##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431355_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3534835
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77314895886229	34.0	31.0	34.0	31.0	34.0
2	32.89427936523204	34.0	31.0	34.0	31.0	34.0
3	32.96792353815666	34.0	31.0	34.0	31.0	34.0
4	36.36016956944242	37.0	37.0	37.0	35.0	37.0
5	36.31935295423973	37.0	37.0	37.0	35.0	37.0
6	36.35248830567763	37.0	37.0	37.0	35.0	37.0
7	36.222765984833806	37.0	37.0	37.0	35.0	37.0
8	36.27221015973871	37.0	37.0	37.0	35.0	37.0
9	38.093948939625186	39.0	39.0	39.0	37.0	39.0
10-11	38.08473889163143	39.0	39.0	39.0	36.0	39.0
12-13	38.076014863494336	39.0	39.0	39.0	35.0	39.0
14-15	39.57162243782241	41.0	40.0	41.0	37.0	41.0
16-17	39.57990514408735	41.0	39.5	41.0	37.0	41.0
18-19	39.653063014256674	41.0	40.0	41.0	37.0	41.0
20-21	39.65849650690909	41.0	40.0	41.0	37.0	41.0
22-23	39.590803955488724	41.0	40.0	41.0	37.0	41.0
24-25	39.57084842715431	41.0	40.0	41.0	37.0	41.0
26-27	39.49311127676398	41.0	40.0	41.0	36.0	41.0
28-29	39.40972676235241	41.0	39.5	41.0	36.0	41.0
30-31	39.253117613693426	41.0	39.0	41.0	35.5	41.0
32-33	39.06158533566631	41.0	39.0	41.0	35.0	41.0
34-35	38.96259698118865	41.0	39.0	41.0	35.0	41.0
36-37	38.86262201771794	41.0	39.0	41.0	35.0	41.0
38-39	38.74966356279713	41.0	39.0	41.0	35.0	41.0
40-41	38.6542818830299	41.0	38.5	41.0	35.0	41.0
42-43	38.530700159979176	41.0	38.0	41.0	35.0	41.0
44-45	38.338649470201574	41.0	38.0	41.0	35.0	41.0
46-47	38.04232234319282	41.0	38.0	41.0	34.0	41.0
48-49	37.78687675662372	40.0	38.0	41.0	33.0	41.0
50-51	37.64440744758949	40.0	38.0	41.0	33.0	41.0
52-53	37.53699239144119	40.0	38.0	41.0	33.0	41.0
54-55	37.396927720813	40.0	37.0	41.0	33.0	41.0
56-57	37.241722173736534	40.0	37.0	41.0	33.0	41.0
58-59	37.066818111736474	40.0	36.5	41.0	32.5	41.0
60-61	36.876809525762866	40.0	36.0	41.0	32.0	41.0
62-63	36.656565157921094	39.0	35.5	41.0	32.0	41.0
64-65	36.39030817562913	39.0	35.0	41.0	31.5	41.0
66-67	36.109435659655965	39.0	35.0	41.0	31.0	41.0
68-69	35.84121578517809	38.0	35.0	40.5	31.0	41.0
70-71	35.581747521454325	37.0	35.0	40.0	31.0	41.0
72-73	35.313655800058555	37.0	35.0	39.5	31.0	41.0
74-75	35.00188495361169	36.5	35.0	39.0	31.0	41.0
76-77	34.66828692145461	36.0	35.0	39.0	31.0	41.0
78-79	34.33641230778805	35.5	35.0	38.0	31.0	39.5
80-81	34.01394817579887	35.0	35.0	37.0	30.0	39.0
82-83	33.68124410333156	35.0	35.0	37.0	30.0	39.0
84-85	33.40591060120204	35.0	34.5	36.0	30.0	38.0
86-87	33.1731544753857	35.0	34.0	36.0	30.0	37.0
88-89	32.94242857163064	35.0	34.0	36.0	29.5	37.0
90-91	32.74243380525541	35.0	34.0	35.0	29.0	36.5
92-93	32.593858977858936	35.0	34.0	35.0	29.0	36.0
94-95	32.459417059070645	35.0	34.0	35.0	29.0	36.0
96-97	32.32904039368174	35.0	34.0	35.0	29.0	36.0
98-99	32.214098960771864	35.0	34.0	35.0	29.0	36.0
100	32.11310626945812	35.0	34.0	35.0	29.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2786988012243228
1101	2	0.27356109608235357
1101	3	0.2987666916226601
1101	4	0.17432568493510558
1101	5	0.21969623987860842
1101	6	0.2323718907197545
1101	7	0.25458326158829436
1101	8	0.25792379633278983
1101	9	0.3509940271799721
1101	10-11	0.36299357280103806
