##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431357_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1847762
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70925855169659	34.0	31.0	34.0	31.0	34.0
2	32.82789828993128	34.0	31.0	34.0	31.0	34.0
3	32.90770131651154	34.0	31.0	34.0	31.0	34.0
4	36.31666632390968	37.0	37.0	37.0	35.0	37.0
5	36.27125571366875	37.0	37.0	37.0	35.0	37.0
6	36.30632841242541	37.0	37.0	37.0	35.0	37.0
7	36.177955277790105	37.0	37.0	37.0	35.0	37.0
8	36.23099403494606	37.0	37.0	37.0	35.0	37.0
9	38.046767927904135	39.0	39.0	39.0	35.0	39.0
10-11	38.04154458204033	39.0	39.0	39.0	35.0	39.0
12-13	38.01770330811003	39.0	39.0	39.0	35.0	39.0
14-15	39.49154490675747	41.0	39.0	41.0	36.0	41.0
16-17	39.503423601091484	41.0	39.0	41.0	36.5	41.0
18-19	39.58250034365898	41.0	40.0	41.0	37.0	41.0
20-21	39.555763404594316	41.0	40.0	41.0	37.0	41.0
22-23	39.427963125121096	41.0	39.0	41.0	36.0	41.0
24-25	39.38118734988597	41.0	39.0	41.0	36.0	41.0
26-27	39.26823557362907	41.0	39.0	41.0	35.0	41.0
28-29	39.154504746823456	41.0	39.0	41.0	35.0	41.0
30-31	38.94230479899468	41.0	39.0	41.0	35.0	41.0
32-33	38.65014514856351	41.0	38.0	41.0	35.0	41.0
34-35	38.47447642066457	41.0	38.0	41.0	35.0	41.0
36-37	38.30780749901773	41.0	38.0	41.0	35.0	41.0
38-39	38.1590245388746	41.0	38.0	41.0	34.0	41.0
40-41	38.04932751079414	41.0	38.0	41.0	34.0	41.0
42-43	37.89965239029702	40.5	38.0	41.0	34.0	41.0
44-45	37.672802557905186	40.0	38.0	41.0	33.0	41.0
46-47	37.315318206565564	40.0	38.0	41.0	32.5	41.0
48-49	37.00550097902219	40.0	38.0	41.0	31.0	41.0
50-51	36.85538559619691	40.0	37.0	41.0	31.0	41.0
52-53	36.73681377796491	40.0	37.0	41.0	31.0	41.0
54-55	36.59382837183577	40.0	37.0	41.0	31.0	41.0
56-57	36.44520154651952	40.0	36.0	41.0	30.0	41.0
58-59	36.280400830842936	40.0	36.0	41.0	30.0	41.0
60-61	36.08317088456197	39.5	35.5	41.0	30.0	41.0
62-63	35.82701370631066	39.0	35.0	41.0	29.0	41.0
64-65	35.5496662990147	39.0	35.0	41.0	28.5	41.0
66-67	35.26889204345581	38.5	35.0	41.0	28.0	41.0
68-69	34.98977844549244	37.5	35.0	40.5	28.0	41.0
70-71	34.71644345970964	37.0	35.0	40.0	28.0	41.0
72-73	34.45329755671997	37.0	35.0	39.5	28.0	41.0
74-75	34.15940012837151	36.0	35.0	39.0	28.0	41.0
76-77	33.84447293536722	36.0	35.0	39.0	28.0	41.0
78-79	33.53191293034493	35.0	35.0	37.5	27.5	40.0
80-81	33.22312749152759	35.0	34.0	37.0	27.0	39.0
82-83	32.91740305299059	35.0	34.0	37.0	26.5	39.0
84-85	32.65190132711896	35.0	34.0	36.0	26.0	39.0
86-87	32.42168147196446	35.0	34.0	36.0	26.0	37.0
88-89	32.20071010227508	35.0	34.0	36.0	26.0	37.0
90-91	31.991515952812108	35.0	34.0	35.0	25.0	37.0
92-93	31.827641492789656	35.0	34.0	35.0	25.0	36.0
94-95	31.671474464784968	35.0	34.0	35.0	25.0	36.0
96-97	31.515640001255573	35.0	34.0	35.0	24.0	36.0
98-99	31.383723661380632	35.0	34.0	35.0	23.5	36.0
100	31.267871078634585	35.0	34.0	35.0	20.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22874920388157705
1101	2	0.15057209771944002
1101	3	0.17976373344774998
1101	4	0.09459810297272497
1101	5	0.12302568883877996
1101	6	0.15112959227990785
1101	7	0.13890143783427789
1101	8	0.1499899161034719
1101	9	0.22573798164480507
1101	10-11	0.25547704521276415
1101	12-13	0.16431134070298725
1101	14-15	0.26480946632181457
