##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431352_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2310593 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.0676947432975 33.0 31.0 34.0 28.0 34.0 2 31.180403039392917 34.0 31.0 34.0 27.0 34.0 3 31.19107562430943 34.0 31.0 34.0 27.0 34.0 4 34.42156277630894 37.0 35.0 37.0 31.0 37.0 5 34.5247289332219 37.0 35.0 37.0 31.0 37.0 6 34.53748583155926 37.0 35.0 37.0 31.0 37.0 7 34.470785638145706 37.0 35.0 37.0 31.0 37.0 8 34.443602573019135 37.0 35.0 37.0 31.0 37.0 9 35.91780075504427 39.0 37.0 39.0 31.0 39.0 10-11 35.95381856519084 39.0 37.0 39.0 31.0 39.0 12-13 35.88672972695754 39.0 37.0 39.0 30.5 39.0 14-15 37.27412270356571 41.0 38.0 41.0 31.0 41.0 16-17 37.265029366920096 41.0 38.0 41.0 31.0 41.0 18-19 37.2769535785835 41.0 38.0 41.0 31.0 41.0 20-21 37.333222683527566 41.0 38.0 41.0 31.0 41.0 22-23 37.323713652728976 40.5 38.0 41.0 31.0 41.0 24-25 37.33944835806219 41.0 38.0 41.0 30.0 41.0 26-27 37.338682537339984 40.5 38.0 41.0 30.0 41.0 28-29 37.30019955916079 40.0 38.0 41.0 30.0 41.0 30-31 37.232548743980445 40.0 38.0 41.0 30.0 41.0 32-33 37.14892886804383 40.0 38.0 41.0 30.0 41.0 34-35 37.07894899707564 40.0 38.0 41.0 30.0 41.0 36-37 36.97814565351838 40.0 38.0 41.0 30.0 41.0 38-39 36.85555959011388 40.0 38.0 41.0 30.0 41.0 40-41 36.763210136964844 40.0 37.5 41.0 30.0 41.0 42-43 36.63293643666366 40.0 37.0 41.0 29.0 41.0 44-45 36.49117694029195 40.0 37.0 41.0 29.0 41.0 46-47 36.3541554051276 40.0 37.0 41.0 29.0 41.0 48-49 36.218235751601426 40.0 36.0 41.0 28.0 41.0 50-51 36.07072708174914 40.0 36.0 41.0 28.0 41.0 52-53 35.888925483631255 40.0 35.5 41.0 28.0 41.0 54-55 35.706815739509295 40.0 35.0 41.0 28.0 41.0 56-57 35.51296009292852 39.0 35.0 41.0 27.5 41.0 58-59 35.28224139863663 39.0 35.0 41.0 27.0 41.0 60-61 35.095317522384946 39.0 35.0 41.0 27.0 41.0 62-63 34.90533663868972 39.0 34.5 41.0 26.0 41.0 64-65 34.62559568041624 38.0 34.0 40.0 26.0 41.0 66-67 34.32941175706842 37.5 34.0 40.0 26.0 41.0 68-69 34.00199818834386 37.0 34.0 40.0 25.0 41.0 70-71 33.731364199579936 37.0 34.0 40.0 25.0 41.0 72-73 33.48284639484323 36.0 34.0 39.0 24.5 41.0 74-75 33.15140658696707 36.0 33.5 39.0 24.0 41.0 76-77 32.79795381531927 35.0 33.0 38.5 23.0 40.0 78-79 32.438722873305686 35.0 33.0 37.0 22.5 39.5 80-81 32.087223496305924 35.0 33.0 37.0 21.5 39.0 82-83 31.74962899134551 35.0 33.0 36.5 20.0 39.0 84-85 31.44096234170189 35.0 32.0 36.0 19.5 37.5 86-87 31.1570270056215 35.0 32.0 36.0 18.0 37.0 88-89 30.88828062752722 35.0 32.0 35.5 16.5 37.0 90-91 30.666555295545344 35.0 32.0 35.0 11.5 36.0 92-93 30.432666419399695 35.0 32.0 35.0 6.0 36.0 94-95 30.200094088400682 35.0 31.0 35.0 2.0 36.0 96-97 29.99493333529531 35.0 31.0 35.0 2.0 36.0 98-99 29.859964303535932 35.0 31.0 35.0 2.0 35.0 100 29.80522013180166 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.46696557607787526 1101 2 0.5194188980798273 1101 3 0.602703522619155 1101 4 0.5037418267997396 1101 5 