FastQCFastQC Report
Wed 25 May 2016
SRR2431353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431353_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5061971
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247570.48907826615363853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179800.3551976097848052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142680.2818664903453615No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50660.10007959350221485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96050.059.306811
GTATCAA195250.046.338181
ATCAACG250700.035.6939663
TCAACGC253500.035.299714
TATCAAC261750.034.4025762
CAACGCA262850.034.0798035
AACGCAG274850.032.6260726
ACGCAGA312550.028.6305457
CGCAGAG316000.028.2138548
GTACATG202550.026.476021
TACATGG197000.026.290422
GTGGTAT35350.025.9265371
GCAGAGT343650.025.7386289
ACATGGG202200.024.75433
TGGTATC36100.023.6944352
TACCTGG39000.022.7760982
AGAGTAC323150.022.04840710-11
GAGTACT231250.021.45154812-13
CATGGGG108000.021.2797874
AGTACTT241150.020.89246412-13
Produced by FastQC (version 0.11.5)