##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431358_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3703491
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7674540048835	34.0	31.0	34.0	31.0	34.0
2	32.899150018185544	34.0	31.0	34.0	31.0	34.0
3	32.96904704237164	34.0	31.0	34.0	31.0	34.0
4	36.35515517656179	37.0	37.0	37.0	35.0	37.0
5	36.30463554521936	37.0	37.0	37.0	35.0	37.0
6	36.33616525597065	37.0	37.0	37.0	35.0	37.0
7	36.203358668888356	37.0	37.0	37.0	35.0	37.0
8	36.25721974212979	37.0	37.0	37.0	35.0	37.0
9	38.08015896352928	39.0	39.0	39.0	35.0	39.0
10-11	38.07366819576448	39.0	39.0	39.0	35.0	39.0
12-13	38.05469299101848	39.0	39.0	39.0	35.0	39.0
14-15	39.56554518425993	41.0	40.0	41.0	37.0	41.0
16-17	39.57763850377927	41.0	40.0	41.0	37.0	41.0
18-19	39.63982348006246	41.0	40.0	41.0	37.0	41.0
20-21	39.64267929907214	41.0	40.0	41.0	37.0	41.0
22-23	39.57291309739918	41.0	40.0	41.0	37.0	41.0
24-25	39.547528264548234	41.0	40.0	41.0	36.5	41.0
26-27	39.46483439543933	41.0	40.0	41.0	36.0	41.0
28-29	39.375804207435635	41.0	39.0	41.0	36.0	41.0
30-31	39.22869233380073	41.0	39.0	41.0	35.5	41.0
32-33	39.042212604269864	41.0	39.0	41.0	35.0	41.0
34-35	38.93795975202856	41.0	39.0	41.0	35.0	41.0
36-37	38.82271537854419	41.0	39.0	41.0	35.0	41.0
38-39	38.69760288333359	41.0	39.0	41.0	35.0	41.0
40-41	38.605129322577	41.0	39.0	41.0	35.0	41.0
42-43	38.49086902060785	41.0	38.0	41.0	35.0	41.0
44-45	38.321911272364375	41.0	38.0	41.0	34.5	41.0
46-47	38.076223352507135	41.0	38.0	41.0	34.0	41.0
48-49	37.86136121297446	40.5	38.0	41.0	33.0	41.0
50-51	37.73624534257002	40.0	38.0	41.0	33.0	41.0
52-53	37.63470844130578	40.0	38.0	41.0	33.0	41.0
54-55	37.50269569981404	40.0	37.5	41.0	33.0	41.0
56-57	37.35984183571662	40.0	37.0	41.0	33.0	41.0
58-59	37.19392311200432	40.0	37.0	41.0	33.0	41.0
60-61	37.015822368678634	40.0	36.5	41.0	32.0	41.0
62-63	36.8007455668179	39.5	36.0	41.0	32.0	41.0
64-65	36.53997255562387	39.0	35.5	41.0	31.5	41.0
66-67	36.26961399393167	39.0	35.0	41.0	31.0	41.0
68-69	36.009372373255395	38.5	35.0	41.0	31.0	41.0
70-71	35.751579523212015	37.5	35.0	40.0	31.0	41.0
72-73	35.49190358502289	37.0	35.0	40.0	31.0	41.0
74-75	35.18433796652942	37.0	35.0	39.0	31.0	41.0
76-77	34.84884153896958	36.0	35.0	39.0	31.0	41.0
78-79	34.513324590231214	36.0	35.0	38.5	31.0	40.0
80-81	34.18241140588704	35.0	35.0	37.0	30.5	39.0
82-83	33.84073891363581	35.0	35.0	37.0	30.0	39.0
84-85	33.549389076414656	35.0	34.5	36.5	30.0	38.5
86-87	33.304076343104384	35.0	34.0	36.0	30.0	37.0
88-89	33.06080519704247	35.0	34.0	36.0	30.0	37.0
90-91	32.849129375500034	35.0	34.0	35.5	29.0	37.0
92-93	32.69008443654919	35.0	34.0	35.0	29.0	36.0
94-95	32.552011467018545	35.0	34.0	35.0	29.0	36.0
96-97	32.41388152421594	35.0	34.0	35.0	29.0	36.0
98-99	32.289615662627504	35.0	34.0	35.0	29.0	36.0
100	32.185267089888974	35.0	34.0	35.0	29.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.21816785361513524
1101	2	0.20836814391904568
1101	3	0.24457468068566612
1101	4	0.1325583683458902
1101	5	0.16443012257497003
1101	6	0.1784503903720278
1101	7	0.19481439299539005
1101	8	0.22455457685845204
1101	9	0.29274714249180533
1101	10-11	0.25668485726412626
1101	12-13	0.23540803179173508
1101	14-15	0.3675619085716306
1101	16-17	0.3591832238181638
1101	18-19	0.3572149432060101