1101	12-13	0.32016731004956966
1101	14-15	0.4407085573661931
1101	16-17	0.4525593905858827
1101	18-19	0.39614453127374816
1101	20-21	0.4170676305316334
1101	22-23	0.4334411956533444
1101	24-25	0.40659555583106766
1101	26-27	0.41573355355073716
1101	28-29	0.4554841714928699
1101	30-31	0.43321425796058577
1101	32-33	0.4990540293382324
1101	34-35	0.4829444755341541
1101	36-37	0.4844810466042091
1101	38-39	0.4989308785776956
1101	40-41	0.4500975968822658
1101	42-43	0.42927330220945237
1101	44-45	0.4855713620084572
1101	46-47	0.4318015068269645
1101	48-49	0.41956307660066017
1101	50-51	0.4217667891661634
1101	52-53	0.35852193126277143
1101	54-55	0.40964362642282026
1101	56-57	0.4361471835826407
1101	58-59	0.4533323229460464
1101	60-61	0.4533455822504422
1101	62-63	0.4262532811978943
1101	64-65	0.45660822440500226
1101	66-67	0.43791433471822216
1101	68-69	0.40692908264214367
1101	70-71	0.37488877656665665
1101	72-73	0.4009883892767192
1101	74-75	0.37191738258532325
1101	76-77	0.376667494122664
1101	78-79	0.35479977109753236
1101	80-81	0.33633990209005304
1101	82-83	0.3371056156413488
1101	84-85	0.33262127861490143
1101	86-87	0.3680815452869055
1101	88-89	0.39242655844501684
1101	90-91	0.37377958749394935
1101	92-93	0.44073225072654765
1101	94-95	0.4370169332173717
1101	96-97	0.4519300898523255
1101	98-99	0.47971710930000455
1101	100	0.5362738387158004
1102	1	0.20808243792433956
1102	2	0.1953627233008035
1102	3	0.21622018784815822
1102	4	0.14367178151920257
1102	5	0.1427898410326236
1102	6	0.12298319243107159
1102	7	0.1786909808367838
1102	8	0.1948830126507204
1102	9	0.2131738435464925
1102	10-11	0.26982814921277054
1102	12-13	0.2852992311333651
1102	14-15	0.33705341135770794
1102	16-17	0.36695038562185545
1102	18-19	0.32692602033071694
1102	20-21	0.3320854515417224
1102	22-23	0.36952012077217944
1102	24-25	0.37319571484588465
1102	26-27	0.363290556863916
1102	28-29	0.3088086939126029
1102	30-31	0.26688131550580607
1102	32-33	0.30150958569204533
1102	34-35	0.2971427632700383
1102	36-37	0.28872140450928185
1102	38-39	0.32473978543058024
1102	40-41	0.3552928581242014
1102	42-43	0.2897788678136948
1102	44-45	0.3639246859307548
1102	46-47	0.3327963096110409
1102	48-49	0.36611320613241105
1102	50-51	0.3641932756777635
1102	52-53	0.3620277929836675
1102	54-55	0.3759693588571622
1102	56-57	0.36379801489542274
1102	58-59	0.3929899387080198
1102	60-61	0.34221893468361486
1102	62-63	0.3282027901363449
1102	64-65	0.33684236917569876
1102	66-67	0.36038436769570836
1102	68-69	0.3729667143193254
1102	70-71	0.3580745973970423
1102	72-73	0.34338534128130505
1102	74-75	0.3274850100908111
1102	76-77	0.3455785461323728
1102	78-79	0.3283153770624594
1102	80-81	0.2816251059793089
1102	82-83	0.348373480160177
1102	84-85	0.3418160693622738
1102	86-87	0.29419023810249456
1102	88-89	0.3034231855957472
1102	90-91	0.3419229566499169
1102	92-93	0.381837400258604
1102	94-95	0.37006876426250557
1102	96-97	0.37116427251635287
1102	98-99	0.39334153599192945
1102	100	0.4387342646196828
1103	1	0.16634317854904168
1103	2	0.20808297418120247
1103	3	0.21132497071604206
1103	4	0.1391044240209851
1103	5	0.15375422700792996
1103	6	0.1838271791105015
1103	7	0.13492255651445362
