##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431354_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3523905
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.916220499701325	33.0	31.0	34.0	26.0	34.0
2	31.008017526011628	34.0	31.0	34.0	27.0	34.0
3	31.03482358349615	34.0	31.0	34.0	27.0	34.0
4	34.171557405775694	37.0	35.0	37.0	30.0	37.0
5	34.37514149785536	37.0	35.0	37.0	31.0	37.0
6	34.33173539014247	37.0	35.0	37.0	31.0	37.0
7	34.30660304406617	37.0	35.0	37.0	31.0	37.0
8	34.248573102850386	37.0	35.0	37.0	30.0	37.0
9	35.672962523110016	39.0	37.0	39.0	30.0	39.0
10-11	35.737245470578806	39.0	37.0	39.0	30.0	39.0
12-13	35.68262609236061	39.0	37.0	39.0	30.0	39.0
14-15	37.02947312711325	41.0	38.0	41.0	30.0	41.0
16-17	37.04080884700354	41.0	38.0	41.0	30.0	41.0
18-19	37.062345466180275	41.0	38.0	41.0	30.0	41.0
20-21	37.125563827628724	41.0	38.0	41.0	30.0	41.0
22-23	37.15203928596259	40.5	38.0	41.0	30.0	41.0
24-25	37.184495325498276	40.5	38.0	41.0	30.0	41.0
26-27	37.20313686095397	40.0	38.0	41.0	30.0	41.0
28-29	37.18842221910069	40.0	38.0	41.0	30.0	41.0
30-31	37.1177981529014	40.0	38.0	41.0	30.0	41.0
32-33	37.034433391365546	40.0	38.0	41.0	30.0	41.0
34-35	36.96090884969941	40.0	38.0	41.0	30.0	41.0
36-37	36.85174259805528	40.0	37.5	41.0	30.0	41.0
38-39	36.7359664917187	40.0	37.0	41.0	29.0	41.0
40-41	36.6287401334599	40.0	37.0	41.0	29.0	41.0
42-43	36.476639126196645	40.0	37.0	41.0	28.0	41.0
44-45	36.322884129963775	40.0	36.0	41.0	28.0	41.0
46-47	36.169683632220504	40.0	36.0	41.0	28.0	41.0
48-49	36.01616119049747	40.0	35.5	41.0	28.0	41.0
50-51	35.85198352395993	40.0	35.0	41.0	27.5	41.0
52-53	35.643908533289064	39.0	35.0	41.0	27.0	41.0
54-55	35.43766843884838	39.0	35.0	41.0	27.0	41.0
56-57	35.20069624464905	39.0	35.0	41.0	27.0	41.0
58-59	34.93559942733984	38.5	34.0	40.5	26.5	41.0
60-61	34.716741796387815	38.0	34.0	40.0	26.0	41.0
62-63	34.50567992042919	38.0	34.0	40.0	26.0	41.0
64-65	34.20467237340394	37.0	34.0	40.0	26.0	41.0
66-67	33.86463028940905	36.5	34.0	40.0	25.5	41.0
68-69	33.52528274740664	36.0	33.5	39.0	25.0	41.0
70-71	33.25974536770997	36.0	33.0	39.0	24.5	41.0
72-73	33.005993067350005	35.0	33.0	39.0	24.0	41.0
74-75	32.66482964211578	35.0	33.0	37.5	23.5	40.0
76-77	32.33952731415859	35.0	33.0	37.0	23.0	39.5
78-79	32.024066483063535	35.0	33.0	37.0	22.5	39.0
80-81	31.71961971165511	35.0	33.0	36.0	21.5	39.0
82-83	31.434912831078023	35.0	32.0	36.0	20.0	37.5
84-85	31.179887085491806	35.0	32.0	35.5	20.0	37.0
86-87	30.952758942139475	35.0	32.0	35.0	19.0	37.0
88-89	30.736130514301607	35.0	32.0	35.0	18.0	36.0
90-91	30.547851602128887	35.0	32.0	35.0	16.0	36.0
92-93	30.370151720889183	35.0	31.5	35.0	8.5	36.0
94-95	30.163673963968947	35.0	31.0	35.0	3.5	35.5
96-97	29.991196272317218	35.0	31.0	35.0	2.0	35.0
98-99	29.88027443986146	35.0	31.0	35.0	2.0	35.0
100	29.84041312123908	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6021912608403603
1101	2	0.6471604764896632
1101	3	0.6325187593414157
1101	4	0.6687861432695996
1101	5	0.5164920224409997
1101	6	0.537480847139534
1101	7	0.6438689466690519
1101	8	0.596462005929375
1101	9	0.6564022895765547
1101	10-11	0.6221184317860065