1101	20-21	0.35293603053227685
1101	22-23	0.38460336089157465
1101	24-25	0.364936152111369
1101	26-27	0.39808611891213275
1101	28-29	0.36152899778110736
1101	30-31	0.4112265378563862
1101	32-33	0.41787179939409924
1101	34-35	0.4206575484374042
1101	36-37	0.44295479412556205
1101	38-39	0.43378854848688775
1101	40-41	0.4286047974297489
1101	42-43	0.4610247420109914
1101	44-45	0.45655560507550064
1101	46-47	0.3735169326448897
1101	48-49	0.34681669313693675
1101	50-51	0.4081387346487375
1101	52-53	0.32527144124843943
1101	54-55	0.35996270257253116
1101	56-57	0.3813433390954941
1101	58-59	0.40513427820908987
1101	60-61	0.3851004136904166
1101	62-63	0.4104429546734565
1101	64-65	0.4499096777315472
1101	66-67	0.4322811163100013
1101	68-69	0.4253169075027259
1101	70-71	0.37012763938007254
1101	72-73	0.39175683171195175
1101	74-75	0.3836380798311083
1101	76-77	0.36138943713465466
1101	78-79	0.3628727462827612
1101	80-81	0.3568618358863205
1101	82-83	0.38015059707058185
1101	84-85	0.3341857987744987
1101	86-87	0.32045479930087595
1101	88-89	0.355798357303172
1101	90-91	0.39956187876242666
1101	92-93	0.4161126790257583
1101	94-95	0.3671258185681481
1101	96-97	0.38149513667511314
1101	98-99	0.40636706729293337
1101	100	0.4317111365569559
1102	1	0.0796371896750685
1102	2	0.12136574457463922
1102	3	0.13063513950321948
1102	4	0.08980503232342585
1102	5	0.08748098438368856
1102	6	0.06523317495241088
1102	7	0.12458627346967432
1102	8	0.17464849358997014
1102	9	0.2026169881441362
1102	10-11	0.16474127324056553
1102	12-13	0.1573657429686719
1102	14-15	0.29026930885014934
1102	16-17	0.28749426340598916
1102	18-19	0.2623570160947182
1102	20-21	0.24635914511895862
1102	22-23	0.23303605744092692
1102	24-25	0.21988276151711972
1102	26-27	0.2006657331362547
1102	28-29	0.2009220199232118
1102	30-31	0.15005831284388904
1102	32-33	0.20829132289290442
1102	34-35	0.23163425958093598
1102	36-37	0.1838072152315604
1102	38-39	0.13944207241657125
1102	40-41	0.12828240050748718
1102	42-43	0.15177907550459935
1102	44-45	0.24511930785003955
1102	46-47	0.22172437950008828
1102	48-49	0.23512325059512307
1102	50-51	0.2518422939553062
1102	52-53	0.2410103542836879
1102	54-55	0.25830402695959975
1102	56-57	0.2624870816641547
1102	58-59	0.2549706919094348
1102	60-61	0.21466418896675066
1102	62-63	0.13215508910459306
1102	64-65	0.21824235869632957
1102	66-67	0.27003287285565136
1102	68-69	0.2815927408731298
1102	70-71	0.252486856292947
1102	72-73	0.22368985909217542
1102	74-75	0.1658455592862893
1102	76-77	0.21141090371077098
1102	78-79	0.12168079113385488
1102	80-81	0.10393689042676613
1102	82-83	0.14723914962888074
1102	84-85	0.15982973889761354
1102	86-87	0.13266798670426994
1102	88-89	0.14484798060735216
1102	90-91	0.15984009458718162
1102	92-93	0.1655924068114345
1102	94-95	0.17802875564272114
1102	96-97	0.15789067248284283
1102	98-99	0.1861597874204861
1102	100	0.19897192695032118
1103	1	0.16369299814472527
1103	2	0.1851826476634102
1103	3	0.15016326177862283
1103	4	0.10188994942863161
1103	5	0.10970522577021313
1103	6	0.150408793144841
1103	7	0.12938353073800357
1103	8	0.12777392657187647
1103	9	0.241321888383645
1103	10-11	0.21660018217428245
1103	12-13	0.2226043945765852
1103	14-15	0.28850671697047403
1103	16-17	0.25823394746154804
1103	18-19	0.2165566088328461
