##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431353_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5061971
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.756227169219265	34.0	31.0	34.0	31.0	34.0
2	32.88430810844235	34.0	31.0	34.0	31.0	34.0
3	32.958231289748596	34.0	31.0	34.0	31.0	34.0
4	36.34423824237634	37.0	37.0	37.0	35.0	37.0
5	36.294985885932576	37.0	37.0	37.0	35.0	37.0
6	36.32467649459074	37.0	37.0	37.0	35.0	37.0
7	36.193096127970705	37.0	37.0	37.0	35.0	37.0
8	36.24977760639087	37.0	37.0	37.0	35.0	37.0
9	38.07061715683476	39.0	39.0	39.0	35.0	39.0
10-11	38.06259379992497	39.0	39.0	39.0	35.0	39.0
12-13	38.0426147245806	39.0	39.0	39.0	35.0	39.0
14-15	39.542161837750555	41.0	40.0	41.0	37.0	41.0
16-17	39.55413622480255	41.0	39.5	41.0	37.0	41.0
18-19	39.621908541159165	41.0	40.0	41.0	37.0	41.0
20-21	39.62652936968624	41.0	40.0	41.0	37.0	41.0
22-23	39.5548320012106	41.0	40.0	41.0	36.5	41.0
24-25	39.52968329925241	41.0	40.0	41.0	36.0	41.0
26-27	39.447776370113544	41.0	39.5	41.0	36.0	41.0
28-29	39.36252538783806	41.0	39.0	41.0	36.0	41.0
30-31	39.21352799136937	41.0	39.0	41.0	35.5	41.0
32-33	39.029077606331604	41.0	39.0	41.0	35.0	41.0
34-35	38.92777842860024	41.0	39.0	41.0	35.0	41.0
36-37	38.82382514637085	41.0	39.0	41.0	35.0	41.0
38-39	38.71139917632875	41.0	38.0	41.0	35.0	41.0
40-41	38.62392494939224	41.0	38.0	41.0	35.0	41.0
42-43	38.50665215189893	41.0	38.0	41.0	35.0	41.0
44-45	38.32606310861915	41.0	38.0	41.0	34.5	41.0
46-47	38.053389282554164	41.0	38.0	41.0	34.0	41.0
48-49	37.820729415478674	40.0	38.0	41.0	33.0	41.0
50-51	37.690082776057	40.0	38.0	41.0	33.0	41.0
52-53	37.588808687367035	40.0	38.0	41.0	33.0	41.0
54-55	37.453494399711104	40.0	37.0	41.0	33.0	41.0
56-57	37.30495097660575	40.0	37.0	41.0	33.0	41.0
58-59	37.1341019140568	40.0	37.0	41.0	32.5	41.0
60-61	36.949239732902456	40.0	36.0	41.0	32.0	41.0
62-63	36.735493249566225	39.5	36.0	41.0	32.0	41.0
64-65	36.473717056063734	39.0	35.0	41.0	31.5	41.0
66-67	36.19475674593948	39.0	35.0	41.0	31.0	41.0
68-69	35.93014815770378	38.5	35.0	41.0	31.0	41.0
70-71	35.67397887107611	37.5	35.0	40.0	31.0	41.0
72-73	35.4044800928334	37.0	35.0	40.0	31.0	41.0
74-75	35.09211747755963	36.5	35.0	39.0	31.0	41.0
76-77	34.75466315393747	36.0	35.0	39.0	31.0	41.0
78-79	34.415125748448574	36.0	35.0	38.5	30.5	40.0
80-81	34.084802639130096	35.0	35.0	37.0	30.0	39.0
82-83	33.74513899822816	35.0	35.0	37.0	30.0	39.0
84-85	33.460688139066775	35.0	34.5	36.5	30.0	38.5
86-87	33.21851399385733	35.0	34.0	36.0	30.0	37.0
88-89	32.980702576130916	35.0	34.0	36.0	29.0	37.0
90-91	32.77276163770989	35.0	34.0	35.5	29.0	36.5
92-93	32.6158751205805	35.0	34.0	35.0	29.0	36.0
94-95	32.478053114093306	35.0	34.0	35.0	29.0	36.0
96-97	32.337683285818905	35.0	34.0	35.0	29.0	36.0
98-99	32.21618940527316	35.0	34.0	35.0	29.0	36.0
100	32.114058338145355	35.0	34.0	35.0	29.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18335282683416665
1101	2	0.19082353106276173
1101	3	0.18647140280673824
1101	4	0.0996001231897452
1101	5	0.13339567739912894
1101	6	0.1487026491253829
1101	7	0.17929370333859396
1101	8	0.15841000463565535
1101	9	0.2445442610382429
1101	10-11	0.22092610602584273
1101	12-13	0.19986738184771013
