##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431354_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3523905
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71080009251101	34.0	31.0	34.0	31.0	34.0
2	32.83963443963444	34.0	31.0	34.0	31.0	34.0
3	32.90921094637909	34.0	31.0	34.0	31.0	34.0
4	36.31005603158995	37.0	37.0	37.0	35.0	37.0
5	36.26688659314028	37.0	37.0	37.0	35.0	37.0
6	36.30713058382675	37.0	37.0	37.0	35.0	37.0
7	36.17680442577198	37.0	37.0	37.0	35.0	37.0
8	36.235737626298096	37.0	37.0	37.0	35.0	37.0
9	38.045539820171086	39.0	39.0	39.0	35.0	39.0
10-11	38.03880127301956	39.0	39.0	39.0	35.0	39.0
12-13	38.01321077043791	39.0	39.0	39.0	35.0	39.0
14-15	39.46429954837035	41.0	39.0	41.0	36.0	41.0
16-17	39.471439213032134	41.0	39.0	41.0	36.0	41.0
18-19	39.554794326180755	41.0	40.0	41.0	36.5	41.0
20-21	39.56180941313684	41.0	40.0	41.0	37.0	41.0
22-23	39.48202718291214	41.0	39.5	41.0	36.0	41.0
24-25	39.44398586227494	41.0	39.0	41.0	36.0	41.0
26-27	39.342898006614824	41.0	39.0	41.0	36.0	41.0
28-29	39.24885602761709	41.0	39.0	41.0	35.0	41.0
30-31	39.05213548605879	41.0	39.0	41.0	35.0	41.0
32-33	38.80790387368558	41.0	38.0	41.0	35.0	41.0
34-35	38.68864171991015	41.0	38.0	41.0	35.0	41.0
36-37	38.561743009530616	41.0	38.0	41.0	35.0	41.0
38-39	38.42365855492699	41.0	38.0	41.0	35.0	41.0
40-41	38.2910838969836	40.0	38.0	41.0	35.0	41.0
42-43	38.111748897884596	40.0	38.0	41.0	34.0	41.0
44-45	37.837899432589694	40.0	38.0	41.0	33.5	41.0
46-47	37.42124617434352	40.0	37.5	41.0	33.0	41.0
48-49	37.051330980829505	40.0	37.0	41.0	32.0	41.0
50-51	36.83932058327339	40.0	37.0	41.0	31.0	41.0
52-53	36.675767082256755	40.0	36.5	41.0	31.0	41.0
54-55	36.49021979877437	40.0	36.0	41.0	31.0	41.0
56-57	36.29025002092849	40.0	35.0	41.0	31.0	41.0
58-59	36.067291257851736	39.0	35.0	41.0	30.0	41.0
60-61	35.83810091361714	39.0	35.0	41.0	30.0	41.0
62-63	35.57783396544458	39.0	35.0	41.0	30.0	41.0
64-65	35.27988907192447	38.0	35.0	41.0	29.0	41.0
66-67	34.97237936323482	37.0	35.0	40.0	29.0	41.0
68-69	34.68536325468479	37.0	35.0	40.0	29.0	41.0
70-71	34.42490078478279	36.0	35.0	39.0	29.0	41.0
72-73	34.169622052807895	36.0	35.0	39.0	29.0	41.0
74-75	33.87898297485318	35.0	35.0	38.5	29.0	40.5
76-77	33.583999852436435	35.0	35.0	37.0	29.0	39.5
78-79	33.2929514274647	35.0	34.0	37.0	29.0	39.0
80-81	33.02468738515936	35.0	34.0	36.5	28.0	39.0
82-83	32.75274815864786	35.0	34.0	36.0	27.5	38.0
84-85	32.530173202739576	35.0	34.0	36.0	27.0	37.0
86-87	32.347684174232846	35.0	34.0	35.5	27.0	37.0
88-89	32.16466590330897	35.0	34.0	35.0	27.0	36.5
90-91	31.994928921182613	35.0	34.0	35.0	26.0	36.0
92-93	31.874986697995546	35.0	34.0	35.0	26.0	36.0
94-95	31.762421801949827	35.0	34.0	35.0	26.0	36.0
96-97	31.64277016548403	35.0	34.0	35.0	25.0	36.0
98-99	31.53720148528408	35.0	34.0	35.0	25.0	35.0
100	31.43721014045498	35.0	34.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23507952242372454
1101	2	0.22123057086633935
1101	3	0.23016318104138378
1101	4	0.16249361456245737
1101	5	0.16117477413005332
1101	6	0.21453523287245702
1101	7	0.18589048088644233
1101	8	0.18319057132621452
1101	9	0.2495197404871874
1101	10-11	0.22580124848663985
1101	12-13	0.25311399123859957
1101	14-15	0.3066599529696816
1101	16-17	0.35458647344469085
1101	18-19	0.3017654882395888