1103	20-21	0.2651692648852162
1103	22-23	0.28974028007741737
1103	24-25	0.2444062006601655
1103	26-27	0.2878433867629795
1103	28-29	0.27201571728171814
1103	30-31	0.3276835724142657
1103	32-33	0.2648618042535702
1103	34-35	0.2612415809021442
1103	36-37	0.21696684776829045
1103	38-39	0.2633753508338259
1103	40-41	0.31193659136401664
1103	42-43	0.2619397371345116
1103	44-45	0.2718024425355239
1103	46-47	0.31423345334248154
1103	48-49	0.2688010029599468
1103	50-51	0.3028355307596229
1103	52-53	0.24675320758453267
1103	54-55	0.27074087176225703
1103	56-57	0.25850036653365294
1103	58-59	0.26643995431390977
1103	60-61	0.19372977790527557
1103	62-63	0.24045172258728797
1103	64-65	0.30221773279999553
1103	66-67	0.29738037498574244
1103	68-69	0.3113535292145073
1103	70-71	0.2555225426073804
1103	72-73	0.21836212150160605
1103	74-75	0.191688800218067
1103	76-77	0.1983737180939258
1103	78-79	0.16971505690300148
1103	80-81	0.18221331103767824
1103	82-83	0.15125237236775035
1103	84-85	0.18211964768811129
1103	86-87	0.11372915736776434
1103	88-89	0.13965179659452787
1103	90-91	0.15541758999957267
1103	92-93	0.1965853796335182
1103	94-95	0.18181821332986914
1103	96-97	0.18820348997252978
1103	98-99	0.23155477776689537
1103	100	0.25429968641787326
1104	1	0.09963968855385019
1104	2	0.12851483161483657
1104	3	0.13206907422008385
1104	4	0.026619158482333205
1104	5	0.09321130948867307
1104	6	0.12372011667240201
1104	7	0.07137162405638264
1104	8	0.11751912404255194
1104	9	0.1556250312855525
1104	10-11	0.1831087802371485
1104	12-13	0.19380930421000642
1104	14-15	0.2426315827329688
1104	16-17	0.22725853394138085
1104	18-19	0.20892029556966207
1104	20-21	0.1838179720307096
1104	22-23	0.19864947661943688
1104	24-25	0.20677130553983858
1104	26-27	0.22510035825030172
1104	28-29	0.226729069439763
1104	30-31	0.23536147214471015
1104	32-33	0.2263266314517125
1104	34-35	0.2404427004344356
1104	36-37	0.263476214944923
1104	38-39	0.26213912197301426
1104	40-41	0.25172248896266325
1104	42-43	0.22209013747394124
1104	44-45	0.19806587629395978
1104	46-47	0.23457971464902272
1104	48-49	0.2734875933900014
1104	50-51	0.28285022397758297
1104	52-53	0.2835437215812604
1104	54-55	0.18336733236362335
1104	56-57	0.22976110124601945
1104	58-59	0.241923666828896
1104	60-61	0.24860976184824324
1104	62-63	0.2654434882094492
1104	64-65	0.2633504686834627
1104	66-67	0.2903564220955346
1104	68-69	0.3288806025908144
1104	70-71	0.2812270965614232
1104	72-73	0.24808843994558316
1104	74-75	0.262086869485195
1104	76-77	0.2512277019888529
1104	78-79	0.2325837454525299
1104	80-81	0.21328036060291566
1104	82-83	0.27291387539331424
1104	84-85	0.2381664544078177
1104	86-87	0.2244954777330932
1104	88-89	0.24507592137737078
1104	90-91	0.23070467815202278
1104	92-93	0.2758386931128456
1104	94-95	0.29389409998365323
1104	96-97	0.29004901990620624
1104	98-99	0.29519014979494784
1104	100	0.2675976347997633
1105	1	-0.018056014702423795
1105	2	0.06599639262336865
1105	3	0.11062515923896399
1105	4	-0.08317501252806636
1105	5	-0.1090872182526681
1105	6	-0.12254993360879496
1105	7	-0.3850487496963808
1105	8	-0.383982832043678
1105	9	-0.3072043819290329
1105	10-11	-0.2889161634381452
1105	12-13	-0.17165094674231796
1105	14-15	-0.18111719173327856
1105	16-17	-0.13305499898527273
1105	18-19	-0.1355471726314761