1103	8	0.15644059193199666
1103	9	0.2707309495316821
1103	10-11	0.26014737805477495
1103	12-13	0.24318571136689116
1103	14-15	0.3408827081180448
1103	16-17	0.3335684507378929
1103	18-19	0.3102825414906363
1103	20-21	0.32415280485591325
1103	22-23	0.340376386707014
1103	24-25	0.2926840831090729
1103	26-27	0.2655639094571285
1103	28-29	0.33734532699666886
1103	30-31	0.37461839283214715
1103	32-33	0.3145102076044495
1103	34-35	0.2535711133042895
1103	36-37	0.28531080240903606
1103	38-39	0.24899839421453862
1103	40-41	0.2532580531211863
1103	42-43	0.27863193314227885
1103	44-45	0.3313934455435401
1103	46-47	0.333255380042786
1103	48-49	0.3432810629010916
1103	50-51	0.4164814899021536
1103	52-53	0.3990084852478262
1103	54-55	0.3812772875039272
1103	56-57	0.3837642733023685
1103	58-59	0.4068535561294695
1103	60-61	0.3659613528249821
1103	62-63	0.3689805203836869
1103	64-65	0.40970490511222124
1103	66-67	0.44812313703883433
1103	68-69	0.41065073997371115
1103	70-71	0.41146864752291634
1103	72-73	0.44251543377487224
1103	74-75	0.4237683387213025
1103	76-77	0.3339963480904231
1103	78-79	0.39986567949999596
1103	80-81	0.39102453541317317
1103	82-83	0.35654043184213435
1103	84-85	0.36838161166620864
1103	86-87	0.3854862593693511
1103	88-89	0.36643164835748365
1103	90-91	0.42180451714796874
1103	92-93	0.42247948464923724
1103	94-95	0.41744552736576424
1103	96-97	0.40803843968761555
1103	98-99	0.41035521672593234
1103	100	0.4314617722605334
1104	1	0.09712067137032676
1104	2	0.1471828166450564
1104	3	0.1690544393150759
1104	4	0.0501320019003586
1104	5	0.09251121630443038
1104	6	0.14183803032985765
1104	7	0.10457588168983989
1104	8	0.11933239519193961
1104	9	0.1732131683420448
1104	10-11	0.21763727830094126
1104	12-13	0.22852605806077975
1104	14-15	0.25700709972640823
1104	16-17	0.279334988459361
1104	18-19	0.233845226676614
1104	20-21	0.2663962225039711
1104	22-23	0.2683268300097268
1104	24-25	0.2999083019327813
1104	26-27	0.2747198044825865
1104	28-29	0.30229127103655884
1104	30-31	0.3150540284042478
1104	32-33	0.26675557420239926
1104	34-35	0.2769162213447345
1104	36-37	0.27494717791497436
1104	38-39	0.23888160494033883
1104	40-41	0.2696497224801675
1104	42-43	0.2823071809067059
1104	44-45	0.2663321131861096
1104	46-47	0.34248549241475956
1104	48-49	0.33297819418025654
1104	50-51	0.3222982476310037
1104	52-53	0.32233231685474095
1104	54-55	0.25394343190919955
1104	56-57	0.2627703422084693
1104	58-59	0.19031819341035572
1104	60-61	0.21184194461956452
1104	62-63	0.2425809932780325
1104	64-65	0.2866212136772148
1104	66-67	0.3144971276591022
1104	68-69	0.26292057566151783
1104	70-71	0.30726856518097634
1104	72-73	0.2492541782318014
1104	74-75	0.22621117349514464
1104	76-77	0.251680614394175
1104	78-79	0.21174340934028635
1104	80-81	0.17303210629729193
1104	82-83	0.21308723570724908
1104	84-85	0.15928918855443186
1104	86-87	0.21141191526961478
1104	88-89	0.18370997079289708
1104	90-91	0.22112736427309443
1104	92-93	0.22561707955929222
1104	94-95	0.2515378313485783
1104	96-97	0.27659582601713595
1104	98-99	0.27935111678340263
1104	100	0.29659491555933926
1105	1	0.03702625826923622
1105	2	0.0673980044113236