1101	14-15	0.28747883139794794
1101	16-17	0.3013542837647165
1101	18-19	0.25754851264770906
1101	20-21	0.26339418713863694
1101	22-23	0.32797061144269435
1101	24-25	0.2906367650084718
1101	26-27	0.27049213117999216
1101	28-29	0.27011786150480077
1101	30-31	0.332964509540318
1101	32-33	0.3427231712210741
1101	34-35	0.3697108253574797
1101	36-37	0.34426926132791635
1101	38-39	0.3326560873532358
1101	40-41	0.346344401927432
1101	42-43	0.3542533262803005
1101	44-45	0.34101514693206525
1101	46-47	0.3720667731564049
1101	48-49	0.344915081178776
1101	50-51	0.33565660750802095
1101	52-53	0.3207576226207536
1101	54-55	0.3544791924750399
1101	56-57	0.34017968556942435
1101	58-59	0.3771670365789319
1101	60-61	0.3588661425776749
1101	62-63	0.3527197982791819
1101	64-65	0.3908751370785808
1101	66-67	0.3615825734239806
1101	68-69	0.33915070778176215
1101	70-71	0.3302504835454272
1101	72-73	0.28274190607758953
1101	74-75	0.28269242222318525
1101	76-77	0.26004658104896805
1101	78-79	0.2315490891829839
1101	80-81	0.2785262280267986
1101	82-83	0.2903246552447101
1101	84-85	0.2734364267973106
1101	86-87	0.24144458117496015
1101	88-89	0.2184497719452949
1101	90-91	0.24027969369032576
1101	92-93	0.2785979400862928
1101	94-95	0.27051858981130295
1101	96-97	0.2529162877176816
1101	98-99	0.29554277613362956
1101	100	0.3627978117818813
1102	1	0.08712484404888698
1102	2	0.14526873263736206
1102	3	0.13565957995482592
1102	4	0.09507913588795702
1102	5	0.10746035390659614
1102	6	0.09012726962687623
1102	7	0.11099781625533467
1102	8	0.13919729543624015
1102	9	0.18281759082333338
1102	10-11	0.17672691159305742
1102	12-13	0.17202590837951703
1102	14-15	0.2515471753609546
1102	16-17	0.28496581170848856
1102	18-19	0.26184489365795116
1102	20-21	0.2450912607207485
1102	22-23	0.22601092154894786
1102	24-25	0.2304111697490896
1102	26-27	0.23936006216339933
1102	28-29	0.19324408073287458
1102	30-31	0.204298988368393
1102	32-33	0.171923373517906
1102	34-35	0.14087306624720952
1102	36-37	0.08584133391052262
1102	38-39	0.10300328507945267
1102	40-41	0.16842080355164768
1102	42-43	0.17970587354677292
1102	44-45	0.1756889739221279
1102	46-47	0.20492959754908924
1102	48-49	0.23175238670049936
1102	50-51	0.14724331649636468
1102	52-53	0.1565388469430431
1102	54-55	0.12377355318232475
1102	56-57	0.12896774208412864
1102	58-59	0.15840960862887954
1102	60-61	0.13110822217255702
1102	62-63	0.07618500751650004
1102	64-65	0.1195016465325125
1102	66-67	0.11768286598104538
1102	68-69	0.10909815126210987
1102	70-71	0.10922183088760562
1102	72-73	0.10071203248605798
1102	74-75	0.07084773425148683
1102	76-77	0.058534896370105116
1102	78-79	0.07827869666419929
1102	80-81	0.05811046889194671
1102	82-83	0.0856664237921052
1102	84-85	0.08680635101237755
1102	86-87	0.07848458134363057
1102	88-89	0.09864613457251181
1102	90-91	0.12263545525741648
1102	92-93	0.079717154510071
1102	94-95	0.09541354977103111
1102	96-97	0.08737620963696457
1102	98-99	0.11064007230909567
1102	100	0.18331254129618912
1103	1	0.13605256382123088
1103	2	0.14928877767810889
1103	3	0.1445671578922969
1103	4	0.13079390641603794
1103	5	0.10903910153617602
1103	6	0.1574246851253065
1103	7	0.07003759096896545