1105	20-21	-0.09855387174129504
1105	22-23	-0.05632616151775949
1105	24-25	-0.060008303110002714
1105	26-27	-0.12018474648316868
1105	28-29	-0.15828837771094584
1105	30-31	-0.12001799615929798
1105	32-33	-0.14957339360074684
1105	34-35	-0.15047845667747595
1105	36-37	-0.09592327857337324
1105	38-39	-0.10373411904472363
1105	40-41	-0.1221845538146411
1105	42-43	-0.07142951238675721
1105	44-45	-0.09915267859766885
1105	46-47	-0.12858241671201398
1105	48-49	-0.16331401852099248
1105	50-51	-0.07719777663616156
1105	52-53	-0.044996846511608624
1105	54-55	-0.032173804538722095
1105	56-57	-0.04366458091359249
1105	58-59	-0.08848276105047859
1105	60-61	-0.04486168657444978
1105	62-63	-0.07166216298566042
1105	64-65	-0.02999423650505406
1105	66-67	0.016204341194843153
1105	68-69	-0.039851657329542434
1105	70-71	-0.051978084878250286
1105	72-73	-0.026973833639495126
1105	74-75	-0.11555769004070982
1105	76-77	-0.1402340011108052
1105	78-79	-0.14854184643729695
1105	80-81	-0.1672390200954723
1105	82-83	-0.16652008766136817
1105	84-85	-0.19685872953421324
1105	86-87	-0.16727499268981916
1105	88-89	-0.15400173534719386
1105	90-91	-0.13939871311983154
1105	92-93	-0.17541128629905245
1105	94-95	-0.14161465147594043
1105	96-97	-0.1845636854221695
1105	98-99	-0.15169246148121118
1105	100	-0.1543207311436774
1106	1	0.0705016336083375
1106	2	0.04945340222003125
1106	3	0.07134362014784301
1106	4	0.07653486644501584
1106	5	0.08704195011602422
1106	6	0.0718452282296127
1106	7	0.07384701463438859
1106	8	0.07004008107170279
1106	9	0.04746869550444899
1106	10-11	0.1243668329967278
1106	12-13	0.12163478784736981
1106	14-15	0.1237310099874378
1106	16-17	0.13829043124349027
1106	18-19	0.12649431058339644
1106	20-21	0.1260125156152796
1106	22-23	0.11650867510594765
1106	24-25	0.16215191121586514
1106	26-27	0.13905097546144418
1106	28-29	0.1650463857133957
1106	30-31	0.1803244528432444
1106	32-33	0.10367808049631577
1106	34-35	0.11529983840524238
1106	36-37	0.11654377281116268
1106	38-39	0.09471519317776966
1106	40-41	0.13680269024136038
1106	42-43	0.14368964768374326
1106	44-45	0.08536675404770477
1106	46-47	0.1121981146886526
1106	48-49	0.019362040714945294
1106	50-51	0.013221905076903795
1106	52-53	-0.005495404234821422
1106	54-55	0.020543590394758837
1106	56-57	0.012974581838797405
1106	58-59	-0.03388133392202519
1106	60-61	-0.0025274336644685036
1106	62-63	0.018171381403881526
1106	64-65	-0.04706272349215368
1106	66-67	-0.07639827156345547
1106	68-69	-0.04330795916843755
1106	70-71	-0.03401293523680238
1106	72-73	-0.06278695291271674
1106	74-75	-0.09713559937510752
1106	76-77	-0.09273806537927243
1106	78-79	-0.14609031996344157
1106	80-81	-0.11783440945360013
1106	82-83	-0.04906470326365309
1106	84-85	-0.05284059954615827
1106	86-87	-0.08349918364015707
1106	88-89	-0.0864951520644226
1106	90-91	-0.03385201414036487
1106	92-93	-0.031465542168874094
1106	94-95	-0.03869204577080865
1106	96-97	-0.006401632688721293
1106	98-99	-1.5569327875653016E-4
1106	100	0.00904399809372336
1107	1	-0.025754692358631814
1107	2	0.016377597401707078
1107	3	0.016605204683870056
1107	4	-0.055639907106446174
1107	5	-0.0781338785469714
1107	6	-0.11201790578532211
1107	7	-0.03551323223380365
1107	8	-0.04551522976755251
1107	9	-0.041620638282722666
1107	10-11	0.020189055502086717
