##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431357_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1847762
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.779820128349865	33.0	31.0	34.0	26.0	34.0
2	30.887349669492068	34.0	31.0	34.0	26.0	34.0
3	30.90731977386698	34.0	31.0	34.0	26.0	34.0
4	34.04828056860137	37.0	35.0	37.0	30.0	37.0
5	34.20498906244419	37.0	35.0	37.0	30.0	37.0
6	34.18014170656178	37.0	35.0	37.0	30.0	37.0
7	34.13995200680607	37.0	35.0	37.0	30.0	37.0
8	34.08525719221415	37.0	35.0	37.0	30.0	37.0
9	35.51063773364751	39.0	37.0	39.0	28.0	39.0
10-11	35.54679390527568	39.0	37.0	39.0	29.5	39.0
12-13	35.5003165992157	39.0	37.0	39.0	29.0	39.0
14-15	36.866414343405694	41.0	38.0	41.0	29.5	41.0
16-17	36.87130728957517	41.0	38.0	41.0	29.5	41.0
18-19	36.883054473465734	41.0	38.0	41.0	29.5	41.0
20-21	36.93607970074068	41.0	38.0	41.0	29.0	41.0
22-23	36.951407161744854	40.5	38.0	41.0	29.0	41.0
24-25	36.99029420455665	41.0	38.0	41.0	29.0	41.0
26-27	37.00179460341754	40.5	38.0	41.0	30.0	41.0
28-29	36.98040196735294	40.0	38.0	41.0	30.0	41.0
30-31	36.9205170904045	40.0	38.0	41.0	30.0	41.0
32-33	36.82787664212166	40.0	38.0	41.0	30.0	41.0
34-35	36.76638928606606	40.0	38.0	41.0	29.5	41.0
36-37	36.66017728473689	40.0	37.0	41.0	29.0	41.0
38-39	36.544804200973935	40.0	37.0	41.0	28.5	41.0
40-41	36.45820078559901	40.0	37.0	41.0	28.0	41.0
42-43	36.33468812541875	40.0	37.0	41.0	28.0	41.0
44-45	36.20020083755375	40.0	36.5	41.0	27.5	41.0
46-47	36.0828199194485	40.0	36.0	41.0	27.0	41.0
48-49	35.96401674025118	40.0	36.0	41.0	27.0	41.0
50-51	35.83594234538864	40.0	35.5	41.0	27.0	41.0
52-53	35.67476195527346	40.0	35.0	41.0	27.0	41.0
54-55	35.51364732038	40.0	35.0	41.0	27.0	41.0
56-57	35.346917784866235	39.0	35.0	41.0	27.0	41.0
58-59	35.13614821605813	39.0	35.0	41.0	26.5	41.0
60-61	34.96230764568164	39.0	35.0	41.0	26.0	41.0
62-63	34.776960993894235	39.0	34.0	41.0	26.0	41.0
64-65	34.510817681064985	38.0	34.0	40.0	26.0	41.0
66-67	34.2144218248887	37.5	34.0	40.0	25.5	41.0
68-69	33.87854821129561	37.0	34.0	40.0	25.0	41.0
70-71	33.59910069586884	36.5	33.0	39.5	24.5	41.0
72-73	33.33678633936621	36.0	33.0	39.0	24.0	41.0
74-75	33.00182301616766	36.0	33.0	39.0	23.5	41.0
76-77	32.65100456660544	35.0	33.0	38.5	22.5	40.0
78-79	32.311957654719606	35.0	33.0	37.0	21.5	39.5
80-81	31.96417368687093	35.0	32.5	37.0	20.0	39.0
82-83	31.641538791251254	35.0	32.0	36.5	19.5	39.0
84-85	31.340086818540485	35.0	32.0	36.0	18.0	38.5
86-87	31.065410750951692	35.0	32.0	36.0	17.5	37.0
88-89	30.80112184361406	35.0	32.0	35.5	11.5	37.0
90-91	30.576683847811566	35.0	31.5	35.0	7.0	36.5
92-93	30.338737077610645	35.0	31.0	35.0	2.0	36.0
94-95	30.101121789494535	35.0	31.0	35.0	2.0	36.0
96-97	29.888965407882615	35.0	31.0	35.0	2.0	36.0
98-99	29.74156438978613	35.0	31.0	35.0	2.0	36.0
100	29.675215747482632	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2268765060622755
1101	2	0.19988665893394497
1101	3	0.2641556776956939
1101	4	0.10426656378012211
1101	5	0.006023826164735624
1101	6	0.09862096185554492
1101	7	0.0986258285086663
1101	8	0.04408772102464553
1101	9	0.15649153598241128
1101	10-11	0.03898717789686401
