##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431353_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5061971
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.307056480568537	33.0	31.0	34.0	28.0	34.0
2	31.400216634982698	34.0	31.0	34.0	28.0	34.0
3	31.435532917908855	34.0	31.0	34.0	28.0	34.0
4	34.66626221288111	37.0	35.0	37.0	32.0	37.0
5	34.77510183286313	37.0	35.0	37.0	32.0	37.0
6	34.79031547197722	37.0	35.0	37.0	32.0	37.0
7	34.73625866288052	37.0	35.0	37.0	32.0	37.0
8	34.71639801966467	37.0	35.0	37.0	32.0	37.0
9	36.22515893512626	39.0	37.0	39.0	32.0	39.0
10-11	36.25330103629594	39.0	37.0	39.0	32.0	39.0
12-13	36.19884230865803	39.0	37.0	39.0	32.0	39.0
14-15	37.60401205380276	41.0	38.0	41.0	32.0	41.0
16-17	37.60875289487039	41.0	38.0	41.0	32.0	41.0
18-19	37.63330321331355	41.0	38.5	41.0	32.0	41.0
20-21	37.685579095573644	41.0	39.0	41.0	32.0	41.0
22-23	37.68236878480734	41.0	38.5	41.0	32.0	41.0
24-25	37.698753015376816	41.0	38.0	41.0	31.5	41.0
26-27	37.70215633396556	41.0	38.0	41.0	31.0	41.0
28-29	37.673981142918436	41.0	38.0	41.0	31.0	41.0
30-31	37.61158657368839	40.5	38.0	41.0	31.0	41.0
32-33	37.54697073531239	40.0	38.0	41.0	31.0	41.0
34-35	37.48530839074344	40.0	38.0	41.0	30.5	41.0
36-37	37.395148846170784	40.0	38.0	41.0	30.0	41.0
38-39	37.30157077154334	40.0	38.0	41.0	30.0	41.0
40-41	37.221577227526595	40.0	38.0	41.0	30.0	41.0
42-43	37.10790609033517	40.0	38.0	41.0	30.0	41.0
44-45	36.980221538211104	40.0	37.5	41.0	30.0	41.0
46-47	36.86374299655213	40.0	37.0	41.0	30.0	41.0
48-49	36.745977703151596	40.0	37.0	41.0	30.0	41.0
50-51	36.62268956104253	40.0	37.0	41.0	30.0	41.0
52-53	36.4695083199805	40.0	36.0	41.0	30.0	41.0
54-55	36.31538209128421	40.0	36.0	41.0	29.0	41.0
56-57	36.13199403947593	40.0	35.5	41.0	29.0	41.0
58-59	35.924781078358606	39.0	35.0	41.0	29.0	41.0
60-61	35.76417950636224	39.0	35.0	41.0	28.5	41.0
62-63	35.61077572352745	39.0	35.0	41.0	28.0	41.0
64-65	35.356986537457445	39.0	35.0	41.0	28.0	41.0
66-67	35.05690718101704	38.0	35.0	40.0	28.0	41.0
68-69	34.74366832603347	37.5	34.0	40.0	27.5	41.0
70-71	34.48407823750867	37.0	34.0	40.0	27.0	41.0
72-73	34.23174579625209	36.5	34.0	39.0	27.0	41.0
74-75	33.8792044640319	36.0	34.0	39.0	26.0	41.0
76-77	33.51486041701938	36.0	34.0	39.0	26.0	40.5
78-79	33.160039340407124	35.0	34.0	37.5	26.0	39.5
80-81	32.80549226773523	35.0	33.5	37.0	26.0	39.0
82-83	32.46286950280829	35.0	33.0	37.0	25.0	39.0
84-85	32.15669874442189	35.0	33.0	36.0	25.0	38.0
86-87	31.875097862077837	35.0	33.0	36.0	25.0	37.0
88-89	31.613543420142072	35.0	33.0	35.5	24.0	37.0
90-91	31.396466415947465	35.0	33.0	35.0	24.0	36.5
92-93	31.182362858262128	35.0	33.0	35.0	23.0	36.0
94-95	30.96607615887171	35.0	32.0	35.0	21.0	36.0
96-97	30.773903287869487	35.0	32.0	35.0	19.5	36.0
98-99	30.649266066518358	35.0	32.0	35.0	18.5	35.5
100	30.60388927554109	35.0	32.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3527728023018497
1101	2	0.3752545798610072
1101	3	0.3416475455682857
1101	4	0.3734930150480338
1101	5	0.26721770307347725
1101	6	0.3014909566950905
1101	7	0.31982674104931164
1101	8	0.2846884588534522