1107	12-13	0.008114146431779545
1107	14-15	0.05172374373347566
1107	16-17	0.04727459364936237
1107	18-19	-0.0018550314432275172
1107	20-21	0.0225299498315934
1107	22-23	0.03511096176743678
1107	24-25	0.023599021854529667
1107	26-27	0.02093304505754645
1107	28-29	-0.011842030326974395
1107	30-31	0.04731250684505284
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2216	26-27	-0.5700847093440444
2216	28-29	-0.47948981619397557
2216	30-31	-0.5658181059954686
2216	32-33	-0.5458686757430229
2216	34-35	-0.5080874749063966
2216	36-37	-0.5505056398734425
2216	38-39	-0.608966597719899
2216	40-41	-0.6001574379343495
2216	42-43	-0.5525379118769678
2216	44-45	-0.5945232427887959
2216	46-47	-0.5374369068246239
2216	48-49	-0.6102529482128176
2216	50-51	-0.45426501283316156
2216	52-53	-0.5111908039324362
2216	54-55	-0.5252276779638052
2216	56-57	-0.5349315929644973
2216	58-59	-0.5802525771905067
2216	60-61	-0.6071986009127102
2216	62-63	-0.5889973062454388
2216	64-65	-0.5244323914860374
2216	66-67	-0.5347962347356869
2216	68-69	-0.6367638707035752
2216	70-71	-0.5871007079462984
2216	72-73	-0.591247648378328
2216	74-75	-0.4949278069725054
2216	76-77	-0.45602316939848464
2216	78-79	-0.43940273332156465
2216	80-81	-0.4877107899110271
2216	82-83	-0.5049467113823312
2216	84-85	-0.4908351130462023
2216	86-87	-0.41893300154037405
2216	88-89	-0.4434528675507039
2216	90-91	-0.3961177647745444
2216	92-93	-0.4800109928242833
2216	94-95	-0.5238913883129399
2216	96-97	-0.5255323413222897
2216	98-99	-0.5183613534197136
2216	100	-0.536884852990049
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	42.0
3	278.0
4	138.0
5	215.0
6	265.0
7	570.0
8	1116.0
9	1790.0
10	2618.0
11	3909.0
12	6313.0
13	9419.0
14	9991.0
15	12068.0
16	14551.0
17	21197.0
18	25103.0
19	13872.0
20	8488.0
21	7557.0
22	7613.0
23	7929.0
24	9327.0
25	11132.0
26	13996.0
27	17849.0
28	22322.0
29	28356.0
30	35961.0
31	44844.0
32	57204.0
33	74100.0
34	103105.0
35	162785.0
36	274315.0
37	624943.0
38	1264564.0
39	797491.0
40	6155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.961758948489226	21.77228302153265	14.781834035570093	24.48412399440803
2	13.270671717758114	22.363095350601732	40.723965772825096	23.642267158815063
3	15.051851259815773	28.250660595256676	32.11073482818043	24.586753316747124
4	10.523824477905015	19.097439458961595	40.45285990132843	29.92587616180496
5	11.32493639109694	38.297919449513984	36.41523632702226	13.961907832366812
6	27.53674843546265	40.51701489216526	18.807147094457637	13.13908957791446
7	23.727153650434143	33.92917655260942	24.93803819153334	17.4056316054231
8	23.43991115409758	35.74781199684298	22.422438720655727	18.389838128403717
9	24.39738614188613	16.122517916203925	23.09529036252552	36.38480557938442
10-11	21.988874821080977	27.385377742243737	30.90551590377835	19.720231532896936
12-13	23.580562231688965	25.517302458680202	30.489084488122153	20.41305082150868
14-15	21.11028486365972	27.116023773245296	28.492022796869225	23.281668566225758
16-17	19.61357540763566	30.253009930360303	28.81289572460146	21.320518937402575
18-19	19.43412310168973	28.4196451402204	32.13026574116151	20.015966016928353
20-21	20.795392779407322	27.905103590099177	31.597876166028215	19.701627464465286
22-23	20.402428737449668	28.05626749701903	31.554645242538925	19.98665852299238
