FastQCFastQC Report
Wed 25 May 2016
SRR2431354_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431354_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3523905
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265060.7521769173686578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208770.5924393535013004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144410.4098010587686104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43590.12369799980419449No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42110.11949811359840859No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41610.11807923312348091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98650.062.938361
GTATCAA198450.044.715751
ATCAACG264250.033.4730873
TCAACGC266500.033.154274
TATCAAC274500.032.274552
CAACGCA279300.031.6344015
AACGCAG288400.030.6851146
TGGTATC32850.028.0421872
ACGCAGA318150.027.6680497
CGCAGAG320000.027.434668
GTGGTAT34450.026.8773631
GCAGAGT338950.025.665139
GTTGCGA1351.4592084E-524.3689586
GTACATG193850.024.0764871
TACATGG190950.023.924222
TATAACG2602.7284841E-1123.4996382
ACATGGG194500.022.5209883
AGAGTAC334800.021.68781310-11
AGTACTT234800.021.66718512-13
CAGAGTA337000.021.61595710-11
Produced by FastQC (version 0.11.5)