##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431355_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3534835
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.805838462049856	33.0	31.0	34.0	27.0	34.0
2	30.921560695195108	33.0	31.0	34.0	27.0	34.0
3	30.953202058936274	34.0	31.0	34.0	27.0	34.0
4	34.197283041499816	37.0	35.0	37.0	30.0	37.0
5	34.32987169132364	37.0	35.0	37.0	31.0	37.0
6	34.336274536152324	37.0	35.0	37.0	31.0	37.0
7	34.2850183954838	37.0	35.0	37.0	30.0	37.0
8	34.24397631006822	37.0	35.0	37.0	30.0	37.0
9	35.67541398679146	39.0	37.0	39.0	30.0	39.0
10-11	35.71109005087932	39.0	37.0	39.0	30.0	39.0
12-13	35.65489619741798	39.0	37.0	39.0	30.0	39.0
14-15	36.96988869353166	40.0	38.0	41.0	30.0	41.0
16-17	36.95410860761535	40.0	38.0	41.0	30.0	41.0
18-19	36.95789053803077	40.0	38.0	41.0	30.0	41.0
20-21	36.999161064094935	40.0	38.0	41.0	30.0	41.0
22-23	36.98698411665608	40.0	38.0	41.0	30.0	41.0
24-25	37.007762455673316	40.0	38.0	41.0	30.0	41.0
26-27	37.011825304434296	40.0	38.0	41.0	30.0	41.0
28-29	36.985318550936604	40.0	38.0	41.0	30.0	41.0
30-31	36.9188725923558	40.0	38.0	41.0	30.0	41.0
32-33	36.8466963804534	40.0	38.0	41.0	30.0	41.0
34-35	36.782911224993526	40.0	37.5	41.0	30.0	41.0
36-37	36.688942623913135	40.0	37.0	41.0	30.0	41.0
38-39	36.586143059011235	40.0	37.0	41.0	29.0	41.0
40-41	36.49821250496841	40.0	37.0	41.0	29.0	41.0
42-43	36.36607479557037	40.0	37.0	41.0	28.5	41.0
44-45	36.22263005203921	40.0	36.5	41.0	28.0	41.0
46-47	36.08572168149291	40.0	36.0	41.0	28.0	41.0
48-49	35.950593874961626	40.0	36.0	41.0	28.0	41.0
50-51	35.81457988845307	40.0	35.5	41.0	27.5	41.0
52-53	35.643165381128114	39.5	35.0	41.0	27.0	41.0
54-55	35.47492301620868	39.0	35.0	41.0	27.0	41.0
56-57	35.27657853902657	39.0	35.0	41.0	27.0	41.0
58-59	35.051326440979565	39.0	34.5	40.5	26.0	41.0
60-61	34.8814408593329	39.0	34.0	40.0	26.0	41.0
62-63	34.727233661542904	38.0	34.0	40.0	26.0	41.0
64-65	34.47801608844543	38.0	34.0	40.0	26.0	41.0
66-67	34.18107478849791	37.5	34.0	40.0	25.5	41.0
68-69	33.87951276367921	37.0	34.0	40.0	25.0	41.0
70-71	33.63384259802791	36.5	33.0	39.5	25.0	41.0
72-73	33.39899839737923	36.0	33.0	39.0	24.5	41.0
74-75	33.056583263433794	36.0	33.0	39.0	24.0	40.5
76-77	32.701709980805326	35.0	33.0	38.5	23.0	40.0
78-79	32.352780257069995	35.0	33.0	37.0	22.5	39.0
80-81	32.00166245383448	35.0	32.5	37.0	22.0	39.0
82-83	31.6577257495753	35.0	32.0	36.5	20.0	39.0
84-85	31.355737962309416	35.0	32.0	36.0	20.0	37.0
86-87	31.076128447296693	35.0	32.0	36.0	19.0	37.0
88-89	30.815816155492406	35.0	32.0	35.0	18.0	37.0
90-91	30.59229214376343	35.0	31.5	35.0	16.0	36.0
92-93	30.374471509985614	35.0	31.0	35.0	8.5	36.0
94-95	30.14460165750311	35.0	31.0	35.0	3.5	36.0
96-97	29.940916761319833	34.0	31.0	35.0	2.0	35.5
98-99	29.806576968939144	34.5	31.0	35.0	2.0	35.0
100	29.754629848352184	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.60585263363512
1101	2	0.6010158336221529
1101	3	0.5607275853636402
1101	4	0.6348950356413567
1101	5	0.5479570060944852
1101	6	0.5735261247457402
1101	7	0.6264381662974685
1101	8	0.6059816057015084
1101	9	0.7562150137744439