1103	8	0.12220858828116832
1103	9	0.18882716832680302
1103	10-11	0.20341414438863836
1103	12-13	0.16417507836003864
1103	14-15	0.22138384314049375
1103	16-17	0.2613699708655801
1103	18-19	0.22898375958883577
1103	20-21	0.2343703274364941
1103	22-23	0.24227873870169958
1103	24-25	0.20176878411223242
1103	26-27	0.21475925432591225
1103	28-29	0.25669336897142614
1103	30-31	0.26589791521269035
1103	32-33	0.2684071301058566
1103	34-35	0.2635549305099758
1103	36-37	0.1882813230270841
1103	38-39	0.18282209316208053
1103	40-41	0.18808648804647277
1103	42-43	0.1942218055256859
1103	44-45	0.21099513355020605
1103	46-47	0.11873657156596806
1103	48-49	0.03957263410392642
1103	50-51	0.0728004876919286
1103	52-53	0.10483312587893323
1103	54-55	0.06346564752197281
1103	56-57	0.03163534495198661
1103	58-59	0.027823657902992238
1103	60-61	-0.007731680537531815
1103	62-63	0.01417344652035979
1103	64-65	0.039562721266037215
1103	66-67	0.07324823319966356
1103	68-69	0.0455103903680012
1103	70-71	-0.024751619191512475
1103	72-73	-0.03275150731059284
1103	74-75	-0.03137014972264751
1103	76-77	-0.042507568512021976
1103	78-79	-0.06603295642408114
1103	80-81	0.009162318082751142
1103	82-83	0.025143780753701606
1103	84-85	0.0042881278456476934
1103	86-87	-0.012253735536333465
1103	88-89	-0.00950484577297317
1103	90-91	0.06413852443787249
1103	92-93	0.039652815925791174
1103	94-95	0.015691122670091318
1103	96-97	0.04308836160846852
1103	98-99	0.05964494211607274
1103	100	0.10290489243538303
1104	1	0.09218816715988964
1104	2	0.1404012910022061
1104	3	0.13706184258356302
1104	4	0.05321600556455053
1104	5	0.07371285069488209
1104	6	0.07317640144547255
1104	7	0.08007971213928045
1104	8	0.12733305039638054
1104	9	0.14696141565501364
1104	10-11	0.14607804374422528
1104	12-13	0.16703446221610108
1104	14-15	0.2136621318002625
1104	16-17	0.26828895879562964
1104	18-19	0.23347599329502344
1104	20-21	0.18733935207590946
1104	22-23	0.2075411370439113
1104	24-25	0.20664058991074796
1104	26-27	0.18795468646634106
1104	28-29	0.14981190255389265
1104	30-31	0.16898913558006257
1104	32-33	0.16183745436740082
1104	34-35	0.169475465927718
1104	36-37	0.21172698457711903
1104	38-39	0.21973453799771647
1104	40-41	0.2096853879505005
1104	42-43	0.2263242829200962
1104	44-45	0.18454905044449532
1104	46-47	0.20062062732139196
1104	48-49	0.21019440770292164
1104	50-51	0.1637919040845972
1104	52-53	0.1502213526659446
1104	54-55	0.14976412308034526
1104	56-57	0.12372032186196691
1104	58-59	0.12835732904141395
1104	60-61	0.15440180393923697
1104	62-63	0.17521098179172157
1104	64-65	0.22211893474061384
1104	66-67	0.2566583740023205
1104	68-69	0.2439387291519992
1104	70-71	0.28008534997988477
1104	72-73	0.26441436204383706
1104	74-75	0.22585590883880968
1104	76-77	0.20116290489538358
1104	78-79	0.23745084005358308
1104	80-81	0.2609268706394374
1104	82-83	0.28969648075472776
1104	84-85	0.27798749673515033
1104	86-87	0.23295328537213322
1104	88-89	0.2590021076025124
1104	90-91	0.267923505819482
1104	92-93	0.2854920843528035
1104	94-95	0.2860571442126201
1104	96-97	0.306583933362937
1104	98-99	0.2626496250912993
1104	100	0.22871934908145874
1105	1	-0.0371167030634183
1105	2	0.044199532692275056