1101	20-21	0.30856311135404013
1101	22-23	0.3292896588329768
1101	24-25	0.29431268464657734
1101	26-27	0.3140579514852533
1101	28-29	0.3104125462915803
1101	30-31	0.32804257832188455
1101	32-33	0.37981517975252643
1101	34-35	0.4253227485541089
1101	36-37	0.3867147930713557
1101	38-39	0.41409536022413107
1101	40-41	0.3785544171757351
1101	42-43	0.39048835099640655
1101	44-45	0.47063220655049776
1101	46-47	0.473983026990183
1101	48-49	0.5009389942024782
1101	50-51	0.5244669568176477
1101	52-53	0.541948999172547
1101	54-55	0.4989364534784997
1101	56-57	0.47183502189052007
1101	58-59	0.501330914771998
1101	60-61	0.4914222708334819
1101	62-63	0.42238233020939475
1101	64-65	0.49899321486544324
1101	66-67	0.50563667803155
1101	68-69	0.46780354410088165
1101	70-71	0.479462462305996
1101	72-73	0.4259661223452511
1101	74-75	0.42508322033305035
1101	76-77	0.38629416191056976
1101	78-79	0.3949425933653643
1101	80-81	0.3791544667704443
1101	82-83	0.41531213445342274
1101	84-85	0.3729877669333206
1101	86-87	0.3751549178118765
1101	88-89	0.4599467710670737
1101	90-91	0.39201413277851316
1101	92-93	0.4000274025966384
1101	94-95	0.38059710370581
1101	96-97	0.4242865844044523
1101	98-99	0.43758744396369664
1101	100	0.43007040599803403
1102	1	0.1294652369795486
1102	2	0.10641073611940755
1102	3	0.1414108088746744
1102	4	0.07462965428051405
1102	5	0.08104007188113371
1102	6	0.08661063866596663
1102	7	0.11924391606248008
1102	8	0.14811857787025673
1102	9	0.18496247362129026
1102	10-11	0.1794041803702484
1102	12-13	0.1562345535329186
1102	14-15	0.27171533752688504
1102	16-17	0.2677381786680826
1102	18-19	0.25776098721807017
1102	20-21	0.2706853186159819
1102	22-23	0.2592208050460272
1102	24-25	0.2751523694656015
1102	26-27	0.24283212220960593
1102	28-29	0.23196634907260005
1102	30-31	0.23219224052137122
1102	32-33	0.3080140347436071
1102	34-35	0.2525400713668162
1102	36-37	0.19009451488972218
1102	38-39	0.23012605733016045
1102	40-41	0.2260560666214957
1102	42-43	0.22340583989809915
1102	44-45	0.2497904518387557
1102	46-47	0.26524268109916704
1102	48-49	0.41609690313315895
1102	50-51	0.41088296246208245
1102	52-53	0.37556656957626444
1102	54-55	0.3474104798079338
1102	56-57	0.278917164786769
1102	58-59	0.3502390712439478
1102	60-61	0.3354605817612182
1102	62-63	0.2671473426536366
1102	64-65	0.30331620570119355
1102	66-67	0.3713605981297121
1102	68-69	0.3300120051854165
1102	70-71	0.24749267079793213
1102	72-73	0.3130665360252536
1102	74-75	0.3138901759686803
1102	76-77	0.2785326115609337
1102	78-79	0.2589404418711112
1102	80-81	0.24008019145680493
1102	82-83	0.29024906740979617
1102	84-85	0.2376778602078673
1102	86-87	0.24471933476390006
1102	88-89	0.26108717746264887
1102	90-91	0.2854687687908708
1102	92-93	0.3178524130704936
1102	94-95	0.2257723629150874
1102	96-97	0.1981101290360172
1102	98-99	0.21030389453555287
1102	100	0.26670387477001967
1103	1	0.12292087785016292
1103	2	0.14367122649176167
1103	3	0.12421639883852009
1103	4	0.07884004792671817
1103	5	0.1020525717744647
1103	6	0.11777085024908018
1103	7	0.11268216132480546
1103	8	0.14665759293747982
1103	9	0.1257024466941985
1103	10-11	0.165215928379439
1103	12-13	0.14375000444349695
1103	14-15	0.2195488622119086
1103	16-17	0.22762973182019408
1103	18-19	0.19721877267179622
1103	20-21	0.18837241822979678