1101	10-11	0.7002157770118487
1101	12-13	0.7131493238268618
1101	14-15	0.8278244728546795
1101	16-17	0.8058130636519039
1101	18-19	0.7752201880552576
1101	20-21	0.7539621394398353
1101	22-23	0.7819645797054378
1101	24-25	0.7748375542081831
1101	26-27	0.7878160794250704
1101	28-29	0.8951223742854921
1101	30-31	0.8847953117267977
1101	32-33	0.9261890103367278
1101	34-35	0.9666476327652802
1101	36-37	1.0181811414788626
1101	38-39	0.9357969594434934
1101	40-41	0.9044314838828527
1101	42-43	0.9227659737342222
1101	44-45	0.9671879977777067
1101	46-47	1.0233533956221024
1101	48-49	1.0005431672118732
1101	50-51	0.964268766246704
1101	52-53	0.9820237256960027
1101	54-55	1.057832589587285
1101	56-57	0.9821137988099196
1101	58-59	1.020118707074083
1101	60-61	1.0257930397822363
1101	62-63	1.0382660270811854
1101	64-65	1.043299442667724
1101	66-67	0.9910296458578074
1101	68-69	1.0358948198779387
1101	70-71	0.987963652579289
1101	72-73	1.0691244193263643
1101	74-75	1.0229996132796373
1101	76-77	1.0323188277633975
1101	78-79	0.9348633460754598
1101	80-81	0.8835960272710466
1101	82-83	0.9333310642881436
1101	84-85	0.953074173845522
1101	86-87	0.9764805884857886
1101	88-89	1.0021898221757972
1101	90-91	0.9987037028467682
1101	92-93	1.0026754222861314
1101	94-95	1.0590047057459628
1101	96-97	1.0335406123213673
1101	98-99	1.1003870910643805
1101	100	1.1193063559824772
1102	1	0.267826985852075
1102	2	0.4017528160202737
1102	3	0.4227878919978352
1102	4	0.38421839965685933
1102	5	0.37130644723645645
1102	6	0.35871681135401445
1102	7	0.329283303803102
1102	8	0.38285925019726363
1102	9	0.48237346516150126
1102	10-11	0.46048709839683966
1102	12-13	0.39489452084730914
1102	14-15	0.5158547758590046
1102	16-17	0.5772914230456152
1102	18-19	0.5829250422250922
1102	20-21	0.4920300700062228
1102	22-23	0.47575715504757454
1102	24-25	0.5244942423551677
1102	26-27	0.5077317761968203
1102	28-29	0.4986343486634297
1102	30-31	0.5516426004076109
1102	32-33	0.6042868886360395
1102	34-35	0.5807060409608056
1102	36-37	0.6456030782189401
1102	38-39	0.573270487694856
1102	40-41	0.6156672505223497
1102	42-43	0.6580178383518174
1102	44-45	0.5352004377634998
1102	46-47	0.5209791899031586
1102	48-49	0.4699747809824828
1102	50-51	0.5036640264255681
1102	52-53	0.4788182243389585
1102	54-55	0.44867669522840004
1102	56-57	0.4951233419278154
1102	58-59	0.5164125765334404
1102	60-61	0.5219440805371107
1102	62-63	0.5195505800967908
1102	64-65	0.5150374485286662
1102	66-67	0.4809567071992191
1102	68-69	0.5187132811652688
1102	70-71	0.5525745232325576
1102	72-73	0.5545708516179246
1102	74-75	0.5371586445546654
1102	76-77	0.5581989432128935
1102	78-79	0.5105600732405762
1102	80-81	0.5847334787234288
1102	82-83	0.6092695800458507
1102	84-85	0.6076384940006072
1102	86-87	0.5983553532599437
1102	88-89	0.5410032654260775
1102	90-91	0.5346404251011769
1102	92-93	0.5860063694256255
1102	94-95	0.6130222903085283
1102	96-97	0.5854208673417602
1102	98-99	0.6573870178496684
1102	100	0.7104849945694411
1103	1	0.40372538986686024
1103	2	0.39095796644575387
1103	3	0.477927890906642
1103	4	0.5749933768204869