1101	12-13	0.6449271063868167
1101	14-15	0.7412586498110869
1101	16-17	0.7125061488002018
1101	18-19	0.7752684927952416
1101	20-21	0.6816071970092494
1101	22-23	0.6507360251342078
1101	24-25	0.7377178979271903
1101	26-27	0.7440188265653589
1101	28-29	0.7675496658374996
1101	30-31	0.7970682925348953
1101	32-33	0.813212783294766
1101	34-35	0.8149958477561725
1101	36-37	0.8761757790665214
1101	38-39	0.8052650256783735
1101	40-41	0.8456744176496684
1101	42-43	0.8058397232076899
1101	44-45	0.8308272574858293
1101	46-47	0.8404341261265529
1101	48-49	0.8102034066487604
1101	50-51	0.8081742514581052
1101	52-53	0.8664254242660476
1101	54-55	0.848565548016694
1101	56-57	0.8258661053682914
1101	58-59	0.8374162343527303
1101	60-61	0.9043165059057969
1101	62-63	0.85972497846317
1101	64-65	0.8819882433391513
1101	66-67	0.7984506584159092
1101	68-69	0.810434446260679
1101	70-71	0.8115990241785553
1101	72-73	0.8583629377077528
1101	74-75	0.8528942914880631
1101	76-77	0.7850659649567007
1101	78-79	0.7875757496432598
1101	80-81	0.7814030912973706
1101	82-83	0.8208253978663471
1101	84-85	0.8145323684716743
1101	86-87	0.763675223526139
1101	88-89	0.8081817182747137
1101	90-91	0.7478836847842558
1101	92-93	0.7842435562399466
1101	94-95	0.8038902971021145
1101	96-97	0.7650429027656998
1101	98-99	0.7697460259848192
1101	100	0.7958788991585379
1102	1	0.1916875007011214
1102	2	0.23409920467991086
1102	3	0.26935906811368326
1102	4	0.19510463009841317
1102	5	0.2532248062576201
1102	6	0.21230692141882912
1102	7	0.3106801575264342
1102	8	0.26133045330085736
1102	9	0.367239770158875
1102	10-11	0.30948900129509127
1102	12-13	0.27038934925488434
1102	14-15	0.30839805808051324
1102	16-17	0.3435715315362984
1102	18-19	0.2324003180923242
1102	20-21	0.24479626976697233
1102	22-23	0.13070595695711518
1102	24-25	0.19255492870589563
1102	26-27	0.25056598180631795
1102	28-29	0.3006872301111443
1102	30-31	0.2654820282633352
1102	32-33	0.23590477828323486
1102	34-35	0.3099400352884558
1102	36-37	0.3540153633763339
1102	38-39	0.32640355623367157
1102	40-41	0.36820689157947584
1102	42-43	0.33244269589206965
1102	44-45	0.32927421598746776
1102	46-47	0.3164917865706087
1102	48-49	0.32384268107174563
1102	50-51	0.26821462431743015
1102	52-53	0.34069008990373817
1102	54-55	0.31973756505747986
1102	56-57	0.26663470728512806
1102	58-59	0.2330651556699408
1102	60-61	0.23576541587839728
1102	62-63	0.28346473224545576
1102	64-65	0.29998678147742197
1102	66-67	0.3571318680691675
1102	68-69	0.33184676490872533
1102	70-71	0.3203399671540055
1102	72-73	0.31341180906889576
1102	74-75	0.2946183528962152
1102	76-77	0.23798448880474865
1102	78-79	0.18357396535593296
1102	80-81	0.2251104681760694
1102	82-83	0.16524298180397423
1102	84-85	0.20768249142131268
1102	86-87	0.23052783223780082
1102	88-89	0.17989053910260822
1102	90-91	0.22917951723439955
1102	92-93	0.2351821232173954
1102	94-95	0.1899068781369202
1102	96-97	0.19367551472151945
1102	98-99	0.1963397189819176
1102	100	0.15420295280458518
1103	1	0.06390297255207145
1103	2	0.034652893763279735
1103	3	0.09850301381837667
1103	4	0.09375896491989266
1103	5	0.03448266300052438