1105	3	0.08587592433053715
1105	4	-0.05239096947749289
1105	5	-0.0494630176865698
1105	6	-0.07799217302433448
1105	7	-0.40329948751303135
1105	8	-0.3700579864269997
1105	9	-0.31219085269249547
1105	10-11	-0.31526777997503075
1105	12-13	-0.18272972828857803
1105	14-15	-0.15206593984657957
1105	16-17	-0.11458193414460283
1105	18-19	-0.0794591770171067
1105	20-21	-0.04972483561098784
1105	22-23	-0.04489470051993294
1105	24-25	-0.05604785688946379
1105	26-27	-0.12512833920737876
1105	28-29	-0.15367134496725754
1105	30-31	-0.15514329279078254
1105	32-33	-0.1587899901635197
1105	34-35	-0.2258266929049597
1105	36-37	-0.1522157389447898
1105	38-39	-0.1834799999172958
1105	40-41	-0.17303808868655324
1105	42-43	-0.19278008161719384
1105	44-45	-0.20541773878646552
1105	46-47	-0.22210893525075193
1105	48-49	-0.27167513348376104
1105	50-51	-0.2736347585242669
1105	52-53	-0.25357160080940133
1105	54-55	-0.22188242677518843
1105	56-57	-0.2601115528737665
1105	58-59	-0.2481227564005266
1105	60-61	-0.25979295082302656
1105	62-63	-0.19564349692026184
1105	64-65	-0.15333812507288513
1105	66-67	-0.14254479936362685
1105	68-69	-0.19911623307843485
1105	70-71	-0.21529229177608045
1105	72-73	-0.19566453421715835
1105	74-75	-0.22918373833149985
1105	76-77	-0.20071231946347012
1105	78-79	-0.19306574554403966
1105	80-81	-0.25054051567195046
1105	82-83	-0.26685879997844353
1105	84-85	-0.23890307086162466
1105	86-87	-0.2524864934922846
1105	88-89	-0.24802578341763848
1105	90-91	-0.2256525816774868
1105	92-93	-0.20988906011366026
1105	94-95	-0.24103405786064513
1105	96-97	-0.2726048381063393
1105	98-99	-0.3042375295580335
1105	100	-0.2963355098043863
1106	1	0.08682302213912152
1106	2	0.05605864963914087
1106	3	0.06231350773525435
1106	4	0.04125285319189942
1106	5	0.06304971308168916
1106	6	0.06653922770343712
1106	7	0.10668466045054714
1106	8	0.08990290210623897
1106	9	0.11280174883793848
1106	10-11	0.1066588475580943
1106	12-13	0.10328180134507647
1106	14-15	0.14011789976141387
1106	16-17	0.1695218088213366
1106	18-19	0.1323944049931285
1106	20-21	0.12008763657746613
1106	22-23	0.14532427154628635
1106	24-25	0.1516542742396254
1106	26-27	0.12436934413275935
1106	28-29	0.1306857483143986
1106	30-31	0.13210226274067338
1106	32-33	0.09479937851862985
1106	34-35	0.07638781168720499
1106	36-37	0.09701233704333845
1106	38-39	0.09337992233707837
1106	40-41	0.09717392017913085
1106	42-43	0.0678399814350854
1106	44-45	0.10783747392135012
1106	46-47	0.12216002888566635
1106	48-49	0.08449501164584916
1106	50-51	0.10984443823792844
1106	52-53	0.1413853763747639
1106	54-55	0.09646741172812767
1106	56-57	0.14195359491282034
1106	58-59	0.10789020057698906
1106	60-61	0.0970632283568591
1106	62-63	0.052150693747890386
1106	64-65	0.07294053591186156
1106	66-67	0.11281113965925726
1106	68-69	0.12304897635240053
1106	70-71	0.07830352709451915
1106	72-73	0.09754796085076123
1106	74-75	0.10385567109811689
1106	76-77	0.10957667875145916
1106	78-79	0.1045116211579682
1106	80-81	0.10951632157657087
1106	82-83	0.11865301393713423
1106	84-85	0.11428806207420905
1106	86-87	0.11480074114241745
1106	88-89	0.15391965848383649
1106	90-91	0.1579160149836838
1106	92-93	0.1225448184164577
