FastQCFastQC Report
Wed 25 May 2016
SRR2431357_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431357_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847762
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA203581.101765270635504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC3150.041.777613
TATAACG3250.040.4921462
TACCTGG17600.039.5225682
GTACCTG18750.037.8505131
TCTGTCG7200.035.9026348
TAACGCA3900.033.7434544
GTCTCGC7300.033.4793171
GGTATCA29100.032.948321
TCGCTCT8150.031.7176634
GTATAAC5700.031.3332041
GTATCAA43550.030.8655471
CTGTCGC8350.030.3950869
CTCGCTC8600.028.418493
TCTCGCT8950.027.8322892
ACCTGGG24450.027.2964153
CAACGCA47850.026.3238245
TCAACGC48450.025.9978314
ATCAACG48700.025.960883
CGCTCTG8750.025.7827535
GTACACG1659.3245035E-825.6362611
Produced by FastQC (version 0.11.5)