FastQCFastQC Report
Wed 25 May 2016
SRR2431355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431355_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3534835
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177100.5010134843634851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136500.38615663814576917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96540.27311034319848027No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42840.12119377566421063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77100.057.1847761
GTATCAA153400.044.8894461
ATCAACG201550.033.8360563
TCAACGC203300.033.5679134
TATCAAC208300.032.8298452
CAACGCA209350.032.619365
AACGCAG216950.031.4550066
ACGCAGA242300.028.1641087
CGCAGAG243050.028.0385288
GCAGAGT260950.025.8450539
TAGACCG1101.019162E-425.6354775
GTGGTAT31150.024.1431481
GTACATG148350.022.6859761
TGGTATC32850.022.3194922
AGAGTAC251150.022.23136310-11
TACATGG146200.022.1496542
GAGTACT172750.021.76480312-13
AGTACTT180050.021.60019712-13
ACATGGG149650.021.0108933
CAGAGTA259950.020.81886110-11
Produced by FastQC (version 0.11.5)