1106	94-95	0.13749924612721998
1106	96-97	0.10668391664866306
1106	98-99	0.09464513087108628
1106	100	0.13652750296134997
1107	1	0.028664071275770198
1107	2	0.059700245488876647
1107	3	0.0655551925621154
1107	4	-0.026085709969422055
1107	5	-0.041156015926546274
1107	6	-0.04666936787292286
1107	7	-0.03344314392037262
1107	8	-0.010456078585832529
1107	9	0.011148570363026522
1107	10-11	0.04557207346699954
1107	12-13	0.09086662069965712
1107	14-15	0.07507871414610179
1107	16-17	0.10665540194330703
1107	18-19	0.1039841545092628
1107	20-21	0.09475462382975763
1107	22-23	0.1131664992833592
1107	24-25	0.09122575648359543
1107	26-27	0.04715159120972601
1107	28-29	0.07431354013216662
1107	30-31	0.08675263188786175
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2216	40-41	-0.515961711309366
2216	42-43	-0.5251176759160003
2216	44-45	-0.5582740029021522
2216	46-47	-0.5602581523310945
2216	48-49	-0.5311523218675518
2216	50-51	-0.4529234229405077
2216	52-53	-0.47905449384517595
2216	54-55	-0.5599901137448597
2216	56-57	-0.5927181040797649
2216	58-59	-0.6289573749041821
2216	60-61	-0.6305480541915784
2216	62-63	-0.6962752700305117
2216	64-65	-0.6318206381867881
2216	66-67	-0.6236374417820869
2216	68-69	-0.6348161686701701
2216	70-71	-0.5770088500632227
2216	72-73	-0.5712478827823517
2216	74-75	-0.516806670651313
2216	76-77	-0.4914620159759906
2216	78-79	-0.47087234248143517
2216	80-81	-0.4808117977670392
2216	82-83	-0.5144884459435204
2216	84-85	-0.43520751855740514
2216	86-87	-0.4046689124868479
2216	88-89	-0.3769855984963826
2216	90-91	-0.39263288606532143
2216	92-93	-0.5628274968555118
2216	94-95	-0.5253888414208738
2216	96-97	-0.6032560015894397
2216	98-99	-0.5511913885929118
2216	100	-0.5551226785916334
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61.0
3	363.0
4	228.0
5	364.0
6	563.0
7	866.0
8	1539.0
9	2212.0
10	3334.0
11	4910.0
12	7704.0
13	11492.0
14	12208.0
15	15684.0
16	19873.0
17	30268.0
18	37393.0
19	19986.0
20	12822.0
21	11430.0
22	11380.0
23	11969.0
24	13576.0
25	16420.0
26	20423.0
27	25238.0
28	31992.0
29	40248.0
30	50093.0
31	62825.0
32	79364.0
33	101915.0
34	140920.0
35	223297.0
36	381098.0
37	866433.0
38	1725269.0
39	1057892.0
40	8319.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.35816500935786	21.58778817189069	14.725204034599098	24.328842784152346
2	13.045513900714544	22.173244803900456	41.200461480411775	23.580779814973223
3	15.054243939289774	28.665900953545947	32.02393623956627	24.25591886759801
4	10.29002124920681	18.59485372501203	40.371853216896	30.743271808885154
5	11.36268856538293	38.91717277716526	36.20593638327837	13.51420227417344
6	27.668175104124458	40.26828679974658	18.781794680372528	13.28174341575643
7	24.196069080601212	34.32854514575449	24.50183930330695	16.973546470337343
8	24.095732670139753	35.91097222801158	21.715434560964493	18.277860540884173
9	25.251329966133746	16.00398342858938	22.537979771120774	36.20670683415611
10-11	22.457813369535305	27.51902371625598	30.44554186501661	19.577621049192103
12-13	23.801637741504248	25.7273006897906	30.25715674783597	20.213904820869182
14-15	21.183230800808616	26.98514076828966	28.52390896747532	23.3077194634264