1101	9	0.3476237708559893
1101	10-11	0.35951814019537665
1101	12-13	0.3206135900117033
1101	14-15	0.422441042694814
1101	16-17	0.4099305378179352
1101	18-19	0.37629737925550444
1101	20-21	0.3730964823266021
1101	22-23	0.3779584643087617
1101	24-25	0.38279361410042867
1101	26-27	0.37280600654499096
1101	28-29	0.39802995639304584
1101	30-31	0.42519706566372406
1101	32-33	0.3832038607281234
1101	34-35	0.4240641395017377
1101	36-37	0.4396706515326443
1101	38-39	0.4026344267437594
1101	40-41	0.42508216597686044
1101	42-43	0.43017322864300667
1101	44-45	0.43618270232785505
1101	46-47	0.39183777001211695
1101	48-49	0.40169808850632904
1101	50-51	0.37136222860488743
1101	52-53	0.38814436584360124
1101	54-55	0.3938489381868564
1101	56-57	0.37586922827971847
1101	58-59	0.34803926443677113
1101	60-61	0.41933888087621796
1101	62-63	0.3903131242192188
1101	64-65	0.40862874248583125
1101	66-67	0.3786424059601643
1101	68-69	0.3892083208839736
1101	70-71	0.42777321825278136
1101	72-73	0.4701160962735855
1101	74-75	0.41084887958427885
1101	76-77	0.39475811856129894
1101	78-79	0.3674437345319532
1101	80-81	0.37936680342404117
1101	82-83	0.40194924767799733
1101	84-85	0.38419612419774296
1101	86-87	0.3190864719454751
1101	88-89	0.2962944507424474
1101	90-91	0.333444314814038
1101	92-93	0.34263914446976784
1101	94-95	0.42715694451949204
1101	96-97	0.37004242029682644
1101	98-99	0.3696859691695771
1101	100	0.42265273517112334
1102	1	0.1932194306793633
1102	2	0.24107089861799125
1102	3	0.23244194571891086
1102	4	0.1916431129164664
1102	5	0.20337939159722396
1102	6	0.16326485947286784
1102	7	0.23190925072347568
1102	8	0.24636237483471035
1102	9	0.2633317666052548
1102	10-11	0.2605970002758866
1102	12-13	0.2622115782458465
1102	14-15	0.3373294303546146
1102	16-17	0.3033095314170495
1102	18-19	0.2949123477014268
1102	20-21	0.2540723533015523
1102	22-23	0.1626055959300885
1102	24-25	0.26787777920172573
1102	26-27	0.3108345508778214
1102	28-29	0.2934517692761105
1102	30-31	0.2809959875773984
1102	32-33	0.2630353972575392
1102	34-35	0.2729010520618971
1102	36-37	0.2892537952243046
1102	38-39	0.2738188895737039
1102	40-41	0.2650760710547999
1102	42-43	0.2696307499482842
1102	44-45	0.2377716764742246
1102	46-47	0.20188772999392768
1102	48-49	0.15204006013823346
1102	50-51	0.18901779282794706
1102	52-53	0.17346805131697351
1102	54-55	0.12985353159636048
1102	56-57	0.1588399181929958
1102	58-59	0.17900222432920287
1102	60-61	0.18929665759853975
1102	62-63	0.16881462233045852
1102	64-65	0.16557178601405553
1102	66-67	0.14880293899075525
1102	68-69	0.1818446994890266
1102	70-71	0.16712380293526508
1102	72-73	0.17988497616089205
1102	74-75	0.17918760934301048
1102	76-77	0.20178636608443412
1102	78-79	0.12719815583678695
1102	80-81	0.18392688797402457
1102	82-83	0.18585527003463653
1102	84-85	0.18796435819278656
1102	86-87	0.1804665379739916
1102	88-89	0.2004569829520264
1102	90-91	0.1717222055469314
1102	92-93	0.17653050933648373
1102	94-95	0.09369115208554035
1102	96-97	0.1558222900593904
1102	98-99	0.13173464866479279
1102	100	0.09169862666018602
1103	1	0.04707505358203434