1101	12-13	0.06821069621287279
1101	14-15	0.09123320636816601
1101	16-17	0.0642991227541927
1101	18-19	0.13337112473628565
1101	20-21	0.16623299983194073
1101	22-23	0.17829656645760394
1101	24-25	0.24462275786095944
1101	26-27	0.23862616726360386
1101	28-29	0.3007119342926572
1101	30-31	0.3154644311264718
1101	32-33	0.28737704741890724
1101	34-35	0.32752023882790837
1101	36-37	0.2714071029229075
1101	38-39	0.26464050738032086
1101	40-41	0.3291726754877544
1101	42-43	0.323129661529201
1101	44-45	0.30246495427268627
1101	46-47	0.2844666027916247
1101	48-49	0.32152489087793157
1101	50-51	0.23539909915393764
1101	52-53	0.2577052109922775
1101	54-55	0.25974186035887925
1101	56-57	0.23764289829961882
1101	58-59	0.29665406218585844
1101	60-61	0.27179768273285276
1101	62-63	0.21821024195143934
1101	64-65	0.27995343863693734
1101	66-67	0.22643408965419098
1101	68-69	0.29646396222454996
1101	70-71	0.3453358872361818
1101	72-73	0.2584418697377515
1101	74-75	0.27995787847910236
1101	76-77	0.1996230864439994
1101	78-79	0.2085467935797496
1101	80-81	0.18676822744859578
1101	82-83	0.2100086792307856
1101	84-85	0.15605773133533418
1101	86-87	0.19028438975989914
1101	88-89	0.19835835495664966
1101	90-91	0.23189374386075556
1101	92-93	0.17841122156680456
1101	94-95	0.25161438171839023
1101	96-97	0.2969129053856676
1101	98-99	0.2959608593565406
1101	100	0.3792063895872744
1102	1	0.23640941954255723
1102	2	0.2821216266952291
1102	3	0.27836020611075085
1102	4	0.2511251632703022
1102	5	0.18524035038126385
1102	6	0.14163415723716355
1102	7	0.24673199135167323
1102	8	0.3166825628300174
1102	9	0.30006253613235856
1102	10-11	0.42462183076835913
1102	12-13	0.33955085215829683
1102	14-15	0.5061661048536976
1102	16-17	0.5045757759255736
1102	18-19	0.49628610449337174
1102	20-21	0.46006415892626507
1102	22-23	0.4329779279810708
1102	24-25	0.5113670223683826
1102	26-27	0.43794203171104584
1102	28-29	0.4656628264803899
1102	30-31	0.4676307978981029
1102	32-33	0.520206931959315
1102	34-35	0.531241936233819
1102	36-37	0.5625200883779939
1102	38-39	0.526951198638379
1102	40-41	0.5708104772571332
1102	42-43	0.49019518859472555
1102	44-45	0.6051760054047932
1102	46-47	0.46640804828570026
1102	48-49	0.40338573958087665
1102	50-51	0.42723820546672187
1102	52-53	0.5205647101675694
1102	54-55	0.5498869345828936
1102	56-57	0.4997774012235965
1102	58-59	0.5054481092693806
1102	60-61	0.6087822381384598
1102	62-63	0.6257169895897619
1102	64-65	0.5975205401514003
1102	66-67	0.6162489044690034
1102	68-69	0.6336142096379547
1102	70-71	0.6485938719415358
1102	72-73	0.638457239358388
1102	74-75	0.5991219210641958
1102	76-77	0.47012653523692194
1102	78-79	0.5153870244989349
1102	80-81	0.5770452554887839
1102	82-83	0.5626878665152368
1102	84-85	0.5677172754948749
1102	86-87	0.5752004191496134
1102	88-89	0.5577784329293607
1102	90-91	0.5351449809277824
1102	92-93	0.6171126760577295
1102	94-95	0.5993171857369823
1102	96-97	0.5118461787399937
1102	98-99	0.5676279008920062
1102	100	0.4501495594778149
1103	1	0.13481727363874896
1103	2	0.19186202969567034
1103	3	0.2048449832838628
1103	4	0.23779967422779436
1103	5	0.127445821774117
1103	6	0.15845942565898952