1103	6	0.10333541542522084
1103	7	0.0773475298639994
1103	8	0.09521741007375795
1103	9	0.13468731911766696
1103	10-11	0.1475448730349811
1103	12-13	0.23360924845226094
1103	14-15	0.2887922228714075
1103	16-17	0.1950322785102827
1103	18-19	0.17650986972544302
1103	20-21	0.19613176836121227
1103	22-23	0.11472230587494181
1103	24-25	0.10742508854200139
1103	26-27	0.14632070668329078
1103	28-29	0.1221062538827411
1103	30-31	0.09141521238829853
1103	32-33	0.0517631153418634
1103	34-35	0.0948025164404811
1103	36-37	0.08688672022721278
1103	38-39	0.0418933771770611
1103	40-41	0.07263056194778983
1103	42-43	0.08926386674123421
1103	44-45	0.03173673665705934
1103	46-47	-0.013634313537508547
1103	48-49	0.009053271321143086
1103	50-51	-0.08981509821276745
1103	52-53	-0.07462451951890614
1103	54-55	-0.019596309509026355
1103	56-57	-0.12387278004725744
1103	58-59	-0.08503504040810128
1103	60-61	-0.14836850929947332
1103	62-63	-0.13213678163197073
1103	64-65	0.01788322739054138
1103	66-67	0.03044209806537168
1103	68-69	0.03509967801667102
1103	70-71	-0.03588032492153559
1103	72-73	-0.13093853224606988
1103	74-75	-0.07512082949707377
1103	76-77	-0.04489604176787054
1103	78-79	-0.06501580057795309
1103	80-81	-0.066639237903793
1103	82-83	-0.032136328778527457
1103	84-85	-0.09472645569800875
1103	86-87	-0.06456872323482443
1103	88-89	-0.13081557981902137
1103	90-91	-0.10214400792982303
1103	92-93	-0.13926175067297564
1103	94-95	-0.20631892309495115
1103	96-97	-0.2735264607631436
1103	98-99	-0.27293352368419477
1103	100	-0.24693197934926303
1104	1	0.1265013977489211
1104	2	0.1957441532229467
1104	3	0.15883335238829233
1104	4	0.1301826686425045
1104	5	0.1210390891927915
1104	6	0.18292072151641747
1104	7	0.14706192131871632
1104	8	0.12891168896960892
1104	9	0.22125586899706207
1104	10-11	0.20393612994229215
1104	12-13	0.2355088387992481
1104	14-15	0.18811903919116446
1104	16-17	0.22929445643497104
1104	18-19	0.17868830487647358
1104	20-21	0.18549833761298373
1104	22-23	0.12668359940855112
1104	24-25	0.21544139809163454
1104	26-27	0.17475537915230888
1104	28-29	0.18046569568266335
1104	30-31	0.1664190409210775
1104	32-33	0.13119033493374133
1104	34-35	0.12169875682128861
1104	36-37	0.1367586239013292
1104	38-39	0.1011672002222781
1104	40-41	0.09281283201249835
1104	42-43	0.08873792205077535
1104	44-45	0.14862359713255557
1104	46-47	0.05412417623393395
1104	48-49	0.07469649673239331
1104	50-51	0.05318793908990216
1104	52-53	0.037736696167876005
1104	54-55	0.02073999519114267
1104	56-57	-0.037559784730831325
1104	58-59	-0.018879270171119344
1104	60-61	0.025733699763932805
1104	62-63	0.01821751717224629
1104	64-65	0.07073149625563246
1104	66-67	0.06193305634712232
1104	68-69	0.0048383168456567205
1104	70-71	-0.06252643449413853
1104	72-73	-0.0740403137899861
1104	74-75	-0.016343280013281003
1104	76-77	-0.06493298710918793
1104	78-79	-0.021490009028795498
1104	80-81	0.027361451232110312
1104	82-83	-0.02200826435707981
1104	84-85	-0.035028144080961
1104	86-87	-0.021665387306594397
1104	88-89	0.012237706976367946
1104	90-91	0.03819867765830409
1104	92-93	-0.0216875090415094