24-25	20.251724928425563	27.92402133448828	31.630069175347447	20.194184561738705
26-27	20.109934722039775	27.675649097245408	31.795203985018915	20.419212195695902
28-29	20.138759598974527	27.749030601285746	31.80578246451186	20.30642733522787
30-31	20.517531325031836	28.228832854317677	31.31070726904911	19.94292855160138
32-33	19.915786284508265	27.94976178106324	31.787943561932064	20.346508372496434
34-35	20.142878591001935	27.6150033321776	31.672132086007988	20.569985990812476
36-37	20.745534021794427	28.13601373033798	30.890263146809417	20.228189101058184
38-39	20.5303156211779	28.14864099128917	31.08013909997612	20.24090428755681
40-41	20.307389338676966	27.92484586248825	31.221794111222035	20.54597068761275
42-43	20.255411678554612	28.020449459318176	31.046083242967683	20.678055619159537
44-45	20.596418792476197	28.169543089503996	30.73698555530939	20.49705256271042
46-47	20.64894373523046	28.592818433764073	30.12527959433557	20.632958236669896
48-49	20.634885917839675	28.344290140520663	30.16447969308249	20.856344248557175
50-51	20.638368938833857	28.240380466451047	30.031397716834068	21.089852877881025
52-53	20.67033275919005	28.41731135153905	29.80124957843806	21.111106310832838
54-55	20.851304087435814	28.883093724778732	29.567627234019113	20.697974953766344
56-57	20.61900459623995	28.44246815547183	29.728319070102515	21.21020817818571
58-59	20.415081495966415	28.053845783091543	30.142146592158074	21.38892612878397
60-61	20.913263277554677	28.522449508603735	29.57239513403281	20.991892079808775
62-63	20.680541683508885	28.471380111359796	29.70373088526474	21.14434731986658
64-65	20.61743905952519	28.2021746508902	30.021215118384248	21.15917117120036
66-67	20.674042105996467	28.31683481109437	29.652436111822905	21.35668697108625
68-69	21.122880869672052	28.420155179001288	29.502641445241146	20.954322506085518
70-71	21.13248254143982	28.155113695814332	29.635113560803717	21.077290201942127
72-73	20.830349659564174	28.341552612024124	29.797409901152644	21.030687827259058
74-75	20.857713411386484	28.334039663105674	29.62911408965208	21.179132835855757
76-77	20.92397665871649	28.41746724230528	29.393926956827915	21.26462914215032
78-79	21.040508455766503	28.532160156653973	29.43741091074833	20.98992047683119
80-81	20.91202391928295	28.279427145810175	29.55418257354517	21.254366361361708
82-83	20.740887151224463	28.289115642125555	29.707257188150958	21.262740018499024
84-85	20.764683232517207	28.30661314528485	29.537265603187524	21.391438019010423
86-87	20.868526593508427	28.44056898932058	29.41638119184691	21.274523225324078
88-89	20.57610307405194	28.317754776005895	29.52764521689259	21.57849693304958
90-91	20.465259766998837	28.247360881780736	29.516905342771473	21.770474008448957
92-93	20.62595090665308	28.414106759688163	29.40744432069841	21.552498012960346
94-95	20.43963061469711	28.178900754701697	29.620394497023046	21.761074133578152
96-97	20.413913067427984	28.251107998964738	29.60263249240126	21.73234644120602
98-99	20.371948995129085	28.187541161377982	29.544911434350656	21.895598409142274
100	20.49955945209504	28.16992646913883	29.417826002074342	21.91268807669179
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1135.0
1	764.0
2	1646.0
3	2467.5
4	1284.0
5	683.0
6	1468.5
7	3804.0
8	6406.0
9	6703.0