1103	7	0.20579655967054578
1103	8	0.20718337177250845
1103	9	0.23875572630738162
1103	10-11	0.20407873619562622
1103	12-13	0.28243326133610935
1103	14-15	0.21233429135170923
1103	16-17	0.16868005596676028
1103	18-19	0.10648172149586799
1103	20-21	0.14069954648325478
1103	22-23	0.16792865602931073
1103	24-25	0.0743332674044197
1103	26-27	0.15745776931951383
1103	28-29	0.14797897591412834
1103	30-31	0.004993277674543606
1103	32-33	-0.06274166342235077
1103	34-35	0.0338717347336015
1103	36-37	0.029639150004705073
1103	38-39	0.06450819231391591
1103	40-41	0.03097998747922759
1103	42-43	-0.02187423337909422
1103	44-45	-0.059977693668606946
1103	46-47	-0.040363903471906326
1103	48-49	-0.03925729615966844
1103	50-51	-0.036995505373099036
1103	52-53	-0.015015522219549382
1103	54-55	-0.020822360901732395
1103	56-57	-0.07847307678787274
1103	58-59	-0.05575537653735552
1103	60-61	9.207879251960094E-4
1103	62-63	-0.0637796275598177
1103	64-65	-0.054723172159228284
1103	66-67	-0.08207805537803381
1103	68-69	-0.07947668608200331
1103	70-71	-0.15651018747896472
1103	72-73	-0.0958930327404488
1103	74-75	-0.12316885699139846
1103	76-77	-0.09359790953435976
1103	78-79	-0.142649819154542
1103	80-81	-0.11720867891333597
1103	82-83	-0.08637706950476698
1103	84-85	-0.05480552197533939
1103	86-87	1.0775846422106383E-4
1103	88-89	-0.0628860030171019
1103	90-91	-0.012054239545815193
1103	92-93	-0.0992867944526381
1103	94-95	-0.09062692369087344
1103	96-97	-0.08499012071580836
1103	98-99	-0.21303432884766238
1103	100	-0.3240701787189799
1104	1	0.2112726226068098
1104	2	0.20412217388297194
1104	3	0.2718656635885033
1104	4	0.24440915370891503
1104	5	0.17362369859426252
1104	6	0.09383341997367722
1104	7	0.1517793662904765
1104	8	0.14258628658660655
1104	9	0.23330779143864078
1104	10-11	0.19673879934826033
1104	12-13	0.23083407941400935
1104	14-15	0.18969827373310721
1104	16-17	0.2467053442232512
1104	18-19	0.1708891053861521
1104	20-21	0.3069172534169411
1104	22-23	0.15409678291871387
1104	24-25	0.14553968890065505
1104	26-27	0.20545455943042867
1104	28-29	0.24485234063527628
1104	30-31	0.2254719262568301
1104	32-33	0.1982388560442203
1104	34-35	0.29921302168150277
1104	36-37	0.254636912961125
1104	38-39	0.22951002132075615
1104	40-41	0.22768985115916252
1104	42-43	0.2119006863915729
1104	44-45	0.27161150924950306
1104	46-47	0.22540431876925027
1104	48-49	0.2682473586806111
1104	50-51	0.26132152200043635
1104	52-53	0.17433972960712651
1104	54-55	0.12545809778884376
1104	56-57	0.1346987789847347
1104	58-59	0.1742776314880814
1104	60-61	0.09610044221069813
1104	62-63	0.08306406501527164
1104	64-65	0.2412787119683557
1104	66-67	0.23207630993512396
1104	68-69	0.11490658068883874
1104	70-71	0.1238554420823661
1104	72-73	0.01882306316393567
1104	74-75	-0.010328155254086369
1104	76-77	0.02628779722465424
1104	78-79	0.10269738074695312
1104	80-81	0.0585214384222823
1104	82-83	0.11360159804765146
1104	84-85	0.1756744652538238
1104	86-87	0.23185638205604775
1104	88-89	0.17437240522438202
1104	90-91	0.04993985752254915
1104	92-93	0.06546391299002607
1104	94-95	0.10042855284236296
1104	96-97	0.042793101528921795
