##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431356_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2975639
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.011932563056202	33.0	31.0	34.0	27.0	34.0
2	31.101012589228734	34.0	31.0	34.0	27.0	34.0
3	31.123237731458687	34.0	31.0	34.0	27.0	34.0
4	34.2558600018349	37.0	35.0	37.0	30.0	37.0
5	34.45796415492605	37.0	35.0	37.0	31.0	37.0
6	34.4329130650593	37.0	35.0	37.0	31.0	37.0
7	34.40138269460778	37.0	35.0	37.0	31.0	37.0
8	34.35142199709037	37.0	35.0	37.0	31.0	37.0
9	35.760783146073834	39.0	37.0	39.0	30.0	39.0
10-11	35.8284654153276	39.0	37.0	39.0	30.0	39.0
12-13	35.785135562479184	39.0	37.0	39.0	30.0	39.0
14-15	37.13368288290347	41.0	38.0	41.0	30.5	41.0
16-17	37.160518631460334	41.0	38.0	41.0	31.0	41.0
18-19	37.19853080296367	41.0	38.0	41.0	30.5	41.0
20-21	37.26351734871065	41.0	38.0	41.0	30.0	41.0
22-23	37.29741662211041	41.0	38.0	41.0	30.0	41.0
24-25	37.33897038585662	41.0	38.0	41.0	30.0	41.0
26-27	37.3651813946517	41.0	38.0	41.0	30.0	41.0
28-29	37.35293310109191	40.0	38.0	41.0	30.0	41.0
30-31	37.27878835436691	40.0	38.0	41.0	30.0	41.0
32-33	37.206507072934585	40.0	38.0	41.0	30.0	41.0
34-35	37.15211942712136	40.0	38.0	41.0	30.0	41.0
36-37	37.05373518091408	40.0	38.0	41.0	30.0	41.0
38-39	36.947022135413604	40.0	38.0	41.0	30.0	41.0
40-41	36.85861171331603	40.0	37.5	41.0	29.5	41.0
42-43	36.7274326623626	40.0	37.0	41.0	29.0	41.0
44-45	36.58320414539533	40.0	37.0	41.0	29.0	41.0
46-47	36.45354510409361	40.0	37.0	41.0	29.0	41.0
48-49	36.307073875560846	40.0	36.0	41.0	29.0	41.0
50-51	36.16294382483897	40.0	36.0	41.0	28.0	41.0
52-53	35.992183863701214	40.0	35.0	41.0	28.0	41.0
54-55	35.82719341963188	39.5	35.0	41.0	28.0	41.0
56-57	35.61464394706481	39.0	35.0	41.0	28.0	41.0
58-59	35.37292023662817	39.0	35.0	41.0	28.0	41.0
60-61	35.17553002901225	39.0	35.0	41.0	27.0	41.0
62-63	34.99487084958895	38.5	35.0	40.5	27.0	41.0
64-65	34.740738711920365	38.0	34.0	40.0	26.5	41.0
66-67	34.421173233715514	37.0	34.0	40.0	26.0	41.0
68-69	34.099880563468886	37.0	34.0	40.0	26.0	41.0
70-71	33.84822285230164	36.0	34.0	39.0	26.0	41.0
72-73	33.60005884450365	36.0	34.0	39.0	26.0	41.0
74-75	33.270365121575566	35.5	33.5	39.0	26.0	40.5
76-77	32.93429562524217	35.0	33.0	37.5	25.5	40.0
78-79	32.60981574041743	35.0	33.0	37.0	25.0	39.0
80-81	32.285060116499345	35.0	33.0	37.0	25.0	39.0
82-83	31.964848390547374	35.0	33.0	36.0	24.0	39.0
84-85	31.691037790538438	35.0	33.0	36.0	23.5	37.0
86-87	31.441539615524597	35.0	32.0	35.5	23.0	37.0
88-89	31.20253666523392	35.0	32.0	35.0	21.5	37.0
90-91	30.997453992234945	35.0	32.0	35.0	20.0	36.0
92-93	30.81071695860956	35.0	32.0	35.0	19.5	36.0
94-95	30.59855395765414	35.0	32.0	35.0	17.5	36.0
96-97	30.41003562596135	35.0	32.0	35.0	11.5	36.0
98-99	30.287023224255364	35.0	31.5	35.0	2.0	35.0
100	30.23697901526361	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4082812038349317
1101	2	0.3732822655283847
1101	3	0.4233077483957892
1101	4	0.33650547048999613