16-17	19.652898050976585	30.26259929185687	28.83364997547398	21.250852681692567
18-19	19.59011618201685	28.40613863651135	32.24928590068967	19.75445928078213
20-21	20.948371691580217	27.599061709361827	31.910860413858554	19.541706185199402
22-23	20.507567663193278	27.68451306118147	31.87887195201153	19.929047323613723
24-25	20.213363656190637	27.820691478097054	31.825665432890922	20.140279432821384
26-27	20.051134464439382	27.7497911871082	31.934938881237024	20.26413546721539
28-29	20.12105855661195	27.695046385958875	32.04061177845093	20.143283278978245
30-31	20.33428241935444	27.945761339026205	31.77907450531052	19.94088173630884
32-33	19.982379662269945	27.863909907306038	31.97971774467184	20.17399268575217
34-35	20.26826728658947	27.712724495725798	31.77885398508467	20.240154232600062
36-37	20.56010984289108	27.842011942451016	31.345792730810835	20.25208548384707
38-39	20.37940875168256	27.939609820481625	31.410594092139643	20.27038733569617
40-41	20.251430544754324	27.86116112355784	31.54455765745574	20.3428506742321
42-43	20.297041683248718	27.928311019301947	31.320966358909747	20.45368093853959
44-45	20.453866592913798	28.03424569582868	31.061270854117485	20.450616857140037
46-47	20.610903960137453	28.220212043398053	30.587840990763354	20.58104300570114
48-49	20.712147307523775	28.22009006200505	30.282005813689317	20.785756816781863
50-51	20.67179600797997	28.292762622746075	30.092167532757063	20.94327383651689
52-53	20.63282470729531	28.43274175540928	29.99760666325495	20.936826874040452
54-55	20.772057970132664	28.713501107231075	29.87353790331654	20.640903019319722
56-57	20.673620336426914	28.44568323376166	29.92193790058202	20.958758529229407
58-59	20.586641216586028	28.082469640318415	30.197253599932278	21.133635543163283
60-61	20.816419984893184	28.34792931227045	29.92474150542869	20.910909197407683
62-63	20.59374698116603	28.27713750236815	30.119157142543884	21.009958373921936
64-65	20.588294559569782	28.189809858649923	30.242389772679456	20.979505809100843
66-67	20.58452101215354	28.21193339035364	30.058251387635117	21.145294209857703
68-69	20.89510561071558	28.29318962555653	29.86821848307536	20.943486280652532
70-71	20.895314364894595	28.1531933539509	29.858958097357206	21.092534183797294
72-73	20.730656679508183	28.270452968184106	29.92496790279352	21.07392244951419
74-75	20.73079133418901	28.34157699458441	29.820269943769784	21.107361727456794
76-77	20.811333015998663	28.306107513630852	29.65446047657293	21.228098993797552
78-79	20.87613456286764	28.38439749055521	29.671113697746694	21.068354248830456
80-81	20.677863641998385	28.329684364617663	29.78217311273906	21.210278880644893
82-83	20.604986150460075	28.301748690857448	29.89298881981295	21.20027633886953
84-85	20.641949319333087	28.239950113697187	29.71744561663032	21.4006549503394
86-87	20.716483409241025	28.30092773361778	29.62104892384066	21.361539933300534
88-89	20.536134705953764	28.239359552673854	29.72067106429118	21.5038346770812
90-91	20.49980926100542	28.22155791185547	29.709331947290053	21.569300879849052
92-93	20.527632974062954	28.2753500559975	29.687308925442622	21.509708044496925
94-95	20.46745333833012	28.13006416343848	29.726001038736904	21.676481459494497