1104	98-99	0.09487979444958938
1104	100	0.06556182224312579
1105	1	0.1777705506233147
1105	2	0.259326275628851
1105	3	0.18642754375752446
1105	4	0.20063390997530917
1105	5	0.1770951311145481
1105	6	0.22483928544228604
1105	7	0.19373294989468803
1105	8	0.23082826136823797
1105	9	0.29471865882586457
1105	10-11	0.24818221897827897
1105	12-13	0.2151115998218458
1105	14-15	0.30270935910723296
1105	16-17	0.31688766185895645
1105	18-19	0.259703310340484
1105	20-21	0.23443930869860452
1105	22-23	0.15047073890846008
1105	24-25	0.12165997707722909
1105	26-27	0.20132269310573037
1105	28-29	0.2146707344602632
1105	30-31	0.2586875915717215
1105	32-33	0.33489121185387916
1105	34-35	0.38051344354271066
1105	36-37	0.2887655952076216
1105	38-39	0.27240111476586293
1105	40-41	0.2980175225482924
1105	42-43	0.3281486992585201
1105	44-45	0.26832661647596723
1105	46-47	0.3274199102555144
1105	48-49	0.26546697610137215
1105	50-51	0.23157561260786963
1105	52-53	0.26065696993272525
1105	54-55	0.20350723173122987
1105	56-57	0.21473067225163334
1105	58-59	0.2799550587062356
1105	60-61	0.28784106871599136
1105	62-63	0.3564173250798177
1105	64-65	0.34791653823432256
1105	66-67	0.4044108982384671
1105	68-69	0.340219769187442
1105	70-71	0.38000892106686734
1105	72-73	0.40449905579740175
1105	74-75	0.43805733922859247
1105	76-77	0.36406375066946595
1105	78-79	0.4127007077004663
1105	80-81	0.40323085337998066
1105	82-83	0.42235177737073215
1105	84-85	0.39840802555478305
1105	86-87	0.36535498421072177
1105	88-89	0.4105354422751546
1105	90-91	0.39249520858174947
1105	92-93	0.4682629276752728
1105	94-95	0.5083520527391414
1105	96-97	0.3854251387208265
1105	98-99	0.41228821721119857
1105	100	0.45735900112650896
1106	1	0.12706895462884304
1106	2	0.19343280313379552
1106	3	0.15137833447919746
1106	4	0.14425489270576008
1106	5	0.13697452208358385
1106	6	0.19883079287328798
1106	7	0.15469540797750625
1106	8	0.25103201026863786
1106	9	0.25704032479804084
1106	10-11	0.22264581138788486
1106	12-13	0.26071327254045684
1106	14-15	0.3306727464781716
1106	16-17	0.342544364308182
1106	18-19	0.25355215054922553
1106	20-21	0.22900041021920003
1106	22-23	0.29463234575013075
1106	24-25	0.27546046784718214
1106	26-27	0.243511877647137
1106	28-29	0.3226578997015821
1106	30-31	0.33073181047363676
1106	32-33	0.3213206289488113
1106	34-35	0.33920807483689686
1106	36-37	0.32082851561953873
1106	38-39	0.28015496585488364
1106	40-41	0.2638649440217975
1106	42-43	0.18948965209301605
1106	44-45	0.23963732610815214
1106	46-47	0.2120873177070166
1106	48-49	0.20431502261207157
1106	50-51	0.18347833829921711
1106	52-53	0.17613060923665103
1106	54-55	0.1531545321657788
1106	56-57	0.25181207921369264
1106	58-59	0.2669921508611921
1106	60-61	0.26006701139776567
1106	62-63	0.3010742491030669
1106	64-65	0.24847568196641845
1106	66-67	0.2559339717018432
1106	68-69	0.30893649174367965
1106	70-71	0.3366701145241464
1106	72-73	0.24553043332648627
1106	74-75	0.21889953552334163
1106	76-77	0.17986917228848398
1106	78-79	0.18846571686106728
1106	80-81	0.16012775861905482
1106	82-83	0.22410833034016164
1106	84-85	0.2954538153174049
1106	86-87	0.333798873066538
