FastQCFastQC Report
Wed 25 May 2016
SRR2431356_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431356_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975639
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240170.8071207562476497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182330.6127423387043925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127910.4298572508291496No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54370.18271705673974564No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37250.12518319594547592No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36510.12269633514011612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86700.063.2182661
GTATCAA177050.047.1530951
ATCAACG234900.035.3956263
TCAACGC235500.035.28434
GTGGTAT25950.035.1420861
CAACGCA242150.034.314165
TATCAAC250150.033.2753642
AACGCAG251400.033.1637736
TGGTATC28750.031.2248462
ACGCAGA281200.029.5824077
CGCAGAG282300.029.4005478
GCAGAGT298550.027.6271219
TATAACG2601.8189894E-1225.3080962
TACATGG172200.023.9097882
GTACATG185150.023.6368261
GAGTACT196350.023.25345412-13
ACATGGG174850.022.8485183
AGAGTAC291050.022.57434510-11
GTATCGA1252.4222233E-422.5633931
CAGAGTA297700.022.52000610-11
Produced by FastQC (version 0.11.5)