814 1103 38-39 0.2378751232283136 1103 40-41 0.15923394657561118 1103 42-43 0.13483647478458494 1103 44-45 0.1267011646072831 1103 46-47 0.11033353318727279 1103 48-49 0.11347814716985027 1103 50-51 0.05761795298784733 1103 52-53 0.05588064650370228 1103 54-55 0.10341497425837787 1103 56-57 0.05807771834358988 1103 58-59 0.07735502774809078 1103 60-61 0.0487204319532637 1103 62-63 0.04980872106536083 1103 64-65 0.08666572170513831 1103 66-67 -0.007850131450474862 1103 68-69 0.07837340940459114 1103 70-71 0.010749544324916371 1103 72-73 -0.010323915587790111 1103 74-75 0.021201650122485205 1103 76-77 -3.507093373755765E-4 1103 78-79 -0.02413792504603407 1103 80-81 -0.00289296483717294 1103 82-83 -0.0071953820219263775 1103 84-85 -0.0069769082025317175 1103 86-87 0.0648713056349699 1103 88-89 0.059330153161393895 1103 90-91 0.005325465661563555 1103 92-93 0.07928246328189203 1103 94-95 0.07849609067963925 1103 96-97 0.0024022454000593996 1103 98-99 -0.048261807297638626 1103 100 -0.12819482902539292 1104 1 0.16813115363476072 1104 2 0.15436386421106008 1104 3 0.15396512481804336 1104 4 0.0690196356532553 1104 5 0.1410983539159858 1104 6 0.11439120289495008 1104 7 0.14258929956310595 1104 8 0.15275190591749777 1104 9 0.1796432798875145 1104 10-11 0.22031899683214817 1104 12-13 0.18518053071397134 1104 14-15 0.14886411096494356 1104 16-17 0.2229633446473258 1104 18-19 0.20614503514784843 1104 20-21 0.25981545815429996 1104 22-23 0.2091529355842212 1104 24-25 0.2285070036164072 1104 26-27 0.2485474571455768 1104 28-29 0.25574556851873353 1104 30-31 0.3383683762755467 1104 32-33 0.317203406663765 1104 34-35 0.3453944194571079 1104 36-37 0.26447847514508993 1104 38-39 0.269448386889664 1104 40-41 0.19835796316787935 1104 42-43 0.24684045359021667 1104 44-45 0.201579321547662 1104 46-47 0.16615046352558238 1104 48-49 0.1161536051785319 1104 50-51 0.1289857060205648 1104 52-53 0.12507615350691736 1104 54-55 0.08449849257929998 1104 56-57 0.1151137225888732 1104 58-59 0.14410495312404947 1104 60-61 0.1558167358612721 1104 62-63 0.0934737968959709 1104 64-65 0.07355276090878249 1104 66-67 0.11401508665188942 1104 68-69 0.1257434549109675 1104 70-71 0.10918709972111884 1104 72-73 0.11192178509486439 1104 74-75 0.12490620522019213 1104 76-77 0.14926593462757154 1104 78-79 0.11054025659748845 1104 80-81 0.160116337150626 1104 82-83 0.1729830266388106 1104 84-85 0.135897124115111 1104 86-87 0.16089930480695713 1104 88-89 0.2395098484436673 1104 90-91 0.18868790523324108 1104 92-93 0.19111546438095672 1104 94-95 0.19382627207611947 1104 96-97 0.13976446252916475 1104 98-99 0.17942917594426078 1104 100 0.21003996053478602 1105 1 0.1455658012257217 1105 2 0.23485748450061195 1105 3 0.23495076458205943 1105 4 0.20676782339476318 1105 5 0.12739328906997116 1105 6 0.16298218943914833 1105 7 0.13277758893615044 1105 8 0.20433564371670343 1105 9 0.16191844266353428 1105 10-11 0.14145801288566417 1105 12-13 0.27024369789483416 1105 14-15 0.21340128541650927 1105 16-17 0.22715766438327023 1105 18-19 0.26347511036014737 1105 20-21 0.27106894982384944 1105 22-23 0.2865551081561293 1105 24-25 0.2753013352862226 1105 26-27 0.26403698654350194 1105 28-29 0.2762417807417421 1105 30-31 0.3057741087828134 1105 32-33 0.325864585626519 1105 34-35 0.3059643428578127 1105 36-37 0.32597592496574634 