1104	94-95	0.021050460128417825
1104	96-97	-0.01956552455926186
1104	98-99	-0.012789507042839432
1104	100	0.0026564533770354615
1105	1	0.05453051941433884
1105	2	0.08393521522039293
1105	3	0.09817278856512957
1105	4	0.012620308619659681
1105	5	0.0468946744817913
1105	6	0.08593155390320106
1105	7	0.05437458455855193
1105	8	0.0255909014396849
1105	9	0.08419505233264601
1105	10-11	0.04780537122600492
1105	12-13	0.10042222141737511
1105	14-15	0.08251866955521336
1105	16-17	0.08901849688956531
1105	18-19	0.024753863918761
1105	20-21	0.0567115745586122
1105	22-23	0.13245218227823585
1105	24-25	0.26630933666532997
1105	26-27	0.26282756083864456
1105	28-29	0.24473053085454666
1105	30-31	0.23524424354703655
1105	32-33	0.3103039787355968
1105	34-35	0.3089657289510015
1105	36-37	0.28551399036072667
1105	38-39	0.3018245714432979
1105	40-41	0.24056397268591923
1105	42-43	0.2659266994927094
1105	44-45	0.2897112637414736
1105	46-47	0.3184199748336809
1105	48-49	0.30866177277215456
1105	50-51	0.2795184841104046
1105	52-53	0.23455853292482942
1105	54-55	0.2065998158920337
1105	56-57	0.1613628323686811
1105	58-59	0.19493587335159646
1105	60-61	0.19526504502059083
1105	62-63	0.25328215533330933
1105	64-65	0.21941482862636974
1105	66-67	0.22772873622699308
1105	68-69	0.1652505315168682
1105	70-71	0.25136066290107806
1105	72-73	0.21159859167690342
1105	74-75	0.17664992086717035
1105	76-77	0.15903695750959912
1105	78-79	0.14433470909170865
1105	80-81	0.15295312543909034
1105	82-83	0.2141500405797423
1105	84-85	0.18631769013475008
1105	86-87	0.16808916736551183
1105	88-89	0.20369228218380542
1105	90-91	0.2270006657349768
1105	92-93	0.19823745678641558
1105	94-95	0.16992759467975205
1105	96-97	0.17745011437829206
1105	98-99	0.13796749261704377
1105	100	0.1442130635073724
1106	1	0.19781827715831213
1106	2	0.1964206984839123
1106	3	0.19321548199950556
1106	4	0.20272189247280892
1106	5	0.14263822808200644
1106	6	0.20060332731253538
1106	7	0.15517432201603043
1106	8	0.14325427300396143
1106	9	0.15234272886766576
1106	10-11	0.12615623968711986
1106	12-13	0.1513118597069294
1106	14-15	0.1721038118040994
1106	16-17	0.18083284372421815
1106	18-19	0.1915722070187229
1106	20-21	0.24495958762761205
1106	22-23	0.21171492582336526
1106	24-25	0.1597352228977087
1106	26-27	0.23462861100872345
1106	28-29	0.2648552839640672
1106	30-31	0.23181154247131275
1106	32-33	0.1887064807609775
1106	34-35	0.2387830602692418
1106	36-37	0.2827815439560908
1106	38-39	0.25235459126719206
1106	40-41	0.24063299908670643
1106	42-43	0.14879601192920688
1106	44-45	0.17480440699789312
1106	46-47	0.23022542695054682
1106	48-49	0.20274636159884096
1106	50-51	0.11235288977493241
1106	52-53	0.048977497141301285
1106	54-55	0.09607431911388176
1106	56-57	0.164763517909833
1106	58-59	0.19170048787547245
1106	60-61	0.13861964938427462
1106	62-63	0.16287096688033387
1106	64-65	0.1868331564678698
1106	66-67	0.22202361337816257
1106	68-69	0.20560743736606213
1106	70-71	0.15757058671773194
1106	72-73	0.10044050426525786
1106	74-75	0.13215006860788492
1106	76-77	0.11856039076167235
1106	78-79	0.12259994545868835
1106	80-81	0.10365253241754147