1106	88-89	0.35237680518696735
1106	90-91	0.25734910200133143
1106	92-93	0.2621015159510307
1106	94-95	0.3016156233220464
1106	96-97	0.3869869049635213
1106	98-99	0.42861938110323194
1106	100	0.41513839937111285
1107	1	-0.07895428550020611
1107	2	0.052488974184640824
1107	3	-0.055296270699006556
1107	4	-0.0744070386561475
1107	5	-0.08295340079916258
1107	6	-0.176744954005855
1107	7	-0.1275830937736515
1107	8	-0.08461374345927197
1107	9	-0.1664788933255963
1107	10-11	-0.1877309874060984
1107	12-13	-0.09160751332750294
1107	14-15	-0.018817369230973213
1107	16-17	-0.03021188292557042
1107	18-19	0.021295952589852618
1107	20-21	0.0460982090571207
1107	22-23	0.06130564025476559
1107	24-25	0.12211972722227671
1107	26-27	0.16941513156804433
1107	28-29	0.0960717922953549
1107	30-31	0.08780907542555383
1107	32-33	0.14528457003502382
1107	34-35	0.05887218742773115
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2216	48-49	-0.8053048729632124
2216	50-51	-0.7490400279139493
2216	52-53	-0.7988232038838348
2216	54-55	-0.8449878764309844
2216	56-57	-0.7869259936543074
2216	58-59	-0.7507205121627933
2216	60-61	-0.8095368162437211
2216	62-63	-0.8883292604675077
2216	64-65	-0.9318107926016026
2216	66-67	-0.9213478793308809
2216	68-69	-1.017822772081935
2216	70-71	-1.0338582515752037
2216	72-73	-0.9751436906823869
2216	74-75	-0.9718253002671844
2216	76-77	-0.9457099287755355
2216	78-79	-0.9591194353901926
2216	80-81	-0.8946605446669906
2216	82-83	-0.8994233947666608
2216	84-85	-0.8892613518932215
2216	86-87	-0.9560368099702821
2216	88-89	-1.017741221626924
2216	90-91	-1.0400759610248436
2216	92-93	-1.0985422467443016
2216	94-95	-1.0945981484821026
2216	96-97	-1.2113324532295806
2216	98-99	-1.3766792645452952
2216	100	-1.370162897570797
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43664.0
3	8823.0
4	6492.0
5	6121.0
6	3880.0
7	3565.0
8	3938.0
9	4061.0
10	4249.0
11	4468.0
12	5313.0
13	5628.0
14	6114.0
15	6195.0
16	5833.0
17	6046.0
18	5715.0
19	5446.0
20	5203.0
21	5485.0
22	6558.0
23	11160.0
24	19725.0
25	24313.0
26	23685.0
27	16615.0
28	16293.0
29	19011.0
30	22141.0
31	25908.0
32	33726.0
33	44097.0
34	61813.0
35	99673.0
36	164371.0
37	335763.0
38	509488.0
39	260824.0
40	6359.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93193033271745	22.070416300005306	12.294114909235754	21.703538458041493
2	16.613034160280556	23.629042732822445	38.845629302506815	20.91229380439018
3	16.42219962430206	30.406151011954467	30.244658264786413	22.92699109895706
4	11.938942286877085	20.25993635537083	38.86933367969866	28.93178767805343
5	11.79065463047149	38.381986838117506	33.3034593237217	16.52389920768931
6	26.68455136538147	41.854632793617355	18.37255014444501	13.088265696556158
7	23.367403377707735	35.40959279387713	24.129460395873494	17.093543432541637
8	22.242637309350446	37.45173891442729	22.735287336789046	17.570336439433216
9	23.008915650392204	19.760932414455976	22.414683276309393	34.81546865884243
10-11	21.859092242399182	29.744821032145914	28.370915734818663	20.02517099063624
12-13	22.61046065456482	28.809879194398412	28.56482598949432	20.01483416154245
14-15	20.993585754009445	29.278364854348126	26.219962311163453	23.50808708047898