1101	5	0.2901445091505792
1101	6	0.3591924528926853
1101	7	0.2897517762731141
1101	8	0.26526873614121627
1101	9	0.35812994167009293
1101	10-11	0.39832101252923735
1101	12-13	0.3516700565571327
1101	14-15	0.46605149712515015
1101	16-17	0.3693675009012267
1101	18-19	0.3803490615997944
1101	20-21	0.35621219901025114
1101	22-23	0.3854706615171608
1101	24-25	0.3953012487183116
1101	26-27	0.4952757311680429
1101	28-29	0.5113696800104819
1101	30-31	0.5741555062828212
1101	32-33	0.4808510821379741
1101	34-35	0.48387044321933814
1101	36-37	0.555880619300865
1101	38-39	0.4878949128337027
1101	40-41	0.47063809474385465
1101	42-43	0.47642183472518695
1101	44-45	0.4638053087720806
1101	46-47	0.3996722837947857
1101	48-49	0.38333076986377534
1101	50-51	0.34479418715080357
1101	52-53	0.3810778179141181
1101	54-55	0.3318224326489272
1101	56-57	0.404525171894484
1101	58-59	0.34496537380397285
1101	60-61	0.4099926357546835
1101	62-63	0.4077613296366138
1101	64-65	0.37156222393042526
1101	66-67	0.3293495625160645
1101	68-69	0.42897255582900584
1101	70-71	0.4278968731153583
1101	72-73	0.5121925398727001
1101	74-75	0.47562806746933006
1101	76-77	0.416842700708294
1101	78-79	0.4130987760738165
1101	80-81	0.3150088162718774
1101	82-83	0.41892934841492746
1101	84-85	0.43196280370339224
1101	86-87	0.4527265323494909
1101	88-89	0.4475476206429789
1101	90-91	0.4467822108476618
1101	92-93	0.42693059634574126
1101	94-95	0.5462039935670333
1101	96-97	0.5354129330025366
1101	98-99	0.5417688455554419
1101	100	0.5511541150137376
1102	1	0.08782772772971725
1102	2	0.10801358568490471
1102	3	0.22890214906407635
1102	4	0.06639611066798068
1102	5	0.09644783818686165
1102	6	0.10300988580197412
1102	7	0.0895242682006554
1102	8	0.11768095084218544
1102	9	0.13602621829620176
1102	10-11	0.21901381420394728
1102	12-13	0.16063080424883225
1102	14-15	0.1587077911143524
1102	16-17	0.07386291078133667
1102	18-19	0.06522517949493079
1102	20-21	0.10903151616053464
1102	22-23	0.049945382882924605
1102	24-25	0.10380470907091421
1102	26-27	0.16068801849863945
1102	28-29	0.16932977840784247
1102	30-31	0.17540163887635885
1102	32-33	0.18918717855515155
1102	34-35	0.1933121375350666
1102	36-37	0.22869876077416507
1102	38-39	0.2074881164490634
1102	40-41	0.21335314829180874
1102	42-43	0.22761348294902461
1102	44-45	0.23543989087356465
1102	46-47	0.18556024906470014
1102	48-49	0.20217522026756996
1102	50-51	0.08337311673577119
1102	52-53	0.0912060322915309
1102	54-55	0.06365008568915442
1102	56-57	0.030036220950577786
1102	58-59	0.10136054653254689
1102	60-61	0.07009518685229921
1102	62-63	0.14480580642027263
1102	64-65	0.16379453333168925
1102	66-67	0.18602148086384318
1102	68-69	0.1746059724556588
1102	70-71	0.14527724130144293
1102	72-73	0.1277840572174611
1102	74-75	0.1892381925744786
1102	76-77	0.18473345602489388
1102	78-79	0.2229896810220353
1102	80-81	0.302697425589308
1102	82-83	0.29528055713961265
1102	84-85	0.294711138140741
1102	86-87	0.2604282247170886