1103	5	0.4881198725110707
1103	6	0.41142162822959705
1103	7	0.5062696829842324
1103	8	0.5343726596395513
1103	9	0.6032454713712525
1103	10-11	0.6400058543444231
1103	12-13	0.62372054570573
1103	14-15	0.6204351791246765
1103	16-17	0.5666157761378869
1103	18-19	0.6180150186908264
1103	20-21	0.5150371888614487
1103	22-23	0.5225491598247203
1103	24-25	0.5168329710631241
1103	26-27	0.5301553500910927
1103	28-29	0.5057585594321523
1103	30-31	0.4779810844342052
1103	32-33	0.4212933895955189
1103	34-35	0.5631208652370745
1103	36-37	0.5948135373262033
1103	38-39	0.5169013080401612
1103	40-41	0.4931538959743733
1103	42-43	0.48732271567808283
1103	44-45	0.4825305155962738
1103	46-47	0.4687188830774218
1103	48-49	0.47841181101268404
1103	50-51	0.38839508947737755
1103	52-53	0.42091137630684017
1103	54-55	0.37654586032271453
1103	56-57	0.3094925309547065
1103	58-59	0.30372405724394724
1103	60-61	0.3415501954289013
1103	62-63	0.2689532697173078
1103	64-65	0.3866249217802462
1103	66-67	0.3480603359476433
1103	68-69	0.3579568325060123
1103	70-71	0.40999265288288456
1103	72-73	0.3644208411913539
1103	74-75	0.4440606086665042
1103	76-77	0.4382075053868917
1103	78-79	0.3279056470016286
1103	80-81	0.41025880726088104
1103	82-83	0.44866187321897044
1103	84-85	0.3910316838924217
1103	86-87	0.4583997071957171
1103	88-89	0.46091355004305257
1103	90-91	0.4741613266130784
1103	92-93	0.4946472950106937
1103	94-95	0.49026843841807377
1103	96-97	0.5585468374062827
1103	98-99	0.5001941808211754
1103	100	0.3882673412946467
1104	1	0.23483625318971946
1104	2	0.29062397647355454
1104	3	0.21421686156565656
1104	4	0.18239147628460017
1104	5	0.32389344360676375
1104	6	0.28515183256913446
1104	7	0.31154123508218845
1104	8	0.3057877592837883
1104	9	0.3599743519497096
1104	10-11	0.35693243659153495
1104	12-13	0.40508815577851465
1104	14-15	0.4598070406720396
1104	16-17	0.40864897881056805
1104	18-19	0.47110624860687267
1104	20-21	0.3938817188881387
1104	22-23	0.4089395568863381
1104	24-25	0.4597682897521693
1104	26-27	0.4613481264917425
1104	28-29	0.5207390270467016
1104	30-31	0.5013264307325827
1104	32-33	0.49773594608409155
1104	34-35	0.5294921873684757
1104	36-37	0.5782827253336293
1104	38-39	0.5507563309462782
1104	40-41	0.5054974439018665
1104	42-43	0.4784781046436919
1104	44-45	0.4897826105982901
1104	46-47	0.49394205928441437
1104	48-49	0.4555639472809787
1104	50-51	0.46102230253619325
1104	52-53	0.43306241182347094
1104	54-55	0.41446520619984994
1104	56-57	0.5082039367432358
1104	58-59	0.426097620905999
1104	60-61	0.5223387672441291
1104	62-63	0.5254443688774018
1104	64-65	0.5500829688466666
1104	66-67	0.5308508700552181
1104	68-69	0.5897259310237644
1104	70-71	0.542179584339884
1104	72-73	0.44412586357329786
1104	74-75	0.5880302288738193
1104	76-77	0.543318090424485
1104	78-79	0.6486638456954026
1104	80-81	0.6156265440868793
1104	82-83	0.593514119943638
1104	84-85	0.5967865898615443
1104	86-87	0.5599283224501299
1104	88-89	0.6142899508805755
1104	90-91	0.5454722837142647
1104	92-93	0.5390539279209747
1104	94-95	0.5306961638267573
1104	96-97	0.4877380089014878
1104	98-99	0.5722261064313336
