##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431356_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2975639
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78184215222344	34.0	31.0	34.0	31.0	34.0
2	32.883687167697424	34.0	31.0	34.0	31.0	34.0
3	32.95888110083246	34.0	31.0	34.0	31.0	34.0
4	36.35510591170502	37.0	37.0	37.0	35.0	37.0
5	36.32307111178473	37.0	37.0	37.0	35.0	37.0
6	36.36671148617154	37.0	37.0	37.0	35.0	37.0
7	36.23822143747948	37.0	37.0	37.0	35.0	37.0
8	36.291004385948696	37.0	37.0	37.0	35.0	37.0
9	38.106691033421725	39.0	39.0	39.0	37.0	39.0
10-11	38.09850237209554	39.0	39.0	39.0	36.0	39.0
12-13	38.082278125807605	39.0	39.0	39.0	36.0	39.0
14-15	39.534975512822626	41.0	39.5	41.0	37.0	41.0
16-17	39.551645881775315	41.0	39.5	41.0	37.0	41.0
18-19	39.65444178544507	41.0	40.0	41.0	37.0	41.0
20-21	39.65690394567352	41.0	40.0	41.0	37.0	41.0
22-23	39.577726666440384	41.0	40.0	41.0	37.0	41.0
24-25	39.54959270933067	41.0	40.0	41.0	36.0	41.0
26-27	39.45576765192283	41.0	39.5	41.0	36.0	41.0
28-29	39.36627326097016	41.0	39.0	41.0	36.0	41.0
30-31	39.156810520362185	41.0	39.0	41.0	35.5	41.0
32-33	38.912333115677	41.0	38.5	41.0	35.0	41.0
34-35	38.79499915816401	41.0	38.0	41.0	35.0	41.0
36-37	38.67109837584465	41.0	38.0	41.0	35.0	41.0
38-39	38.536481239827815	41.0	38.0	41.0	35.0	41.0
40-41	38.41992358616082	41.0	38.0	41.0	35.0	41.0
42-43	38.249327623411304	41.0	38.0	41.0	35.0	41.0
44-45	37.991604828408285	40.5	38.0	41.0	34.0	41.0
46-47	37.588039409350394	40.0	38.0	41.0	33.0	41.0
48-49	37.22106865113678	40.0	38.0	41.0	33.0	41.0
50-51	37.02919188113881	40.0	37.0	41.0	32.0	41.0
52-53	36.90649739434118	40.0	37.0	41.0	32.0	41.0
54-55	36.74895022548098	40.0	36.5	41.0	31.5	41.0
56-57	36.57212602066312	40.0	36.0	41.0	31.0	41.0
58-59	36.37348633352366	40.0	35.5	41.0	31.0	41.0
60-61	36.163670055406584	39.0	35.0	41.0	31.0	41.0
62-63	35.922629055473465	39.0	35.0	41.0	30.5	41.0
64-65	35.652635618769615	39.0	35.0	41.0	30.0	41.0
66-67	35.34788964655995	38.0	35.0	41.0	29.5	41.0
68-69	35.06457570962069	37.0	35.0	40.0	29.0	41.0
70-71	34.80010730468313	37.0	35.0	40.0	29.0	41.0
72-73	34.52770480558966	36.0	35.0	39.0	29.0	41.0
74-75	34.23117454771899	36.0	35.0	39.0	29.0	41.0
76-77	33.920871113733895	35.5	35.0	38.5	29.0	40.5
78-79	33.611270217926304	35.0	35.0	37.0	29.0	39.5
80-81	33.31627240401137	35.0	35.0	37.0	29.0	39.0
82-83	33.01874168875996	35.0	34.0	36.5	29.0	39.0
84-85	32.775759089056166	35.0	34.0	36.0	28.5	37.5
86-87	32.56846731071881	35.0	34.0	36.0	28.0	37.0
88-89	32.3570617941222	35.0	34.0	35.5	27.0	37.0
90-91	32.171292787868424	35.0	34.0	35.0	27.0	36.0
92-93	32.037011714122585	35.0	34.0	35.0	27.0	36.0
94-95	31.903135763444425	35.0	34.0	35.0	26.0	36.0
96-97	31.77156217538485	35.0	34.0	35.0	26.0	36.0
98-99	31.655633126195752	35.0	34.0	35.0	25.0	36.0
100	31.55151112080464	35.0	34.0	35.0	25.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.21550666701062937
1101	2	0.20341293627294732
1101	3	0.2068462357450329
1101	4	0.13329216198437166
1101	5	0.12678156854647682
1101	6	0.16063100691901155
1101	7	0.19836849438303972
1101	8	0.21039008785734836
1101	9	0.282060512152448