1105 38-39 0.3544085413307485 1105 40-41 0.2822758179485234 1105 42-43 0.25660943487945787 1105 44-45 0.2663216907308765 1105 46-47 0.23220582976693294 1105 48-49 0.20790602670030722 1105 50-51 0.21036389222771135 1105 52-53 0.2735720880434087 1105 54-55 0.26708467292181126 1105 56-57 0.2635722554100539 1105 58-59 0.30285123317941753 1105 60-61 0.31753888131756725 1105 62-63 0.28600215140542673 1105 64-65 0.27640467511086797 1105 66-67 0.21701276460314745 1105 68-69 0.2331825820390847 1105 70-71 0.29199649841278585 1105 72-73 0.33392281767838483 1105 74-75 0.2852830266891502 1105 76-77 0.32565473562574 1105 78-79 0.26911570960069753 1105 80-81 0.2280036289326901 1105 82-83 0.2646467567922386 1105 84-85 0.3205052726106459 1105 86-87 0.34679839942177537 1105 88-89 0.3271940071936683 1105 90-91 0.3280283838653908 1105 92-93 0.3725582466553661 1105 94-95 0.3685683140168976 1105 96-97 0.3987445403276233 1105 98-99 0.3743261371984694 1105 100 0.41126285034120613 1106 1 -0.011005741508935074 1106 2 0.05105048733208406 1106 3 -0.01106170002200102 1106 4 -0.03833692831394586 1106 5 -0.05233742333601299 1106 6 -0.03479105819177164 1106 7 -0.0844407271162666 1106 8 -0.057657570960770954 1106 9 -0.07373370917260758 1106 10-11 -0.05488634706285467 1106 12-13 -0.07627516603599105 1106 14-15 -0.09196878596838332 1106 16-17 -0.04972144520051813 1106 18-19 -0.05578907985260173 1106 20-21 -0.02308349991119485 1106 22-23 -0.03564658185959502 1106 24-25 0.007966854422647884 1106 26-27 0.022045386014731605 1106 28-29 0.05230178273688324 1106 30-31 0.03926005746922101 1106 32-33 0.028012966025571018 1106 34-35 0.08702074860964615 1106 36-37 0.07179214610263074 1106 38-39 0.03100473084835187 1106 40-41 0.062353802821149884 1106 42-43 0.03564634129465816 1106 44-45 0.02343951195960159 1106 46-47 0.0774112742545725 1106 48-49 0.050813122748145645 1106 50-51 0.06953033322880486 1106 52-53 0.061050276952293814 1106 54-55 0.03641645095067503 1106 56-57 0.00487274132576232 1106 58-59 0.04612381425369705 1106 60-61 0.01578250991300223 1106 62-63 0.11455584874658342 1106 64-65 0.026300960463885303 1106 66-67 0.08576889964780321 1106 68-69 0.10056833466506987 1106 70-71 0.09529680203341684 1106 72-73 0.10527882359792784 1106 74-75 0.08674400752548905 1106 76-77 0.11022130466725599 1106 78-79 0.11587450951605405 1106 80-81 0.07382634462840088 1106 82-83 0.11319195019331829 1106 84-85 0.07819029999846805 1106 86-87 0.14515853348763486 1106 88-89 0.07705331152540751 1106 90-91 0.04782289751564406 1106 92-93 0.05740427154641026 1106 94-95 0.07954588782394723 1106 96-97 0.04822381127882025 1106 98-99 0.1232028683365094 1106 100 0.1506474922484884 1107 1 0.05986984330741052 1107 2 0.07846506364625583 1107 3 0.09597690710943496 1107 4 0.13527821505441295 1107 5 0.08043416473728371 1107 6 0.11385807748440868 1107 7 0.10213150737210697 1107 8 0.12630097207522084 1107 9 0.07605893926653806 1107 10-11 0.0941622323590181 1107 12-13 0.10544343408159307 1107 14-15 0.16408598578715328 1107 16-17 0.15183441958745902 1107 18-19 0.12822047439766493 1107 20-21 0.09121904042604712 1107 22-23 0.12880428992706072 1107 24-25 0.1139618476484614 1107 26-27 0.1642242277219168 1107 28-29 0.1403142838972613 1107 30-31 0.10109685859435302 1107 32-33 0.08343812925235738 1107 34-35 0.1482369360469633 1107 