1103	22-23	0.23187609859527214
1103	24-25	0.19664434812086284
1103	26-27	0.20559899709842
1103	28-29	0.2137339463714838
1103	30-31	0.2759758788686355
1103	32-33	0.25215591595063813
1103	34-35	0.22234145947071937
1103	36-37	0.22799289893617924
1103	38-39	0.22554149399603318
1103	40-41	0.19386102148153128
1103	42-43	0.24117799747211421
1103	44-45	0.19017246103808105
1103	46-47	0.19604200765937208
1103	48-49	0.3031502134517794
1103	50-51	0.28609864338855573
1103	52-53	0.19984782735095052
1103	54-55	0.25517469776374213
1103	56-57	0.22820666906444131
1103	58-59	0.22177197060608478
1103	60-61	0.13477114128932044
1103	62-63	0.11980707372408972
1103	64-65	0.21654769103012939
1103	66-67	0.19697516363665102
1103	68-69	0.138069765825783
1103	70-71	0.17655834447076302
1103	72-73	0.1746946468031112
1103	74-75	0.16668796416632148
1103	76-77	0.19096213473089563
1103	78-79	0.18704788010193596
1103	80-81	0.20241090428944375
1103	82-83	0.20761586628309203
1103	84-85	0.18066178469648264
1103	86-87	0.14691219888844387
1103	88-89	0.13798727842281977
1103	90-91	0.19442089345107405
1103	92-93	0.22909665528825585
1103	94-95	0.18626792112702972
1103	96-97	0.13940421787703272
1103	98-99	0.16706189963345253
1103	100	0.2145314915126022
1104	1	0.11913254466571743
1104	2	0.14614254440036234
1104	3	0.14512816391975036
1104	4	0.051291521273753915
1104	5	0.07612734301962831
1104	6	0.06864152241181642
1104	7	0.04081996298128132
1104	8	0.1020577384217276
1104	9	0.13575617377086502
1104	10-11	0.1490612747978517
1104	12-13	0.19566076134442056
1104	14-15	0.20489531252393078
1104	16-17	0.22969602286526225
1104	18-19	0.21856459765680114
1104	20-21	0.18772268431282413
1104	22-23	0.18030671920043773
1104	24-25	0.17979944198372522
1104	26-27	0.21863371550983857
1104	28-29	0.14351259336800126
1104	30-31	0.19740114590879898
1104	32-33	0.20738664885942626
1104	34-35	0.255942577402287
1104	36-37	0.25373308194004807
1104	38-39	0.2207141927287921
1104	40-41	0.1610849523637512
1104	42-43	0.19882724644159566
1104	44-45	0.1389602518470454
1104	46-47	0.22238662027566392
1104	48-49	0.1525821549007489
1104	50-51	0.09324779026383112
1104	52-53	0.10621372022860243
1104	54-55	0.12144261210670493
1104	56-57	0.14757808779106796
1104	58-59	0.12731903603571482
1104	60-61	0.1059957294029985
1104	62-63	0.062084604065212545
1104	64-65	0.1038175004968167
1104	66-67	0.11199481677157763
1104	68-69	0.13322252428364578
1104	70-71	0.11970925885200501
1104	72-73	0.10780252209504226
1104	74-75	0.13316150262392767
1104	76-77	0.07589462493623245
1104	78-79	0.1279718645836212
1104	80-81	0.11053840081185484
1104	82-83	0.19788708592601978
1104	84-85	0.13898214355762661
1104	86-87	0.18469917932463886
1104	88-89	0.19506320460870796
1104	90-91	0.22499124912177138
1104	92-93	0.25623584797831356
1104	94-95	0.23808902655206055
1104	96-97	0.24581461744823585
1104	98-99	0.2032992814275012
1104	100	0.20491740761669064
1105	1	-0.06441095493961768
1105	2	0.03219857376588209
1105	3	0.037076228785728915
1105	4	-0.11796660782930246
1105	5	-0.08811936330589276
1105	6	-0.05884533818537818
1105	7	-0.3790919276167486
1105	8	-0.3506938354188307
1105	9	-0.3202560254988498
1105	10-11	-0.3448415770461608
1105	12-13	-0.22250546573782515
1105	14-15	-0.17885634795004535
1105	16-17	-0.11910562830365024