1101	10-11	0.29921928931996433
1101	12-13	0.24007624074776146
1101	14-15	0.32069423246664996
1101	16-17	0.3099421078908762
1101	18-19	0.28640566047491234
1101	20-21	0.3077426882399763
1101	22-23	0.32656774455711
1101	24-25	0.32104500669547065
1101	26-27	0.2806189408800108
1101	28-29	0.29508067672318816
1101	30-31	0.3561012667103327
1101	32-33	0.3669059254444207
1101	34-35	0.29771442286431693
1101	36-37	0.2208427156474997
1101	38-39	0.22677972253944745
1101	40-41	0.22531133965181027
1101	42-43	0.2642142583175442
1101	44-45	0.25145644357634467
1101	46-47	0.22531830609385395
1101	48-49	0.2323436570420938
1101	50-51	0.1935002005891704
1101	52-53	0.2424421410309492
1101	54-55	0.21438669016821166
1101	56-57	0.2409529567847315
1101	58-59	0.2583418655660594
1101	60-61	0.24501177121732098
1101	62-63	0.24363317418065122
1101	64-65	0.30509602423045834
1101	66-67	0.21981747235159332
1101	68-69	0.2464141792277772
1101	70-71	0.18293395978562899
1101	72-73	0.2003662927687344
1101	74-75	0.15521909278474055
1101	76-77	0.10677269732472183
1101	78-79	0.07969999183494281
1101	80-81	0.07416604498717305
1101	82-83	0.17102791912706294
1101	84-85	0.14789819770671642
1101	86-87	0.12409686119724483
1101	88-89	0.15796870018219522
1101	90-91	0.15881234188273652
1101	92-93	0.19488463551233082
1101	94-95	0.18778915146408082
1101	96-97	0.21064695662229482
1101	98-99	0.22515683963441901
1101	100	0.23116190666655356
1102	1	0.1255306979455142
1102	2	0.14364967972540654
1102	3	0.19480489855334326
1102	4	0.12909992616494748
1102	5	0.14865401876663498
1102	6	0.18045355256575846
1102	7	0.19775393595541146
1102	8	0.20289417539962074
1102	9	0.2165191918428988
1102	10-11	0.24304943848763116
1102	12-13	0.22044717169690387
1102	14-15	0.2731264992617639
1102	16-17	0.34072662694546807
1102	18-19	0.3054067504052185
1102	20-21	0.30702380062473367
1102	22-23	0.31794048590306545
1102	24-25	0.34301079449500804
1102	26-27	0.3296488616165547
1102	28-29	0.3168339803391049
1102	30-31	0.3351008578953767
1102	32-33	0.4181361718240524
1102	34-35	0.33049176300200145
1102	36-37	0.27975166310810096
1102	38-39	0.2567570567501605
1102	40-41	0.2766696595219429
1102	42-43	0.30282182681105496
1102	44-45	0.2398155409964815
1102	46-47	0.23823065232975438
1102	48-49	0.2878371292413391
1102	50-51	0.26633800550305864
1102	52-53	0.24311056441167267
1102	54-55	0.2639884781688764
1102	56-57	0.2818722244545597
1102	58-59	0.23358068710047775
1102	60-61	0.16922876183886615
1102	62-63	0.2192194155534466
1102	64-65	0.27579148575290446
1102	66-67	0.2940919594078508
1102	68-69	0.25235553820835577
1102	70-71	0.2701595977057849
1102	72-73	0.25451326259489093
1102	74-75	0.2353654062722086
1102	76-77	0.18975132356558788
1102	78-79	0.15899685440486166
1102	80-81	0.18899495119219267
1102	82-83	0.22606126195811527
1102	84-85	0.18017964020408783
1102	86-87	0.23394656886905096
1102	88-89	0.2444602079806515
1102	90-91	0.2376890335276869
1102	92-93	0.2837384889029835
1102	94-95	0.2935468230600833
1102	96-97	0.26467799838141914
1102	98-99	0.26980293049627235
1102	100	0.30329466974796304
1103	1	0.17163813414589413
1103	2	0.1595865810977557
1103	3	0.15275154128865154
