FastQCFastQC Report
Wed 25 May 2016
SRR2431353_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431353_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5061971
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA189740.37483422959159585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55000.040.9324231
GTATCAA94500.036.605021
TCAACGC100350.032.64454
ATCAACG101300.032.570343
CAACGCA108000.030.3756985
TATCAAC118700.028.3106582
AACGCAG117550.027.9079156
GTACATG212700.026.8916661
TACATGG207050.026.5813052
ACATGGG211100.025.0249213
CGCAGAG130800.024.4700018
ACGCAGA133250.024.3022587
TACCTGG37500.023.6878242
GTACCTG43100.021.1552941
CATGGGG120300.020.8626864
GCAGAGT153000.020.1822039
CATGGGT48450.019.4983084
CATGGGA69950.018.6789174
ACCTGGG48650.017.679213
AGAGTAC135250.017.35772710-11
Produced by FastQC (version 0.11.5)