1105	18-19	-0.11184894860223693
1105	20-21	-0.08983773447956622
1105	22-23	-0.12140308770328545
1105	24-25	-0.10824899572684643
1105	26-27	-0.1330318923038618
1105	28-29	-0.22268464848043124
1105	30-31	-0.17892418506296792
1105	32-33	-0.21281626587402513
1105	34-35	-0.19059465250999352
1105	36-37	-0.21933980564827493
1105	38-39	-0.28677310103552855
1105	40-41	-0.3057738319446912
1105	42-43	-0.3520117341075917
1105	44-45	-0.3692225960182398
1105	46-47	-0.4184610396592632
1105	48-49	-0.503335228898159
1105	50-51	-0.47115269039124286
1105	52-53	-0.43835267947876844
1105	54-55	-0.4141293128880008
1105	56-57	-0.39327213906015146
1105	58-59	-0.38971694571351634
1105	60-61	-0.3734934061237638
1105	62-63	-0.39820993353689715
1105	64-65	-0.3254207189616949
1105	66-67	-0.3383984829351476
1105	68-69	-0.27826089664570475
1105	70-71	-0.33777169783769523
1105	72-73	-0.2732322981245616
1105	74-75	-0.3437813321500798
1105	76-77	-0.3331662687356811
1105	78-79	-0.3193858159508167
1105	80-81	-0.31351272253488816
1105	82-83	-0.22496328210561245
1105	84-85	-0.2126013229344892
1105	86-87	-0.25683737677210416
1105	88-89	-0.25660160104363783
1105	90-91	-0.2276087964409932
1105	92-93	-0.19946142274793388
1105	94-95	-0.20544378826417997
1105	96-97	-0.2388470148538424
1105	98-99	-0.19936140753283027
1105	100	-0.2031093396511494
1106	1	0.059732572745417656
1106	2	0.09644323118300946
1106	3	0.08501566962343787
1106	4	0.06506461390166152
1106	5	0.09626165758245975
1106	6	0.05189344411625285
1106	7	0.08835743183036726
1106	8	0.10951289865150926
1106	9	0.09639837989925581
1106	10-11	0.13787487824229316
1106	12-13	0.17120348928723672
1106	14-15	0.19911133316251295
1106	16-17	0.2117371537751609
1106	18-19	0.1720460416553493
1106	20-21	0.13660549251677878
1106	22-23	0.13999421554422042
1106	24-25	0.10464295615812347
1106	26-27	0.09796647164934313
1106	28-29	0.13007484840186834
1106	30-31	0.15550638043279008
1106	32-33	0.09963650553596892
1106	34-35	0.0959571729961155
1106	36-37	0.08175267338041436
1106	38-39	0.0770534773984437
1106	40-41	0.1790626875019825
1106	42-43	0.15861916416808697
1106	44-45	0.12984093067378666
1106	46-47	0.1565166175846926
1106	48-49	0.21960430276376997
1106	50-51	0.236030429989583
1106	52-53	0.24230871151219446
1106	54-55	0.23810659755206132
1106	56-57	0.22300381698577354
1106	58-59	0.22715860150236722
1106	60-61	0.20367871544505078
1106	62-63	0.23316880221430125
1106	64-65	0.25595989382320283
1106	66-67	0.2735579492960625
1106	68-69	0.27711401964464955
1106	70-71	0.24933129439622803
1106	72-73	0.2750488688091295
1106	74-75	0.2420283989981371
1106	76-77	0.24217968068613516
1106	78-79	0.22767293278218403
1106	80-81	0.20360900370641133
1106	82-83	0.26929405437144993
1106	84-85	0.24551418366479538
1106	86-87	0.19959545993444294
1106	88-89	0.25718778487570404
1106	90-91	0.2686496244771561
1106	92-93	0.2611933774327504
1106	94-95	0.3156953199147239
1106	96-97	0.28080704177929405
1106	98-99	0.2720005075743863
1106	100	0.3284800779961152
1107	1	-0.04435763653150815
1107	2	0.025943279193505475
1107	3	0.032855522890351097
1107	4	-0.06324471960961375
1107	5	-0.08449093539108077
1107	6	-0.045524662729611975
1107	7	-0.04633078747939834
1107	8	-0.029850783281403892
1107	9	0.0038786996654209815
1107	10-11	0.0407527004862871
1107	12-13	0.05033974930773155