1103	4	0.13033104716296862
1103	5	0.1394987444826299
1103	6	0.1712286937016998
1103	7	0.16353960444921256
1103	8	0.16823794594171204
1103	9	0.23042128227367442
1103	10-11	0.23366353077591384
1103	12-13	0.26761212612856866
1103	14-15	0.35903278820133266
1103	16-17	0.38406102198167247
1103	18-19	0.3373849300199936
1103	20-21	0.3528334100400272
1103	22-23	0.36996888836785047
1103	24-25	0.3296853474140775
1103	26-27	0.311304716369861
1103	28-29	0.3664995558908615
1103	30-31	0.42828555043555383
1103	32-33	0.43459322391038313
1103	34-35	0.4397187500542543
1103	36-37	0.4355261311148553
1103	38-39	0.5087464344607611
1103	40-41	0.5777145520974543
1103	42-43	0.5180073581921363
1103	44-45	0.5106259335372059
1103	46-47	0.569134622978936
1103	48-49	0.496873810836405
1103	50-51	0.4954930000140081
1103	52-53	0.5402858608814327
1103	54-55	0.5482043639698162
1103	56-57	0.5179646939921767
1103	58-59	0.5119331753231009
1103	60-61	0.4724886439850593
1103	62-63	0.5107000749857065
1103	64-65	0.5312837057903508
1103	66-67	0.5078565664905099
1103	68-69	0.5524078940289883
1103	70-71	0.5107991565156311
1103	72-73	0.5414986625065623
1103	74-75	0.5447788010886612
1103	76-77	0.5336138679556441
1103	78-79	0.4942423289561546
1103	80-81	0.4614349022103852
1103	82-83	0.4792139097231285
1103	84-85	0.42907919952565976
1103	86-87	0.4432774306346232
1103	88-89	0.46696353835604043
1103	90-91	0.4766855234519056
1103	92-93	0.46850402114969825
1103	94-95	0.4522103663116894
1103	96-97	0.49906510768252943
1103	98-99	0.529413134895595
1103	100	0.5718189338432076
1104	1	0.13331923699257686
1104	2	0.17651722140085724
1104	3	0.1456565453355836
1104	4	0.06607920220627506
1104	5	0.10159583147595441
1104	6	0.07718571213262493
1104	7	0.11155224945490261
1104	8	0.13187157972261332
1104	9	0.235668327816839
1104	10-11	0.19176540224268024
1104	12-13	0.1770943464700352
1104	14-15	0.2626382559494189
1104	16-17	0.25174652952738086
1104	18-19	0.26299553145153
1104	20-21	0.22708473836664922
1104	22-23	0.2348462035519958
1104	24-25	0.2692742991944712
1104	26-27	0.2447649871182307
1104	28-29	0.24646543435643053
1104	30-31	0.2627665134157624
1104	32-33	0.24336967397294984
1104	34-35	0.3051006349055072
1104	36-37	0.33867860666815375
1104	38-39	0.30951235279694345
1104	40-41	0.2205483969097628
1104	42-43	0.3041732593985529
1104	44-45	0.3260413366175783
1104	46-47	0.3706884021735277
1104	48-49	0.3635769229224337
1104	50-51	0.27737794250555936
1104	52-53	0.24490133471616815
1104	54-55	0.24351328309189313
1104	56-57	0.2903593245520639
1104	58-59	0.24683737009697637
1104	60-61	0.2537776086413004
1104	62-63	0.22890654066237204
1104	64-65	0.2501099849943458
1104	66-67	0.2534094707213228
1104	68-69	0.2584756628930265
1104	70-71	0.23157457377424606
1104	72-73	0.23061534825507835
1104	74-75	0.25773594029469393
1104	76-77	0.24805677480154742
1104	78-79	0.23941588643774736
1104	80-81	0.271420557457553
1104	82-83	0.3053578262101624
1104	84-85	0.2725019398935018
1104	86-87	0.28072254439188526
1104	88-89	0.3069341520270328
1104	90-91	0.3043300336073429
1104	92-93	0.3687377078709204
1104	94-95	0.3066774144938904
1104	96-97	0.22718687910098012