1101	16-17	0.2765089157270282
1101	18-19	0.2097710299196578
1101	20-21	0.21335925489049146
1101	22-23	0.2500935666517137
1101	24-25	0.23832809537127986
1101	26-27	0.2200402646690094
1101	28-29	0.24851667691078205
1101	30-31	0.1711291567734392
1101	32-33	0.11646602232547565
1101	34-35	0.10671151024983772
1101	36-37	0.11224697132972494
1101	38-39	0.12244734794920475
1101	40-41	0.18324384400889215
1101	42-43	0.11078703787882205
1101	44-45	0.06054325744189981
1101	46-47	0.013759248082351405
1101	48-49	0.001314482605103251
1101	50-51	-0.040519900894338434
1101	52-53	-0.07606987851406899
1101	54-55	-0.03847491802496705
1101	56-57	-0.05469583614797102
1101	58-59	0.019392970890223182
1101	60-61	0.006377967706626464
1101	62-63	-0.030949392474916237
1101	64-65	0.014782148369015147
1101	66-67	0.012578934898229477
1101	68-69	0.004462878232999401
1101	70-71	0.03732764807444511
1101	72-73	-0.07773227328285515
1101	74-75	-0.03084628432858949
1101	76-77	0.007025568474354316
1101	78-79	0.007254680179741513
1101	80-81	-0.08457650396847782
1101	82-83	-0.01724800429676776
1101	84-85	-0.12225561109873695
1101	86-87	-0.12134780088328512
1101	88-89	-0.0622797236099899
1101	90-91	-0.10933573129948826
1101	92-93	-0.0881235330380612
1101	94-95	-0.1482080478862251
1101	96-97	-0.10535660667779823
1101	98-99	-0.12304378406755845
1101	100	-0.1827737815291144
1102	1	0.1341150758263936
1102	2	0.17030294192397122
1102	3	0.2207803776222903
1102	4	0.09318184866173596
1102	5	0.14987004331471354
1102	6	0.12213424065297573
1102	7	0.16522022625832733
1102	8	0.17778193889549243
1102	9	0.23144873288187284
1102	10-11	0.2069026712436468
1102	12-13	0.18838653938345118
1102	14-15	0.3449268002549317
1102	16-17	0.27567933108165477
1102	18-19	0.2703591990604579
1102	20-21	0.30934965824931737
1102	22-23	0.3652543853651622
1102	24-25	0.3708232220769361
1102	26-27	0.3225837494493362
1102	28-29	0.23338172414425173
1102	30-31	0.2645089738374651
1102	32-33	0.30310232461967246
1102	34-35	0.25994737769622844
1102	36-37	0.27334533690177665
1102	38-39	0.2938939666326661
1102	40-41	0.342553710555606
1102	42-43	0.3535262041706204
1102	44-45	0.3546169566471846
1102	46-47	0.40050613667134627
1102	48-49	0.460173381990316
1102	50-51	0.3778329256163744
1102	52-53	0.31823623934468515
1102	54-55	0.3187473041972595
1102	56-57	0.3101782079366018
1102	58-59	0.3487380751037463
1102	60-61	0.26970042984487463
1102	62-63	0.30132943493548936
1102	64-65	0.30480164154639766
1102	66-67	0.3123202754290375
1102	68-69	0.3374322139654993
1102	70-71	0.3334301372032442
1102	72-73	0.3206314244229418
1102	74-75	0.33993306789813005
1102	76-77	0.29692622824344284
1102	78-79	0.296646642992755
1102	80-81	0.29684087494890576
1102	82-83	0.3284826149864841
1102	84-85	0.3545141444868136
1102	86-87	0.30949565390753975
1102	88-89	0.3327468920745176
1102	90-91	0.31855938041140774
1102	92-93	0.3968532250965886
1102	94-95	0.3273212668536125
1102	96-97	0.3633808352175407
1102	98-99	0.4091975564632584
1102	100	0.42256435012480864
1103	1	0.12312301817085824
1103	2	0.12855636154450423
1103	3	0.11330280739137777
1103	4	0.091166111053262
1103	5	0.14012228629162138
1103	6	0.06493024006465475
1103	7	0.052274204784581
1103	8	0.0988887193043766