96-97	20.4672579721586	28.236017638213536	29.61584898645268	21.680875403175182
98-99	20.283965180340086	28.15456118539989	29.678005268031292	21.88346836622873
100	20.442062952542894	28.13365487820273	29.56719262446084	21.857089544793535
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2123.0
1	1398.5
2	2330.5
3	3386.0
4	1838.0
5	1183.5
6	2583.5
7	6736.5
8	11347.0
9	11699.0
10	7658.5
11	4349.0
12	3980.0
13	4346.5
14	4816.5
15	5378.5
16	6132.0
17	6770.5
18	7541.5
19	8823.0
20	10597.5
21	12800.0
22	16158.5
23	21881.5
24	28950.5
25	37185.5
26	48331.0
27	61997.5
28	78557.5
29	96607.0
30	111978.5
31	125190.5
32	137768.5
33	148069.5
34	156903.0
35	165973.0
36	172836.0
37	178979.0
38	183856.0
39	185722.5
40	187073.0
41	187009.5
42	183667.5
43	180089.0
44	175710.5
45	170044.5
46	165022.5
47	157450.0
48	148225.0
49	142103.0
50	138544.0
51	131719.5
52	120949.0
53	111759.5
54	104726.5
55	98512.5
56	89435.5
57	77819.5
58	70530.0
59	65214.0
60	56823.0
61	46879.5
62	38623.5
63	31384.5
64	24667.5
65	19341.5
66	14788.0
67	11000.5
68	8410.5
69	6180.5
70	4308.5
71	3026.5
72	2304.5
73	1663.5
74	1126.0
75	802.5
76	492.5
77	324.0
78	221.0
79	112.0
80	53.5
81	24.5
82	13.5
83	8.0
84	7.0
85	4.5
86	2.0
87	2.5
88	3.5
89	3.5
90	4.0
91	2.0
92	0.0
93	2.0
94	5.5
95	8.5
96	13.5
97	16.0
98	8.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008929328121397772
2	1.9755150711057017E-5
3	4.543684663543114E-4
4	0.002666945345992697
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.0865332891081359E-4
24-25	5.9265452133171054E-5
26-27	4.543684663543114E-4
28-29	8.000836037978092E-4
30-31	0.001649555084373261
32-33	0.0040794386218332744
34-35	0.005244992513785639
36-37	0.003872009539367175
38-39	0.0024002508113934273
40-41	0.0034670289497905063
42-43	4.247357402877259E-4
44-45	4.1485816493219734E-4
46-47	0.0034966616758570924
48-49	0.0024693938388821272
50-51	9.383696587752083E-4
52-53	9.877575355528508E-6
54-55	3.852254388656118E-4
56-57	7.803284530867522E-4
58-59	7.013078502425241E-4
60-61	3.457151374434978E-4
62-63	0.0
64-65	0.0
66-67	6.22287247398296E-4
68-69	6.321648227538245E-4
70-71	0.0021039235507275722
72-73	7.902060284422807E-5
74-75	0.002015025372527816
76-77	3.457151374434978E-4
78-79	0.0010766557137526073
80-81	7.605733023756952E-4
82-83	0.0013828605497739912
84-85	0.0011951866180189496
86-87	2.271842331771557E-4
88-89	0.0014124932758405769
90-91	0.0015804120568845615
92-93	0.0027262107981258685
94-95	0.001066778138397079
96-97	0.0023804956606823706
98-99	0.0023311077839047283
100	0.003121313812347009
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5061971.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.87083647423561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.86404871856686	42.745742847171556
2	15.75998232319738	18.240867197251987
3	4.8500982352941495	8.420377255760593
4	2.1026591291446173	4.867305704951471
5	1.0291094710597628	2.9777712956893314
6	0.5876186289944735	2.040358895265222
7	0.3711631736600312	1.5035666329696584
8	0.2633195099804011	1.2190816242041314
9	0.184350917836172	0.9601687637975118
>10	0.8940062914460639	9.106235530832432
>50	0.06024187493918569	2.4063085031232365
>100	0.031759815090479065	3.3650931739341776