1107	14-15	0.05891960583173983
1107	16-17	0.046823480440728815
1107	18-19	0.0488928181982331
1107	20-21	0.04232617343035372
1107	22-23	0.07481722363765186
1107	24-25	0.05339446631871425
1107	26-27	0.04838716021205158
1107	28-29	0.03649130851491833
1107	30-31	0.03452329233272167
1107	32-33	0.0739381088152129
1107	34-35	0.060187761431173215
1107	36-37	0.046258372795662694
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2216	48-49	-0.32530347694532225
2216	50-51	-0.2872176571911922
2216	52-53	-0.4132890881662732
2216	54-55	-0.389737637837996
2216	56-57	-0.37539407512516476
2216	58-59	-0.43130825403432027
2216	60-61	-0.40009485477491324
2216	62-63	-0.45319186334554473
2216	64-65	-0.3785843401139459
2216	66-67	-0.2732855674823469
2216	68-69	-0.3407178810393461
2216	70-71	-0.35200202886670695
2216	72-73	-0.47814206037925544
2216	74-75	-0.41378895083773415
2216	76-77	-0.3602963642469277
2216	78-79	-0.3133212558266223
2216	80-81	-0.34811007748540845
2216	82-83	-0.405730402870347
2216	84-85	-0.4052540170317229
2216	86-87	-0.3558615952937245
2216	88-89	-0.2766857544642498
2216	90-91	-0.3287225567421288
2216	92-93	-0.39845810052955954
2216	94-95	-0.4098898579358945
2216	96-97	-0.5589961794735316
2216	98-99	-0.5882416052822386
2216	100	-0.5510099187498376
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45.0
3	387.0
4	261.0
5	391.0
6	526.0
7	850.0
8	1508.0
9	2312.0
10	3070.0
11	4389.0
12	6991.0
13	10663.0
14	11125.0
15	15021.0
16	20496.0
17	33877.0
18	42510.0
19	20487.0
20	10902.0
21	9076.0
22	8951.0
23	9100.0
24	10123.0
25	12010.0
26	15155.0
27	19398.0
28	23744.0
29	29831.0
30	37649.0
31	47768.0
32	59397.0
33	75869.0
34	106673.0
35	178850.0
36	325963.0
37	747478.0
38	1122365.0
39	494074.0
40	4620.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.40108888389401	20.782760170824904	14.939289500988759	23.876861444292327
2	14.542361386019204	21.698541816535915	39.349641945512154	24.409454851932725
3	15.866012881783234	28.590895688884814	30.893095161002428	24.649996268329524
4	10.900577898203192	18.783195660144546	39.25544667070019	31.060779770952074
5	12.02237290732866	38.40063792866153	34.846881513548176	14.730107650461633
6	27.891813201547716	39.41618743978626	18.209259330203285	14.482740028462743
7	24.964974935476413	34.02512269768907	23.207549579230996	17.802352787603525
8	25.52409897542641	33.762062257637474	20.540281307242957	20.173557459693154
9	26.729835225410447	15.174614525646973	21.52804913866861	36.56750111027397
10-11	23.806260384431475	26.790946407465583	28.39869406241088	21.004099145692066
12-13	25.39993274506549	25.442030928756594	28.259516076625218	20.8985202495527
14-15	22.87317620650954	26.050730652500565	27.526337968815845	23.54975517217405
16-17	20.500581031554482	28.853700653110682	27.977712225499836	22.668006089835
18-19	20.40199721615651	26.66933416195953	32.16123873940983	20.767429882474133
20-21	22.289420401514796	25.663376282845306	31.74052648978903	20.306676825850868
22-23	21.700677501910878	25.743128745227413	31.853543579467246	20.70265017339446
24-25	20.955389656779662	26.080105791803266	31.97741708233982	20.987087469077252
26-27	20.845799451457694	26.15482627838309	31.99213368200154	21.00724058815767
28-29	20.734476062910904	25.931571020234134	32.5327462897707	20.801206627084266