1104	98-99	0.22843762783687183
1104	100	0.16228878551335058
1105	1	-0.03273313514575449
1105	2	0.007127367689477637
1105	3	0.03536566296446608
1105	4	-0.05996970962820569
1105	5	-0.07209656081124649
1105	6	-0.10802098001639138
1105	7	-0.4603553107018499
1105	8	-0.41436115034552046
1105	9	-0.3642341545290435
1105	10-11	-0.36549230463869975
1105	12-13	-0.16135607117487893
1105	14-15	-0.21022417806324256
1105	16-17	-0.21111314560999972
1105	18-19	-0.18761282338967789
1105	20-21	-0.1943527461034691
1105	22-23	-0.18852110579402392
1105	24-25	-0.16854408678079125
1105	26-27	-0.127608898713369
1105	28-29	-0.1346291226677252
1105	30-31	-0.16015961297126324
1105	32-33	-0.15019718515191016
1105	34-35	-0.16622154754468
1105	36-37	-0.1404967712344387
1105	38-39	-0.1435980434442783
1105	40-41	-0.22501484763165536
1105	42-43	-0.1751495457212826
1105	44-45	-0.16483442500056356
1105	46-47	-0.1589993163904353
1105	48-49	-0.23507836969153573
1105	50-51	-0.23500692678383928
1105	52-53	-0.21976641279429288
1105	54-55	-0.21465795210300342
1105	56-57	-0.24180562808646755
1105	58-59	-0.23693967993500564
1105	60-61	-0.35311955643653903
1105	62-63	-0.3800102529659526
1105	64-65	-0.2725018921401201
1105	66-67	-0.309563549028141
1105	68-69	-0.2831460850704488
1105	70-71	-0.2859125089241843
1105	72-73	-0.2569212837509127
1105	74-75	-0.2283739813690957
1105	76-77	-0.2301362272312133
1105	78-79	-0.3410799235240276
1105	80-81	-0.31694371400305243
1105	82-83	-0.2735769148531162
1105	84-85	-0.23500805864408392
1105	86-87	-0.24444943137974207
1105	88-89	-0.15728610949769006
1105	90-91	-0.1829162832999387
1105	92-93	-0.16939563283328596
1105	94-95	-0.18588724024857228
1105	96-97	-0.2016280090414
1105	98-99	-0.27153048116085543
1105	100	-0.24721174934762047
1106	1	0.08794986964935703
1106	2	0.0676341871960986
1106	3	0.06863520355498309
1106	4	0.01907405508664084
1106	5	0.09700070928649751
1106	6	0.09242863922935385
1106	7	0.07312927741449471
1106	8	0.06801896796416429
1106	9	0.06573432536958279
1106	10-11	0.09544123322969256
1106	12-13	0.10676576845443009
1106	14-15	0.1380702093370303
1106	16-17	0.16056122070327916
1106	18-19	0.121167176389541
1106	20-21	0.11989481266878954
1106	22-23	0.0980882733084627
1106	24-25	0.10517118012165128
1106	26-27	0.08237865190721294
1106	28-29	0.1065343396428986
1106	30-31	0.13165411647666048
1106	32-33	0.08342156686246938
1106	34-35	0.07930136210570282
1106	36-37	0.09760526713904483
1106	38-39	0.03121825163859171
1106	40-41	0.048664217259016596
1106	42-43	0.08945620733575765
1106	44-45	0.028352605306743328
1106	46-47	0.04312311718996398
1106	48-49	0.10157138899418783
1106	50-51	0.0929230984612559
1106	52-53	0.07032398725471722
1106	54-55	0.06823303665889568
1106	56-57	0.06455930788954589
1106	58-59	0.05752558983545697
1106	60-61	0.024020934858853593
1106	62-63	0.0663285710238668
1106	64-65	0.1145911761432572
1106	66-67	0.10908814952262702
1106	68-69	0.08459108247982527
1106	70-71	0.12940692702142087
1106	72-73	0.146483063607441
1106	74-75	0.20949846914886905
1106	76-77	0.17961635122866682
1106	78-79	0.13631787262303874
1106	80-81	0.07429180600394147
1106	82-83	0.10942795061068722