0.46147165744239516 1101 6 0.4144901028236134 1101 7 0.5481921465227799 1101 8 0.5318541737052627 1101 9 0.6121217531376217 1101 10-11 0.5270277204891229 1101 12-13 0.6311675516819193 1101 14-15 0.739894522657444 1101 16-17 0.6752531350899389 1101 18-19 0.6320126265213162 1101 20-21 0.5302409116720455 1101 22-23 0.5853708921725698 1101 24-25 0.6207264008065323 1101 26-27 0.6295555318256376 1101 28-29 0.6239524852943958 1101 30-31 0.7051061334000366 1101 32-33 0.6563071884958376 1101 34-35 0.7057315820453169 1101 36-37 0.7000040362572477 1101 38-39 0.6309649235510193 1101 40-41 0.6219617355082718 1101 42-43 0.651908647779635 1101 44-45 0.6210349557725863 1101 46-47 0.6233573855117527 1101 48-49 0.6743757144596216 1101 50-51 0.6221710898650343 1101 52-53 0.6887735981081562 1101 54-55 0.6213728361976223 1101 56-57 0.6094405535437701 1101 58-59 0.6221103822450829 1101 60-61 0.674981286849679 1101 62-63 0.7088957472645774 1101 64-65 0.7300852453264071 1101 66-67 0.6755584892033895 1101 68-69 0.7408142779447076 1101 70-71 0.7283515687263318 1101 72-73 0.7136982096571813 1101 74-75 0.7294447710453511 1101 76-77 0.7774801503748421 1101 78-79 0.6752982539686698 1101 80-81 0.6311186674402265 1101 82-83 0.6684272812476273 1101 84-85 0.7110917574989308 1101 86-87 0.7466744967724281 1101 88-89 0.7386061216221194 1101 90-91 0.7168333830266747 1101 92-93 0.7047298454888562 1101 94-95 0.7217778516450295 1101 96-97 0.6671684639777702 1101 98-99 0.7336767141030123 1101 100 0.8353499906992958 1102 1 0.038538379258913125 1102 2 0.11560387951777784 1102 3 0.15053292139087304 1102 4 0.07869836163209243 1102 5 0.1093458908751117 1102 6 0.046717453545703336 1102 7 0.06350510391310138 1102 8 0.07454034272740984 1102 9 0.11464272804744269 1102 10-11 0.12581242872158072 1102 12-13 0.14419964076436287 1102 14-15 0.13231665625010436 1102 16-17 0.14365278607852616 1102 18-19 0.185962240526365 1102 20-21 0.13897930936771274 1102 22-23 0.07726758317819815 1102 24-25 0.12734281067755404 1102 26-27 0.14955687388098227 1102 28-29 0.15665338708049603 1102 30-31 0.1619875029280422 1102 32-33 0.15574013641667506 1102 34-35 0.20042396495701098 1102 36-37 0.271822578051534 1102 38-39 0.2345465650425922 1102 40-41 0.2538115007881814 1102 42-43 0.23405008866762955 1102 44-45 0.23990524554582038 1102 46-47 0.23828348630280516 1102 48-49 0.0938061823700096 1102 50-51 0.02804116677712898 1102 52-53 -0.02611338667954044 1102 54-55 0.05245574296627353 1102 56-57 0.0307466256317781 1102 58-59 -8.549573945657585E-4 1102 60-61 0.026944495278478087 1102 62-63 -0.03250857598283119 1102 64-65 -0.07121614400895027 1102 66-67 -0.08383707756613035 1102 68-69 -0.08415368938923962 1102 70-71 -0.0919380745439966 1102 72-73 -0.0660322875300352 1102 74-75 0.08912460516192056 1102 76-77 0.11376090296174368 1102 78-79 0.011349827018307224 1102 80-81 0.06900721003262333 1102 82-83 0.11420848598394784 1102 84-85 0.07990252605859638 1102 86-87 0.10332642682419646 1102 88-89 0.04078990753329492 