1104	100	0.5834703990016692
1105	1	0.27988181372864673
1105	2	0.3487660556285128
1105	3	0.31063919920034877
1105	4	0.3363504653360607
1105	5	0.35187052585904866
1105	6	0.2953211390006629
1105	7	0.3413123946587149
1105	8	0.37610608151457114
1105	9	0.40052865221156964
1105	10-11	0.4030772680079906
1105	12-13	0.4638367927928684
1105	14-15	0.45523738567147376
1105	16-17	0.47623607566560366
1105	18-19	0.5406250993061263
1105	20-21	0.4378217829695785
1105	22-23	0.4387577850196607
1105	24-25	0.41551341752208515
1105	26-27	0.4652522590634476
1105	28-29	0.46512249120841886
1105	30-31	0.46592411511819876
1105	32-33	0.5385965686722258
1105	34-35	0.5966896501551702
1105	36-37	0.6009302947760062
1105	38-39	0.5879538341794159
1105	40-41	0.5705459911330379
1105	42-43	0.5425480462192027
1105	44-45	0.49908675968165284
1105	46-47	0.4596659391895628
1105	48-49	0.39200462222267163
1105	50-51	0.39068782155486304
1105	52-53	0.49647672448732294
1105	54-55	0.4545431901143857
1105	56-57	0.3622643274066988
1105	58-59	0.4214114246346341
1105	60-61	0.42207134091395915
1105	62-63	0.517133953212479
1105	64-65	0.5426622757960686
1105	66-67	0.561567187665112
1105	68-69	0.4597235486489595
1105	70-71	0.49148179199398356
1105	72-73	0.4785992393849128
1105	74-75	0.5185094153324812
1105	76-77	0.5603538546762863
1105	78-79	0.5458694485270286
1105	80-81	0.4276523343251242
1105	82-83	0.5097145422203688
1105	84-85	0.5804490025933902
1105	86-87	0.5895990003729672
1105	88-89	0.5357855857973384
1105	90-91	0.5239123072303009
1105	92-93	0.559574093764077
1105	94-95	0.5971301603469357
1105	96-97	0.6349178172123118
1105	98-99	0.5903091758119707
1105	100	0.629356849022912
1106	1	0.3244344451010015
1106	2	0.36121367857617415
1106	3	0.31460407200108875
1106	4	0.25661261395498514
1106	5	0.297356619410472
1106	6	0.35539550706653955
1106	7	0.3392249622484158
1106	8	0.38193159826528245
1106	9	0.33719212329457804
1106	10-11	0.3657781977092256
1106	12-13	0.3657102531571894
1106	14-15	0.35607872302598054
1106	16-17	0.41093591505877924
1106	18-19	0.39685666087667926
1106	20-21	0.43268749451518573
1106	22-23	0.4133941192622501
1106	24-25	0.38950360000263373
1106	26-27	0.43119740298428866
1106	28-29	0.4508173097438757
1106	30-31	0.45813657329369306
1106	32-33	0.4350232902754243
1106	34-35	0.4355693567870773
1106	36-37	0.43990274697050324
1106	38-39	0.43839108021312256
1106	40-41	0.44711110445150126
1106	42-43	0.44095139849660825
1106	44-45	0.4355718781266873
1106	46-47	0.452417033651102
1106	48-49	0.4156652019445062
1106	50-51	0.41007463828434254
1106	52-53	0.429649676156842
1106	54-55	0.4754319007338026
1106	56-57	0.5139433963580942
1106	58-59	0.5048730286107102
1106	60-61	0.45847364560467696
1106	62-63	0.5261882205033643
1106	64-65	0.5915916944146673
1106	66-67	0.5835192772792368
1106	68-69	0.584083900943341
1106	70-71	0.6229795908387175
1106	72-73	0.6249211019056915
1106	74-75	0.6199778623974908
1106	76-77	0.6377087840938103
1106	78-79	0.6981808304340902
1106	80-81	0.630657217525048
1106	82-83	0.5736132589540581
1106	84-85	0.6138759456540015
1106	86-87	0.632904368241487
1106	88-89	0.611991729971777