16-17	20.408445459967247	32.817862906586456	25.80302549787256	20.970666135573737
18-19	19.425580783672356	30.81990537742415	28.624276286664625	21.13023755223887
20-21	22.866906019281704	30.4338437526045	26.41936569753031	20.279884530583484
22-23	20.91735840438325	31.730736967206813	27.436055076357235	19.9158495520527
24-25	21.122633759109668	29.832494661108953	27.636757331301325	21.408114248480054
26-27	21.21517814523732	30.319651556856346	27.534606729654577	20.930563568251756
28-29	20.573131171655223	30.256656430860684	28.019003529675356	21.151208867808734
30-31	22.225995555704685	31.137559923843007	26.159131966129838	20.47731255432247
32-33	19.84955313508991	30.98897477056028	28.501587325640422	20.65988476870939
34-35	20.46267322306661	28.735113071921603	28.311844274316716	22.49036943069508
36-37	22.106256108741277	30.938887151050835	26.72890231534148	20.225954424866405
38-39	20.838208600458284	30.34966083294277	27.944075048626395	20.868055517972554
40-41	21.100634172582833	29.943466745176057	27.487441564443905	21.468457517797205
42-43	19.94989073268094	30.791709105393444	28.336035701567624	20.92236446035799
44-45	21.211389778553734	29.661693443203184	27.847634056767053	21.279282721476033
46-47	21.026219827012355	31.66200517166172	26.96846238855437	20.34331261277156
48-49	20.026307500641316	30.28745044004585	28.542204028440892	21.144038030871943
50-51	21.195992773961148	29.5247710473535	27.619926159321384	21.659310019363968
52-53	20.075393908955807	30.578342881821357	28.20433583978889	21.14192736943394
54-55	20.968420175325612	30.453732677693342	27.479810711552677	21.098036435428373
56-57	20.480884442909854	30.858682016406874	27.25286048744373	21.40757305323954
58-59	19.484219287981894	29.688996743087042	28.699610664143975	22.12717330478709
60-61	21.89502760636922	30.446994796948957	26.57777354442834	21.08020405225348
62-63	20.38726318304608	30.828491664102753	27.846315663574543	20.93792948927662
64-65	20.600034149428918	29.2681441841926	28.172061166985024	21.959760499393454
66-67	20.844459405486205	30.3357521152616	27.18864767215691	21.631140807095285
68-69	21.055227891903826	30.44493825503501	27.58650735321973	20.913326499841432
70-71	22.333043974278073	29.658229793663903	27.025666725476547	20.983059506581476
72-73	20.51446560758366	30.526712855876458	28.685647826938748	20.273173709601128
74-75	20.810715882240245	29.22608539411461	28.183283344932953	21.779915378712193
76-77	21.33762356840329	30.534722545436043	27.24357898906894	20.884074897091725
78-79	20.98885029565496	30.46320359440231	27.87358436854963	20.674361741393103
80-81	21.52284763946872	29.787494276860333	27.341968283794127	21.347689799876825
82-83	20.162515650702566	30.536506219285453	28.431227964879646	20.86975016513233
84-85	20.97954836410455	29.42668906147722	27.766182591309047	21.82757998310918
86-87	21.559436034915112	30.44383715552823	27.35768616608898	20.639040643467677
88-89	20.176733953295802	29.951764159891326	28.192948772590675	21.678553114222197
90-91	21.100637142613596	29.6373739820128	27.262153945237394	21.99983493013621
92-93	20.516469504545608	30.818609766208915	27.831222080425693	20.833698648819787