1106	84-85	0.11205121243679628
1106	86-87	0.1411766166657955
1106	88-89	0.168638221479398
1106	90-91	0.15524078321371348
1106	92-93	0.14997537642251757
1106	94-95	0.15487001276225953
1106	96-97	0.199040678372139
1106	98-99	0.1978344998376933
1106	100	0.21350711979027892
1107	1	-0.08758971651034386
1107	2	-0.015427827318930554
1107	3	-0.02565280684205362
1107	4	-0.12282055940676884
1107	5	-0.10784581142952732
1107	6	-0.1226460819356916
1107	7	-0.07379370831059617
1107	8	-0.038881897054501735
1107	9	-0.05103869277119344
1107	10-11	-0.057812379018642446
1107	12-13	-0.03677495525584362
1107	14-15	-0.01973341761841141
1107	16-17	0.029602868110629288
1107	18-19	-0.06677835174608049
1107	20-21	-0.02853668442580215
1107	22-23	-0.05079269132842512
1107	24-25	-0.07721303133669721
1107	26-27	-0.06437400137696869
1107	28-29	-0.08806315804597631
1107	30-31	-0.08666995862569138
1107	32-33	-0.10656251043094755
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2216	46-47	-0.6173045146487084
2216	48-49	-0.5616695644921634
2216	50-51	-0.4981791036532144
2216	52-53	-0.5247396127168642
2216	54-55	-0.5585798191416913
2216	56-57	-0.5972267419639934
2216	58-59	-0.5867965236891095
2216	60-61	-0.5968231958625765
2216	62-63	-0.7017056332066502
2216	64-65	-0.6480184360982548
2216	66-67	-0.538492524929211
2216	68-69	-0.6044682222236517
2216	70-71	-0.5439875980909008
2216	72-73	-0.6058899535815243
2216	74-75	-0.5209168848554384
2216	76-77	-0.4566123007361824
2216	78-79	-0.5005501742143323
2216	80-81	-0.4752148698217553
2216	82-83	-0.4575846667393577
2216	84-85	-0.5028675011935704
2216	86-87	-0.46161380741297364
2216	88-89	-0.44330080782160053
2216	90-91	-0.43215493415133466
2216	92-93	-0.6281906862220694
2216	94-95	-0.5965915627484009
2216	96-97	-0.6556442826625144
2216	98-99	-0.6648467265245195
2216	100	-0.6901992070448841
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	49.0
3	424.0
4	214.0
5	326.0
6	417.0
7	653.0
8	1129.0
9	1625.0
10	2397.0
11	3507.0
12	5946.0
13	9012.0
14	9178.0
15	12129.0
16	16773.0
17	27936.0
18	34601.0
19	17204.0
20	9677.0
21	8016.0
22	7795.0
23	7514.0
24	8412.0
25	9753.0
26	11910.0
27	14771.0
28	18250.0
29	22738.0
30	28528.0
31	35526.0
32	44614.0
33	58539.0
34	82210.0
35	143174.0
36	248493.0
37	570911.0
38	981326.0
39	501675.0
40	18287.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.852822812386776	21.834019747486526	14.888420449786683	22.424736990340012
2	14.059870837826766	22.095086131079743	40.7508101621198	23.094232868973688
3	15.316585697655348	29.76390834325842	32.2045056767925	22.715000282293733
4	10.406182638684912	18.792542440779176	40.07466834791995	30.72660657261596
5	11.5529135086615	38.626728578298646	35.59420346352498	14.226154449514878
6	27.0393686868602	40.49157172627459	18.586864871713267	13.882194715151938
7	23.889053746102938	34.92567478783549	24.516280368687195	16.66899109737438
8	24.934442652485735	34.4549187586263	21.506204213615966	19.104434375272
9	25.98440200575406	15.246372291800181	22.772117182225397	35.99710852022036
10-11	23.32740967570327	27.40807940748189	29.03203311960893	20.23247779720591