1102	88-89	0.29022430679819067
1102	90-91	0.28801814614364574
1102	92-93	0.28989250805483024
1102	94-95	0.28283170014428904
1102	96-97	0.2639093392931855
1102	98-99	0.22700276762603266
1102	100	0.2282131347274614
1103	1	0.3388572088750479
1103	2	0.3773228881867716
1103	3	0.3653396959469859
1103	4	0.44613745689287754
1103	5	0.43158618596198295
1103	6	0.3868652350029791
1103	7	0.4856958250372685
1103	8	0.497722395513712
1103	9	0.491422406830722
1103	10-11	0.571726991108271
1103	12-13	0.49217472586838085
1103	14-15	0.5524527351728921
1103	16-17	0.5579278028260077
1103	18-19	0.536065235153032
1103	20-21	0.5402043558881218
1103	22-23	0.5576918085430407
1103	24-25	0.5119320804165568
1103	26-27	0.5539165780189208
1103	28-29	0.558266631238368
1103	30-31	0.5415090105137281
1103	32-33	0.5065506714236889
1103	34-35	0.5737464600933109
1103	36-37	0.5842834972171858
1103	38-39	0.5297228288244256
1103	40-41	0.47724848351851534
1103	42-43	0.4007300543374228
1103	44-45	0.3900787074478913
1103	46-47	0.4028177151194967
1103	48-49	0.4622261867878166
1103	50-51	0.34892012924975546
1103	52-53	0.39304194133038095
1103	54-55	0.4779334207083039
1103	56-57	0.4229528454719471
1103	58-59	0.4275537487168819
1103	60-61	0.46041045999808006
1103	62-63	0.45609021342454525
1103	64-65	0.4490363732245015
1103	66-67	0.43874137710390926
1103	68-69	0.49738976933946333
1103	70-71	0.5419288360915431
1103	72-73	0.40304487385348153
1103	74-75	0.5105757779799802
1103	76-77	0.5116488074593022
1103	78-79	0.41881993864519274
1103	80-81	0.37801095672936924
1103	82-83	0.3798425604677149
1103	84-85	0.35382676595266815
1103	86-87	0.34962214594208163
1103	88-89	0.3065660421706653
1103	90-91	0.37734489978864616
1103	92-93	0.4286143899662136
1103	94-95	0.39752950579097046
1103	96-97	0.36841067251939563
1103	98-99	0.3349461463423822
1103	100	0.18989666070964972
1104	1	0.2071662079436294
1104	2	0.2251805783850429
1104	3	0.15474559759590534
1104	4	0.17210129985877387
1104	5	0.15674092693245
1104	6	0.13793644407586925
1104	7	0.13433257741624516
1104	8	0.16664258050149527
1104	9	0.2307901770747236
1104	10-11	0.19195516254688272
1104	12-13	0.19912011008661779
1104	14-15	0.18107665847533383
1104	16-17	0.23903484070753223
1104	18-19	0.2284228052034507
1104	20-21	0.20520299930650765
1104	22-23	0.309219003528014
1104	24-25	0.2746471961114736
1104	26-27	0.2799114415983581
1104	28-29	0.31026954215800373
1104	30-31	0.3286163645318041
1104	32-33	0.28279901447243816
1104	34-35	0.3061331667111844
1104	36-37	0.24824257943127748
1104	38-39	0.2759722142570311
1104	40-41	0.2778885801933413
1104	42-43	0.30237187162989443
1104	44-45	0.27706915823100786
1104	46-47	0.29404738364485894
1104	48-49	0.2569087860369592
1104	50-51	0.25357402530766393
1104	52-53	0.2503727509695324
1104	54-55	0.20765055833137325
1104	56-57	0.23896844610344203
1104	58-59	0.2564567606017647
1104	60-61	0.25301852720515683
1104	62-63	0.26030754566775016
1104	64-65	0.3185189094744274
1104	66-67	0.2796988625547243