1103	9	0.17965655756196952
1103	10-11	0.1371523403126318
1103	12-13	0.13663885590057845
1103	14-15	0.16333722825905284
1103	16-17	0.2234595193446296
1103	18-19	0.200527238478017
1103	20-21	0.19753027304560788
1103	22-23	0.21219033182788394
1103	24-25	0.16189396777483722
1103	26-27	0.12510066370143136
1103	28-29	0.10494886637104628
1103	30-31	0.17516495265788024
1103	32-33	0.17942935800693505
1103	34-35	0.18822653639603715
1103	36-37	0.1912722379166354
1103	38-39	0.1963130723835178
1103	40-41	0.1966039385303091
1103	42-43	0.110225351103999
1103	44-45	0.07012622271572155
1103	46-47	0.11001268175150614
1103	48-49	0.21026457187118552
1103	50-51	0.20007957953607303
1103	52-53	0.24821086796654157
1103	54-55	0.19617560247641563
1103	56-57	0.2543030792412111
1103	58-59	0.2125081148154635
1103	60-61	0.2170790884711451
1103	62-63	0.18182031594740522
1103	64-65	0.21095842930930786
1103	66-67	0.2760465587059855
1103	68-69	0.3115758380278564
1103	70-71	0.26151036959036134
1103	72-73	0.25452427631292807
1103	74-75	0.2431096379819664
1103	76-77	0.11076318956127551
1103	78-79	0.07265708570491825
1103	80-81	0.16643971301332527
1103	82-83	0.2772614795253858
1103	84-85	0.22573748615469924
1103	86-87	0.19014795571663257
1103	88-89	0.21200009107437268
1103	90-91	0.23140575487824577
1103	92-93	0.23771070413762985
1103	94-95	0.1948819375404831
1103	96-97	0.1614379024333097
1103	98-99	0.09835883017997915
1103	100	0.05436314406509268
1104	1	0.05275128237536819
1104	2	0.09587691306701629
1104	3	0.13964165112976445
1104	4	0.014303727531647326
1104	5	-0.0016541625430761542
1104	6	0.0805073650949879
1104	7	0.03031137621657365
1104	8	0.047925215686092315
1104	9	0.11875700117666099
1104	10-11	0.128300519931841
1104	12-13	0.08160421930741535
1104	14-15	0.14636626401094333
1104	16-17	0.17794087242312884
1104	18-19	0.17542968548156068
1104	20-21	0.21782473240765654
1104	22-23	0.19095917757782388
1104	24-25	0.19239192293803598
1104	26-27	0.17523077015606958
1104	28-29	0.06488087456328628
1104	30-31	0.15417432533359232
1104	32-33	0.1804705675710352
1104	34-35	0.223942136213644
1104	36-37	0.2116929739147082
1104	38-39	0.1385375026174671
1104	40-41	0.14163609111027853
1104	42-43	0.15958004456459918
1104	44-45	0.22340587177548343
1104	46-47	0.30794486604380467
1104	48-49	0.2804449599069727
1104	50-51	0.2785798368855694
1104	52-53	0.2593504143871641
1104	54-55	0.3229086669827339
1104	56-57	0.20648281871332586
1104	58-59	0.2043692849646419
1104	60-61	0.20229892363861524
1104	62-63	0.1706502741486844
1104	64-65	0.17860920425699334
1104	66-67	0.27098096142967165
1104	68-69	0.18366648231788218
1104	70-71	0.13011093955327624
1104	72-73	0.1079075094386468
1104	74-75	0.0816347497804557
1104	76-77	0.09383224154874625
1104	78-79	0.11683830269957696
1104	80-81	0.13459373328488056
1104	82-83	0.06878032831770753
1104	84-85	0.13317835840841497
1104	86-87	0.15486716558567792
1104	88-89	0.21067139456566508
1104	90-91	0.2143942017114142
1104	92-93	0.16068209932980793
1104	94-95	0.1378579428320741
1104	96-97	0.1152406975566258
1104	98-99	0.21323169809848608
1104	100	0.20716245512725706
1105	1	-0.021185667044441914
1105	2	0.02597152567666683
1105	3	0.06789156861199075
1105	4	-0.0729480339167452