1102 90-91 0.0550395132928827 1102 92-93 0.09751305358454943 1102 94-95 0.12778824747145023 1102 96-97 0.055340980282441166 1102 98-99 -0.005447847984040521 1102 100 -0.01310433027124347 1103 1 0.06540282643612372 1103 2 0.132889613323254 1103 3 0.17872896123540372 1103 4 0.11641026681685673 1103 5 0.15023049936413457 1103 6 0.144546587143509 1103 7 0.11387050629621598 1103 8 0.11682724791918986 1103 9 0.2118279498939799 1103 10-11 0.17720093533170456 1103 12-13 0.190514010545904 1103 14-15 0.30174779458787526 1103 16-17 0.27094572215619195 1103 18-19 0.20006264530696427 1103 20-21 0.2564894162570681 1103 22-23 0.21963100023689464 1103 24-25 0.21112849552329038 1103 26-27 0.23213441754984387 1103 28-29 0.15332777227103378 1103 30-31 0.08437074517381404 1103 32-33 0.16705222094279293 1103 34-35 0.23181683726075164 1103 36-37 0.26458467328145474 1103 38-39 0.24216829171930954 1103 40-41 0.23017474894279388 1103 42-43 0.09571511819919465 1103 44-45 0.06665412529368808 1103 46-47 0.016680204962192136 1103 48-49 0.06202724358708167 1103 50-51 -0.08182841514036454 1103 52-53 -0.0681787806174583 1103 54-55 -0.06858984626628484 1103 56-57 -0.0623192444360825 1103 58-59 0.030442240004092014 1103 60-61 0.0539091065058912 1103 62-63 0.01724644295153155 1103 64-65 0.04427398786810954 1103 66-67 0.06565524220801677 1103 68-69 0.0433750670923132 1103 70-71 0.08529603970418265 1103 72-73 0.0479760056044114 1103 74-75 0.10663535736587448 1103 76-77 0.06122696292045049 1103 78-79 0.027851643859506225 1103 80-81 -0.026743168809140627 1103 82-83 0.05331295203801645 1103 84-85 0.032977913114091706 1103 86-87 0.01796589376066038 1103 88-89 0.02076691749891424 1103 90-91 0.012232147840276042 1103 92-93 0.05125970811930003 1103 94-95 0.08114378523876908 1103 96-97 0.08611533256193482 1103 98-99 0.026543764048330587 1103 100 -0.005726139716571765 1104 1 0.12601955234631035 1104 2 0.10176366286486171 1104 3 0.1359436349285872 1104 4 0.07273466957208541 1104 5 0.116300056107562 1104 6 0.1153578727188318 1104 7 0.18439537329047795 1104 8 0.1310194414396193 1104 9 0.17362828755505433 1104 10-11 0.13304801873470495 1104 12-13 0.18743433655605912 1104 14-15 0.15680821578320803 1104 16-17 0.19906387296735062 1104 18-19 0.17989701517709733 1104 20-21 0.21631147893832292 1104 22-23 0.1317326347826011 1104 24-25 0.21622537080984472 1104 26-27 0.14789382234577886 1104 28-29 0.24227791242375218 1104 30-31 0.2346895723859177 1104 32-33 0.33152822596995435 1104 34-35 0.29866379660298037 1104 36-37 0.2051811808806221 1104 38-39 0.2577072189351952 1104 40-41 0.2529359013775334 1104 42-43 0.2536956874044307 1104 44-45 0.22905925925235948 1104 46-47 0.23257886348759627 1104 48-49 0.24358381080734404 1104 50-51 0.24692617645693815 1104 52-53 0.3592150530224316 1104 54-55 0.2431904888416554 1104 56-57 0.3575242322144163 1104 58-59 0.375975692363852 1104 60-61 0.38011447384266006 1104 62-63 0.3665963374998711 1104 64-65 0.4049864563359904 1104 66-67 