1104	68-69	0.32992310537949265
1104	70-71	0.34125985147035465
1104	72-73	0.3033395468728415
1104	74-75	0.28249569051098433
1104	76-77	0.2640872614786005
1104	78-79	0.24592466731575513
1104	80-81	0.2665470780099284
1104	82-83	0.27598386415233733
1104	84-85	0.2898166993073943
1104	86-87	0.21840321146138209
1104	88-89	0.2475640368932801
1104	90-91	0.25357658636457714
1104	92-93	0.27999021119988754
1104	94-95	0.32928957320606145
1104	96-97	0.2814129056614263
1104	98-99	0.346262426245481
1104	100	0.38961044993119387
1105	1	0.12520442383085495
1105	2	0.15948771585735244
1105	3	0.09429858809585667
1105	4	0.16029700294087945
1105	5	0.10256855164144696
1105	6	0.09422877012924857
1105	7	0.13460476408483402
1105	8	0.1673054391762392
1105	9	0.19728039561569233
1105	10-11	0.20939332450564052
1105	12-13	0.22426161159133784
1105	14-15	0.30808172211884255
1105	16-17	0.36081446387790095
1105	18-19	0.23946394436508456
1105	20-21	0.2099523299568773
1105	22-23	0.24762790793532474
1105	24-25	0.2210653392525188
1105	26-27	0.1928880089688363
1105	28-29	0.22563327971176506
1105	30-31	0.14124643312666052
1105	32-33	0.2148118502105305
1105	34-35	0.1877815805255878
1105	36-37	0.19327509755580508
1105	38-39	0.24401416280825572
1105	40-41	0.21593977337654735
1105	42-43	0.18143760578013257
1105	44-45	0.11526612480975729
1105	46-47	0.12123125663967471
1105	48-49	0.05516182411030712
1105	50-51	0.12670248174440957
1105	52-53	0.12668195178679298
1105	54-55	0.11163504849081107
1105	56-57	0.06516147172918352
1105	58-59	0.07799650445254258
1105	60-61	0.17572803764559808
1105	62-63	0.19197143081063928
1105	64-65	0.18551614001700045
1105	66-67	0.18727026645100153
1105	68-69	0.17045944900756638
1105	70-71	0.15178126849022533
1105	72-73	0.16943382546511288
1105	74-75	0.2247654590146837
1105	76-77	0.19889311871365578
1105	78-79	0.17966898497812878
1105	80-81	0.12477977638945248
1105	82-83	0.17980227823101558
1105	84-85	0.26195830414705057
1105	86-87	0.2537232048530349
1105	88-89	0.16179383680572101
1105	90-91	0.15978130323753348
1105	92-93	0.22832791511364547
1105	94-95	0.2250070077076387
1105	96-97	0.2537057253169728
1105	98-99	0.2598936988340981
1105	100	0.26267524001524833
1106	1	0.08313238301792936
1106	2	0.17006276181605173
1106	3	0.04708629452037627
1106	4	0.011310139779723727
1106	5	-0.016116199508410034
1106	6	0.03462094019147344
1106	7	0.020241580740751886
1106	8	0.1221903189433533
1106	9	0.04405513728268318
1106	10-11	0.10192045545129247
1106	12-13	0.06383713136889213
1106	14-15	-0.003490919247028046
1106	16-17	0.07377745189346996
1106	18-19	0.10539650385922528
1106	20-21	0.05528126392505328
1106	22-23	0.08656583398538231
1106	24-25	0.1186271202313236
1106	26-27	0.20258019121744297
1106	28-29	0.17170305078301595
1106	30-31	0.23280102327118612
1106	32-33	0.1563028243833955
1106	34-35	0.1927440429896734
1106	36-37	0.1643990417720218
1106	38-39	0.11771562532624102
1106	40-41	0.1874820699888815
1106	42-43	0.21228396552444906
1106	44-45	0.20179355965751