1106	82-83	0.11489635826345435
1106	84-85	0.09038968769823441
1106	86-87	0.14138245933144233
1106	88-89	0.1252703437771565
1106	90-91	0.1403201574584685
1106	92-93	0.1823137018955343
1106	94-95	0.22377272850014762
1106	96-97	0.2182475933403758
1106	98-99	0.2272085624778164
1106	100	0.2419539928933645
1107	1	0.08471348733849027
1107	2	0.1725963724074866
1107	3	0.17886631171306533
1107	4	0.182580879955907
1107	5	0.16633710300897064
1107	6	0.14357845624919463
1107	7	0.16812074501723373
1107	8	0.19674026885823537
1107	9	0.13491091868759497
1107	10-11	0.13162821539218328
1107	12-13	0.2053247164832186
1107	14-15	0.14676766375383465
1107	16-17	0.18680506436417943
1107	18-19	0.22050151332947365
1107	20-21	0.19790950698624954
1107	22-23	0.2672031220229911
1107	24-25	0.26068939077819664
1107	26-27	0.254630067598292
1107	28-29	0.2704642726677733
1107	30-31	0.2481083249002296
1107	32-33	0.22555009322244501
1107	34-35	0.30319927571864014
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2216	50-51	-0.6836137812658265
2216	52-53	-0.6770553139609277
2216	54-55	-0.6992545725567965
2216	56-57	-0.5922306507503521
2216	58-59	-0.5913128824293068
2216	60-61	-0.4808256682127876
2216	62-63	-0.6243690322294668
2216	64-65	-0.6337762036102959
2216	66-67	-0.6647709231899697
2216	68-69	-0.6977474106911572
2216	70-71	-0.7178363997741641
2216	72-73	-0.7023034229935305
2216	74-75	-0.6866737888607091
2216	76-77	-0.628115165533913
2216	78-79	-0.6250305358289268
2216	80-81	-0.5915737968122201
2216	82-83	-0.6410400433371386
2216	84-85	-0.6387557138905962
2216	86-87	-0.6190081208598279
2216	88-89	-0.7301799629357966
2216	90-91	-0.7319841552030937
2216	92-93	-0.7665083088841023
2216	94-95	-0.7883497495846186
2216	96-97	-0.8668963273226815
2216	98-99	-0.9330835055347606
2216	100	-0.857356274835599
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74153.0
3	15015.0
4	10762.0
5	9765.0
6	6580.0
7	6948.0
8	8192.0
9	8383.0
10	8234.0
11	8999.0
12	10696.0
13	11976.0
14	12672.0
15	12976.0
16	12056.0
17	10992.0
18	10192.0
19	9393.0
20	8924.0
21	9739.0
22	12181.0
23	22120.0
24	37572.0
25	42510.0
26	39813.0
27	28888.0
28	31118.0
29	35542.0
30	41651.0
31	50460.0
32	66392.0
33	89093.0
34	129108.0
35	214646.0
36	395323.0
37	735554.0
38	924379.0
39	359338.0
40	1570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57580611886122	21.232665823091576	13.599909872354168	22.59161818569303
2	16.239327881422355	22.246560772688913	38.484895436933265	23.029215908955468
3	17.996342126135637	29.16227877879795	28.861220719627802	23.980158375438613
4	12.574470005125002	19.52367006800984	38.25898109538801	29.642878831477155
5	12.77642274692422	40.488520547517595	33.621110671258165	13.113946034300017
6	27.92291506155813	40.34595143739686	18.218567186118808	13.512566314926197
7	25.54504165123634	35.120668689990225	22.39765260414228	16.936637054631156
8	24.52793137158919	36.77329553435748	20.193138010247154	18.505635083806173
9	25.824107063045115	18.920714377941515	20.472458820541416	34.782719738471954
10-11	23.409172494718216	29.20104259337298	27.53661066345432	19.853174248454483
12-13	24.139030422216262	27.666949023881177	26.861322879021994	21.332697674880567
