FastQCFastQC Report
Wed 25 May 2016
SRR2431355_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431355_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3534835
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA187180.5295296668727112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42450.044.952241
GTATCAA75100.035.610281
ATCAACG81250.031.8731583
TCAACGC81450.031.737194
CAACGCA86900.029.584515
TATCAAC94350.028.1451422
AACGCAG91450.028.009786
ACGCAGA101500.024.8659557
CGCAGAG102700.024.4381168
GTACATG150150.024.1967581
TACATGG149850.023.5548342
ACATGGG155900.021.8267633
CATGGGT46850.020.9668674
GCAGAGT116050.020.6548429
TACCTGG24950.020.3446432
CGTCCGA1000.005665614318.7999576
GTACCTG28800.018.441191
CATGGGG75650.018.14144
CAGAGTA114700.017.4559310-11
AGAGTAC104450.017.18904710-11
Produced by FastQC (version 0.11.5)