36-37 0.1548976050761368 1107 38-39 0.1166744020649233 1107 40-41 0.08926832361910186 1107 42-43 0.07757255386899686 1107 44-45 0.12428577025745824 1107 46-47 0.16992190879585678 1107 48-49 0.13655008083552644 1107 50-51 0.0923791404188492 1107 52-53 0.16411895870602677 1107 54-55 0.07761544656215591 1107 56-57 0.10119873559697368 1107 58-59 0.0463688288669033 1107 60-61 0.06969241406713422 1107 62-63 0.0834060926842426 1107 64-65 0.14742909857125142 1107 66-67 0.1880853821401729 1107 68-69 0.2833593382466262 1107 70-71 0.24834925363570903 1107 72-73 0.22823641830768793 1107 74-75 0.171886411177546 1107 76-77 0.22445821148556178 1107 78-79 0.19677720323580417 1107 80-81 0.19439612002130247 1107 82-83 0.20473088405136508 1107 84-85 0.15735131644886025 1107 86-87 0.09606605681506508 1107 88-89 0.18023872902254112 1107 90-91 0.10923269397437352 1107 92-93 0.16175076677413358 1107 94-95 0.20433102792200586 1107 96-97 0.1840683737533766 1107 98-99 0.21042273137223333 1107 100 0.18214727352065196 1108 1 -0.012385862954847227 1108 2 -0.019531712302736537 1108 3 -0.04287804139815776 1108 4 -0.048300173536141244 1108 5 -0.04677466957318899 1108 6 -0.04087994270184936 1108 7 -0.026859282520391048 1108 8 0.03529237802842289 1108 9 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-0.2508533004287621 2214 96-97 -0.24801026037869534 2214 98-99 -0.20899825169014363 2214 100 -0.24365093975799113 2215 1 -0.26531880703904065 2215 2 -0.35246323843590943 2215 3 -0.29801055071658666 2215 4 -0.2384953959008982 2215 5 -0.24801644317201266 2215 6 -0.2583111965623104 2215 7 -0.285378523778391 2215 8 -0.3572942138354307 2215 9 -0.34150689959651004 2215 10-11 -0.31793598231191567 2215 12-13 -0.32038629307110966 2215 14-15 -0.3628569833471147 2215 16-17 -0.31888632396887573 2215 18-19 -0.3491321539794896 2215 20-21 -0.4015766520037829 2215 22-23 -0.3143986348544061 2215 24-25 -0.3512685667998525 2215 26-27 -0.35315619664968523 2215 28-29 -0.3512857601103647 2215 30-31 -0.3894487820617982 2215 32-33 -0.40053851003179375 2215 34-35 -0.44198247649434563 2215 36-37 -0.4299724537732743 2215 38-39 -0.45184919594696993 2215 40-41 -0.4723129632157139 2215 42-43 -0.3363482021993107 2215 44-45 -0.33688219003212794 2215 46-47 -0.3455455859620571 2215 48-49 -0.35903885556050597 2215 50-51 -0.3495738275073492 2215 52-53 -0.26243996837074945 2215 54-55 -0.303961644447341 2215 56-57 -0.2867947862213214 2215 58-59 -0.24782022272557214 2215 60-61 -0.2578277776680622 2215 62-63 -0.3465438486389232 2215 64-65 -0.28107484701460805 2215 66-67 -0.2978377317878085 2215 68-69 -0.3289335670334239 2215 70-71 -0.3771299225587512 2215 72-73 -0.3652535268122108 2215 74-75 -0.3282852509301577 2215 76-77 -0.26151112123799436 2215 78-79 -0.2565136612983565 2215 80-81 -0.25470819531491884 2215 82-83 -0.24260228605105993 2215 84-85 -0.21693808979331664 2215 86-87 -0.3031666141027962 2215 88-89 -0.30732350725527624 2215 90-91 -0.30547432092781435 2215 92-93 -0.31077326484232515 2215 94-95 -0.2824518526792552 2215 96-97 -0.27730781380541103 2215 98-99 -0.23216527309756074 2215 100 -0.22586858648331898 2216 1 -0.412144331963038 2216 2 -0.4526889049465055 2216 3 -0.5563392197014885 2216 4 -0.452335327065029 2216 5 -0.3547869110066273 2216 6 -0.3819313541078131 2216 7 -0.354111744755663 2216 8 -0.4173485888164876 