12-13	24.448026121448198	26.572090902155804	29.481129935452522	19.498753040943477
14-15	22.088986600861194	26.24096874654486	29.01645663334833	22.653588019245614
16-17	19.86726212420257	29.89962828152205	28.984984401669696	21.248125192605688
18-19	19.482941311093178	27.380455088806134	33.526950009729	19.609653590371682
20-21	21.558193046938825	26.30334190404145	33.07489584590066	19.06356920311906
22-23	20.57877710849042	26.76256437279291	33.420435160752184	19.238223357964483
24-25	20.20369440596525	26.68091909152806	33.037463612796465	20.077922889710226
26-27	19.94737566412641	27.153959032732512	33.13037839683467	19.7682869063064
28-29	19.950907404771776	26.65336739479396	33.65193558344422	19.743789616990043
30-31	20.608315822815122	26.857373783540588	32.93078605353388	19.603524340110415
32-33	19.544640401086667	26.936819204329737	33.70582888718648	19.81271150739712
34-35	20.39970606487376	26.4994480734495	33.00302320309637	20.09782265858037
36-37	20.8096904070549	26.85165954186159	32.43463327418401	19.9040167768995
38-39	20.218950566406463	26.749558696143495	32.79279597184729	20.238694765602748
40-41	20.421190563129436	26.91580675315737	32.49630361398085	20.16669906973235
42-43	20.15677405044999	27.094874345514548	32.711619763276225	20.036731840759238
44-45	20.384000580719214	26.692063050378906	32.022228641051505	20.901707727850376
46-47	21.01733597800202	27.26902894460378	31.333879563616225	20.379755513777976
48-49	20.893123507538615	27.118011802406894	31.007976765534245	20.98088792452025
50-51	21.358588202557392	27.55714708676638	29.868806776154543	21.215457934521684
52-53	20.791420059929994	27.90434725185872	30.403457946924668	20.900774741286618
54-55	21.086618229808078	27.743082003067254	30.123353855062117	21.046945912062554
56-57	21.25433291835	27.83591595906939	30.009495495310812	20.900255627269797
58-59	21.11616980984651	27.05761567537549	30.384348149000758	21.441866365777244
60-61	21.612081063787457	27.424190894572988	29.997207315958423	20.966520725681132
62-63	20.605758964713125	27.495875675779217	30.834385488293435	21.063979871214215
64-65	20.970537756764177	27.149849158449662	30.48918232352782	21.39043076125834
66-67	21.19457758696739	27.355510840205167	30.190876668248972	21.25903490457847
68-69	21.276456313371398	27.487563066836984	30.033088980321033	21.20289163947059
70-71	21.716111146865153	27.326250896254894	29.870715809188574	21.086922147691382
72-73	21.058177498248273	27.396589299426843	30.3227042295174	21.222528972807485
74-75	21.151391559701597	27.079811613324722	30.177743379278404	21.591053447695273
76-77	21.258197000905017	27.73184914392947	29.84924878118288	21.160705073982626
78-79	21.031519587580362	27.654497733237893	30.013593851344766	21.30038882783698
80-81	21.233473573344384	27.795600435943786	29.692184433930084	21.278741556781746
82-83	20.669103029445555	27.838220089625302	30.375497733994415	21.117179146934724
84-85	21.02547307915145	27.145323382215153	29.852850438834093	21.9763530997993
86-87	21.131486981089402	27.746734896406917	29.74192381923443	21.379854303269255
88-89	20.63424396506239	27.407304097674746	30.111003196004827	21.847448741258034