1105	5	-0.041778650670430295
1105	6	-0.0904610145591036
1105	7	-0.4280735671756517
1105	8	-0.4232880347445729
1105	9	-0.4105042985104106
1105	10-11	-0.33658882168709425
1105	12-13	-0.22396428735989815
1105	14-15	-0.22013250959410158
1105	16-17	-0.13856641538372116
1105	18-19	-0.15916991668361646
1105	20-21	-0.0872223522946598
1105	22-23	-0.11365527195570735
1105	24-25	-0.05118612371745712
1105	26-27	-0.0724348223215685
1105	28-29	-0.10246299188737851
1105	30-31	-0.15166943498113028
1105	32-33	-0.17030230035037164
1105	34-35	-0.15627536373678907
1105	36-37	-0.09621912511595809
1105	38-39	-0.23889239809294338
1105	40-41	-0.1443472000602597
1105	42-43	-0.09856581595877856
1105	44-45	-0.13661656839475
1105	46-47	-0.28825314921410694
1105	48-49	-0.22264413538326977
1105	50-51	-0.1937558594303539
1105	52-53	-0.29629587184630424
1105	54-55	-0.30277917555352474
1105	56-57	-0.3006958634748571
1105	58-59	-0.32969590524612613
1105	60-61	-0.324358400732649
1105	62-63	-0.3619098413645361
1105	64-65	-0.25379648929472154
1105	66-67	-0.334917922510094
1105	68-69	-0.31174583539946354
1105	70-71	-0.32884280792016085
1105	72-73	-0.1985184677409677
1105	74-75	-0.3115808309755792
1105	76-77	-0.29071335958306577
1105	78-79	-0.41941134885962583
1105	80-81	-0.41594548977671053
1105	82-83	-0.3798848664417491
1105	84-85	-0.3101917026255876
1105	86-87	-0.38124901419693913
1105	88-89	-0.37690803244021964
1105	90-91	-0.33682703224135935
1105	92-93	-0.33628420600812703
1105	94-95	-0.34160336770186106
1105	96-97	-0.3120582973010322
1105	98-99	-0.25033036110204065
1105	100	-0.3351698938243892
1106	1	0.10792502737520238
1106	2	0.11243996625844943
1106	3	0.09022672745080484
1106	4	0.0707170982670462
1106	5	0.12128247730676378
1106	6	0.11814763278101736
1106	7	0.13207634254119682
1106	8	0.06702223504662186
1106	9	0.12145444903408276
1106	10-11	0.15533984800885037
1106	12-13	0.13706869410064115
1106	14-15	0.18190200304223936
1106	16-17	0.19300920750388428
1106	18-19	0.2296540708553323
1106	20-21	0.21805127510380373
1106	22-23	0.19195112988037977
1106	24-25	0.2101579708384378
1106	26-27	0.22424619705127213
1106	28-29	0.24569513260415476
1106	30-31	0.35305761557081894
1106	32-33	0.30697147917354073
1106	34-35	0.2406550917797432
1106	36-37	0.31848851290482827
1106	38-39	0.2342376162597546
1106	40-41	0.21417777827840467
1106	42-43	0.19826856902444945
1106	44-45	0.1445278118924307
1106	46-47	0.22097731093232653
1106	48-49	0.23895121420763843
1106	50-51	0.19643519030144319
1106	52-53	0.1972809614308133
1106	54-55	0.23636584630624213
1106	56-57	0.25374644790611
1106	58-59	0.2702633350525687
1106	60-61	0.19169414207743785
1106	62-63	0.15947536013555208
1106	64-65	0.21214063659240168
1106	66-67	0.2589137209078345
1106	68-69	0.21073111428668767
1106	70-71	0.22846756439035687
1106	72-73	0.22514514318825718
1106	74-75	0.27520839589639223
1106	76-77	0.3079313183408843
1106	78-79	0.24999440280548413
1106	80-81	0.24888657694683758
1106	82-83	0.25013444687717623
1106	84-85	0.17536299309043102
1106	86-87	0.1847068793416895
1106	88-89	0.2307145277650875
1106	90-91	0.22445885779178099
1106	92-93	0.29702688724477966
1106	94-95	0.27779734067364004
1106	96-97	0.27009813066095134