1105	3	0.10677726650241226
1105	4	-0.02177677757111951
1105	5	-0.0449515323232319
1105	6	-0.09980063907099179
1105	7	-0.449732826342121
1105	8	-0.4015907480803875
1105	9	-0.37346674572432903
1105	10-11	-0.3345189255450052
1105	12-13	-0.22318558782633602
1105	14-15	-0.208537903801556
1105	16-17	-0.14083410225780568
1105	18-19	-0.17542937667661818
1105	20-21	-0.15885522153318732
1105	22-23	-0.17536240267921954
1105	24-25	-0.147036591850501
1105	26-27	-0.15922687140270142
1105	28-29	-0.18907109364332797
1105	30-31	-0.14322568365197696
1105	32-33	-0.21554246500954832
1105	34-35	-0.22522258260001138
1105	36-37	-0.1928758525593608
1105	38-39	-0.22837509602411643
1105	40-41	-0.2893800966203415
1105	42-43	-0.30031994563443476
1105	44-45	-0.2352063386525245
1105	46-47	-0.24234114738911927
1105	48-49	-0.23722321007265634
1105	50-51	-0.26627200788028205
1105	52-53	-0.26585056483061464
1105	54-55	-0.2620996893919809
1105	56-57	-0.27053375469105134
1105	58-59	-0.21462550791661528
1105	60-61	-0.22037358384850592
1105	62-63	-0.23375244466090805
1105	64-65	-0.1495725991748742
1105	66-67	-0.15686875348212936
1105	68-69	-0.1571571735050341
1105	70-71	-0.1430246992887163
1105	72-73	-0.18574188458453733
1105	74-75	-0.18043639372664444
1105	76-77	-0.17201157960884927
1105	78-79	-0.17895846492410072
1105	80-81	-0.1791394949543914
1105	82-83	-0.1894084248255936
1105	84-85	-0.14206524386420227
1105	86-87	-0.17688997809182894
1105	88-89	-0.15114117479708256
1105	90-91	-0.11540821705768423
1105	92-93	-0.12515947628650537
1105	94-95	-0.1463935075627134
1105	96-97	-0.12260672080815027
1105	98-99	-0.21933952875531304
1105	100	-0.20267633470445645
1106	1	0.12713634980801203
1106	2	0.10946647875229587
1106	3	0.08180388064490529
1106	4	0.08327414941895483
1106	5	0.10588747726370684
1106	6	0.10211733186057614
1106	7	0.14433310078214134
1106	8	0.1575331787959442
1106	9	0.16094216836571462
1106	10-11	0.16719714121792606
1106	12-13	0.16413096355042
1106	14-15	0.2459402736970162
1106	16-17	0.22667703588178512
1106	18-19	0.21551754273752977
1106	20-21	0.19246449487717143
1106	22-23	0.226905591466128
1106	24-25	0.20215481472541796
1106	26-27	0.2105969722764769
1106	28-29	0.2425613505801678
1106	30-31	0.2355522876702949
1106	32-33	0.23252690282902222
1106	34-35	0.30256904514121175
1106	36-37	0.2554315317948692
1106	38-39	0.2634819531224508
1106	40-41	0.3177161073658539
1106	42-43	0.3120014849257018
1106	44-45	0.31520195177837707
1106	46-47	0.33416802647978017
1106	48-49	0.22862692441753296
1106	50-51	0.26774339482076925
1106	52-53	0.20502641566598356
1106	54-55	0.20800306648398958
1106	56-57	0.22255624447095101
1106	58-59	0.18851361900807007
1106	60-61	0.20796164608290013
1106	62-63	0.1905560942660287
1106	64-65	0.20620144196289658
1106	66-67	0.23755550379114254
1106	68-69	0.20701262317223268
1106	70-71	0.19082100459259266
1106	72-73	0.17698007718128395
1106	74-75	0.18646375563388062
1106	76-77	0.17183883275288991
1106	78-79	0.13932126555145885
1106	80-81	0.11478829970400994
1106	82-83	0.19997518414889726
1106	84-85	0.17169288483705714
1106	86-87	0.17079520768501766
1106	88-89	0.23158423389043747
1106	90-91	0.21688284845921402
1106	92-93	0.1961867730226956