90-91	20.935795661055614	27.465401347062617	29.6575581511605	21.941244840721264
92-93	20.545165248920664	27.677360520628664	30.37744368154327	21.400030548907406
94-95	20.90738628811687	26.846067929458968	30.081800267945823	22.164745514478334
96-97	20.777947642353254	27.736036050871547	29.708042787053113	21.77797351972208
98-99	20.09627048490972	27.3138010827825	30.22725527456967	22.36267315773811
100	20.605658743278944	27.28791475286646	29.666779475435863	22.439647028418737
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1681.0
1	1144.0
2	2529.5
3	3663.0
4	1814.5
5	1035.0
6	2432.0
7	6337.0
8	10923.5
9	11482.0
10	7158.0
11	3447.5
12	2818.5
13	3006.5
14	3313.0
15	3624.5
16	4094.0
17	4687.5
18	5268.5
19	5852.0
20	6647.0
21	7758.5
22	9445.0
23	11967.0
24	15375.0
25	19442.0
26	24626.5
27	31167.0
28	39196.5
29	47573.0
30	54083.5
31	60416.0
32	67204.0
33	72164.0
34	77291.0
35	84968.5
36	91853.0
37	97247.0
38	105393.0
39	112515.5
40	117841.0
41	121674.5
42	120576.0
43	119479.0
44	117058.5
45	109670.5
46	102645.5
47	97720.0
48	88871.5
49	84968.5
50	86084.5
51	81494.5
52	73487.5
53	66972.0
54	62276.0
55	58050.5
56	52704.0
57	45902.5
58	42102.0
59	39133.0
60	34324.5
61	28455.0
62	23576.5
63	19123.5
64	14605.5
65	11461.5
66	9074.0
67	6772.5
68	4934.0
69	3524.0
70	2471.0
71	1881.5
72	1420.0
73	987.5
74	777.5
75	611.5
76	390.5
77	267.0
78	189.0
79	109.0
80	58.5
81	23.5
82	9.0
83	8.0
84	8.0
85	7.5
86	4.5
87	2.5
88	3.5
89	2.5
90	3.0
91	2.5
92	1.5
93	3.5
94	6.0
95	6.5
96	5.5
97	6.5
98	4.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009174499998151658
2	0.0
3	5.040934064918493E-4
4	0.002722104395055986
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.0081868129836988E-4
24-25	1.6803113549728312E-4
26-27	6.553214284394041E-4
28-29	0.0012602335162296232
30-31	0.0015626895601247328
32-33	0.004116762819683436
34-35	0.005124949632667135
36-37	0.003999141024835338
38-39	0.0027389075086057145
40-41	0.00327660714219702
42-43	6.385183148896758E-4
44-45	6.217152013399475E-4
46-47	0.0031253791202494655
48-49	0.001999570512417669
50-51	9.409743587847853E-4
52-53	1.680311354972831E-5
54-55	4.2007783874320774E-4
56-57	5.881089742404909E-4
58-59	5.545027471410343E-4
60-61	2.3524358969619633E-4
62-63	0.0
64-65	0.0
66-67	8.905650181356005E-4
68-69	8.73761904585872E-4
70-71	0.002100389193716039
72-73	1.3442490839782648E-4
74-75	0.0019155549446690273
76-77	2.6884981679565296E-4
78-79	9.74580585884242E-4
80-81	8.73761904585872E-4
82-83	9.577774723345137E-4
84-85	9.073681316853287E-4
86-87	2.0163736259673975E-4
88-89	0.0016467051278733745
90-91	0.0017979331498209294
92-93	0.002772513735705171
94-95	0.0012602335162296232
96-97	0.002218010988564137
98-99	0.002167601647914952
100	0.0034278351641445753
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2975639.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.1017334591234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.49697553235902	38.81421562697807
2	15.411558633919316	16.059378402682327
3	4.780221829700286	7.471735309395826
4	1.9763379246597572	4.1188252710311914
5	0.97733407593315	2.5460399762393826
6	0.5636017747242814	1.7618777666264054
