FastQCFastQC Report
Wed 25 May 2016
SRR2431357_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431357_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847762
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124540.6740045525343632No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104460.5653325482394378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91250.4938406569677264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75230.4071411794376116No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42150.22811379387605116No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27200.14720510541942092No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20190.10926731905948926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2450.051.795892
GGTATCA50450.049.0987631
GTATCAA95850.042.858841
ATAACGC2800.041.964263
TCTGTCG7450.041.0055778
TACCTGG16150.039.5789222
TAACGCA3500.036.2571264
GTACCTG18200.035.8975261
CTGTCGC8650.034.2302679
ATCAACG124000.032.9378853
TCAACGC124900.032.8510634
TATCAAC125100.032.6858332
CAACGCA125750.032.6281245
AACGCAG130650.031.620256
CGCTCTG6550.029.4190355
ACCTGGG21750.028.7402133
ACGCAGA145100.028.4389117
CGCAGAG144850.028.42318
TCGCTCT7650.028.2614214
GTATAAC5850.028.1211621
Produced by FastQC (version 0.11.5)