FastQCFastQC Report
Wed 25 May 2016
SRR2431352_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431352_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310593
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA128850.557649053727766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.038.1131821
GTCTCGC8800.037.3861051
GTATCAA40400.033.0393751
TCTGTCG8800.032.045238
TACCTGG18900.031.581792
TCGCTCT10750.031.0416474
CTCGCTC9350.030.662893
TCTCGCT9550.030.512882
TCAACGC42500.030.0797944
GTACCTG21100.029.8481351
ATCAACG42400.029.8181923
CGCTCTG9300.029.3116265
CAACGCA45400.028.4689665
CTGTCGC10800.026.9812959
AACGCAG48350.026.7319776
TATCAAC49150.026.1056182
CGTTAGA950.001305037124.736672
CGCAGAG52900.023.81088
ACGCAGA54100.023.6301527
GTACATG74350.022.1881471
Produced by FastQC (version 0.11.5)