1106	46-47	0.23154503662971848
1106	48-49	0.23916950694561478
1106	50-51	0.23408833609781965
1106	52-53	0.23879955518548002
1106	54-55	0.2596829862315744
1106	56-57	0.2736045925785362
1106	58-59	0.24638239690193586
1106	60-61	0.24229823415812746
1106	62-63	0.36709265614213393
1106	64-65	0.3096675843419874
1106	66-67	0.2732686053793216
1106	68-69	0.2686615726857582
1106	70-71	0.29241523444063944
1106	72-73	0.25568890646346176
1106	74-75	0.2635900603217678
1106	76-77	0.37311722139619974
1106	78-79	0.30157157548369895
1106	80-81	0.26981307141756616
1106	82-83	0.27584637780093857
1106	84-85	0.26116118346783423
1106	86-87	0.2452470368159574
1106	88-89	0.26533242923254363
1106	90-91	0.22384793312136608
1106	92-93	0.26539441902097494
1106	94-95	0.3123432517765181
1106	96-97	0.27974257474409114
1106	98-99	0.29577194881694524
1106	100	0.301761237163678
1107	1	0.07544528746367973
1107	2	0.06830113418827821
1107	3	0.0790808423815399
1107	4	0.08883259814957256
1107	5	0.03788537475352882
1107	6	-0.024030071898380356
1107	7	0.0465748260314669
1107	8	0.05683443669634869
1107	9	0.022474317165745106
1107	10-11	0.040735654507642494
1107	12-13	0.01104261007058227
1107	14-15	0.06819924754369566
1107	16-17	0.03197190550901752
1107	18-19	0.061225437563130924
1107	20-21	0.07206004110320663
1107	22-23	0.07997455005396148
1107	24-25	-0.0033091469219783676
1107	26-27	0.09381814413666234
1107	28-29	0.14039330311513964
1107	30-31	0.12094588538445095
1107	32-33	0.1169049120438288
1107	34-35	0.12455056859806746
1107	36-37	0.18856936520856493
1107	38-39	0.13139146318176387
1107	40-41	0.06115280162299541
1107	42-43	0.0914255012777474
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2216	78-79	-1.0714210183371193
2216	80-81	-1.0013096595517936
2216	82-83	-0.9857312646969518
2216	84-85	-0.938477706079027
2216	86-87	-0.9352483797223989
2216	88-89	-0.9585461425908157
2216	90-91	-0.9164364704759187
2216	92-93	-1.0014483615334626
2216	94-95	-0.9984576893925237
2216	96-97	-1.06981964742549
2216	98-99	-1.1861385769586121
2216	100	-1.1211973656108754
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58239.0
3	12684.0
4	9451.0
5	8532.0
6	5704.0
7	5095.0
8	5663.0
9	5983.0
10	5900.0
11	6432.0
12	7221.0
13	8067.0
14	8301.0
15	8297.0
16	7974.0
17	7802.0
18	7541.0
19	7637.0
20	7179.0
21	7495.0
22	9700.0
23	17079.0
24	30342.0
25	40029.0
26	37553.0
27	24620.0
28	23474.0
29	28183.0
30	33657.0
31	39056.0
32	50709.0
33	68833.0
34	101155.0
35	167168.0
36	300462.0
37	587662.0
38	836087.0
39	372555.0
40	6118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.31443425425876	22.408632793727715	13.677977436710881	21.59895551530264
2	15.34444041916389	22.893476961915393	39.727077016760774	22.03500560215994
3	17.029787551514143	30.411350301565477	30.09407391151951	22.464788235400867
4	11.762497752580542	19.86167658331751	39.222754134831725	29.153071529270225
5	12.210452948089468	40.696267255537386	34.38185882091208	12.711420975461069