2216 9 -0.39413373244684635 2216 10-11 -0.42929891521679053 2216 12-13 -0.4412746137859074 2216 14-15 -0.47121525948251985 2216 16-17 -0.5316517356872197 2216 18-19 -0.5953022771295124 2216 20-21 -0.6491003367885213 2216 22-23 -0.6322188575791543 2216 24-25 -0.6616673175965815 2216 26-27 -0.7538005691343486 2216 28-29 -0.6445679895884524 2216 30-31 -0.6709489820159078 2216 32-33 -0.765632021095044 2216 34-35 -0.7732428882370144 2216 36-37 -0.7259636895701718 2216 38-39 -0.7403145499901953 2216 40-41 -0.7587832907549625 2216 42-43 -0.7823022284723677 2216 44-45 -0.7624868032739016 2216 46-47 -0.6053190801494424 2216 48-49 -0.759564923420271 2216 50-51 -0.6349162786360267 2216 52-53 -0.6957438793497133 2216 54-55 -0.792310153521349 2216 56-57 -0.6853448452063802 2216 58-59 -0.6971823344805514 2216 60-61 -0.6572007292378075 2216 62-63 -0.7542354925405732 2216 64-65 -0.7612614597825527 2216 66-67 -0.7941997622324877 2216 68-69 -0.903508802552615 2216 70-71 -0.8891941287886453 2216 72-73 -0.8618684940653978 2216 74-75 -0.8821749315574934 2216 76-77 -0.7471520392042237 2216 78-79 -0.6853306983245844 2216 80-81 -0.6747901287823126 2216 82-83 -0.6624790139184711 2216 84-85 -0.6356010227356137 2216 86-87 -0.6276687966623058 2216 88-89 -0.7119486695484021 2216 90-91 -0.6977186205981205 2216 92-93 -0.7841722330903238 2216 94-95 -0.8750126543558387 2216 96-97 -0.932604979116654 2216 98-99 -0.9372091062768817 2216 100 -0.8713542457405481 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62986.0 3 12178.0 4 7937.0 5 7372.0 6 5457.0 7 5214.0 8 5871.0 9 6269.0 10 6502.0 11 7005.0 12 7943.0 13 8558.0 14 9177.0 15 9320.0 16 9027.0 17 9162.0 18 9128.0 19 8832.0 20 8820.0 21 9403.0 22 11043.0 23 16691.0 24 26700.0 25 35483.0 26 35889.0 27 28666.0 28 28717.0 29 35380.0 30 42597.0 31 50924.0 32 65728.0 33 88160.0 34 124812.0 35 193127.0 36 333923.0 37 667975.0 38 1118804.0 39 580812.0 40 1899.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.72755987749893 21.461501863923587 13.869341197652584 22.94159706092489 2 15.21373083226902 22.17073625148171 40.40691347890773 22.208619437341532 3 16.986648543225837 28.187620815063408 30.92306691173274 23.902663729978013 4 11.995985407274059 19.225438498669767 40.24126978640952 28.537306307646652 5 11.930419163972587 39.07416002901047 35.873909238607574 13.121511568409375 6 27.584730191054874 40.93872511098313 18.92201169113142 12.554533006830582 7 24.196467603134447 34.316108774126896 24.45943570539256 17.027987917346092 8 22.950589052329278 37.38942527469353 22.159173601339923 17.500812071637274 9 23.923238911610696 18.41405851938077 22.43928768829194 35.2234148807166 10-11 21.83677508599319 28.667519375637745 30.405717200338817 19.089988338030253 12-13 22.9440546770601 26.730104109879033 29.619823566467424 20.706017646593445 14-15 20.852798076193515 28.59025443831239 26.903967094830257 23.652980390663835 16-17 20.25587479488947 31.3922863590056 27.310448439053854 21.04139040705108 18-19 20.15147329911157 30.372640300732474 29.168357638779195 20.307528761376766 20-21 21.737193367015067 29.75267119590678 28.327461846133822 20.18267359094433 22-23 21.24568413964014 29.882197634610158 28.2894571635249 20.5826610622248 24-25 21.12454168242882 29.78126583809708 28.346889461861796 20.7473030176123 26-27 20.90018579767036 29.485247837783323 