1106	94-95	0.21640188385915593
1106	96-97	0.17989204182364915
1106	98-99	0.15309077560166884
1106	100	0.1687032610161765
1107	1	-0.0386641519144959
1107	2	-0.017411962080153387
1107	3	-0.015170755779685408
1107	4	-0.05000073953672768
1107	5	-0.08342266071640836
1107	6	-0.05086073030368965
1107	7	-0.025747167111475733
1107	8	-0.04362257482824816
1107	9	-0.01525330816790671
1107	10-11	0.006970193992557938
1107	12-13	0.016907039376540922
1107	14-15	0.014176685700334701
1107	16-17	-0.010607859907402428
1107	18-19	0.009218844441541307
1107	20-21	0.03444677723931022
1107	22-23	0.04185495679872275
1107	24-25	0.047369601024513486
1107	26-27	-4.617315685209178E-4
1107	28-29	-0.012737595622745346
1107	30-31	0.03895204849791867
1107	32-33	0.015753527004704893
1107	34-35	-0.019965003525619807
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2216	46-47	-0.9318260264619695
2216	48-49	-0.8864092534680594
2216	50-51	-0.8244823121930338
2216	52-53	-0.7856176063125702
2216	54-55	-0.8898014835748498
2216	56-57	-0.8881267923843197
2216	58-59	-1.0144409135226695
2216	60-61	-0.9481395251445122
2216	62-63	-1.0096185306606245
2216	64-65	-0.9410558781303138
2216	66-67	-0.8528286429275411
2216	68-69	-0.9500377840359775
2216	70-71	-0.9299410524634268
2216	72-73	-0.9267755304066014
2216	74-75	-0.9131271614953675
2216	76-77	-0.8919701433870131
2216	78-79	-0.8414707580043199
2216	80-81	-0.8426583333061899
2216	82-83	-0.8679863127575942
2216	84-85	-0.8646645310514671
2216	86-87	-0.8200141596347166
2216	88-89	-0.8062226876180105
2216	90-91	-0.8114323693735912
2216	92-93	-0.9319313551630586
2216	94-95	-0.8899209351101902
2216	96-97	-0.9759313667274441
2216	98-99	-1.006741413874348
2216	100	-0.9588662312061551
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	52.0
3	380.0
4	165.0
5	269.0
6	324.0
7	597.0
8	1020.0
9	1377.0
10	2119.0
11	3275.0
12	5338.0
13	8330.0
14	8631.0
15	11176.0
16	14639.0
17	23569.0
18	29047.0
19	14515.0
20	8751.0
21	7815.0
22	7751.0
23	7853.0
24	8697.0
25	10423.0
26	12881.0
27	16552.0
28	20580.0
29	25969.0
30	32816.0
31	41533.0
32	52829.0
33	69608.0
34	96412.0
35	158348.0
36	276489.0
37	634382.0
38	1212428.0
39	702781.0
40	5114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.09999202150685	21.786520420698587	15.20310357725077	23.910383980543795
2	13.4672481176632	22.265141088622244	40.72224021771879	23.545370575995765
3	15.174044272139536	28.825804093505212	32.0424327242781	23.95771891007715
4	10.53067702483641	19.05811759314007	40.448124750688166	29.963080631335348
5	11.47326537165101	38.71583822158601	36.1376697922251	13.673226614537878
6	27.30008614263466	40.361120108859396	18.92767272022598	13.411121028279963
7	24.08763068148867	34.27215131682242	24.624826901397096	17.01539110029181
8	24.296296715405386	35.25276285880388	21.866055982811076	18.584884442979657
9	25.366926603363382	15.99132632782011	22.610758352228604	36.030988716587906
10-11	22.50131901489037	27.416046293532798	30.358418426885553	19.72421626469128
12-13	24.011177325108527	25.774654262504477	30.034655082910515	20.17951332947648
14-15	21.517539008185672	26.897337499487247	28.515843030862825	23.069280461464256