6	27.001494468919113	41.53286067295126	18.52620563179875	12.939439226330881
7	24.431223007898474	36.06650537918074	23.569492132614204	15.932779480306584
8	23.812028273590983	37.63668912794865	21.027617933492603	17.523664664967757
9	24.885075104876634	19.11623016098391	21.52337699566379	34.47531773847567
10-11	22.77025203662138	29.952222026932702	28.121270758986554	19.156255177459364
12-13	22.982122495369904	28.995368725843424	27.946249528252586	20.076259250534086
14-15	21.380802577194345	29.06958807839257	26.177570599121736	23.37203874529135
16-17	20.774865499477592	32.261978015478356	26.36028765586148	20.602868829182572
18-19	20.53505146289587	31.099404195199753	28.41295600709629	19.952588334808084
20-21	22.93964422431619	29.686934470209593	27.45237241479897	19.921048890675248
22-23	21.941220020304883	30.215022722850453	27.756643194957455	20.087114061887213
24-25	21.45320719348012	30.107096324520548	27.466184574136847	20.97351190786248
26-27	21.137342264972332	30.537508078096838	27.5216180457374	20.803531611193428
28-29	21.151389667899902	29.971680032423286	28.11423025440922	20.762700045267586
30-31	21.76448487198884	30.199345417908557	27.385664054006554	20.65050565609605
32-33	20.62210839419701	30.20860393347446	28.295266999793995	20.874020672534538
34-35	21.566779437962737	29.53098477335456	27.867342107023063	21.034893681659636
36-37	21.923139870125375	29.915440011372347	27.369146593387168	20.79227352511511
38-39	21.1886253675261	29.854226268710686	27.820360601538024	21.13678776222519
40-41	21.414845685246092	29.92797513408044	27.646515588752536	21.010663591920927
42-43	20.966925087350987	30.066449592843757	28.171495265386692	20.795130054418564
44-45	21.121412913327188	29.5441080050369	27.809741033774593	21.524738047861316
46-47	21.607409366525978	29.90996219635513	27.623192867145512	20.85943556997337
48-49	21.194035970089114	29.53300114698053	27.974798018173573	21.29816486475678
50-51	21.546582095475962	29.806169363958464	27.20442567125918	21.44282286930639
52-53	20.96055670731564	30.061425461892387	27.83603790647992	21.141979924312054
54-55	21.27711056347897	29.75251702239418	27.653690518238268	21.316681895888582
56-57	21.410493678836716	29.99204540604556	27.457245317728397	21.140215597389332
58-59	21.365982231043485	29.240089271581667	27.7289180576004	21.66501043977445
60-61	21.852012290469375	29.557718527012184	27.366995794852805	21.223273387665643
62-63	20.823275269614356	29.846076758639068	28.020166424757843	21.31048154698873
64-65	21.23271673748059	29.362449544450786	27.75753375997559	21.647299958093036
66-67	21.28878872739603	29.487498315487866	27.532439250863426	21.691273706252673
68-69	21.365444531409892	29.702813412514082	27.508562026509264	21.42318002956676
70-71	21.865908465375	29.450565071905565	27.332868671233307	21.35065779148613
72-73	21.102274166994047	29.52009635577434	27.91254248247183	21.46508699475978
74-75	21.21278824481061	29.18961271847828	27.843196032852102	21.754403003859004