10	4408.0
11	2470.0
12	2307.5
13	2609.0
14	2970.0
15	3338.5
16	3810.5
17	4301.0
18	4871.0
19	5764.5
20	7115.0
21	8767.0
22	11286.5
23	15214.5
24	20597.0
25	27357.5
26	35838.5
27	46380.5
28	58643.5
29	72266.0
30	84662.0
31	95513.0
32	105382.5
33	113196.5
34	120110.0
35	125796.0
36	129863.0
37	133435.0
38	135943.5
39	136218.5
40	135639.0
41	133999.5
42	131119.0
43	128267.5
44	123956.5
45	118900.5
46	114881.0
47	111366.5
48	107546.0
49	104131.5
50	100529.0
51	94744.5
52	87261.5
53	80540.0
54	75257.0
55	70469.5
56	64976.0
57	58877.5
58	53946.0
59	49063.0
60	42569.0
61	35460.5
62	29466.5
63	24082.0
64	18874.0
65	14804.0
66	11564.0
67	8870.5
68	6733.0
69	5027.5
70	3715.5
71	2735.5
72	2076.0
73	1615.0
74	1234.5
75	902.5
76	649.5
77	486.0
78	353.5
79	230.0
80	140.0
81	62.5
82	30.0
83	16.5
84	9.0
85	11.5
86	9.0
87	4.0
88	3.0
89	3.5
90	4.5
91	2.5
92	2.0
93	2.0
94	4.0
95	7.0
96	9.5
97	12.0
98	7.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008748502426494354
2	2.7001550699056646E-5
3	3.5102015908773643E-4
4	0.002538145765711325
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.100465209716995E-5
24-25	1.0800620279622658E-4
26-27	5.535317893306613E-4
28-29	9.58555049816511E-4
30-31	0.0017010976940405687
32-33	0.004725271372334913
34-35	0.005589320994704726
36-37	0.004293246561150007
38-39	0.003010672902944816
40-41	0.003834220199266044
42-43	5.130294632820762E-4
44-45	5.265302386316046E-4
46-47	0.003631708569023119
48-49	0.0023086325847693433
50-51	9.99057375865096E-4
52-53	5.400310139811329E-5
54-55	5.400310139811329E-4
56-57	7.830449702726427E-4
58-59	6.885395428259445E-4
60-61	3.645209344372647E-4
62-63	0.0
64-65	0.0
66-67	7.560434195735861E-4
68-69	7.020403181754729E-4
70-71	0.0021196217298759465
72-73	8.100465209716995E-5
74-75	0.00201161552707972
76-77	2.7001550699056647E-4
78-79	0.0011340651293603793
80-81	9.58555049816511E-4
82-83	0.0014175814117004738
84-85	0.0013230759842537756
86-87	3.240186083886798E-4
88-89	0.0015255876144967006
90-91	0.002052117853128305
92-93	0.0031726822071391557
94-95	0.0011070635786613226
96-97	0.0021736248312740603
98-99	0.0021601240559245317
100	0.003996229503460384
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3703491.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.03101863339386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.16669346978883	56.141552041603546
2	13.433072267932836	18.81463468598661
3	3.363637961402171	7.0667697825283895
4	1.2212104395815122	3.420904441985119
5	0.5677713685890304	1.9880803646582956
6	0.3219172114677493	1.3526514140824886
7	0.21149717807203805	1.036795397293118
8	0.13264929568279354	0.7431652238134628
9	0.10623402385306448	0.6695709213558929
>10	0.44562881155320855	5.353509537207904
>50	0.020754211845407478	0.986677335299504
>100	0.00804577265549114	0.9252596301350247
>500	5.405141892859546E-4	0.27078241292394106
>1k	2.3164892442217156E-4	0.3360784067878344
>5k	3.860815407036169E-5	0.21657184540390548
>10k+	7.721630814072337E-5	0.67699655893488
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14093	0.3805328540018053	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10946	0.2955589739518741	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8010	0.21628242109944373	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.400310139811329E-5	0.0