1106	90-91	0.6116189376799497
1106	92-93	0.6497376052863473
1106	94-95	0.6483464325924047
1106	96-97	0.6208375254800487
1106	98-99	0.5668124891837074
1106	100	0.5606306344713907
1107	1	-0.061292442395497915
1107	2	0.03676790468269786
1107	3	0.058504502048947415
1107	4	-0.009017114580515795
1107	5	-0.04677781293406014
1107	6	0.0026534060489709077
1107	7	-0.05130175004340032
1107	8	-0.06425326836593825
1107	9	-0.05091521654856024
1107	10-11	-0.012572415250275526
1107	12-13	-0.0031731573740998442
1107	14-15	0.02141348407196375
1107	16-17	0.026524379294663447
1107	18-19	-0.025274747774119533
1107	20-21	-0.05482576786282323
1107	22-23	-0.001003203599907465
1107	24-25	0.03556984381459216
1107	26-27	0.038548340920215196
1107	28-29	0.06964861807585976
1107	30-31	0.0724491839205541
1107	32-33	0.023310956713451958
1107	34-35	0.06023676974349712
1107	36-37	0.08133406262409437
1107	38-39	0.004399878339825136
1107	40-41	-0.02713615123173696
1107	42-43	-0.025843524677931384
1107	44-45	-0.006771426716618123
1107	46-47	-0.011715667840711319
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2216	94-95	-1.5900667395448416
2216	96-97	-1.7406471488314992
2216	98-99	-1.8387948643722467
2216	100	-1.7652445366697869
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81581.0
3	15748.0
4	11199.0
5	10150.0
6	7436.0
7	6592.0
8	7065.0
9	7569.0
10	7920.0
11	8415.0
12	9534.0
13	10200.0
14	10496.0
15	10523.0
16	10551.0
17	10586.0
18	10399.0
19	10164.0
20	9862.0
21	10544.0
22	12738.0
23	20418.0
24	32479.0
25	39806.0
26	37356.0
27	30059.0
28	31981.0
29	38082.0
30	45190.0
31	54651.0
32	71407.0
33	95540.0
34	134356.0
35	213294.0
36	363863.0
37	671814.0
38	971728.0
39	402676.0
40	863.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53583753886683	21.77679416842748	14.15036718911562	22.537001103590075
2	14.992678577086549	22.506416146509935	40.290316484630665	22.21058879177285
3	17.061310375536728	29.115511506339477	30.494840776115655	23.32833734200814
4	11.993466169406023	19.590939656838103	39.751467749678696	28.66412642407718
5	11.982175117700011	40.1676005153283	35.23356400894639	12.6166603580253
6	27.179514743969662	41.12831291983925	18.93225567812925	12.759916658061835
7	24.419131303158423	35.1468456094839	23.863207193546515	16.57081589381117
8	23.499371257781483	37.43393963226006	21.52719999660522	17.53948911335324
9	24.612803709366915	18.882493808056104	21.73046266657425	34.774239816002726
10-11	22.132320179018254	29.321425186748463	29.589613093680466	18.956641540552813
12-13	22.98581121891121	27.62717354558275	28.830935531644336	20.556079703861705
14-15	20.982888876001287	29.065515080619036	26.44105028947603	23.510545753903646
16-17	20.375986432181417	31.774651433518112	26.961017982451796	20.888344151848674
18-19	20.401758497921403	30.826941002903958	28.687958561007797	20.083341938166846
20-21	22.125346727640753	29.826795310106412	27.942930858158867	20.104927104093967
22-23	21.591955494386582	29.74959227234086	28.011434762867292	20.647017470405267
24-25	21.24162796849075	29.939671865872096	27.971560200122497	20.847139965514653
