FastQCFastQC Report
Wed 25 May 2016
SRR2431356_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431356_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975639
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239260.8040625895815992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45150.049.6538161
GTATCAA81250.040.3760721
TCAACGC97750.032.118244
ATCAACG98750.031.9357763
CAACGCA104400.030.1624345
AACGCAG109300.028.5092266
TATCAAC119400.027.042352
ACGCAGA121150.025.4491027
CGCAGAG120700.025.3103478
GTGGTAT20800.024.8554341
GTACATG192300.024.1962431
TACATGG182150.023.9447782
TGGTATC22050.022.5938012
GTACACG2504.3837645E-1022.5596961
GCAGAGT131750.022.4384089
ACATGGG189900.022.2250843
CATGGGG95150.020.3507544
CATGGGA73250.019.1847254
ATACCGT4950.018.9896456
CAGAGTA130200.018.9333110-11
Produced by FastQC (version 0.11.5)