FastQCFastQC Report
Wed 25 May 2016
SRR2431354_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431354_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3523905
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA277670.7879610829463337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56600.046.9164731
GTATCAA100550.035.8514441
TCAACGC116250.029.2306634
ATCAACG116850.029.2012393
TATCAAC132850.026.7456742
CAACGCA131700.025.8015545
AACGCAG135600.025.0594716
GTACATG206450.024.6778721
TACATGG200850.023.985312
ACGCAGA142900.023.3846427
CGCAGAG142950.023.2120728
ACATGGG213600.021.6074523
GCAGAGT152700.020.621929
CATGGGG111750.019.5989024
TCTGTCG7700.018.921888
CATGGGT30650.018.247774
CAGAGTA151400.017.71018610-11
GTGGTAT29800.017.3488141
TATACTG13650.017.2159375
CATGGGA69500.017.1091754
Produced by FastQC (version 0.11.5)