1106	98-99	0.2540411788705761
1106	100	0.3212142494116499
1107	1	0.04844216753510722
1107	2	0.1023269281096546
1107	3	0.09858897379637455
1107	4	0.005515307906804878
1107	5	0.004796115138368862
1107	6	-0.025619853726865927
1107	7	0.04365316476843617
1107	8	0.05974737612400105
1107	9	0.10474298860860642
1107	10-11	0.161756501208842
1107	12-13	0.13803301970074955
1107	14-15	0.15336736092190506
1107	16-17	0.16458949755494956
1107	18-19	0.20498436431960698
1107	20-21	0.1384077485280315
1107	22-23	0.1538575070768502
1107	24-25	0.11940617542664
1107	26-27	0.05825578085531902
1107	28-29	0.12156522251189728
1107	30-31	0.11988630432878722
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2216	36-37	-0.9130520150018313
2216	38-39	-0.861266270688084
2216	40-41	-0.850036315558846
2216	42-43	-0.8075349815168238
2216	44-45	-0.8404892191900828
2216	46-47	-0.8042171418369222
2216	48-49	-0.6381241394281076
2216	50-51	-0.5966429528827248
2216	52-53	-0.7611004766492684
2216	54-55	-0.7758715165293637
2216	56-57	-0.7633378501565886
2216	58-59	-0.8691781525042614
2216	60-61	-0.7985633031463735
2216	62-63	-0.8310090678363053
2216	64-65	-0.7282180130098865
2216	66-67	-0.6191900099972685
2216	68-69	-0.6263703012679258
2216	70-71	-0.6347295961545214
2216	72-73	-0.6684514774875296
2216	74-75	-0.6279843050802896
2216	76-77	-0.558807152431875
2216	78-79	-0.5968689569522638
2216	80-81	-0.6499185285421873
2216	82-83	-0.6444972952164747
2216	84-85	-0.6288634383234779
2216	86-87	-0.5248189564374428
2216	88-89	-0.5159862137118907
2216	90-91	-0.49753754083291923
2216	92-93	-0.5837409930735653
2216	94-95	-0.5528507677823846
2216	96-97	-0.7063873418109132
2216	98-99	-0.6309294651155213
2216	100	-0.6102207325709372
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24.0
3	154.0
4	90.0
5	176.0
6	333.0
7	607.0
8	1065.0
9	1580.0
10	2263.0
11	3429.0
12	6027.0
13	9372.0
14	8103.0
15	8448.0
16	10411.0
17	15870.0
18	19647.0
19	10080.0
20	6007.0
21	5359.0
22	5093.0
23	5460.0
24	5987.0
25	7238.0
26	8595.0
27	10684.0
28	13035.0
29	15972.0
30	19617.0
31	24019.0
32	30088.0
33	38702.0
34	54088.0
35	90060.0
36	136307.0
37	309649.0
38	596946.0
39	347527.0
40	19650.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.026440873914936	21.377423827962204	13.495172640742501	23.100962657380357
2	14.311258701066478	22.601125036665977	40.82430529472952	22.26331096753803
3	14.510806092152958	29.88834011453906	32.25131700431984	23.349536788988146
4	10.216008157133054	18.625778532105723	39.992845189824365	31.165368120936858
5	10.985776306688848	36.39440577303787	34.934693970327345	17.685123949945936
6	26.52387049847329	40.14683709265587	19.27320726370604	14.056085145164799
7	22.673807557466816	33.45382143371278	26.176423154064217	17.695947854756184
8	22.757638700222216	33.80126877812186	24.53373324053639	18.907359281119536
9	23.772812732375705	15.126028135658165	24.80779451033196	36.29336462163417
10-11	22.107933813986865	26.689530361594187	30.35986236322643	20.84267346119251
12-13	23.638379834632385	25.654494464113885	31.21974042111484	19.48738528013889
14-15	21.516921551585106	25.682907214240796	29.889563699221007	22.910607534953094
16-17	19.385640574922526	29.82762390394434	29.488808623621445	21.297926897511694