14-15	22.3070428970134	28.621444108169776	24.89161881492265	24.17989417989418
16-17	21.531454451808436	30.981595701359716	25.5752638053523	21.911686041479552
18-19	21.54274874038886	30.00899570221104	27.369310466655598	21.0789450907445
20-21	23.84011487256325	28.68950780455205	26.43455200977325	21.03582531311145
22-23	23.24980383977434	28.82313796768074	26.4483009615753	21.47875723096962
24-25	22.338343400290302	29.137292293634477	26.68441686140801	21.83994744466721
26-27	22.191177117430804	29.17239539658419	26.69691719839212	21.939510287592885
28-29	22.063790028391796	28.93243433066442	27.287909293808998	21.71586634713478
30-31	22.58224895393037	29.354210740641417	26.54591993825032	21.51762036717789
32-33	21.97071146923654	29.26300510371307	26.851958267887472	21.914325159162917
34-35	22.475449820582565	28.57321636082698	27.08089463251705	21.870439186073405
36-37	22.639784557188687	29.017879312864565	26.478466360472257	21.86386976947449
38-39	22.35942796414773	29.11822254005145	26.704763607418474	21.81758588838235
40-41	22.230026064834323	28.63919430291112	26.964276846282747	22.16650278597181
42-43	21.940957545677307	28.96244365270914	27.14000519310254	21.956593608511014
44-45	22.181500352591797	28.890917887968037	26.874404957000824	22.05317680243934
46-47	22.274295135652068	28.7553154809792	26.90862835405608	22.061761029312652
48-49	21.8960357898411	28.706293160570446	27.03150340318482	22.366167646403635
50-51	22.11655819325436	28.723688635193064	26.787910003249237	22.371843168303347
52-53	22.20833138237268	28.84530088069911	26.639055252624573	22.307312484303633
54-55	22.188381922895196	29.183363342655376	26.629648075075803	21.99860665937362
56-57	22.219838503024345	28.84335701444846	26.5826263761367	22.354178106390496
58-59	22.30173628403717	28.214040957403792	26.965043041739207	22.519179716819835
60-61	22.4913554707065	28.826472336796822	26.63990658090953	22.042265611587144
62-63	22.065350796914217	28.900211555078815	26.769223347394437	22.26521430061253
64-65	22.35666431936507	28.744209725320307	26.705959256273832	22.193166699040795
66-67	22.070600654671452	28.696133976369964	26.691653151830142	22.54161221712844
68-69	22.45941647121588	28.87983643145885	26.75482171057392	21.905925386751345
70-71	22.49121358265901	28.614974013204105	26.74213691912807	22.151675485008816
72-73	22.249620804193075	28.687138274158926	26.684402672603262	22.378838249044737
74-75	22.26342651121412	28.837014618725533	26.624979958313293	22.274578911747053
76-77	22.377674767055296	28.687422050253907	26.391701819430434	22.54320136326036
78-79	22.495299958426802	28.624906176528597	26.679578478988507	22.200215386056094
80-81	22.18553312100257	28.95373583478213	26.728491081412674	22.132239962802625
82-83	22.194897698572603	28.981043729958284	26.807003603961522	22.01705496750759
84-85	22.107471700554772	28.543537209661007	26.748227413758684	22.600763676025537
86-87	22.52054398004451	28.849459077697144	26.52831390733269	22.101683034925653
88-89	22.142555518537208	28.85336420171759	26.63216865534662	22.371911624398585
90-91	22.045434132341903	28.69618618172489	26.615920378102704	22.6424593078305