28.506050102457387 21.10851626208893 28-29 20.997728899570703 29.518797264526903 28.567897154333572 20.915576681568822 30-31 21.26162855532793 30.06530865067581 28.083597340995293 20.589465453000965 32-33 20.654957714221528 29.73344609180905 28.67888702848205 20.932709165487374 34-35 20.861182057685575 29.263956629029202 28.695925006973148 21.17893630631207 36-37 21.378005238840867 29.757099450221425 28.070852609065337 20.794042701872367 38-39 21.17327948144062 29.700801217013893 28.342960736235085 20.782958565310405 40-41 20.901697884509506 29.414395768748996 28.61926760453853 21.064638742202966 42-43 20.78054192652284 29.496615490627626 28.579278307953228 21.143564274896306 44-45 21.085537942444034 29.569317165884833 28.48038512851793 20.8647597631532 46-47 21.01469937418506 29.877715377194114 28.239274781550705 20.868310467070124 48-49 20.840755384581737 29.471288036071915 28.582680503341308 21.105276076005044 50-51 20.83260091627062 29.294130861935404 28.589241880161175 21.2840263416328 52-53 20.847708283886742 29.40485072057688 28.49896219539888 21.24847880013749 54-55 21.088656621549777 29.857666725800065 28.22743460156917 20.826242051080992 56-57 20.82776763869549 29.512424898561925 28.31718775609283 21.342619706649753 58-59 20.71045940168344 29.084963349445157 28.718889825842698 21.485687423028704 60-61 21.151178712193442 29.588879789366302 28.203389720671655 21.056551777768597 62-63 20.97859046466981 29.51938576855885 28.27530248495338 21.226721281817962 64-65 20.94486834646082 29.197922661857152 28.583961153393307 21.27324783828873 66-67 20.811823223007696 29.31916129943343 28.276509919964703 21.592505557594173 68-69 21.309218788435018 29.453196997103543 28.199271984190048 21.038312230271384 70-71 21.30063229531272 29.12898937785997 28.415945927774633 21.154432399052677 72-73 20.954404911474068 29.295453937919653 28.671448101264456 21.078693049341823 74-75 20.960817779765094 29.238291655089753 28.56045822711598 21.240432338029173 76-77 21.056835294050938 29.325844183231442 28.30178337141902 21.3155371512986 78-79 21.199659996473596 29.424791365768137 28.344094801364445 21.031453836393823 80-81 20.97827428229203 29.17564805746794 28.58394957622416 21.262128084015867 82-83 20.879743992586445 29.23788165556969 28.61742576893422 21.264948582909646 84-85 20.93886839609026 29.185676996351255 28.483335488969935 21.392119118588546 86-87 21.16671907294752 29.297505168510384 28.376765741764437 21.15901001677766 88-89 20.885568710915443 29.165101641784236 28.501351074392396 21.447978572907928 90-91 20.793450311978308 29.082021638326584 28.526475458584144 21.598052591110964 92-93 20.983887689468936 29.31802996184193 28.338270899215733 21.359811449473398 94-95 20.824152590427616 29.051812872697656 28.567083148368365 21.556951388506356 96-97 20.793943423801696 29.107438072318438 28.58749869208467 21.5111198117952 98-99 20.74698617225819 29.11900817319058 28.576694019544536 21.5573116350067 100 20.945498397535857 29.159770899877653 28.402782446962338 21.491948255624152 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9061.0 1 6411.5 2 3170.0 3 2122.0 4 1391.5 5 1024.5 6 927.5 7 984.5 8 1108.5 9 1155.5 10 998.5 11 824.5 12 818.5 13 830.5 14 919.5 15 1134.0 16 1363.0 17 1704.5 18 2240.5 19 3072.0 20 4477.0 21 6350.0 22 9058.5 23 13388.0 24 18970.0 