1103	2	0.10270436247256498
1103	3	0.11381283386208452
1103	4	0.06118398807308978
1103	5	0.00784463060297469
1103	6	0.02901189667742443
1103	7	0.08249752096716634
1103	8	0.08425715765091724
1103	9	0.13616222559238622
1103	10-11	0.1328098360034602
1103	12-13	0.13100231858911116
1103	14-15	0.17945680198288017
1103	16-17	0.12821994274684556
1103	18-19	0.09778866782478701
1103	20-21	0.11155653621648298
1103	22-23	0.13104845778617147
1103	24-25	0.12725595956263192
1103	26-27	0.20778519157821762
1103	28-29	0.15661399997153325
1103	30-31	0.12999427656413332
1103	32-33	0.11931594913358623
1103	34-35	0.20904489403722692
1103	36-37	0.19490907080918163
1103	38-39	0.1826093800622104
1103	40-41	0.1545787577804134
1103	42-43	0.15569550403906618
1103	44-45	0.07959145131771095
1103	46-47	-0.0042545951465911
1103	48-49	0.043981063724139347
1103	50-51	0.026343487163494217
1103	52-53	-0.002392595043517076
1103	54-55	-0.012289677282410594
1103	56-57	-0.06463470374429647
1103	58-59	-0.04962105823902618
1103	60-61	-0.010864026554934014
1103	62-63	-0.013823816388139676
1103	64-65	0.028141388762698227
1103	66-67	0.04130017435767286
1103	68-69	-0.018594828478278203
1103	70-71	0.006506224924997639
1103	72-73	-0.06742965570762749
1103	74-75	-0.030876432169868906
1103	76-77	0.0045984125464002545
1103	78-79	0.03415799788136553
1103	80-81	0.019903868284480097
1103	82-83	-0.00929747877103182
1103	84-85	0.021359630586616163
1103	86-87	0.017290565266790736
1103	88-89	-0.012896455492121817
1103	90-91	-0.04379852620093061
1103	92-93	-0.024467306303360914
1103	94-95	-0.06337057954959491
1103	96-97	-0.10894760846220208
1103	98-99	-0.07250626208137945
1103	100	-0.06384068287110267
1104	1	0.2060877266233767
1104	2	0.21680217026840154
1104	3	0.19798584520281182
1104	4	0.16354550244783184
1104	5	0.16560639546510458
1104	6	0.15318373851646072
1104	7	0.24721099878619412
1104	8	0.26221933153289
1104	9	0.25843607320325646
1104	10-11	0.22843693159952494
1104	12-13	0.2571533796061445
1104	14-15	0.2962009035476072
1104	16-17	0.2904676447333472
1104	18-19	0.2931385705000764
1104	20-21	0.32401977430676965
1104	22-23	0.2565107071506816
1104	24-25	0.30388837944889957
1104	26-27	0.3172896381688659
1104	28-29	0.3527762572473847
1104	30-31	0.3766491768717515
1104	32-33	0.39448196295587223
1104	34-35	0.3259063184062043
1104	36-37	0.32363901681718943
1104	38-39	0.33619971202870147
1104	40-41	0.3169043787724277
1104	42-43	0.32641207989099286
1104	44-45	0.32575013511616646
1104	46-47	0.32769923896532305
1104	48-49	0.3062431169486999
1104	50-51	0.22590640501670833
1104	52-53	0.18445443795745575
1104	54-55	0.24447766717290165
1104	56-57	0.29034087621961646
1104	58-59	0.2106533389612011
1104	60-61	0.25425720032112764
1104	62-63	0.24219975747907796
1104	64-65	0.33299995167443797
1104	66-67	0.3359075390079198
1104	68-69	0.320936726855777
1104	70-71	0.28986809511872025
1104	72-73	0.30278520965253364
1104	74-75	0.28243591765330933
1104	76-77	0.2328236765346574
1104	78-79	0.2607731236658566
1104	80-81	0.26530168804287513
1104	82-83	0.28411401307360507
1104	84-85	0.23129268425871174
1104	86-87	0.22085347509400322