26-27	21.08508600825781	29.819185336797897	28.042652627350357	21.053076027593935
28-29	21.035239268593866	29.759918072555013	28.338182687452175	20.86665997139895
30-31	21.34064249109223	30.03933705533639	27.908784992793155	20.711235460778227
32-33	20.904540098759913	29.881097703287423	28.328649003418832	20.885713194533835
34-35	21.186208125697522	29.535636033930864	28.255392401625535	21.022763438746082
36-37	21.496731813507562	29.74151551628294	27.816178124297174	20.945574545912326
38-39	21.285434822276002	29.74715934407122	28.09363096155832	20.873774872094454
40-41	21.093686126792342	29.583573207801777	28.258419417030783	21.0643212483751
42-43	21.011009000420106	29.655075838051847	28.250073907268657	21.08384125425939
44-45	21.180422848591235	29.680211381860822	28.12278366599855	21.01658210354939
46-47	21.214497989297946	29.734174296678628	28.015225604589748	21.03610210943368
48-49	21.080022122673338	29.456212241872677	28.20418774850877	21.25957788694522
50-51	21.126841846932034	29.436706380920185	28.087152582793824	21.34929918935396
52-53	21.079116847038122	29.565467695097507	28.066868750592317	21.28854670727205
54-55	21.18523212540331	29.835126674936735	27.957882051071692	21.021759148588266
56-57	21.180352123932234	29.548663516118857	27.919337111916114	21.351647248032794
58-59	21.13726666166879	29.184756855694822	28.172389941821898	21.505586540814495
60-61	21.332975938056514	29.586501208684425	27.921671025663148	21.15885182759591
62-63	21.07506023266436	29.55387303139652	28.045584595261815	21.325482140677305
64-65	21.149522155778744	29.429585660882516	28.088193108926756	21.33269907441198
66-67	21.079116847038122	29.333660552755646	27.996837193249473	21.59038540695676
68-69	21.34205698427225	29.517459796567593	27.93325572480752	21.207227494352637
70-71	21.398806450654696	29.28962738006159	28.01931348988001	21.292252679403706
72-73	21.073359859795435	29.315342866074374	28.201132443239924	21.41016483089027
74-75	21.125483933479213	29.346985078511445	28.13984245374961	21.387688534259734
76-77	21.211782162392304	29.421118666076353	27.924570736682192	21.44252843484915
78-79	21.285689431048407	29.41031193818099	28.059470951260806	21.24452767950979
80-81	21.163788408794186	29.401358196351456	28.11382992416902	21.321023470685336
82-83	21.09050505964926	29.38593935210463	28.247666790199244	21.275888798046864
84-85	21.08195799796	29.25907217782483	28.095514193217756	21.563455630997414
86-87	21.334810276001654	29.352891743318075	27.949126671126162	21.363171309554104
88-89	21.078178905002222	29.317248511515555	28.08084205833023	21.523730525151997
90-91	21.02069589082408	29.238849696108804	28.058280456911135	21.682173956155985
92-93	21.13587488583015	29.386190926076782	28.031967133228875	21.44596705486419
94-95	21.115675468925645	29.074118422052102	28.173546939809558	21.636659169212695
96-97	21.057767046254234	29.235414310599804	28.10460123502107	21.602217408124886
98-99	20.859852204415198	29.2201393763118	28.156725219655044	21.763283199617963
100	20.904528129046458	29.27934745738497	28.140702238107778	21.675422175460792
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15245.0