0.425572463102732 1104 68-69 0.46822250829379186 1104 70-71 0.38844970066585205 1104 72-73 0.4400635799284984 1104 74-75 0.48032813433157173 1104 76-77 0.5606016696245888 1104 78-79 0.5313785659475414 1104 80-81 0.49650087672296905 1104 82-83 0.4546118144546121 1104 84-85 0.36813381615969476 1104 86-87 0.4259756352287809 1104 88-89 0.5362933487377788 1104 90-91 0.45146699849087923 1104 92-93 0.4255646980595884 1104 94-95 0.41669070448599044 1104 96-97 0.3953379479429522 1104 98-99 0.4221321447674846 1104 100 0.4315553829586811 1105 1 0.20728991183278822 1105 2 0.28862814794876357 1105 3 0.29084205037624855 1105 4 0.24451112195761482 1105 5 0.2622223612271881 1105 6 0.32512154867733756 1105 7 0.3210051270018397 1105 8 0.3014731386680083 1105 9 0.3257661468694195 1105 10-11 0.26210505972000675 1105 12-13 0.36834633284559715 1105 14-15 0.3385807910340475 1105 16-17 0.37016517851092345 1105 18-19 0.32205324558760395 1105 20-21 0.2927449317260624 1105 22-23 0.28618635737543485 1105 24-25 0.3319367344379671 1105 26-27 0.30871447044326317 1105 28-29 0.2986717423443679 1105 30-31 0.26589045144467605 1105 32-33 0.2798462017432968 1105 34-35 0.2791961416897024 1105 36-37 0.2671136064088415 1105 38-39 0.33158103813026685 1105 40-41 0.333015672517881 1105 42-43 0.275790191656597 1105 44-45 0.24682079372806243 1105 46-47 0.2770666287723813 1105 48-49 0.221609697022501 1105 50-51 0.22386193559628964 1105 52-53 0.20213339404359942 1105 54-55 0.20303940741095516 1105 56-57 0.1553583814369972 1105 58-59 0.09652631795945865 1105 60-61 0.15072769523430196 1105 62-63 0.1533540202053132 1105 64-65 0.11002220206842139 1105 66-67 0.1193614382709498 1105 68-69 0.06909078717210093 1105 70-71 0.13690029618648936 1105 72-73 0.16137142442278218 1105 74-75 0.15532378614312847 1105 76-77 0.20199234710858605 1105 78-79 0.05848209269070992 1105 80-81 0.07483240435026772 1105 82-83 0.13263117838711125 1105 84-85 0.1402297698080055 1105 86-87 0.14205396784345936 1105 88-89 0.06885521077700574 1105 90-91 0.09616347682059256 1105 92-93 0.062427643160084756 1105 94-95 0.11230682466991837 1105 96-97 0.15638370537925894 1105 98-99 0.17028651995092403 1105 100 0.11878692761638732 1106 1 0.05962418476866205 1106 2 0.09513407575038713 1106 3 0.07575470682585816 1106 4 0.06519448452516485 1106 5 0.09940243711476171 1106 6 0.07039814821705903 1106 7 0.10523222874271454 1106 8 0.08331509550121297 1106 9 0.10667508212534926 1106 10-11 0.09493702018522043 1106 12-13 0.06610920852634905 1106 14-15 0.0927256057862067 1106 16-17 0.07256797287821115 1106 18-19 0.08312168634549977 1106 20-21 0.1364335224141584 1106 22-23 0.12438889351937377 1106 24-25 0.10203643941797935 1106 26-27 0.15840557937055166 1106 28-29 0.18029969309743166 1106 30-31 0.11721775207811902 1106 32-33 0.12096285599123036 1106 34-35 0.1086770024931667 1106 36-37 0.19638939649021836 1106 38-39 0.12433323733462487 1106 40-41 0.05132331910796495 1106 42-43 0.1162606067712062 1106 44-45 0.08819640316202992 