30-31	21.24358264542433	26.32367560075747	31.836151610613754	20.59659014320445
32-33	20.734931785584756	26.208573158874852	32.074836920523694	20.981658135016694
34-35	21.142036577976874	25.753787561671594	32.048681714275176	21.05549414607636
36-37	21.36473223793889	26.17456073473925	31.322608122276428	21.138098905045428
38-39	21.168918091534845	26.263069854451388	31.496687317163218	21.071324736850546
40-41	21.101899096992437	25.989094097815414	31.529039837901347	21.379966967290805
42-43	20.980298871884138	26.270057943159035	31.469660824581506	21.279982360375318
44-45	21.32013388632149	26.270423126747893	30.88603913010782	21.523403856822796
46-47	21.574108430666282	26.2811943733598	30.28536887667975	21.859328319294168
48-49	21.570926093121397	26.513673586288967	29.677981519768537	22.2374188008211
50-51	21.95166213896511	26.637542378917683	29.04338222002388	22.367413262093333
52-53	22.00191889449883	26.929422594940156	28.808318841829973	22.260339668731042
54-55	22.02999731971691	27.23164782924738	28.800940099716748	21.937414751318965
56-57	22.016517339943487	26.727641252627617	28.835010347281294	22.42083106014761
58-59	21.953025311755393	26.03700782362833	29.36178381960889	22.648183045007393
60-61	22.174903920072396	26.63396889062056	28.977402523178224	22.213724666128815
62-63	21.827120765173863	26.49015793558566	29.264466550602243	22.41825474863823
64-65	21.95842396432367	26.56559981043757	29.19943925843631	22.276536966802453
66-67	21.991011874297914	26.410994271499398	29.164755298256477	22.433238555946208
68-69	22.37072656470039	26.566857105560565	28.97416269436154	22.088253635377505
70-71	22.269836175271944	26.382325239008615	28.925994380541383	22.42184420517806
72-73	22.1077799552882	26.457120544214906	28.816479572927967	22.61861992756893
74-75	22.192593799191844	26.643644374545772	28.661207078319023	22.50255474794336
76-77	22.385397069805276	26.510051163548976	28.51492049068992	22.589631275955828
78-79	22.3778358075113	26.528166240240825	28.69270777759868	22.40129017464919
80-81	22.00651726282907	26.769857134424864	28.812113493339147	22.411512109406925
82-83	22.024827922828834	26.721956493669573	28.892136594723677	22.361078988777916
84-85	21.847617493588356	26.44413622835738	28.792894160881705	22.915352117172564
86-87	22.069832406990674	26.719943902966435	28.604364944007965	22.605858746034926
88-89	21.71167898747109	26.67731316599742	28.64740269875278	22.963605147778708
90-91	21.586629476188058	26.517016516920034	28.712802308617213	23.183551698274695
92-93	21.574813964720978	26.779090945724978	28.78965115440914	22.856443935144906
94-95	21.48277835314042	26.30860238808842	29.007159611273366	23.201459647497796
96-97	21.5395831346855	26.665931669686117	28.803751265670474	22.99073392995791
98-99	21.231945575042115	26.58788767715305	28.829202475889478	23.35096427191536
100	21.338148638785118	26.395354512958303	28.981821711102107	23.284675137154476
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3532.0
1	2149.0
2	1821.5
3	2641.0
4	1515.0
5	1046.5
6	2841.5
7	7500.0
8	12764.0
9	13374.0
10	8176.5
11	3492.5
12	2652.0
13	2721.5
14	2970.5
15	3204.5
16	3548.5
17	4119.0
18	4651.5
19	5260.0
20	6133.0
21	7248.0
22	8670.0
23	10641.5
24	13609.0
25	17139.0
26	21366.0
27	27206.5
28	33941.5
29	41033.5
30	47576.5
31	53974.0
32	60881.5