18-19	18.356530765325836	26.690720991123317	34.45321962460534	20.499528618945515
20-21	21.340735441036237	26.498786099075534	32.69576926032682	19.464709199561415
22-23	19.574836558860458	27.712662899943712	33.76539702125817	18.947103519937656
24-25	19.813850333958325	25.963889800653277	33.74752842697958	20.47473143840882
26-27	19.888486147360105	26.482686398746587	33.69715370429752	19.931673749595795
28-29	19.286015425305358	26.457629659535986	34.071432011919406	20.18492290323925
30-31	21.064825776716773	27.3594700955957	32.23166594460932	19.344038183078204
32-33	18.716923109396173	27.100880643248694	34.48123014574737	19.70096610160777
34-35	19.27814719358844	25.279731899016163	33.845205454244336	21.596915453151063
36-37	21.05026446578885	27.487231333012573	32.08303137923252	19.37947282196606
38-39	19.81958212609605	26.916067208580564	33.16604522191112	20.098305443412265
40-41	20.180484235501943	26.70631424216971	32.44240394523775	20.670797577090603
42-43	19.075931435622465	27.530824609897703	33.173409317535764	20.21983463694407
44-45	20.368295779633	26.437014884015948	32.39852274707564	20.796166589275412
46-47	20.372987231721098	28.61786844646112	31.02020128765213	19.988943034165647
48-49	19.62655718434443	27.491170227620326	31.968700987051115	20.91357160098413
50-51	20.715303362607713	26.907762018349366	30.859438316111916	21.517496302931
52-53	19.76009356183318	28.027743832809637	31.344810641197295	20.867351964159887
54-55	20.615936636621196	27.946345700593422	30.427816458350808	21.00990120443457
56-57	20.100624893959072	28.13283806525059	30.50980555577354	21.256731485016804
58-59	19.098261022041918	26.911944893037592	31.96562633946547	22.024167745455017
60-61	21.368976120011222	27.727267684312984	29.79766587715237	21.106090318523425
62-63	19.991481586914333	28.099506321701607	31.12884668047075	20.78016541091331
64-65	20.178843379179785	26.607836940038815	31.40813048433727	21.80518919644413
66-67	20.524994696257938	27.806816066225338	30.313375388039088	21.354813849477637
68-69	20.679924784135935	27.702498473141773	30.650464605978573	20.96711213674372
70-71	21.943804994949197	27.04426223253279	29.915304023620383	21.09662874889763
72-73	20.243267523920856	27.86858141750011	31.527795817638655	20.360355240940383
74-75	20.581057977532467	26.61764443239364	30.907982917445327	21.893314672628566
76-77	20.973921617918812	27.964426048562135	30.0256554367418	21.035996896777252
78-79	20.651610652879768	27.908809903100778	30.609480910374785	20.830098533644666
80-81	21.174346215896907	27.234090455220233	30.04462741842345	21.546935910459407
82-83	19.786415333284626	27.93166806025723	31.1962689656852	21.08564764077294
84-85	20.589567602438432	26.856588358560497	30.4987054483738	22.055138590627273
86-87	20.954021293378595	27.97585722152393	30.044148109262174	21.025973375835303
88-89	19.587846184444988	27.444448804142375	30.897004842421726	22.07070016899091
90-91	20.399946312298976	27.20643191647233	29.963095117612326	22.43052665361637
92-93	19.828668861998526	28.24598685344577	30.61543528105218	21.30990900350353
94-95	20.088020899010306	26.76324575441498	30.610615220839694	22.538118125735018
96-97	20.036802013286607	27.781650407934084	30.52521343814691	21.6563341406324