1106 46-47 0.0448728400886651 1106 48-49 0.06752183028562087 1106 50-51 -0.028310888184492455 1106 52-53 0.04208530309561809 1106 54-55 0.06438035105965412 1106 56-57 0.04988660644599463 1106 58-59 0.05058683399085595 1106 60-61 -0.05293158067151893 1106 62-63 0.009009693374771643 1106 64-65 -0.06046459964185402 1106 66-67 -0.07997746437177256 1106 68-69 0.04181113905841727 1106 70-71 0.05636744589578058 1106 72-73 -0.010748590540956116 1106 74-75 -0.027433985320712395 1106 76-77 0.04362716702452474 1106 78-79 0.07434984190295069 1106 80-81 -0.017654423229998883 1106 82-83 -0.07637433498409862 1106 84-85 -0.01637574043157386 1106 86-87 -0.06786397323219617 1106 88-89 -0.143310951481606 1106 90-91 -0.11032209538441862 1106 92-93 -0.09713010937781164 1106 94-95 -0.06007350705822745 1106 96-97 -0.031832718864048815 1106 98-99 -0.034483463509300094 1106 100 -0.09561520161986437 1107 1 0.027661527794705876 1107 2 0.04967260426088416 1107 3 0.04703859248642672 1107 4 0.10249520167983661 1107 5 0.06638600774500958 1107 6 0.02711691381228576 1107 7 0.0361266963958613 1107 8 -0.024158598927733976 1107 9 -0.04123520054513108 1107 10-11 0.008157499936125134 1107 12-13 -0.05162902508283196 1107 14-15 0.02272471251414032 1107 16-17 -0.04501682454272071 1107 18-19 -0.012733622417485435 1107 20-21 -0.043352824529975464 1107 22-23 -0.0016872296677021836 1107 24-25 -0.029948002456364975 1107 26-27 -0.0010456492659187688 1107 28-29 0.07768772303906246 1107 30-31 0.0621093980245746 1107 32-33 0.10950390723572667 1107 34-35 0.02330503139567952 1107 36-37 0.04030487684955375 1107 38-39 0.04863883603883323 1107 40-41 0.061355159395780845 1107 42-43 0.048898284227377076 1107 44-45 0.04110991330955471 1107 46-47 0.07944797604299936 1107 48-49 0.0304422663299988 1107 50-51 -0.06978757629019583 1107 52-53 -0.02292006489221876 1107 54-55 -0.03517569424261069 1107 56-57 -0.07611127947678398 1107 58-59 -0.05165844636516681 1107 60-61 -0.1417028089859329 1107 62-63 -0.04891987364197803 1107 64-65 -0.0907245326981041 1107 66-67 0.008963986961383341 1107 68-69 -0.006375305963345568 1107 70-71 -0.014726224466471649 1107 72-73 -0.041841902379047724 1107 74-75 0.01902630258331328 1107 76-77 -0.0070017998136648885 1107 78-79 -0.0571045614953718 1107 80-81 -0.08245196873711791 1107 82-83 -0.06443096083724598 1107 84-85 -0.09413571944272192 1107 86-87 -0.08794246571709152 1107 88-89 -0.1231712364384343 1107 90-91 -0.08623253787159868 1107 92-93 -0.0643194895882786 1107 94-95 -0.024874068118158732 1107 96-97 0.04743575815721002 1107 98-99 0.06399997168692195 1107 100 0.03461535828093787 1108 1 -0.041491037106272444 1108 2 -0.03481440807638947 1108 3 0.0032586024327088126 1108 4 -0.08917725204979376 1108 5 -0.1799491759550378 1108 6 -0.09899043135204266 1108 7 -0.16783568694815187 1108 8 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74-75 -0.2826040774051677 2214 76-77 -0.2845333660943936 2214 78-79 -0.22908930811983197 2214 80-81 -0.247625318756306 