1	10066.0
2	3846.0
3	2321.5
4	1572.5
5	1201.0
6	1072.0
7	1097.0
8	1211.5
9	1227.0
10	1060.0
11	921.0
12	898.0
13	965.5
14	1069.0
15	1191.5
16	1423.0
17	1773.5
18	2266.5
19	3029.0
20	4154.0
21	5652.5
22	7973.0
23	11533.0
24	15976.5
25	21538.5
26	29195.0
27	38944.0
28	50336.0
29	62313.0
30	72560.5
31	82026.5
32	90933.5
33	98506.5
34	105929.5
35	113201.5
36	119365.5
37	124081.0
38	128277.5
39	130277.0
40	131087.0
41	130798.0
42	128635.0
43	128145.5
44	125913.5
45	120671.0
46	117248.0
47	114160.0
48	109732.0
49	106244.0
50	103747.5
51	98315.5
52	90268.5
53	83026.5
54	77603.0
55	73226.5
56	66678.5
57	58452.5
58	52467.5
59	48037.5
60	42161.0
61	35508.5
62	29595.5
63	23937.5
64	18940.5
65	15000.0
66	11527.5
67	8730.5
68	6586.5
69	4877.5
70	3505.0
71	2572.5
72	2013.5
73	1511.0
74	1136.0
75	897.5
76	656.0
77	488.0
78	388.5
79	290.5
80	215.5
81	170.0
82	145.0
83	119.5
84	102.5
85	88.0
86	72.5
87	57.5
88	49.5
89	48.0
90	41.0
91	35.0
92	31.5
93	29.5
94	25.0
95	17.0
96	20.5
97	27.5
98	25.0
99	24.5
100	57.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.263189088033812E-4
2	8.486959080126795E-5
3	2.828986360042265E-5
4	5.65797272008453E-5
5	2.828986360042265E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	1.4144931800211325E-5
64-65	2.828986360042265E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.4144931800211326E-4
84-85	8.628408398128908E-4
86-87	0.0015700874298234572
88-89	0.003267479245848816
90-91	0.002814841428242054
92-93	0.0022490441562336007
94-95	0.0022490441562336007
96-97	0.0021924644290327554
98-99	0.0024895079968371933
100	0.0030553052688456457
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3534835.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.86928958765108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.91119139651578	51.97824117803659
2	13.61992502401748	17.942695711382058
3	3.772510390780051	7.454777382081418
4	1.45742841855717	3.839990982208583
5	0.6840410736856507	2.2528649786223953
6	0.39001681488916495	1.5414078314392625
7	0.24204371102538938	1.1160273106080774
8	0.16068722059521953	0.8467482453137029
9	0.12056934748965442	0.7147635538671161
>10	0.5739853805412843	6.7916127933039805
>50	0.04469086302509778	2.0521745776637053
>100	0.022350722040582274	2.693213879536131
>500	4.7353886446493835E-4	0.21074225411300543
>1k	4.304898645061467E-5	0.03397056173654545
>5k	0.0	0.0
>10k+	4.304898645061466E-5	0.5307687600873604
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18718	0.5295296668727112	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.828986360042265E-5	0.0
4	0.0	0.0	0.0	5.65797272008453E-5	0.0
5	0.0	0.0	0.0	5.65797272008453E-5	0.0
6	0.0	0.0	0.0	5.65797272008453E-5	0.0
7	0.0	0.0	0.0	5.65797272008453E-5	0.0
8	0.0	0.0	0.0	5.65797272008453E-5	0.0
9	0.0	0.0	0.0	8.486959080126795E-5	0.0
10-11	0.0	0.0	0.0	1.1315945440169061E-4	0.0
12-13	0.0	0.0	0.0	1.4144931800211326E-4	0.0
14-15	0.0	0.0	0.0	1.4144931800211326E-4	0.0
16-17	0.0	0.0	0.0	1.9802904520295855E-4	0.0
18-19	0.0	0.0	0.0	2.1217397700316987E-4	0.0
20-21	0.0	0.0	0.0	2.5460877240380387E-4	0.0
22-23	0.0	0.0	0.0	3.253334314048605E-4	0.0