1104	88-89	0.24708157239934891
1104	90-91	0.28434979336655175
1104	92-93	0.23733514422308488
1104	94-95	0.206903678151253
1104	96-97	0.23052809934429774
1104	98-99	0.20280116207586119
1104	100	0.22533370626491234
1105	1	0.03074235156328342
1105	2	0.08655574414538592
1105	3	0.10132024606603451
1105	4	0.0779366041347771
1105	5	0.07494746673798858
1105	6	0.09796857140145931
1105	7	0.10703995957901924
1105	8	0.14190879741695284
1105	9	0.1331298153211833
1105	10-11	0.10644843329092879
1105	12-13	0.11358727727328954
1105	14-15	0.1700756846330762
1105	16-17	0.20105265631708136
1105	18-19	0.17781145675772336
1105	20-21	0.1344323003273189
1105	22-23	0.11570145262660958
1105	24-25	0.1369687696012818
1105	26-27	0.11158629077968385
1105	28-29	0.158663983804459
1105	30-31	0.19791957855049702
1105	32-33	0.1703755405029952
1105	34-35	0.16278710065921587
1105	36-37	0.13559986771474541
1105	38-39	0.21344258829611107
1105	40-41	0.1651617598127686
1105	42-43	0.1818690725773351
1105	44-45	0.17675304601493735
1105	46-47	0.15864001939029748
1105	48-49	0.15380827848667877
1105	50-51	0.17221117033432165
1105	52-53	0.14738012070500162
1105	54-55	0.1755378461198518
1105	56-57	0.1733044690272436
1105	58-59	0.1588003055904963
1105	60-61	0.17333048927924466
1105	62-63	0.1932068384571437
1105	64-65	0.2283037496585436
1105	66-67	0.12483897580142411
1105	68-69	0.16546177960717756
1105	70-71	0.2101683962464449
1105	72-73	0.17183717793385966
1105	74-75	0.14547125377731618
1105	76-77	0.15826184903067997
1105	78-79	0.12818159871878976
1105	80-81	0.0911982238680551
1105	82-83	0.13729928823288162
1105	84-85	0.16645385421319503
1105	86-87	0.08607309757869785
1105	88-89	0.11853480971170782
1105	90-91	0.1244060217243863
1105	92-93	0.053874896338118816
1105	94-95	0.07464202267231101
1105	96-97	0.057138836863313
1105	98-99	0.09952154789776202
1105	100	0.11689107730643045
1106	1	0.10421745766137747
1106	2	0.11894680787979439
1106	3	0.15157430572952535
1106	4	0.06733646446227226
1106	5	0.03638835279596009
1106	6	0.08692436714898122
1106	7	0.045549812953062485
1106	8	0.07615143177887518
1106	9	0.08975613454471443
1106	10-11	0.07224987601411925
1106	12-13	0.10188449284503776
1106	14-15	0.11479383058976111
1106	16-17	0.08853043077720457
1106	18-19	0.10625231644746691
1106	20-21	0.13630400318277225
1106	22-23	0.1355383674117121
1106	24-25	0.1796732805875152
1106	26-27	0.2318305349470151
1106	28-29	0.24101142955172605
1106	30-31	0.2190178167017791
1106	32-33	0.14927796477763877
1106	34-35	0.21131203544752708
1106	36-37	0.2649355857078106
1106	38-39	0.21546472623935387
1106	40-41	0.18407931021543078
1106	42-43	0.19756627614076194
1106	44-45	0.17389263566305857
1106	46-47	0.15433894305304818
1106	48-49	0.12621849626675186
1106	50-51	0.11104287070494223
1106	52-53	0.12598062596301673
1106	54-55	0.11809602353881843
1106	56-57	0.11553141508234432
1106	58-59	0.1411447041785081
1106	60-61	0.13637340643816032
1106	62-63	0.22577428229478613
1106	64-65	0.2226933424761839
1106	66-67	0.27907062898155743
1106	68-69	0.2213887628998279
1106	70-71	0.23584704286759006
1106	72-73	0.1382731602919165