94-95	20.789086222232324	29.402650131689363	27.882424794456085	21.925838851622228
96-97	20.723350940618708	30.403984369926178	27.70425172645206	21.168412963003053
98-99	20.10751175106416	29.999864697016026	28.194300497103164	21.69832305481665
100	21.431621560917794	29.894923133958795	27.158378412021467	21.515076893101945
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17504.0
1	11697.5
2	4727.0
3	2859.5
4	1725.0
5	1154.5
6	987.5
7	1080.5
8	1272.5
9	1251.5
10	1001.0
11	778.5
12	719.5
13	747.5
14	771.5
15	826.0
16	936.5
17	1142.0
18	1475.0
19	1852.5
20	2407.0
21	3300.5
22	4509.0
23	6345.0
24	8653.0
25	11310.5
26	15247.5
27	20359.5
28	26291.0
29	32329.5
30	36953.0
31	41255.5
32	45509.0
33	49083.0
34	52449.0
35	55932.5
36	58940.5
37	61739.5
38	65471.5
39	68828.0
40	71429.5
41	72009.0
42	69618.5
43	66969.5
44	63790.0
45	59007.5
46	54770.0
47	52018.5
48	50589.5
49	50844.0
50	52027.5
51	50300.5
52	45731.5
53	42549.5
54	40606.5
55	38903.5
56	36717.0
57	33787.5
58	31823.5
59	29452.5
60	25853.5
61	22269.0
62	18244.5
63	14015.5
64	10619.0
65	8008.5
66	5974.0
67	4380.0
68	3095.5
69	2211.0
70	1572.0
71	1224.5
72	975.0
73	697.5
74	539.5
75	444.5
76	352.5
77	293.0
78	219.5
79	168.0
80	143.0
81	110.0
82	96.5
83	88.5
84	74.5
85	66.0
86	58.5
87	49.0
88	38.5
89	32.0
90	35.0
91	31.5
92	19.0
93	16.5
94	15.5
95	12.5
96	13.5
97	10.5
98	8.5
99	13.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.3295619241006143E-4
2	1.0823904810251536E-4
3	5.411952405125768E-5
4	1.0823904810251536E-4
5	1.0823904810251536E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	2.705976202562884E-5
64-65	2.705976202562884E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	8.117928607688653E-5
84-85	6.76494050640721E-4
86-87	0.0012718088152045557
88-89	0.003761306921562409
90-91	0.003220111681049832
92-93	0.0023812590582553378
94-95	0.002300079772178452
96-97	0.0022730200101528228
98-99	0.003084812870921688
100	0.0036260081114342646
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1847762.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.26982164121765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.42894880120964	67.55619280148618
2	9.00352746436202	13.914019226261157
3	1.7956566061080415	4.162501970485277
4	0.6147178402415293	1.8999655150054993
5	0.31493990654424353	1.2167675203187727
6	0.17750271524470784	0.8229361888674251
7	0.11775395789437311	0.6369179126831963
8	0.08787163503658318	0.5431860453279163
9	0.07135598422677991	0.4962297756813141
>10	0.35508836535507726	4.866915463461393
>50	0.02205568397395576	1.1589466686295715
>100	0.009950788938444019	1.2585417199027429
>500	4.20167243050282E-4	0.2053490181571072
>1k	1.4005574768342693E-4	0.15995038146835502
>5k	0.0	0.0
>10k+	7.002787384171347E-5	1.101579792264131
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20358	1.101765270635504	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	2.7059762025628843E-5	0.0
14-15	0.0	0.0	0.0	5.4119524051257685E-5	0.0
16-17	0.0	0.0	0.0	5.4119524051257685E-5	0.0
18-19	0.0	0.0	0.0	8.117928607688653E-5	0.0
20-21	0.0	2.7059762025628843E-5	0.0	2.976573822819173E-4	0.0