16-17	19.8124806391246	30.04762598537131	28.83673495368242	21.30315842182167
18-19	19.696449763567465	28.165784258671195	32.318594785895236	19.819171191866097
20-21	21.139388401438822	27.3562839566769	31.957375662513243	19.546951979371034
22-23	20.640918538442975	27.36615845783945	31.995613371268178	19.997309632449397
24-25	20.316116906845487	27.584256845057535	31.93849551244522	20.161130735651756
26-27	20.203603156315708	27.51242461598088	31.951055464673917	20.33291676302949
28-29	20.185139135130946	27.45042790244769	32.15872689862881	20.205706063792558
30-31	20.50856498563768	27.70917594485298	31.816133979881855	19.966125089627486
32-33	20.120950333750724	27.564451488312642	32.09063975914045	20.22395841879619
34-35	20.31767668001601	27.430652954911988	31.886973224790193	20.36469714028181
36-37	20.717374089840586	27.598811548305747	31.382658642939127	20.30115571891454
38-39	20.55238063714226	27.66699865746711	31.510128124119014	20.27049258127162
40-41	20.41975244026902	27.526347691213047	31.591230096450833	20.462669772067095
42-43	20.410470920992253	27.635057872852293	31.400202216917535	20.55426898923792
44-45	20.639614079249885	27.745437602321367	31.037586365808263	20.57736195262049
46-47	20.808374318526422	27.89623707620834	30.566568162635054	20.728820442630184
48-49	20.92999077305347	27.874035417725494	30.225838816857348	20.970134992363686
50-51	20.953559439605883	27.95861319373837	29.975376244979202	21.112451121676543
52-53	20.87780913080938	28.230572039511326	29.818227699203458	21.07339113047584
54-55	21.02448374589388	28.48218767915544	29.69592888113365	20.79739969381703
56-57	20.954200136131632	28.14339028871109	29.703755275383653	21.198654299773622
58-59	20.849154551446894	27.728138146038965	30.07431799728981	21.348389305224327
60-61	21.059483700114757	28.064398086977704	29.81202325263298	21.06409496027456
62-63	20.837592136549514	27.977585941069382	30.030326733779656	21.154495188601448
64-65	20.830392366263204	27.924938504908997	30.112763396311287	21.131905732516508
66-67	20.90216253704411	27.930722657661743	29.864025088784917	21.303089716509234
68-69	21.21716798517149	28.02788380258978	29.67777352798395	21.07717468425478
70-71	21.17001036846967	27.85378335980853	29.754650605204603	21.221555666517197
72-73	20.973245687489534	27.943166747387142	29.797696184712596	21.285891380410725
74-75	21.082203980572416	27.99992814238445	29.645326086464515	21.272541790578618
76-77	21.133470860218218	28.062769178889813	29.470236707683323	21.333523253208647
78-79	21.20055352581317	28.102213579884207	29.54205682961202	21.155176064690608
80-81	21.07497034135834	28.094085542778956	29.55105437938647	21.279889736476232
82-83	20.929598989247744	28.041642042405314	29.728346186042575	21.300412782304367
84-85	20.905572774029885	27.961555974218072	29.581095289226283	21.55177596252576
86-87	21.05504738987226	28.06400426385789	29.43919179071564	21.441756555554203
88-89	20.835349604862646	27.975574741706243	29.5515835694039	21.63749208402721
90-91	20.730844978239755	27.92836821276849	29.56413165603491	21.776655152956845
92-93	20.777272755563526	28.050285765370813	29.51093976945261	21.66150170961304
94-95	20.726228511316	27.796055586226814	29.641607374003005	21.836108528454183