2214 82-83 -0.16218751119764363 2214 84-85 -0.2490148062161417 2214 86-87 -0.216765307377635 2214 88-89 -0.23473254657819353 2214 90-91 -0.2193577934568296 2214 92-93 -0.19454597865985335 2214 94-95 -0.13744526480581243 2214 96-97 -0.20746968167336277 2214 98-99 -0.18840506747531194 2214 100 -0.22765065716682287 2215 1 -0.20960793507842723 2215 2 -0.2254373232789888 2215 3 -0.18045501164534983 2215 4 -0.22239565182951537 2215 5 -0.13908776335453865 2215 6 -0.15274056662842384 2215 7 -0.1896859390759289 2215 8 -0.1938704554810542 2215 9 -0.24772844642195935 2215 10-11 -0.24497219513746415 2215 12-13 -0.20451386122452675 2215 14-15 -0.2365224820974987 2215 16-17 -0.2451928552245235 2215 18-19 -0.23836370476131208 2215 20-21 -0.1994748699115405 2215 22-23 -0.1603601544113502 2215 24-25 -0.26056542666427873 2215 26-27 -0.27876150643558617 2215 28-29 -0.2912748606608062 2215 30-31 -0.3231215160955685 2215 32-33 -0.3829987053396664 2215 34-35 -0.4856871403671832 2215 36-37 -0.4174822901143145 2215 38-39 -0.39418544891915985 2215 40-41 -0.42640747782652255 2215 42-43 -0.2946221348315703 2215 44-45 -0.323736335437772 2215 46-47 -0.30610844376712265 2215 48-49 -0.3072283338330237 2215 50-51 -0.34613536408131296 2215 52-53 -0.32585754306651893 2215 54-55 -0.3031600891531525 2215 56-57 -0.3173477530230926 2215 58-59 -0.2983795982949289 2215 60-61 -0.3663991264279005 2215 62-63 -0.4683297758799654 2215 64-65 -0.4000087894657014 2215 66-67 -0.3620388822983003 2215 68-69 -0.5209448543202626 2215 70-71 -0.5707588328605198 2215 72-73 -0.46775633459976973 2215 74-75 -0.4882450944143528 2215 76-77 -0.49518157898364734 2215 78-79 -0.504404601075386 2215 80-81 -0.42290344670492175 2215 82-83 -0.4247339010559408 2215 84-85 -0.4156470620245436 2215 86-87 -0.44388029995391776 2215 88-89 -0.45537089509252127 2215 90-91 -0.4251111950917732 2215 92-93 -0.35068748805577954 2215 94-95 -0.2505923268086505 2215 96-97 -0.22969876544489765 2215 98-99 -0.26407304079458527 2215 100 -0.27067931275037793 2216 1 -0.16120692202977693 2216 2 -0.20749755758967936 2216 3 -0.3347254999104905 2216 4 -0.3264692861533547 2216 5 -0.27298920998273957 2216 6 -0.18169102819147298 2216 7 -0.20194250034574424 2216 8 -0.3178949636320638 2216 9 -0.34885383921943003 2216 10-11 -0.2873461603290437 2216 12-13 -0.32436125887098655 2216 14-15 -0.36580152450129333 2216 16-17 -0.34776229102921263 2216 18-19 -0.40763221125033766 2216 20-21 -0.3527769352217831 2216 22-23 -0.3491221850476549 2216 24-25 -0.3910020276640793 2216 26-27 -0.44296787687944317 2216 28-29 -0.3541506694821166 2216 30-31 -0.3939645744664304 2216 32-33 -0.5157307238442002 2216 34-35 -0.4891937779189135 2216 36-37 -0.47079762940942516 2216 38-39 -0.46493501145560145 2216 40-41 -0.48707555450192785 2216 42-43 -0.5160054182859568 2216 44-45 -0.33699767397497027 2216 46-47 -0.3274357167226327 2216 48-49 -0.4327704325618882 2216 50-51 -0.3483660584809911 2216 52-53 -0.36713559663301254 2216 54-55 -0.38982305285863106 