1106	74-75	0.16499751840407129
1106	76-77	0.15227633396719398
1106	78-79	0.143956112908036
1106	80-81	0.17512520583584035
1106	82-83	0.16718134016858244
1106	84-85	0.14619260042013593
1106	86-87	0.16741218386039236
1106	88-89	0.16572178213431243
1106	90-91	0.17526346860348596
1106	92-93	0.1848992760227013
1106	94-95	0.18748513837131142
1106	96-97	0.12507830710547552
1106	98-99	0.14899645666653427
1106	100	0.19117896161536407
1107	1	0.17345753164565636
1107	2	0.18944725428427134
1107	3	0.19090742261337468
1107	4	0.1527127799137915
1107	5	0.17324953024008494
1107	6	0.1712847230554999
1107	7	0.15623370645000279
1107	8	0.16943105963157734
1107	9	0.16736650797557218
1107	10-11	0.21023697006194197
1107	12-13	0.17822440371717363
1107	14-15	0.23586957334341463
1107	16-17	0.25179172084993695
1107	18-19	0.22283999186132064
1107	20-21	0.24109138064773106
1107	22-23	0.2751390872482773
1107	24-25	0.24543540943893305
1107	26-27	0.28287918933221334
1107	28-29	0.31001828094461104
1107	30-31	0.2972955349814015
1107	32-33	0.30386932180307014
1107	34-35	0.305007370439057
1107	36-37	0.31595730295349256
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2216	56-57	-0.6319723418514229
2216	58-59	-0.5901426542117463
2216	60-61	-0.6305202053169126
2216	62-63	-0.6585765956099436
2216	64-65	-0.7190314685324353
2216	66-67	-0.7312041987583768
2216	68-69	-0.7626942822333476
2216	70-71	-0.8026615738746017
2216	72-73	-0.7386197379943411
2216	74-75	-0.7833094492684438
2216	76-77	-0.7129254041808792
2216	78-79	-0.6519608450449681
2216	80-81	-0.5785043037507194
2216	82-83	-0.6051991778625165
2216	84-85	-0.574173950568067
2216	86-87	-0.5770822244434761
2216	88-89	-0.620487195267355
2216	90-91	-0.6051129222503882
2216	92-93	-0.7223126803006643
2216	94-95	-0.7067198367031509
2216	96-97	-0.8018528976947792
2216	98-99	-0.9102447629325177
2216	100	-0.8838556393778312
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89147.0
3	17831.0
4	12453.0
5	11179.0
6	7988.0
7	7365.0
8	8043.0
9	8602.0
10	8808.0
11	9473.0
12	11097.0
13	12303.0
14	13353.0
15	14107.0
16	14112.0
17	13675.0
18	13105.0
19	12170.0
20	11879.0
21	12321.0
22	14986.0
23	23211.0
24	37947.0
25	48927.0
26	46652.0
27	36299.0
28	39146.0
29	47691.0
30	57354.0
31	68338.0
32	88092.0
33	118328.0
34	168512.0
35	264889.0
36	460486.0
37	921570.0
38	1534396.0
39	773662.0
40	2474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11183645812035	21.364771841653752	13.731381909522922	22.79200979070298
2	14.830446279261118	22.24505700849374	40.916936407106725	22.00756030513842
3	17.167822013958993	28.762300053141367	30.663397847083253	23.40648008581639
4	11.876746727112208	18.89237918777424	39.92582330149525	29.305050783618302
5	11.899324531486569	40.00284079235586	35.62266691531831	12.475167760839263
6	27.63881895016783	40.79191682449385	18.95010856443073	12.61915566090758
7	24.607292297802573	34.95150406827696	23.95438456680214	16.486819067118322
8	23.408589262957058	37.8763331516518	21.539198861471153	17.17587872391999
9	24.71351574317593	18.575037273030603	21.832799911338885	34.878647072454584
10-11	22.246304848447373	29.04001227980168	29.907638743880593	18.80604412787035