10-11	0.0	0.0	0.0	6.750387674764162E-5	0.0
12-13	0.0	0.0	0.0	1.0800620279622658E-4	0.0
14-15	0.0	2.7001550699056646E-5	0.0	1.4850852884481155E-4	0.0
16-17	0.0	5.400310139811329E-5	0.0	1.8901085489339653E-4	0.0
18-19	0.0	5.400310139811329E-5	0.0	1.8901085489339653E-4	0.0
20-21	0.0	5.400310139811329E-5	0.0	2.0251163024292485E-4	0.0
22-23	0.0	5.400310139811329E-5	0.0	4.1852403583537805E-4	0.0
24-25	0.0	5.400310139811329E-5	0.0	6.075348907287746E-4	0.0
26-27	0.0	5.400310139811329E-5	0.0	9.990573758650958E-4	0.0
28-29	0.0	5.400310139811329E-5	0.0	0.002241128708021702	0.0
30-31	0.0	5.400310139811329E-5	0.0	0.00515729618351982	0.0
32-33	0.0	5.400310139811329E-5	0.0	0.012636725727158512	0.0
34-35	0.0	5.400310139811329E-5	0.0	0.02528695222966655	0.0
36-37	0.0	5.400310139811329E-5	0.0	0.04390452143666611	0.0
38-39	0.0	5.400310139811329E-5	0.0	0.06812491241371992	0.0
40-41	0.0	5.400310139811329E-5	0.0	0.09389789255596949	0.0
42-43	0.0	5.400310139811329E-5	0.0	0.11856380911955773	0.0
44-45	0.0	5.400310139811329E-5	0.0	0.15165420950125164	0.0
46-47	0.0	5.400310139811329E-5	0.0	0.19026642700090268	0.0
48-49	0.0	5.400310139811329E-5	0.0	0.23033672823830273	0.0
50-51	0.0	6.750387674764162E-5	0.0	0.27085255506223727	0.0
52-53	0.0	8.100465209716993E-5	0.0	0.3133124935365038	0.0
54-55	0.0	8.100465209716993E-5	0.0	0.36138875455617414	0.0
56-57	2.7001550699056646E-5	8.100465209716993E-5	0.0	0.4185510373860771	0.0
58-59	5.400310139811329E-5	8.100465209716993E-5	0.0	0.4825987156442394	0.0
60-61	5.400310139811329E-5	8.100465209716993E-5	0.0	0.5515066730282321	0.0
62-63	5.400310139811329E-5	8.100465209716993E-5	0.0	0.6199016009489424	0.0
64-65	5.400310139811329E-5	8.100465209716993E-5	0.0	0.6971125351728951	0.0
66-67	5.400310139811329E-5	1.3500775349528323E-4	0.0	0.7868926912472582	0.0
68-69	6.750387674764162E-5	1.3500775349528323E-4	0.0	0.8896065901064698	0.0
70-71	8.100465209716993E-5	1.3500775349528323E-4	0.0	1.0049437139174904	0.0
72-73	8.100465209716993E-5	1.3500775349528323E-4	0.0	1.1270447261786245	0.0
74-75	8.100465209716993E-5	1.3500775349528323E-4	0.0	1.253479487326957	0.0
76-77	8.100465209716993E-5	1.3500775349528323E-4	0.0	1.3929694982382839	0.0
78-79	8.100465209716993E-5	1.3500775349528323E-4	0.0	1.5542497605637493	0.0
80-81	8.100465209716993E-5	1.3500775349528323E-4	0.0	1.7337560696110779	0.0
82-83	8.100465209716993E-5	1.3500775349528323E-4	0.0	1.9275461989782072	0.0
84-85	8.100465209716993E-5	1.3500775349528323E-4	0.0	2.126426120652109	0.0
86-87	8.100465209716993E-5	1.3500775349528323E-4	0.0	2.3405484176956284	0.0
88	8.100465209716993E-5	1.3500775349528323E-4	0.0	2.5188126554108003	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6105	0.0	59.515266	1
GTATCAA	12320	0.0	43.875458	1
ATCAACG	15480	0.0	34.551334	3
TCAACGC	15560	0.0	34.4341	4
TATCAAC	16150	0.0	33.467155	2
CAACGCA	16130	0.0	33.187687	5
AACGCAG	16670	0.0	32.140812	6
ACGCAGA	18735	0.0	28.44769	7
CGCAGAG	18945	0.0	28.18197	8
GTGGTAT	2470	0.0	25.690428	1
GCAGAGT	20785	0.0	25.528868	9
TGGTATC	2515	0.0	24.293991	2
GTACATG	9485	0.0	23.836489	1
TACATGG	9470	0.0	23.07791	2
AGAGTAC	19705	0.0	22.551357	10-11
ACATGGG	9355	0.0	22.457281	3
GAGTACT	15800	0.0	21.551075	12-13
CAGAGTA	20680	0.0	21.124502	10-11
AGTACTT	16615	0.0	20.45152	12-13
TCTGTCG	965	0.0	18.507336	8
>>END_MODULE