76-77	21.345482432512817	29.78056141890868	27.493573649222906	21.3803824993556
78-79	21.136334078159347	29.69992327698353	27.711526835076434	21.45221580978069
80-81	21.446455945505477	29.83890684301873	27.356649673675076	21.35798753780071
82-83	20.84296222238092	29.988901537905743	27.97207321120264	21.196063028510697
84-85	21.25663999048267	29.197894694745692	27.533942023282165	22.01152329148947
86-87	21.47823122267466	29.778967527091567	27.395887926600437	21.34691332363334
88-89	20.965277215321326	29.528129189367036	27.763961420099015	21.742632175212627
90-91	21.29265925427046	29.560525831138783	27.298780463214268	21.84803445137648
92-93	20.85287080475422	29.824533692612764	28.03039939534589	21.29219610728712
94-95	21.264567718761953	28.99571714613525	27.774159442879487	21.96555569222331
96-97	21.20821570277805	29.883557722666847	27.33996517334603	21.568261401209067
98-99	20.524782428679355	29.556361524625817	27.90326982070026	22.015586225994564
100	21.01308125271378	29.511358549461008	27.41827073662872	22.057289461196497
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20337.0
1	13586.0
2	5413.0
3	3237.0
4	2102.0
5	1519.5
6	1243.5
7	1229.5
8	1448.5
9	1495.0
10	1209.5
11	981.0
12	952.5
13	981.5
14	1033.0
15	1143.5
16	1304.0
17	1576.0
18	1933.5
19	2485.5
20	3322.5
21	4468.5
22	6259.0
23	8981.5
24	12475.5
25	16463.0
26	21759.5
27	28599.5
28	36786.5
29	45475.0
30	52429.0
31	59060.5
32	66230.0
33	71896.0
34	77397.0
35	85503.5
36	93283.5
37	99107.0
38	108094.0
39	115338.0
40	120026.5
41	123435.0
42	122342.5
43	121210.0
44	117806.0
45	110560.0
46	104282.0
47	98775.5
48	90394.5
49	87062.5
50	87649.5
51	82712.5
52	74994.0
53	68680.0
54	64281.5
55	60991.0
56	55785.5
57	48787.0
58	44065.5
59	40432.0
60	35912.0
61	30230.0
62	24975.5
63	20302.5
64	16152.5
65	12989.0
66	10370.0
67	7899.0
68	5845.5
69	4325.0
70	3166.5
71	2487.0
72	1962.5
73	1476.5
74	1165.5
75	938.5
76	658.0
77	474.0
78	360.5
79	264.0
80	217.5
81	172.0
82	122.5
83	106.0
84	93.5
85	89.5
86	93.0
87	72.5
88	66.0
89	62.5
90	50.5
91	43.5
92	35.0
93	32.5
94	33.5
95	21.5
96	13.5
97	14.5
98	18.0
99	22.0
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.0245604389510954E-4
2	3.360622709945662E-5
3	0.0
4	1.3442490839782648E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.680311354972831E-5
82-83	5.040934064918494E-5
84-85	4.5368406584266437E-4
86-87	0.00119302106203071
88-89	0.003360622709945662
90-91	0.0027893168492548995
92-93	0.0017979331498209294
94-95	0.001781130036271201
96-97	0.0014954771059258196
98-99	0.002117192307265767
100	0.0027893168492548995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2975639.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.44561676654855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.85853993318426	43.383276903049854
2	14.127122832324515	15.948283228146948
3	4.331494638455349	7.334816591658313
4	1.7762369962161093	4.010431710999194
5	0.8591812879916058	2.4248508857481874
6	0.47419096847601017	1.6059601008453221
7	0.30769303029341366	1.2157546008776028