12-13	23.000250297759507	27.24550377708604	29.27636290290877	20.47788302224568
14-15	20.840241874163247	28.761118939638337	26.72270346866863	23.675935717529793
16-17	20.325412769057742	31.686787616918387	27.241088500902116	20.746711113121748
18-19	20.359075545869384	30.662166970138706	29.017896388580656	19.960861095411254
20-21	21.884864611037873	29.76848346227191	28.306770228434736	20.03988169825548
22-23	21.437716652268453	29.822563187343427	28.273611207966226	20.466108952421894
24-25	21.12371840929156	29.90972291228061	28.274223617638267	20.692335060789564
26-27	20.926482984592365	29.81997526260028	28.399145313159636	20.85439643964772
28-29	20.960333435335762	29.73268712918347	28.548494252535228	20.758485182945535
30-31	21.1657771251554	30.02079624715353	28.25939737702962	20.55402925066145
32-33	20.755245338228924	29.89253593116199	28.576151463530707	20.77606726707838
34-35	21.077530866929106	29.612101689243183	28.485050586026667	20.82531685780104
36-37	21.31607431176512	29.7430980936082	28.14660534404484	20.79422225058184
38-39	21.072631589552763	29.800862154287334	28.34137137490515	20.785134881254752
40-41	20.943808251765965	29.60539481557678	28.59084929565973	20.859947636997525
42-43	20.917316594662434	29.66339593806444	28.520027080360595	20.89926038691253
44-45	20.99442094788769	29.700347947469474	28.460228239158226	20.84500286548461
46-47	21.018225904494514	29.735709667242265	28.40678067890946	20.83928374935376
48-49	20.95969139293765	29.541338739396178	28.491293213651364	21.00767665401481
50-51	20.9370915005242	29.52464563704533	28.419364709912404	21.118898152518064
52-53	20.954456277999224	29.554969793386803	28.42255516675224	21.068018761861733
54-55	21.04151722718285	29.8456470809493	28.300981969276396	20.811853722591454
56-57	20.985817579753025	29.648846269565748	28.282530658512268	21.08280549216896
58-59	20.94825316067595	29.337445828907356	28.48081310619915	21.233487904217547
60-61	21.18364565897355	29.637921671222532	28.190728473157982	20.987704196645932
62-63	20.92288754716295	29.603231626573915	28.38730210030836	21.086578725954773
64-65	20.980604292340306	29.466232566942065	28.486446137921984	21.066717002795652
66-67	20.90848604229459	29.505601671759873	28.317052784379836	21.268859501565696
68-69	21.198926268048552	29.63443488712203	28.24876910594707	20.917869738882345
70-71	21.18494949892048	29.469015132642994	28.322762022935333	21.023273345501188
72-73	21.038287260041592	29.491308820220425	28.42966702100822	21.040736898729765
74-75	21.012328992007266	29.574724944097863	28.38242257808273	21.030523485812147
76-77	21.09669336311883	29.573668043534823	28.159021456266736	21.17061713707961
78-79	21.141626453411135	29.608180291827036	28.27262345043067	20.977569804331157
80-81	21.045942380942126	29.549645780270172	28.35336275138676	21.051049087400937
82-83	20.991707667703384	29.533933014754844	28.493447851432414	20.980911466109358
84-85	21.044525153565242	29.396022648644447	28.337040577257337	21.22241162053297
86-87	21.181084870114624	29.504887043031523	28.19703963260476	21.116988454249096