>500	0.0010261958442871792	0.39351649425646157
>1k	4.788894026275576E-4	0.5248782565232402
>5k	3.420638590166913E-5	0.1002841126733321
>10k+	1.0261915770500739E-4	1.1284437115955974
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24757	0.48907826615363853	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17980	0.3551976097848052	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14268	0.2818664903453615	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5066	0.10007959350221485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.9755150711057017E-5	0.0
3	0.0	0.0	0.0	1.9755150711057017E-5	0.0
4	0.0	0.0	0.0	7.902060284422807E-5	0.0
5	0.0	0.0	0.0	7.902060284422807E-5	0.0
6	0.0	0.0	0.0	7.902060284422807E-5	0.0
7	0.0	0.0	0.0	9.877575355528508E-5	0.0
8	0.0	0.0	0.0	1.5804120568845614E-4	0.0
9	0.0	0.0	0.0	1.9755150711057016E-4	0.0
10-11	0.0	0.0	0.0	1.9755150711057016E-4	0.0
12-13	0.0	0.0	0.0	2.3706180853268422E-4	0.0
14-15	0.0	0.0	0.0	3.0620483602138376E-4	0.0
16-17	0.0	0.0	0.0	3.358375620879693E-4	0.0
18-19	0.0	0.0	0.0	3.358375620879693E-4	0.0
20-21	0.0	0.0	0.0	3.457151374434978E-4	0.0
22-23	0.0	0.0	0.0	4.642460417098399E-4	0.0
24-25	0.0	0.0	0.0	7.309405763091096E-4	0.0
26-27	0.0	0.0	0.0	0.0010964108644636646	0.0
28-29	0.0	0.0	0.0	0.0022817199071270854	0.0
30-31	0.0	0.0	0.0	0.006242627624694018	0.0
32-33	0.0	0.0	0.0	0.0154880381574687	0.0
34-35	0.0	0.0	0.0	0.029257378203075446	0.0
36-37	0.0	0.0	0.0	0.04872607922882213	0.0
38-39	0.0	0.0	0.0	0.07548443086694887	0.0
40-41	0.0	0.0	0.0	0.10163037283303283	0.0
42-43	0.0	0.0	0.0	0.12877394991002516	0.0
44-45	0.0	0.0	0.0	0.16211076673493388	0.0
46-47	0.0	0.0	0.0	0.20429789107839613	0.0
48-49	0.0	0.0	0.0	0.24885563350718526	0.0
50-51	0.0	0.0	0.0	0.2944110110468827	0.0
52-53	0.0	0.0	0.0	0.33751674989840913	0.0
54-55	0.0	0.0	0.0	0.387023157580318	0.0
56-57	0.0	0.0	0.0	0.44732575512581957	0.0
58-59	0.0	0.0	0.0	0.5167552322998294	0.0
60-61	0.0	0.0	0.0	0.5919235807554014	0.0
62-63	0.0	0.0	0.0	0.6671314395123955	0.0
64-65	0.0	0.0	0.0	0.7478904956191965	0.0
66-67	0.0	0.0	0.0	0.8433868941564462	0.0
68-69	0.0	0.0	0.0	0.9553393332360063	0.0
70-71	0.0	0.0	0.0	1.0782361258094921	0.0
72-73	0.0	0.0	0.0	1.210072124079731	0.0
74-75	0.0	0.0	0.0	1.346491317314935	0.0
76-77	0.0	0.0	0.0	1.4945858046203742	0.0
78-79	0.0	0.0	0.0	1.6675421490956783	0.0
80-81	0.0	0.0	0.0	1.8629996102308763	0.0
82-83	0.0	0.0	0.0	2.0706756320808637	0.0
84-85	0.0	0.0	0.0	2.2811766404825313	0.0
86-87	0.0	0.0	0.0	2.5063260931364484	0.0
88	0.0	0.0	0.0	2.6945828018374662	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9605	0.0	59.30681	1
GTATCAA	19525	0.0	46.33818	1
ATCAACG	25070	0.0	35.693966	3
TCAACGC	25350	0.0	35.29971	4
TATCAAC	26175	0.0	34.402576	2
CAACGCA	26285	0.0	34.079803	5
AACGCAG	27485	0.0	32.626072	6
ACGCAGA	31255	0.0	28.630545	7
CGCAGAG	31600	0.0	28.213854	8
GTACATG	20255	0.0	26.47602	1
TACATGG	19700	0.0	26.29042	2
GTGGTAT	3535	0.0	25.926537	1
GCAGAGT	34365	0.0	25.738628	9
ACATGGG	20220	0.0	24.7543	3
TGGTATC	3610	0.0	23.694435	2
TACCTGG	3900	0.0	22.776098	2
AGAGTAC	32315	0.0	22.048407	10-11
GAGTACT	23125	0.0	21.451548	12-13
CATGGGG	10800	0.0	21.279787	4
AGTACTT	24115	0.0	20.892464	12-13
>>END_MODULE