24-25	0.0	0.0	0.0	4.950726130073964E-4	1.4144931800211325E-5
26-27	0.0	0.0	0.0	9.335654988139475E-4	2.828986360042265E-5
28-29	0.0	1.4144931800211325E-5	0.0	0.00213588470183191	2.828986360042265E-5
30-31	0.0	2.828986360042265E-5	0.0	0.005587248061083473	2.828986360042265E-5
32-33	0.0	2.828986360042265E-5	0.0	0.012362670393384699	2.828986360042265E-5
34-35	0.0	2.828986360042265E-5	0.0	0.023862499946956505	2.828986360042265E-5
36-37	0.0	2.828986360042265E-5	0.0	0.0407232586528084	2.828986360042265E-5
38-39	0.0	2.828986360042265E-5	0.0	0.06276106239753765	2.828986360042265E-5
40-41	0.0	2.828986360042265E-5	0.0	0.08665185220809457	2.828986360042265E-5
42-43	0.0	2.828986360042265E-5	0.0	0.11076896092745489	2.828986360042265E-5
44-45	0.0	2.828986360042265E-5	0.0	0.1405440423668997	2.828986360042265E-5
46-47	0.0	2.828986360042265E-5	0.0	0.17791495218305806	2.828986360042265E-5
48-49	0.0	2.828986360042265E-5	0.0	0.21753490615544996	2.828986360042265E-5
50-51	0.0	2.828986360042265E-5	0.0	0.25721143985504274	2.828986360042265E-5
52-53	0.0	2.828986360042265E-5	0.0	0.29705771273623804	2.828986360042265E-5
54-55	0.0	2.828986360042265E-5	0.0	0.3419820161337092	2.828986360042265E-5
56-57	0.0	2.828986360042265E-5	0.0	0.39663803260972574	2.828986360042265E-5
58-59	0.0	2.828986360042265E-5	0.0	0.4604033851650784	2.828986360042265E-5
60-61	0.0	2.828986360042265E-5	0.0	0.5285678115102967	2.828986360042265E-5
62-63	0.0	2.828986360042265E-5	0.0	0.5993631951703544	2.828986360042265E-5
64-65	0.0	2.828986360042265E-5	0.0	0.6759297110048984	2.828986360042265E-5
66-67	0.0	2.828986360042265E-5	0.0	0.7645052739378217	2.828986360042265E-5
68-69	0.0	2.828986360042265E-5	0.0	0.869743566531394	2.828986360042265E-5
70-71	0.0	2.828986360042265E-5	0.0	0.9870757758141468	2.828986360042265E-5
72-73	0.0	2.828986360042265E-5	0.0	1.1093304213633735	2.828986360042265E-5
74-75	0.0	2.828986360042265E-5	0.0	1.2396052432433198	2.828986360042265E-5
76-77	0.0	2.828986360042265E-5	0.0	1.379116705588804	2.828986360042265E-5
78-79	0.0	2.828986360042265E-5	0.0	1.535206028004136	2.828986360042265E-5
80-81	0.0	2.828986360042265E-5	0.0	1.7220605770849275	2.828986360042265E-5
82-83	0.0	2.828986360042265E-5	0.0	1.9203866658556907	2.828986360042265E-5
84-85	0.0	4.2434795400633975E-5	0.0	2.1226733355305125	2.828986360042265E-5
86-87	0.0	5.65797272008453E-5	0.0	2.3373792553259207	2.828986360042265E-5
88	0.0	5.65797272008453E-5	0.0	2.5109234235827134	2.828986360042265E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4245	0.0	44.95224	1
GTATCAA	7510	0.0	35.61028	1
ATCAACG	8125	0.0	31.873158	3
TCAACGC	8145	0.0	31.73719	4
CAACGCA	8690	0.0	29.58451	5
TATCAAC	9435	0.0	28.145142	2
AACGCAG	9145	0.0	28.00978	6
ACGCAGA	10150	0.0	24.865955	7
CGCAGAG	10270	0.0	24.438116	8
GTACATG	15015	0.0	24.196758	1
TACATGG	14985	0.0	23.554834	2
ACATGGG	15590	0.0	21.826763	3
CATGGGT	4685	0.0	20.966867	4
GCAGAGT	11605	0.0	20.654842	9
TACCTGG	2495	0.0	20.344643	2
CGTCCGA	100	0.0056656143	18.799957	6
GTACCTG	2880	0.0	18.44119	1
CATGGGG	7565	0.0	18.1414	4
CAGAGTA	11470	0.0	17.45593	10-11
AGAGTAC	10445	0.0	17.189047	10-11
>>END_MODULE