98-99	19.332930314771207	27.29037306998939	30.911911579820135	22.464785035419272
100	20.571134459170935	27.181330644572064	29.837673074123323	22.409861822133678
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2191.0
1	1467.5
2	4670.0
3	6698.5
4	2993.0
5	1213.5
6	1864.5
7	4199.0
8	6744.5
9	6717.0
10	4248.0
11	2275.5
12	1949.5
13	2064.5
14	2329.0
15	2595.0
16	2766.5
17	3062.5
18	3441.5
19	3816.0
20	4424.5
21	5239.0
22	6389.0
23	8227.5
24	10344.0
25	12897.5
26	16622.0
27	21430.0
28	27174.5
29	32972.5
30	37458.0
31	41608.5
32	45687.5
33	48976.5
34	52334.5
35	55922.5
36	58615.0
37	60971.5
38	64542.5
39	67973.5
40	70925.0
41	71591.5
42	69233.5
43	66751.5
44	63030.0
45	57884.5
46	53936.5
47	51798.5
48	50303.0
49	50229.0
50	51532.5
51	49767.0
52	44954.5
53	41419.0
54	38778.0
55	36843.5
56	34829.5
57	32247.5
58	30413.5
59	27992.0
60	24555.5
61	20788.0
62	16877.5
63	12996.5
64	9523.5
65	6797.0
66	4901.0
67	3451.5
68	2316.5
69	1543.5
70	1042.5
71	730.0
72	505.5
73	342.5
74	262.5
75	209.0
76	142.5
77	93.5
78	73.0
79	41.0
80	20.5
81	8.0
82	5.5
83	5.5
84	3.0
85	1.5
86	1.5
87	1.5
88	1.5
89	0.5
90	1.0
91	3.0
92	2.5
93	2.0
94	4.0
95	5.0
96	6.5
97	9.5
98	5.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009092080040611291
2	0.0
3	4.3295619241006143E-4
4	0.002922454298767915
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.0823904810251536E-4
24-25	8.117928607688653E-5
26-27	5.14135478486948E-4
28-29	0.001028270956973896
30-31	0.0017588845316658748
32-33	0.004248382638023729
34-35	0.005411952405125768
36-37	0.004058964303844326
38-39	0.0027871554886397705
40-41	0.003571888587383007
42-43	5.14135478486948E-4
44-45	4.3295619241006143E-4
46-47	0.0031389323949729455
48-49	0.0019483028658452766
50-51	7.847330987432364E-4
52-53	0.0
54-55	3.7883666835880376E-4
56-57	4.6001595443569035E-4
58-59	5.411952405125768E-4
60-61	8.117928607688653E-5
62-63	0.0
64-65	0.0
66-67	5.411952405125768E-4
68-69	7.306135746919788E-4
70-71	0.0019212431038196478
72-73	1.0823904810251536E-4
74-75	0.0015694661974864728
76-77	2.976573822819172E-4
78-79	9.470916708970096E-4
80-81	0.001028270956973896
82-83	9.200319088713807E-4
84-85	9.741514329226383E-4
86-87	2.4353785823065958E-4
88-89	0.0015965259595121018
90-91	0.0021106614379990496
92-93	0.0028953945367422864
94-95	0.001028270956973896
96-97	0.0020024223898965346
98-99	0.0021106614379990496
100	0.0038424862076392955
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1847762.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.27828002249935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.95197474920187	62.98412874158807
2	10.008284083831793	14.66779687279499
3	1.9820675282340119	4.3572749807230515
4	0.7041189592870574	2.063865050711512
5	0.35430273248279887	1.2981347421805605
6	0.2199889590764747	0.9672247527038438
7	0.13976565335305705	0.7169250678753021
8	0.11028997271422065	0.6465487603381178
9	0.07606089787135648	0.5016250595681986
>10	0.4102595356312075	5.46905311359978
>50	0.028333990466769314	1.459731101838797
>100	0.01314984364094272	1.7514911258690258
>500	8.123176996931012E-4	0.4085596283484944
>1k	2.9538825443385414E-4	0.5675461433270198
>5k	1.4769412721692707E-4	0.900887508972943