7	0.34603686604545464	1.2620384393211468
8	0.2317179629277964	0.9658326033724067
9	0.16216373802661277	0.7604110669857923
>10	0.8853628466774939	8.615995326614435
>50	0.0883228669799747	3.203578985120607
>100	0.0693004492441793	7.133688670711708
>500	0.007765262317794866	2.7723418769585857
>1k	0.003041394407663609	2.4750066259496455
>5k	6.471051931199168E-5	0.18331009856132385
>10k+	1.9413155793597504E-4	1.8557239534511363
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24017	0.8071207562476497	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18233	0.6127423387043925	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12791	0.4298572508291496	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5437	0.18271705673974564	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3725	0.12518319594547592	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3651	0.12269633514011612	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	3.360622709945662E-5	0.0
18-19	0.0	0.0	0.0	1.3442490839782648E-4	0.0
20-21	0.0	0.0	0.0	1.848342490470114E-4	0.0
22-23	0.0	0.0	0.0	5.713058606907626E-4	0.0
24-25	0.0	0.0	0.0	0.0010417930400831552	0.0
26-27	0.0	0.0	0.0	0.0019323580582187557	0.0
28-29	0.0	0.0	0.0	0.0036630787538407717	0.0
30-31	0.0	0.0	0.0	0.008334344320665241	0.0
32-33	0.0	0.0	0.0	0.01712237270717315	0.0
34-35	0.0	0.0	0.0	0.03184190017673515	0.0
36-37	0.0	0.0	0.0	0.05266095786484852	0.0
38-39	0.0	0.0	0.0	0.08050371701674834	0.0
40-41	0.0	0.0	0.0	0.10832967305509841	0.0
42-43	0.0	0.0	0.0	0.13605481041215012	0.0
44-45	0.0	0.0	0.0	0.17155978934272603	0.0
46-47	0.0	0.0	0.0	0.21578558420561095	0.0
48-49	0.0	0.0	0.0	0.26076751917823365	0.0
50-51	0.0	0.0	0.0	0.3092276986556501	0.0
52-53	0.0	0.0	0.0	0.35809115285826	0.0
54-55	0.0	0.0	0.0	0.41070170138245937	0.0
56-57	0.0	0.0	0.0	0.47359575539909243	0.0
58-59	0.0	0.0	0.0	0.5484368231495823	0.0
60-61	0.0	0.0	0.0	0.6295790584812203	0.0
62-63	0.0	0.0	0.0	0.7090577855714353	0.0
64-65	0.0	0.0	0.0	0.7912586170567062	0.0
66-67	0.0	0.0	0.0	0.8912371426775896	0.0
68-69	0.0	0.0	0.0	1.0077667351449555	0.0
70-71	0.0	0.0	0.0	1.1420236124072847	0.0
72-73	0.0	0.0	0.0	1.2862951453452518	0.0
74-75	0.0	0.0	0.0	1.4380272606992985	0.0
76-77	0.0	0.0	0.0	1.5935736828291334	0.0
78-79	0.0	0.0	0.0	1.7726276608150384	0.0
80-81	0.0	0.0	0.0	1.9808686470368213	0.0
82-83	0.0	0.0	0.0	2.2021488493731933	0.0
84-85	0.0	0.0	0.0	2.427243358485354	0.0
86-87	0.0	0.0	0.0	2.6727200443333348	0.0
88	0.0	0.0	0.0	2.8694004884329045	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8670	0.0	63.218266	1
GTATCAA	17705	0.0	47.153095	1
ATCAACG	23490	0.0	35.395626	3
TCAACGC	23550	0.0	35.2843	4
GTGGTAT	2595	0.0	35.142086	1
CAACGCA	24215	0.0	34.31416	5
TATCAAC	25015	0.0	33.275364	2
AACGCAG	25140	0.0	33.163773	6
TGGTATC	2875	0.0	31.224846	2
ACGCAGA	28120	0.0	29.582407	7
CGCAGAG	28230	0.0	29.400547	8
GCAGAGT	29855	0.0	27.627121	9
TATAACG	260	1.8189894E-12	25.308096	2
TACATGG	17220	0.0	23.909788	2
GTACATG	18515	0.0	23.636826	1
GAGTACT	19635	0.0	23.253454	12-13
ACATGGG	17485	0.0	22.848518	3
AGAGTAC	29105	0.0	22.574345	10-11
GTATCGA	125	2.4222233E-4	22.563393	1
CAGAGTA	29770	0.0	22.520006	10-11
>>END_MODULE