8	0.1972614973119584	0.890763750405308
9	0.15261084829797344	0.7752792111700757
>10	0.7743048890505569	8.075146492694937
>50	0.07531693935029542	2.952223192602233
>100	0.05804585236850572	6.540174364026184
>500	0.005612141402111032	2.1903706127639198
>1k	0.0023284416453666734	1.8463601625428934
>5k	0.0	0.0
>10k+	5.97036319324788E-5	0.806308192469177
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23926	0.8040625895815992	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.680311354972831E-5	0.0
12-13	0.0	0.0	0.0	3.360622709945662E-5	0.0
14-15	0.0	0.0	0.0	3.360622709945662E-5	0.0
16-17	0.0	0.0	0.0	3.360622709945662E-5	0.0
18-19	0.0	0.0	0.0	1.3442490839782648E-4	0.0
20-21	0.0	0.0	0.0	1.848342490470114E-4	0.0
22-23	0.0	0.0	0.0	5.713058606907626E-4	0.0
24-25	0.0	0.0	0.0	0.0010417930400831552	0.0
26-27	0.0	0.0	0.0	0.0019155549446690273	0.0
28-29	0.0	0.0	0.0	0.0036462756402910433	0.0
30-31	0.0	0.0	0.0	0.008334344320665241	0.0
32-33	0.0	0.0	0.0	0.016971144685225592	0.0
34-35	0.0	0.0	0.0	0.03160665658703895	0.0
36-37	0.0	0.0	0.0	0.05219047068545613	0.0
38-39	0.0	0.0	0.0	0.0797643800205603	0.0
40-41	0.0	0.0	0.0	0.10728788001501527	0.0
42-43	0.0	0.0	0.0	0.13481138000947024	0.0
44-45	0.0	0.0	0.0	0.16981226553355433	0.0
46-47	0.0	0.0	0.0	0.21351716387639763	0.0
48-49	0.0	0.0	0.0	0.2579950054425285	0.0
50-51	0.0	0.0	0.0	0.306018303967652	0.0
52-53	0.0	0.0	0.0	0.3542264367418225	0.0
54-55	0.0	0.0	0.0	0.40633289185953003	0.0
56-57	0.0	0.0	0.0	0.4685884275612734	0.0
58-59	0.0	0.0	0.0	0.542085246227785	0.0
60-61	0.0	0.0	0.0	0.6221352791786907	0.0
62-63	0.0	0.0	0.0	0.7010595035217646	0.0
64-65	0.0	0.0	0.0	0.7821009201721043	0.0
66-67	0.0	0.0	0.0	0.8810376527529045	0.0
68-69	0.0	0.0	0.0	0.9965422552937369	0.0
70-71	1.680311354972831E-5	0.0	0.0	1.128950790065596	0.0
72-73	3.360622709945662E-5	0.0	0.0	1.2709707057878996	0.0
74-75	3.360622709945662E-5	0.0	0.0	1.4204680070398323	0.0
76-77	3.360622709945662E-5	0.0	0.0	1.5745189520637415	0.0
78-79	3.360622709945662E-5	0.0	0.0	1.7518926186946735	0.0
80-81	3.360622709945662E-5	0.0	0.0	1.9571594538181545	0.0
82-83	3.360622709945662E-5	0.0	0.0	2.1751126396716804	0.0
84-85	3.360622709945662E-5	0.0	0.0	2.3960903859641576	0.0
86-87	3.360622709945662E-5	0.0	0.0	2.6367445782233663	0.0
88	3.360622709945662E-5	0.0	0.0	2.829375471957452	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4515	0.0	49.653816	1
GTATCAA	8125	0.0	40.376072	1
TCAACGC	9775	0.0	32.11824	4
ATCAACG	9875	0.0	31.935776	3
CAACGCA	10440	0.0	30.162434	5
AACGCAG	10930	0.0	28.509226	6
TATCAAC	11940	0.0	27.04235	2
ACGCAGA	12115	0.0	25.449102	7
CGCAGAG	12070	0.0	25.310347	8
GTGGTAT	2080	0.0	24.855434	1
GTACATG	19230	0.0	24.196243	1
TACATGG	18215	0.0	23.944778	2
TGGTATC	2205	0.0	22.593801	2
GTACACG	250	4.3837645E-10	22.559696	1
GCAGAGT	13175	0.0	22.438408	9
ACATGGG	18990	0.0	22.225084	3
CATGGGG	9515	0.0	20.350754	4
CATGGGA	7325	0.0	19.184725	4
ATACCGT	495	0.0	18.989645	6
CAGAGTA	13020	0.0	18.93331	10-11
>>END_MODULE