>10k+	1.4769412721692707E-4	1.2392073495603313
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12454	0.6740045525343632	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10446	0.5653325482394378	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9125	0.4938406569677264	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7523	0.4071411794376116	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4215	0.22811379387605116	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2720	0.14720510541942092	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2019	0.10926731905948926	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.0823904810251537E-4	0.0
4	0.0	0.0	0.0	1.0823904810251537E-4	0.0
5	0.0	0.0	0.0	1.0823904810251537E-4	0.0
6	0.0	0.0	0.0	1.0823904810251537E-4	0.0
7	0.0	0.0	0.0	1.0823904810251537E-4	0.0
8	0.0	0.0	0.0	1.0823904810251537E-4	0.0
9	0.0	0.0	0.0	1.0823904810251537E-4	0.0
10-11	0.0	0.0	0.0	1.352988101281442E-4	0.0
12-13	0.0	0.0	0.0	2.705976202562884E-4	0.0
14-15	0.0	0.0	0.0	2.705976202562884E-4	0.0
16-17	0.0	0.0	0.0	2.705976202562884E-4	0.0
18-19	0.0	0.0	0.0	2.976573822819173E-4	0.0
20-21	0.0	0.0	0.0	5.14135478486948E-4	0.0
22-23	0.0	0.0	0.0	0.0012988685772301846	0.0
24-25	0.0	0.0	0.0	0.002191840724075936	0.0
26-27	0.0	0.0	0.0	0.004221322875998099	0.0
28-29	0.0	0.0	0.0	0.008036749321611766	0.0
30-31	0.0	0.0	0.0	0.015938199833095388	0.0
32-33	0.0	0.0	0.0	0.030009276086422385	0.0
34-35	0.0	0.0	0.0	0.05246887856769432	0.0
36-37	0.0	0.0	0.0	0.08442645751996199	0.0
38-39	0.0	0.0	0.0	0.1278844353331219	0.0
40-41	0.0	0.0	0.0	0.18232867652868714	0.0
42-43	0.0	0.0	0.0	0.23495991366853525	0.0
44-45	0.0	0.0	0.0	0.29365253750212417	0.0
46-47	0.0	0.0	0.0	0.3624655123332983	0.0
48-49	0.0	0.0	0.0	0.43468801717970174	0.0
50-51	0.0	0.0	0.0	0.5143790163451787	0.0
52-53	0.0	0.0	0.0	0.5897133938245294	0.0
54-55	0.0	0.0	0.0	0.6750057637293114	0.0
56-57	0.0	0.0	0.0	0.7703643651076275	0.0
58-59	0.0	0.0	0.0	0.8731373412809659	0.0
60-61	0.0	0.0	0.0	0.9856788915455562	0.0
62-63	0.0	0.0	0.0	1.0955144656075837	0.0
64-65	0.0	0.0	0.0	1.2192587573507843	0.0
66-67	0.0	0.0	0.0	1.3640014244258731	0.0
68-69	0.0	0.0	0.0	1.5208127453643923	0.0
70-71	0.0	0.0	0.0	1.6870679232498558	0.0
72-73	0.0	0.0	0.0	1.879300472679923	0.0
74-75	0.0	0.0	0.0	2.0792450542872944	0.0
76-77	0.0	0.0	0.0	2.286928727833996	0.0
78-79	0.0	0.0	0.0	2.50871053739605	0.0
80-81	0.0	0.0	0.0	2.7640464518698837	0.0
82-83	0.0	0.0	0.0	3.0348334904603513	0.0
84-85	0.0	0.0	0.0	3.306811158579947	0.0
86-87	0.0	0.0	0.0	3.600626054654225	0.0
88	0.0	0.0	0.0	3.833340008074633	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	245	0.0	51.79589	2
GGTATCA	5045	0.0	49.098763	1
GTATCAA	9585	0.0	42.85884	1
ATAACGC	280	0.0	41.96426	3
TCTGTCG	745	0.0	41.005577	8
TACCTGG	1615	0.0	39.578922	2
TAACGCA	350	0.0	36.257126	4
GTACCTG	1820	0.0	35.897526	1
CTGTCGC	865	0.0	34.230267	9
ATCAACG	12400	0.0	32.937885	3
TCAACGC	12490	0.0	32.851063	4
TATCAAC	12510	0.0	32.685833	2
CAACGCA	12575	0.0	32.628124	5
AACGCAG	13065	0.0	31.62025	6
CGCTCTG	655	0.0	29.419035	5
ACCTGGG	2175	0.0	28.740213	3
ACGCAGA	14510	0.0	28.438911	7
CGCAGAG	14485	0.0	28.4231	8
TCGCTCT	765	0.0	28.261421	4
GTATAAC	585	0.0	28.121162	1
>>END_MODULE