33	66516.0
34	72362.0
35	80530.5
36	87122.0
37	93927.0
38	102352.0
39	106688.0
40	111638.0
41	118459.0
42	122450.5
43	131789.0
44	137736.0
45	134335.5
46	133551.5
47	135309.0
48	134381.5
49	133000.0
50	134567.5
51	127948.0
52	114979.5
53	104867.0
54	97596.0
55	91863.5
56	81306.0
57	69705.5
58	63930.5
59	60157.0
60	52499.5
61	42249.0
62	34458.5
63	27502.5
64	20787.5
65	15871.0
66	12164.5
67	9107.5
68	6558.0
69	4701.5
70	3304.0
71	2376.5
72	1696.0
73	1158.5
74	916.5
75	694.0
76	432.5
77	288.5
78	189.5
79	106.0
80	52.5
81	24.5
82	12.0
83	6.5
84	4.5
85	3.0
86	2.5
87	2.0
88	1.5
89	2.0
90	2.5
91	2.0
92	1.5
93	1.5
94	3.5
95	7.0
96	6.0
97	3.0
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008428150021070375
2	0.0
3	3.972865329797483E-4
4	0.0028661385593538985
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.932163324493707E-5
24-25	7.094402374638363E-5
26-27	5.675521899710691E-4
28-29	9.222723087029871E-4
30-31	0.001759411788910314
32-33	0.004625550348264213
34-35	0.00567552189971069
36-37	0.004299207839030848
38-39	0.002823572145106068
40-41	0.0035472011873191817
42-43	5.817409947203458E-4
44-45	5.675521899710691E-4
46-47	0.003760033258558332
48-49	0.0024262856121263204
50-51	0.001191859598939245
52-53	2.837760949855345E-5
54-55	6.668738232160061E-4
56-57	9.222723087029871E-4
58-59	8.087618707087734E-4
60-61	4.540417519768552E-4
62-63	0.0
64-65	0.0
66-67	9.506499182015407E-4
68-69	7.8038426121022E-4
70-71	0.0022134535408871695
72-73	8.513282849566035E-5
74-75	0.0020999431028929554
76-77	2.695872902362578E-4
78-79	0.0011634819894406916
80-81	9.364611134522638E-4
82-83	0.0014188804749276725
84-85	0.0012060484036885218
86-87	3.1215370448408795E-4
88-89	0.00156076852242044
90-91	0.0018161670079074209
92-93	0.0031499146543394335
94-95	0.0011492931846914147
96-97	0.0022985863693828295
98-99	0.002284397564633553
100	0.004001242939296036
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3523905.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.82287080798771
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.32744549799283	32.6842832011831
2	15.686657489475492	14.376153590987768
3	5.56378579860527	7.648459135584182
4	2.515953437341097	4.611528372727749
5	1.394477230830105	3.194947499650419
6	0.831295056404905	2.2855395583776494
7	0.500438708453256	1.6052076799338844
8	0.3492794501218217	1.2803989695053781
9	0.27465405005181925	1.1326893347173594
>10	1.3143316255247668	10.91075647611601
>50	0.12274897277789304	3.9602573736018365
>100	0.10732659905418121	9.806110927897935
>500	0.008626981755507898	2.637790451639287
>1k	0.0027929077604163816	2.107615899420015
>5k	0.0	0.0
>10k+	1.8619385069442544E-4	1.7582615286574055
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26506	0.7521769173686578	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20877	0.5924393535013004	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14441	0.4098010587686104	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4359	0.12369799980419449	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4211	0.11949811359840859	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4161	0.11807923312348091	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.837760949855345E-5	0.0
4	0.0	0.0	0.0	2.837760949855345E-5	0.0
5	2.837760949855345E-5	0.0	0.0	2.837760949855345E-5	0.0
