FastQCFastQC Report
Wed 25 May 2016
SRR2431358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431358_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703491
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140930.3805328540018053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109460.2955589739518741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80100.21628242109944373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61050.059.5152661
GTATCAA123200.043.8754581
ATCAACG154800.034.5513343
TCAACGC155600.034.43414
TATCAAC161500.033.4671552
CAACGCA161300.033.1876875
AACGCAG166700.032.1408126
ACGCAGA187350.028.447697
CGCAGAG189450.028.181978
GTGGTAT24700.025.6904281
GCAGAGT207850.025.5288689
TGGTATC25150.024.2939912
GTACATG94850.023.8364891
TACATGG94700.023.077912
AGAGTAC197050.022.55135710-11
ACATGGG93550.022.4572813
GAGTACT158000.021.55107512-13
CAGAGTA206800.021.12450210-11
AGTACTT166150.020.4515212-13
TCTGTCG9650.018.5073368
Produced by FastQC (version 0.11.5)