96-97	20.707319677968965	27.86854242270145	29.58146291713493	21.84267498219465
98-99	20.534440325177673	27.855506953897237	29.562985386921177	22.047067334003913
100	20.614262903753293	27.9004093124524	29.489140262788325	21.99618752100598
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1085.0
1	769.5
2	1902.5
3	2875.0
4	1496.0
5	780.0
6	1755.0
7	4659.0
8	7970.0
9	8469.0
10	5607.0
11	2956.0
12	2526.5
13	2774.0
14	3143.0
15	3495.5
16	3873.5
17	4371.0
18	5036.0
19	5797.5
20	6829.5
21	8364.5
22	10456.5
23	13802.0
24	18187.0
25	23528.5
26	30614.5
27	39820.5
28	51177.5
29	63073.0
30	73490.0
31	82235.0
32	90526.5
33	98410.5
34	106098.0
35	113367.5
36	119134.5
37	123667.5
38	127935.0
39	130440.5
40	130854.0
41	130289.5
42	128569.0
43	128335.5
44	126380.5
45	120520.0
46	116240.5
47	113340.5
48	108899.5
49	105138.5
50	103679.0
51	98329.5
52	89034.5
53	81400.5
54	75211.5
55	70428.5
56	63689.5
57	55586.0
58	50401.0
59	46325.5
60	40418.0
61	33594.0
62	27900.0
63	22426.5
64	17358.0
65	13252.5
66	9994.5
67	7432.0
68	5367.5
69	3794.0
70	2608.5
71	1861.0
72	1319.0
73	875.0
74	639.5
75	477.0
76	308.5
77	218.0
78	149.0
79	72.5
80	43.0
81	24.0
82	14.5
83	6.5
84	7.0
85	6.5
86	3.5
87	3.5
88	4.5
89	3.0
90	1.5
91	0.5
92	0.0
93	3.5
94	5.0
95	6.0
96	8.5
97	10.5
98	7.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009420524578940744
2	0.0
3	4.526378176067624E-4
4	0.0029704356780443785
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.65797272008453E-5
24-25	9.901452260147927E-5
26-27	4.950726130073964E-4
28-29	9.618553624143701E-4
30-31	0.0018529860658276836
32-33	0.004554668039668046
34-35	0.005756987242686009
36-37	0.004300059267264243
38-39	0.002786551564641631
40-41	0.0037484069270560015
42-43	4.8092768120718503E-4
44-45	4.526378176067624E-4
46-47	0.003493798154652197
48-49	0.0022490441562336007
50-51	0.0010467249532156381
52-53	1.4144931800211325E-5
54-55	5.799422038086643E-4
56-57	5.940871356088757E-4
58-59	7.072465900105663E-4
60-61	2.687537042040152E-4
62-63	0.0
64-65	0.0
66-67	8.062611126120456E-4
68-69	6.50666862809721E-4
70-71	0.002263189088033812
72-73	8.486959080126795E-5
74-75	0.0018954208612283177
76-77	3.1118849960464916E-4
78-79	0.0011457394758171173
80-81	8.628408398128908E-4
82-83	0.0011881742712177513
84-85	9.477104306141589E-4
86-87	2.263189088033812E-4
88-89	0.0014569279754217664
90-91	0.0017964063386268385
92-93	0.00330991404124945
94-95	0.001089159748616272
96-97	0.002333913747034869
98-99	0.00256023265583825
100	0.003592812677253677
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3534835.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.79353104331028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.41750388027027	47.22107398277765
2	15.05567256887188	18.60686340524999
3	4.300518758404039	7.972327181993346
4	1.6588101421192971	4.100149440480268
5	0.783241030357159	2.4199614461884713
6	0.43266934519051464	1.6041699968111278
7	0.27343270141755777	1.1827460486311432
8	0.1806722093250023	0.8931499020470387
9	0.14536925477394955	0.8084591601855238
>10	0.6666532856762893	7.401865257138658
>50	0.05405587392839042	2.3082018214094386
>100	0.029978335348490353	3.412435183693068