22-23	0.0	5.4119524051257685E-5	0.0	0.0011094502430507825	0.0
24-25	0.0	5.4119524051257685E-5	0.0	0.002056541913947792	0.0
26-27	0.0	5.4119524051257685E-5	0.0	0.0038695459696649246	0.0
28-29	0.0	5.4119524051257685E-5	0.0	0.007739091939329848	0.0
30-31	0.0	5.4119524051257685E-5	0.0	0.015532303402710956	0.0
32-33	0.0	5.4119524051257685E-5	0.0	0.029386901559832923	0.0
34-35	5.4119524051257685E-5	5.4119524051257685E-5	0.0	0.05144060761072043	0.0
36-37	5.4119524051257685E-5	5.4119524051257685E-5	0.0	0.08318170846678305	0.0
38-39	5.4119524051257685E-5	5.4119524051257685E-5	0.0	0.12626084961158418	0.0
40-41	5.4119524051257685E-5	5.4119524051257685E-5	0.0	0.18002859675650867	0.0
42-43	5.4119524051257685E-5	5.4119524051257685E-5	0.0	0.2319292203216648	0.0
44-45	5.4119524051257685E-5	5.4119524051257685E-5	0.0	0.29002652939068996	0.0
46-47	5.4119524051257685E-5	5.4119524051257685E-5	0.0	0.35840654802945404	0.0
48-49	5.4119524051257685E-5	5.4119524051257685E-5	0.0	0.42957372215685785	0.0
50-51	5.4119524051257685E-5	5.4119524051257685E-5	0.0	0.508155271079284	0.0
52-53	5.4119524051257685E-5	5.4119524051257685E-5	0.0	0.5819743018851995	0.0
54-55	5.4119524051257685E-5	5.4119524051257685E-5	0.0	0.6659136836887002	0.0
56-57	5.4119524051257685E-5	5.4119524051257685E-5	0.0	0.7597298786315554	0.0
58-59	5.4119524051257685E-5	5.4119524051257685E-5	0.0	0.860879269083356	0.0
60-61	5.4119524051257685E-5	5.4119524051257685E-5	0.0	0.972203130056793	0.0
62-63	5.4119524051257685E-5	5.4119524051257685E-5	0.0	1.0807398355415905	0.0
64-65	5.4119524051257685E-5	5.4119524051257685E-5	0.0	1.2026170037050226	0.0
66-67	5.4119524051257685E-5	5.4119524051257685E-5	0.0	1.3456819655345222	0.0
68-69	5.4119524051257685E-5	5.4119524051257685E-5	0.0	1.4996520114603504	0.0
70-71	5.4119524051257685E-5	5.4119524051257685E-5	0.0	1.6638235876698406	0.0
72-73	5.4119524051257685E-5	5.4119524051257685E-5	0.0	1.8531066230391144	0.0
74-75	5.4119524051257685E-5	5.4119524051257685E-5	0.0	2.049398136773026	0.0
76-77	5.4119524051257685E-5	5.4119524051257685E-5	0.0	2.2534287424462676	0.0
78-79	5.4119524051257685E-5	5.4119524051257685E-5	0.0	2.470583332701939	0.0
80-81	5.4119524051257685E-5	5.4119524051257685E-5	0.0	2.720940250963057	0.0
82-83	5.4119524051257685E-5	5.4119524051257685E-5	0.0	2.986694173816758	0.0
84-85	5.4119524051257685E-5	5.4119524051257685E-5	0.0	3.253286949293253	0.0
86-87	5.4119524051257685E-5	5.4119524051257685E-5	0.0	3.539552171762381	0.0
88	5.4119524051257685E-5	5.4119524051257685E-5	0.0	3.7672600692080476	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	315	0.0	41.77761	3
TATAACG	325	0.0	40.492146	2
TACCTGG	1760	0.0	39.522568	2
GTACCTG	1875	0.0	37.850513	1
TCTGTCG	720	0.0	35.902634	8
TAACGCA	390	0.0	33.743454	4
GTCTCGC	730	0.0	33.479317	1
GGTATCA	2910	0.0	32.94832	1
TCGCTCT	815	0.0	31.717663	4
GTATAAC	570	0.0	31.333204	1
GTATCAA	4355	0.0	30.865547	1
CTGTCGC	835	0.0	30.395086	9
CTCGCTC	860	0.0	28.41849	3
TCTCGCT	895	0.0	27.832289	2
ACCTGGG	2445	0.0	27.296415	3
CAACGCA	4785	0.0	26.323824	5
TCAACGC	4845	0.0	25.997831	4
ATCAACG	4870	0.0	25.96088	3
CGCTCTG	875	0.0	25.782753	5
GTACACG	165	9.3245035E-8	25.636261	1
>>END_MODULE