25 25792.5 26 34872.5 27 45531.0 28 57779.5 29 71702.0 30 84107.0 31 95195.5 32 105615.5 33 113712.5 34 120300.0 35 126073.5 36 130080.5 37 133486.0 38 136003.0 39 136005.0 40 135799.0 41 134580.0 42 130986.0 43 127815.5 44 123832.5 45 118977.5 46 115288.5 47 111280.5 48 107466.5 49 104321.5 50 100807.5 51 95509.5 52 88574.0 53 82227.0 54 77139.0 55 73068.0 56 67782.5 57 61104.5 58 56118.0 59 51220.0 60 44499.0 61 37365.5 62 31216.0 63 25716.0 64 20612.5 65 16507.0 66 13053.0 67 10140.5 68 7858.5 69 6058.5 70 4631.0 71 3589.0 72 2842.5 73 2234.5 74 1767.5 75 1399.5 76 1046.0 77 803.5 78 629.0 79 479.0 80 374.0 81 250.5 82 189.5 83 174.0 84 139.0 85 118.5 86 95.0 87 77.0 88 72.5 89 67.0 90 56.5 91 41.5 92 37.0 93 34.0 94 26.0 95 17.5 96 13.0 97 13.5 98 13.0 99 19.5 100 50.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.5102015908773643E-4 2 2.7001550699056646E-5 3 0.0 4 5.400310139811329E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.7001550699056646E-5 64-65 4.0502326048584974E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.2150697814575492E-4 84-85 5.940341153792462E-4 86-87 0.0014715845130985872 88-89 0.003834220199266044 90-91 0.002929668250847646 92-93 0.0021736248312740603 94-95 0.0023491349108179286 96-97 0.0021196217298759465 98-99 0.002767658946653306 100 0.0035642046922754775 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3703491.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.85644945981954 #Duplication Level Percentage of deduplicated Percentage of total 1 81.52222796140273 59.39420081321827 2 12.593342008604653 18.350123711602546 3 3.121941397096808 6.823606968423062 4 1.1076511819924433 3.2279812943976727 5 0.5492669350609596 2.0008819347109394 6 0.2993632104782696 1.3086324368603621 7 0.17549481896035152 0.8950150585631521 8 0.12388243888291856 0.7220507717946032 9 0.09121273656927484 0.5980892518756087 >10 0.39426144408880937 4.919957210634691 >50 0.015795297975956704 0.7842026706526102 >100 0.005337787598602516 0.5661542091549805 >500 1.856510781055142E-4 0.09121852093984874 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 3.7130210162828375E-5 0.31788514717167016 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11751 0.31729522226461465 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.7001550699056646E-5 0.0 5 0.0 0.0 0.0 2.7001550699056646E-5 2.7001550699056646E-5 6 0.0 0.0 0.0 2.7001550699056646E-5 2.7001550699056646E-5 7 0.0 0.0 0.0 2.7001550699056646E-5 2.7001550699056646E-5 8 0.0 0.0 0.0 5.400310139811329E-5 2.7001550699056646E-5 9 0.0 0.0 0.0 1.0800620279622658E-4 2.7001550699056646E-5 10-11 0.0 0.0 0.0 1.3500775349528323E-4 2.7001550699056646E-5 12-13 0.0 0.0 0.0 1.6200930419433987E-4 2.7001550699056646E-5 14-15 0.0 0.0 0.0 1.890108548933965E-4 2.7001550699056646E-5 16-17 0.0 0.0 0.0 2.7001550699056647E-4 2.7001550699056646E-5 18-19 1.3500775349528323E-5 0.0 0.0 2.835162823400948E-4 2.7001550699056646E-5 20-21 2.7001550699056646E-5 0.0 0.0 3.105178330391514E-4 2.7001550699056646E-5 22-23 2.7001550699056646E-5 0.0 0.0 5.80533340029718E-4 2.7001550699056646E-5 24-25 2.7001550699056646E-5 0.0 0.0 7.425426442240578E-4 2.7001550699056646E-5 26-27 2.7001550699056646E-5 0.0 0.0 0.0010395597019136808 2.7001550699056646E-5 28-29 2.7001550699056646E-5 0.0 0.0 0.0022276279326721733 2.7001550699056646E-5 