88-89	21.011466176906815	29.462510424897225	28.309620262592333	21.216403135603628
90-91	21.001687829482105	29.424151538975114	28.3163794729037	21.25778115863908
92-93	21.032388871921043	29.449538529497644	28.320606132683977	21.19746646589733
94-95	21.028790263672448	29.376243056539902	28.35369554813515	21.241271131652496
96-97	21.05356060888924	29.482732972557784	28.206624306852422	21.25708211170055
98-99	20.878509973688438	29.453439245998027	28.336040021782576	21.33201075853096
100	20.962301146325682	29.42778543311948	28.23341383789443	21.376499582660404
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15690.0
1	11178.0
2	5550.0
3	3674.0
4	2479.0
5	1858.5
6	1610.0
7	1706.0
8	1931.0
9	1932.0
10	1630.0
11	1324.0
12	1329.0
13	1441.5
14	1515.0
15	1707.5
16	2035.5
17	2544.0
18	3275.5
19	4347.5
20	6175.5
21	8692.5
22	12437.5
23	18647.0
24	25963.0
25	34699.0
26	46642.5
27	60947.0
28	77795.0
29	96037.5
30	111976.5
31	126260.0
32	139195.0
33	149345.0
34	157953.0
35	167021.0
36	174805.5
37	180842.5
38	184837.0
39	186330.5
40	188362.0
41	188169.0
42	184315.0
43	180203.0
44	174972.5
45	170273.0
46	166174.0
47	158477.0
48	149739.5
49	143767.5
50	139715.0
51	132825.0
52	122286.0
53	112997.0
54	106733.0
55	101457.0
56	92396.5
57	81154.5
58	73504.0
59	67326.0
60	58722.0
61	49109.0
62	40878.5
63	33292.0
64	26757.0
65	21304.0
66	16585.5
67	12730.5
68	9724.0
69	7427.0
70	5544.5
71	4226.5
72	3255.0
73	2345.5
74	1738.5
75	1328.0
76	961.5
77	704.0
78	526.0
79	401.0
80	322.0
81	235.5
82	182.5
83	161.5
84	145.0
85	122.0
86	101.0
87	100.5
88	90.5
89	74.0
90	58.0
91	45.5
92	42.0
93	38.0
94	29.5
95	27.0
96	29.0
97	30.0
98	25.5
99	35.0
100	90.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.136339184874825E-4
2	1.1853090426634211E-4
3	1.9755150711057017E-5
4	7.902060284422807E-5
5	5.9265452133171054E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	9.877575355528508E-6
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.0865332891081359E-4
84-85	6.815526995314671E-4
86-87	0.0012939623715742346
88-89	0.0033682531962352215
90-91	0.002854619277747739
92-93	0.0021533114275052146
94-95	0.0020446580985944013
96-97	0.0019360047696835875
98-99	0.0025187817156597696
100	0.002884252003814324
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5061971.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.552761391093924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.95027332057033	46.749490512894326
2	14.525075097471715	17.881169633247936
3	4.562930083967283	8.425828401080468
4	1.8875332553802793	4.647315363447084
5	0.9820840706794117	3.0224993234262025
6	0.5371787457271622	1.983890109606668
7	0.3344214641419798	1.4409195210474088
8	0.2375260940388233	1.1696309592424186
9	0.15558688868845857	0.8619122371521043
>10	0.7506802015361581	8.167999058089043
>50	0.05047128552941034	2.1306282089111046
>100	0.025434740387740504	2.791767247031987
>500	6.438022125825207E-4	0.2541526936966073
>1k	1.2875973497008798E-4	0.09684982071335398
>5k	0.0	0.0
>10k+	3.2189933740211236E-5	0.375946910413422