92-93	22.16754052694967	28.953456298094004	26.547507426582683	22.331495748373644
94-95	22.217221343284642	28.475548587117693	26.857221910849717	22.450008158747952
96-97	22.270269595330316	28.87173839198578	26.562082752408813	22.295909260275092
98-99	21.9446630759545	28.895272124929427	26.586707837971563	22.573356961144512
100	21.976322071588868	28.712028078874212	26.734752487947627	22.57689736158929
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25079.0
1	17085.5
2	7179.0
3	4032.5
4	2222.5
5	1479.0
6	1275.5
7	1486.5
8	1936.0
9	2066.0
10	1544.0
11	987.0
12	879.0
13	908.0
14	985.5
15	1101.0
16	1280.5
17	1588.5
18	1995.5
19	2565.0
20	3468.0
21	4652.5
22	6079.0
23	8189.0
24	11432.5
25	15234.5
26	19795.5
27	25662.0
28	32367.5
29	39942.0
30	46950.0
31	52935.5
32	59391.5
33	65565.0
34	71827.0
35	80058.0
36	86749.0
37	93640.5
38	102114.5
39	105865.5
40	111121.0
41	117766.0
42	121739.0
43	131649.0
44	136454.5
45	133366.0
46	133862.5
47	134691.0
48	134530.0
49	134431.5
50	134711.5
51	127841.5
52	116539.0
53	107499.5
54	101024.5
55	97018.0
56	86901.5
57	74730.0
58	67482.0
59	62099.0
60	54417.5
61	45059.5
62	37067.0
63	29169.5
64	22265.5
65	17548.0
66	13774.5
67	10418.0
68	7694.0
69	5707.0
70	4194.5
71	3196.5
72	2516.0
73	1863.0
74	1466.0
75	1147.0
76	815.5
77	626.0
78	471.0
79	321.0
80	239.5
81	196.5
82	164.0
83	138.5
84	121.5
85	109.5
86	94.0
87	69.5
88	58.0
89	62.0
90	59.0
91	47.5
92	36.0
93	33.5
94	25.0
95	19.0
96	20.5
97	20.5
98	21.5
99	21.0
100	55.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.972865329797483E-4
2	8.513282849566035E-5
3	0.0
4	8.513282849566035E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	1.4188804749276725E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.4188804749276725E-5
82-83	1.4188804749276727E-4
84-85	8.087618707087734E-4
86-87	0.0017736005936595908
88-89	0.003603956406316288
90-91	0.0028519497546046217
92-93	0.002185075931388616
94-95	0.0022702087598842763
96-97	0.0019296774459016347
98-99	0.002596551269117641
100	0.0033201803113307535
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3523905.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.393791024714076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.48382319379755	37.53522220749986
2	14.138210184394062	14.249560189916771
3	4.915505764181735	7.431329107828555
4	2.2015864954628173	4.437851591007433
5	1.196057992387378	3.0136948260904295
6	0.7176667805166519	2.16995698576413
7	0.4689273412959469	1.6541718510129904
8	0.31579517195332657	1.273129272162367
9	0.23402878838940333	1.0614238070276363
>10	1.1269110033598004	10.254366802870743
>50	0.10452117792365076	3.679739784583106
>100	0.08873667280265064	8.916376122592863
>500	0.006143892161824221	2.125201978690367
>1k	0.0020291753901011344	1.409255067712572
>5k	0.0	0.0
>10k+	5.636598305836485E-5	0.7887204052400577
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27767	0.7879610829463337	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.837760949855345E-5	0.0
4	0.0	0.0	0.0	2.837760949855345E-5	0.0
5	0.0	0.0	0.0	2.837760949855345E-5	0.0
6	0.0	0.0	0.0	2.837760949855345E-5	0.0