30-31 2.7001550699056646E-5 0.0 0.0 0.005130294632820763 2.7001550699056646E-5 32-33 2.7001550699056646E-5 0.0 0.0 0.012663727277857566 2.7001550699056646E-5 34-35 2.7001550699056646E-5 0.0 0.0 0.025259950678967493 2.7001550699056646E-5 36-37 2.7001550699056646E-5 0.0 0.0 0.04360750437897648 2.7001550699056646E-5 38-39 2.7001550699056646E-5 0.0 0.0 0.06766588605183596 5.400310139811329E-5 40-41 2.7001550699056646E-5 0.0 0.0 0.09316885068709496 5.400310139811329E-5 42-43 2.7001550699056646E-5 0.0 0.0 0.11764575639578981 5.400310139811329E-5 44-45 2.7001550699056646E-5 0.0 0.0 0.15049314282119222 5.400310139811329E-5 46-47 2.7001550699056646E-5 0.0 0.0 0.18882184403850313 5.400310139811329E-5 48-49 2.7001550699056646E-5 2.7001550699056646E-5 0.0 0.2283656150372716 5.400310139811329E-5 50-51 2.7001550699056646E-5 2.7001550699056646E-5 0.0 0.26836841239792397 5.400310139811329E-5 52-53 2.7001550699056646E-5 2.7001550699056646E-5 0.0 0.3100588066772675 5.400310139811329E-5 54-55 2.7001550699056646E-5 2.7001550699056646E-5 0.0 0.3574195266034128 5.400310139811329E-5 56-57 2.7001550699056646E-5 2.7001550699056646E-5 0.0 0.41392027144118887 5.400310139811329E-5 58-59 2.7001550699056646E-5 2.7001550699056646E-5 0.0 0.477373915583972 5.400310139811329E-5 60-61 2.7001550699056646E-5 2.7001550699056646E-5 0.0 0.5454448248962938 5.400310139811329E-5 62-63 2.7001550699056646E-5 2.7001550699056646E-5 0.0 0.613272720252324 5.400310139811329E-5 64-65 2.7001550699056646E-5 2.7001550699056646E-5 0.0 0.6895115986511104 5.400310139811329E-5 66-67 2.7001550699056646E-5 2.7001550699056646E-5 0.0 0.7786707190593956 5.400310139811329E-5 68-69 2.7001550699056646E-5 2.7001550699056646E-5 0.0 0.8800345403836543 5.400310139811329E-5 70-71 2.7001550699056646E-5 2.7001550699056646E-5 0.0 0.9938865789062266 5.400310139811329E-5 72-73 2.7001550699056646E-5 2.7001550699056646E-5 0.0 1.1134359446262998 5.400310139811329E-5 74-75 5.400310139811329E-5 2.7001550699056646E-5 0.0 1.2374945693131156 5.400310139811329E-5 76-77 8.100465209716993E-5 2.7001550699056646E-5 0.0 1.374554440661527 5.400310139811329E-5 78-79 8.100465209716993E-5 4.050232604858497E-5 0.0 1.5330805448156886 5.400310139811329E-5 80-81 8.100465209716993E-5 5.400310139811329E-5 0.0 1.7102377189521993 5.400310139811329E-5 82-83 8.100465209716993E-5 6.750387674764162E-5 0.0 1.9012466885973263 5.400310139811329E-5 84-85 8.100465209716993E-5 8.100465209716993E-5 0.0 2.0971564396943316 5.400310139811329E-5 86-87 8.100465209716993E-5 8.100465209716993E-5 0.0 2.3079440452265176 5.400310139811329E-5 88 8.100465209716993E-5 8.100465209716993E-5 0.0 2.4821985526628794 5.400310139811329E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3760 0.0 41.499817 1 GTATCAA 5995 0.0 31.437725 1 TCAACGC 6000 0.0 29.688202 4 ATCAACG 6105 0.0 29.177593 3 CAACGCA 6275 0.0 28.312225 5 AACGCAG 6600 0.0 26.84685 6 TATCAAC 7125 0.0 25.594273 2 ACGCAGA 7225 0.0 23.743837 7 CGCAGAG 7290 0.0 23.274244 8 GTACATG 10440 0.0 22.149326 1 TACATGG 10380 0.0 20.737865 2 ACATGGG 10410 0.0 19.639683 3 GCAGAGT 8705 0.0 18.951092 9 GTCTCGC 830 0.0 18.686663 1 GTCGTAC 165 0.0015722376 17.090834 1 CAGAGTA 8495 0.0 17.040537 10-11 AGAGTAC 7635 0.0 16.620752 10-11 TCTGTCG 930 0.0 16.17197 8 TACCTGG 2560 0.0 16.156178 2 GTACCTG 2830 0.0 15.6112385 1 >>END_MODULE