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18974	0.37483422959159585	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.9755150711057017E-5	0.0
3	0.0	0.0	0.0	5.9265452133171054E-5	0.0
4	0.0	0.0	0.0	5.9265452133171054E-5	0.0
5	0.0	0.0	0.0	7.902060284422807E-5	0.0
6	0.0	0.0	0.0	7.902060284422807E-5	0.0
7	0.0	0.0	0.0	7.902060284422807E-5	0.0
8	0.0	0.0	0.0	1.1853090426634211E-4	0.0
9	0.0	0.0	0.0	1.5804120568845614E-4	0.0
10-11	0.0	0.0	0.0	1.7779635639951315E-4	0.0
12-13	0.0	0.0	0.0	1.8767393175504166E-4	0.0
14-15	0.0	0.0	0.0	2.0742908246609867E-4	0.0
16-17	0.0	0.0	0.0	2.5681695924374123E-4	0.0
18-19	0.0	0.0	0.0	2.7657210995479824E-4	0.0
20-21	0.0	0.0	0.0	3.259599867324408E-4	0.0
22-23	0.0	0.0	0.0	4.4449089099878286E-4	0.0
24-25	0.0	0.0	0.0	6.914302748869956E-4	0.0
26-27	0.0	0.0	0.0	0.001106288439819193	0.0
28-29	0.0	0.0	0.0	0.002311352633193671	0.0
30-31	0.0	0.0	0.0	0.006242627624694018	0.0
32-33	0.0	0.0	0.0	0.015260853924291547	0.0
34-35	0.0	0.0	0.0	0.028990683668476173	0.0
36-37	0.0	0.0	0.0	0.0485087725710005	0.0
38-39	0.0	0.0	0.0	0.0750794502773722	0.0
40-41	0.0	0.0	0.0	0.10101796316099006	0.0
42-43	0.0	0.0	0.0	0.1280627644844271	0.0
44-45	0.0	0.0	0.0	0.16124154010364738	0.0
46-47	0.0	0.0	0.0	0.20320148021393247	0.0
48-49	0.0	0.0	0.0	0.247561671135611	0.0
50-51	0.0	0.0	0.0	0.2924947614279102	0.0
52-53	0.0	0.0	0.0	0.3351461318130823	0.0
54-55	9.877575355528508E-6	0.0	0.0	0.38401049709688184	0.0
56-57	2.9632726066585527E-5	0.0	0.0	0.44352288861394107	0.0
58-59	3.9510301422114034E-5	0.0	0.0	0.5123399561159082	0.0
60-61	3.9510301422114034E-5	0.0	0.0	0.586925527625504	0.0
62-63	5.9265452133171054E-5	0.0	0.0	0.6620444882042983	0.0
64-65	5.9265452133171054E-5	0.0	0.0	0.7420627261594347	0.0
66-67	5.9265452133171054E-5	0.0	0.0	0.83710475623033	0.0
68-69	5.9265452133171054E-5	0.0	0.0	0.9475656814312053	0.0
70-71	5.9265452133171054E-5	0.0	0.0	1.0692672873866722	0.0
72-73	5.9265452133171054E-5	0.0	0.0	1.1994537305725377	0.0
74-75	5.9265452133171054E-5	0.0	0.0	1.334094960243747	0.0
76-77	5.9265452133171054E-5	0.0	0.0	1.4804411167112574	0.0
78-79	7.902060284422807E-5	1.9755150711057017E-5	0.0	1.6513034152111894	0.0
80-81	7.902060284422807E-5	1.9755150711057017E-5	0.0	1.8445186667406825	0.0
82-83	7.902060284422807E-5	1.9755150711057017E-5	0.0	2.049251171134722	0.0
84-85	7.902060284422807E-5	1.9755150711057017E-5	0.0	2.256907437833998	0.0
86-87	7.902060284422807E-5	1.9755150711057017E-5	0.0	2.478471330633858	0.0
88	7.902060284422807E-5	1.9755150711057017E-5	0.0	2.663093091604041	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5500	0.0	40.932423	1
GTATCAA	9450	0.0	36.60502	1
TCAACGC	10035	0.0	32.6445	4
ATCAACG	10130	0.0	32.57034	3
CAACGCA	10800	0.0	30.375698	5
TATCAAC	11870	0.0	28.310658	2
AACGCAG	11755	0.0	27.907915	6
GTACATG	21270	0.0	26.891666	1
TACATGG	20705	0.0	26.581305	2
ACATGGG	21110	0.0	25.024921	3
CGCAGAG	13080	0.0	24.470001	8
ACGCAGA	13325	0.0	24.302258	7
TACCTGG	3750	0.0	23.687824	2
GTACCTG	4310	0.0	21.155294	1
CATGGGG	12030	0.0	20.862686	4
GCAGAGT	15300	0.0	20.182203	9
CATGGGT	4845	0.0	19.498308	4
CATGGGA	6995	0.0	18.678917	4
ACCTGGG	4865	0.0	17.67921	3
AGAGTAC	13525	0.0	17.357727	10-11
>>END_MODULE