6	2.837760949855345E-5	0.0	0.0	2.837760949855345E-5	0.0
7	2.837760949855345E-5	0.0	0.0	2.837760949855345E-5	0.0
8	2.837760949855345E-5	0.0	0.0	2.837760949855345E-5	0.0
9	5.67552189971069E-5	0.0	0.0	2.837760949855345E-5	0.0
10-11	5.67552189971069E-5	0.0	0.0	2.837760949855345E-5	0.0
12-13	5.67552189971069E-5	0.0	0.0	7.094402374638363E-5	0.0
14-15	5.67552189971069E-5	0.0	0.0	8.513282849566035E-5	0.0
16-17	5.67552189971069E-5	0.0	0.0	9.932163324493707E-5	0.0
18-19	7.094402374638363E-5	0.0	0.0	1.4188804749276727E-4	0.0
20-21	8.513282849566035E-5	0.0	0.0	1.5607685224204397E-4	0.0
22-23	8.513282849566035E-5	0.0	0.0	4.1147533772902504E-4	0.0
24-25	8.513282849566035E-5	0.0	0.0	0.0010783491609450313	0.0
26-27	8.513282849566035E-5	0.0	0.0	0.002014810274397295	0.0
28-29	8.513282849566035E-5	0.0	0.0	0.004398529472275785	0.0
30-31	8.513282849566035E-5	0.0	0.0	0.010343638662222734	0.0
32-33	8.513282849566035E-5	0.0	0.0	0.021510227999903514	0.0
34-35	8.513282849566035E-5	0.0	0.0	0.03679157071487455	0.0
36-37	8.513282849566035E-5	0.0	0.0	0.05936595907097382	0.0
38-39	8.513282849566035E-5	0.0	0.0	0.09016985418165359	0.0
40-41	8.513282849566035E-5	0.0	0.0	0.12288923793348572	0.0
42-43	8.513282849566035E-5	0.0	0.0	0.15413298599139308	0.0
44-45	8.513282849566035E-5	0.0	0.0	0.19078266865877486	0.0
46-47	8.513282849566035E-5	0.0	0.0	0.2370239833366677	0.0
48-49	8.513282849566035E-5	0.0	0.0	0.2854787515554477	0.0
50-51	8.513282849566035E-5	0.0	0.0	0.33596251885337436	0.0
52-53	1.4188804749276727E-4	0.0	0.0	0.3857226571090878	0.0
54-55	1.4188804749276727E-4	0.0	0.0	0.4407752195362815	0.0
56-57	1.4188804749276727E-4	0.0	0.0	0.5075477346863777	0.0
58-59	1.4188804749276727E-4	0.0	0.0	0.5862246570211171	0.0
60-61	1.4188804749276727E-4	0.0	0.0	0.6688886334904034	0.0
62-63	1.702656569913207E-4	0.0	0.0	0.7534822874055912	0.0
64-65	1.702656569913207E-4	0.0	0.0	0.8437940296347376	0.0
66-67	1.702656569913207E-4	0.0	0.0	0.9441514456263719	0.0
68-69	1.702656569913207E-4	0.0	0.0	1.0647279083857255	0.0
70-71	1.702656569913207E-4	0.0	0.0	1.2010255668072776	0.0
72-73	1.702656569913207E-4	0.0	0.0	1.3467162139728512	0.0
74-75	2.5539848548698107E-4	0.0	0.0	1.4987634456661005	0.0
76-77	2.5539848548698107E-4	0.0	0.0	1.6595084146706567	0.0
78-79	2.5539848548698107E-4	0.0	0.0	1.8436649115115191	0.0
80-81	2.5539848548698107E-4	0.0	0.0	2.0540565083337947	0.0
82-83	2.5539848548698107E-4	0.0	0.0	2.2821841110926657	0.0
84-85	2.5539848548698107E-4	0.0	0.0	2.5126386778304184	0.0
86-87	2.5539848548698107E-4	0.0	0.0	2.7569273291987155	0.0
88	2.5539848548698107E-4	0.0	0.0	2.956407735168797	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9865	0.0	62.93836	1
GTATCAA	19845	0.0	44.71575	1
ATCAACG	26425	0.0	33.473087	3
TCAACGC	26650	0.0	33.15427	4
TATCAAC	27450	0.0	32.27455	2
CAACGCA	27930	0.0	31.634401	5
AACGCAG	28840	0.0	30.685114	6
TGGTATC	3285	0.0	28.042187	2
ACGCAGA	31815	0.0	27.668049	7
CGCAGAG	32000	0.0	27.43466	8
GTGGTAT	3445	0.0	26.877363	1
GCAGAGT	33895	0.0	25.66513	9
GTTGCGA	135	1.4592084E-5	24.368958	6
GTACATG	19385	0.0	24.076487	1
TACATGG	19095	0.0	23.92422	2
TATAACG	260	2.7284841E-11	23.499638	2
ACATGGG	19450	0.0	22.520988	3
AGAGTAC	33480	0.0	21.687813	10-11
AGTACTT	23480	0.0	21.667185	12-13
CAGAGTA	33700	0.0	21.615957	10-11
>>END_MODULE