7	0.0	0.0	0.0	2.837760949855345E-5	0.0
8	0.0	0.0	0.0	8.513282849566035E-5	0.0
9	2.837760949855345E-5	0.0	0.0	8.513282849566035E-5	0.0
10-11	2.837760949855345E-5	0.0	0.0	8.513282849566035E-5	0.0
12-13	2.837760949855345E-5	0.0	0.0	1.135104379942138E-4	0.0
14-15	2.837760949855345E-5	0.0	0.0	1.4188804749276727E-4	0.0
16-17	2.837760949855345E-5	0.0	0.0	1.5607685224204397E-4	0.0
18-19	4.2566414247830176E-5	0.0	0.0	1.9864326648987417E-4	0.0
20-21	5.67552189971069E-5	0.0	0.0	2.270208759884276E-4	0.0
22-23	5.67552189971069E-5	0.0	0.0	4.682305567261319E-4	0.0
24-25	5.67552189971069E-5	0.0	0.0	0.001092537965694308	0.0
26-27	5.67552189971069E-5	0.0	0.0	0.0020289990791465718	0.0
28-29	5.67552189971069E-5	0.0	0.0	0.004398529472275785	0.0
30-31	5.67552189971069E-5	0.0	0.0	0.01023012822422852	0.0
32-33	5.67552189971069E-5	0.0	0.0	0.021382528757160027	0.0
34-35	5.67552189971069E-5	0.0	0.0	0.03672062669112817	0.0
36-37	5.67552189971069E-5	0.0	0.0	0.05913893819498539	0.0
38-39	5.67552189971069E-5	0.0	0.0	0.08982932286767095	0.0
40-41	5.67552189971069E-5	0.0	0.0	0.12236425215776249	0.0
42-43	5.67552189971069E-5	0.0	0.0	0.15321071368269007	0.0
44-45	5.67552189971069E-5	0.0	0.0	0.18973269710732837	0.0
46-47	5.67552189971069E-5	0.0	0.0	0.23594563417572267	0.0
48-49	5.67552189971069E-5	0.0	0.0	0.2839747382520244	0.0
50-51	5.67552189971069E-5	0.0	0.0	0.33404703021222193	0.0
52-53	8.513282849566035E-5	0.0	0.0	0.38311191703522085	0.0
54-55	8.513282849566035E-5	0.0	0.0	0.43755436085819566	0.0
56-57	8.513282849566035E-5	0.0	0.0	0.503560680551831	0.0
58-59	9.932163324493707E-5	0.0	0.0	0.5810599320923804	0.0
60-61	1.135104379942138E-4	0.0	0.0	0.6629009578862086	0.0
62-63	1.135104379942138E-4	0.0	0.0	0.7467284163449355	0.0
64-65	1.135104379942138E-4	0.0	0.0	0.8359334318036382	0.0
66-67	1.135104379942138E-4	0.0	0.0	0.9349996665630884	0.0
68-69	1.135104379942138E-4	0.0	0.0	1.0535329414385464	0.0
70-71	1.135104379942138E-4	0.0	0.0	1.1881137544854359	0.0
72-73	1.135104379942138E-4	0.0	0.0	1.3314348712578803	0.0
74-75	1.135104379942138E-4	0.0	0.0	1.4810841949485019	0.0
76-77	1.135104379942138E-4	0.0	0.0	1.6391474798554444	0.0
78-79	1.135104379942138E-4	0.0	0.0	1.8208209358651837	0.0
80-81	1.135104379942138E-4	0.0	0.0	2.0277220867191366	0.0
82-83	1.135104379942138E-4	0.0	0.0	2.252940984504406	0.0
84-85	1.135104379942138E-4	0.0	0.0	2.479919294078586	0.0
86-87	1.135104379942138E-4	0.0	0.0	2.719142542151392	0.0
88	1.135104379942138E-4	0.0	0.0	2.9154588446623846	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5660	0.0	46.916473	1
GTATCAA	10055	0.0	35.851444	1
TCAACGC	11625	0.0	29.230663	4
ATCAACG	11685	0.0	29.201239	3
TATCAAC	13285	0.0	26.745674	2
CAACGCA	13170	0.0	25.801554	5
AACGCAG	13560	0.0	25.059471	6
GTACATG	20645	0.0	24.677872	1
TACATGG	20085	0.0	23.98531	2
ACGCAGA	14290	0.0	23.384642	7
CGCAGAG	14295	0.0	23.212072	8
ACATGGG	21360	0.0	21.607452	3
GCAGAGT	15270	0.0	20.62192	9
CATGGGG	11175	0.0	19.598902	4
TCTGTCG	770	0.0	18.92188	8
CATGGGT	3065	0.0	18.24777	4
CAGAGTA	15140	0.0	17.710186	10-11
GTGGTAT	2980	0.0	17.348814	1
TATACTG	1365	0.0	17.215937	5
CATGGGA	6950	0.0	17.109175	4
>>END_MODULE