>500	8.260341247698912E-4	0.359006408158037
>1k	4.589078402742593E-4	0.5465347888997157
>5k	4.5890784027425884E-5	0.27376365132765285
>10k+	9.178156805485177E-5	0.8892923250088247
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17710	0.5010134843634851	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13650	0.38615663814576917	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9654	0.27311034319848027	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4284	0.12119377566421063	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.828986360042265E-5	0.0
4	0.0	0.0	0.0	2.828986360042265E-5	0.0
5	0.0	0.0	0.0	2.828986360042265E-5	0.0
6	0.0	0.0	0.0	2.828986360042265E-5	0.0
7	0.0	0.0	0.0	2.828986360042265E-5	0.0
8	0.0	0.0	0.0	2.828986360042265E-5	0.0
9	0.0	0.0	0.0	2.828986360042265E-5	0.0
10-11	0.0	0.0	0.0	4.2434795400633975E-5	0.0
12-13	0.0	0.0	0.0	5.65797272008453E-5	0.0
14-15	0.0	0.0	0.0	5.65797272008453E-5	0.0
16-17	0.0	0.0	0.0	8.486959080126795E-5	0.0
18-19	0.0	0.0	0.0	8.486959080126795E-5	0.0
20-21	0.0	0.0	0.0	1.4144931800211326E-4	0.0
22-23	0.0	0.0	0.0	2.1217397700316987E-4	0.0
24-25	0.0	0.0	0.0	4.102030222061284E-4	0.0
26-27	0.0	0.0	0.0	8.769857716131022E-4	0.0
28-29	0.0	0.0	0.0	0.0021075948382314877	0.0
30-31	0.0	0.0	0.0	0.005601392992883685	0.0
32-33	0.0	0.0	0.0	0.012489974779586601	0.0
34-35	0.0	0.0	0.0	0.02421612324196179	0.0
36-37	0.0	0.0	0.0	0.041487084970019815	0.0
38-39	0.0	0.0	0.0	0.06366633803275118	0.0
40-41	0.0	0.0	0.0	0.08782588154751211	0.0
42-43	0.0	0.0	0.0	0.11226832369827729	0.0
44-45	0.0	0.0	0.0	0.14241117336452763	0.0
46-47	0.0	0.0	0.0	0.1803478804526944	0.0
48-49	0.0	0.0	0.0	0.22054777662889496	0.0
50-51	0.0	0.0	0.0	0.26090326705489786	0.0
52-53	0.0	0.0	0.0	0.30159823584410583	0.0
54-55	0.0	0.0	0.0	0.3474843946039914	0.0
56-57	0.0	0.0	0.0	0.40318713603322365	0.0
58-59	0.0	0.0	0.0	0.46781532942838916	0.0
60-61	0.0	0.0	0.0	0.537083060454024	0.0
62-63	0.0	0.0	0.0	0.6091090531807001	0.0
64-65	0.0	0.0	0.0	0.6873163811040686	0.0
66-67	0.0	0.0	0.0	0.7771932777626113	0.0
68-69	0.0	0.0	0.0	0.883930933127006	0.0
70-71	0.0	0.0	0.0	1.003483896702392	0.0
72-73	0.0	0.0	0.0	1.1283694995664577	0.0
74-75	0.0	0.0	0.0	1.260907510534438	0.0
76-77	0.0	0.0	0.0	1.4036581622621707	0.0
78-79	0.0	0.0	0.0	1.5633402973547563	0.0
80-81	0.0	0.0	0.0	1.753617919931199	0.0
82-83	0.0	0.0	0.0	1.9558480098788205	0.0
84-85	0.0	0.0	0.0	2.1626044780025095	0.0
86-87	0.0	0.0	0.0	2.382940080654401	0.0
88	0.0	0.0	0.0	2.56091161256466	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7710	0.0	57.184776	1
GTATCAA	15340	0.0	44.889446	1
ATCAACG	20155	0.0	33.836056	3
TCAACGC	20330	0.0	33.567913	4
TATCAAC	20830	0.0	32.829845	2
CAACGCA	20935	0.0	32.61936	5
AACGCAG	21695	0.0	31.455006	6
ACGCAGA	24230	0.0	28.164108	7
CGCAGAG	24305	0.0	28.038528	8
GCAGAGT	26095	0.0	25.845053	9
TAGACCG	110	1.019162E-4	25.635477	5
GTGGTAT	3115	0.0	24.143148	1
GTACATG	14835	0.0	22.685976	1
TGGTATC	3285	0.0	22.319492	2
AGAGTAC	25115	0.0	22.231363	10-11
TACATGG	14620	0.0	22.149654	2
GAGTACT	17275	0.0	21.764803	12-13
AGTACTT	18005	0.0	21.600197	12-13
ACATGGG	14965	0.0	21.010893	3
CAGAGTA	25995	0.0	20.818861	10-11
>>END_MODULE