2216 56-57 -0.2793474912720413 2216 58-59 -0.305047445285652 2216 60-61 -0.31876978803786216 2216 62-63 -0.3755430369885744 2216 64-65 -0.40477524376932905 2216 66-67 -0.3855098168079678 2216 68-69 -0.3866818842512316 2216 70-71 -0.465788241324276 2216 72-73 -0.4839737448229471 2216 74-75 -0.37757576242763946 2216 76-77 -0.46560625825470936 2216 78-79 -0.3785540189154446 2216 80-81 -0.38160827243228823 2216 82-83 -0.3702430435130175 2216 84-85 -0.3812207088393542 2216 86-87 -0.3354286121008698 2216 88-89 -0.4109423519483997 2216 90-91 -0.38218483078278354 2216 92-93 -0.5309221020096473 2216 94-95 -0.5705906290311873 2216 96-97 -0.6668534693884283 2216 98-99 -0.6796796737103641 2216 100 -0.6676710151426661 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48430.0 3 9439.0 4 6628.0 5 6313.0 6 4461.0 7 4148.0 8 4683.0 9 4986.0 10 5241.0 11 5838.0 12 6990.0 13 7320.0 14 7553.0 15 7698.0 16 7237.0 17 7024.0 18 6844.0 19 6777.0 20 6950.0 21 7189.0 22 8586.0 23 13180.0 24 20715.0 25 24772.0 26 23447.0 27 18937.0 28 19203.0 29 23496.0 30 27510.0 31 33708.0 32 43044.0 33 57426.0 34 81671.0 35 126744.0 36 216280.0 37 429918.0 38 652248.0 39 316323.0 40 1636.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.483478245816 21.614998766548805 13.34040223492701 22.561120752708185 2 16.228344844808237 22.327818010354918 39.5535258697659 21.890311275070946 3 17.190738481420137 27.868127359513338 30.519481362576624 24.421652796489905 4 12.47939381795063 19.469547427868083 38.944418164514474 29.10664058966681 5 11.916854244776125 38.54439098534446 34.11821121244633 15.420543557433092 6 27.62485647623792 40.7597097368511 18.565710187817587 13.049723599093394 7 24.324145360087215 34.41925947148633 23.480336000325458 17.776259168101003 8 22.96492718535891 36.49703777342007 22.363782803808373 18.174252237412645 9 23.464582468656314 18.802489231119456 22.33318459806638 35.39974370215784 10-11 21.800269454637835 28.80537593596103 29.470724614849953 19.92362999455118 12-13 23.2002780238666 27.002721812106245 28.69769795026645 21.09930221376071 14-15 21.309313236904984 28.84041023235161 26.294094200060332 23.556182330683075 16-17 20.45422971505583 31.769333673217222 26.203121882564346 21.5733147291626 18-19 20.038513922616403 30.147217619026804 28.60127248719268 21.212995971164112 20-21 22.37516516322866 29.67712184707562 27.23281426023536 20.71489872946036 22-23 21.50911043182421 30.35805094190106 27.457518481186433 20.675320145088296 24-25 21.482905037797657 29.61625002759032 27.69535785835065 21.205487076261377 26-27 21.183111867819214 29.288628503591934 27.926099490477117 21.60216013811173 28-29 21.156928113259237 29.467370497530286 27.85416990357021 21.521531485640267 30-31 21.807756710073996 30.43259890426397 26.991166336953327 20.768478048708708 32-33 20.613128318141708 29.786942139961475 28.30409769267024 21.295831849226584 34-35 20.719269901709215 28.760625519076704 28.356962909521492 22.163141669692585 36-37 21.980504571770105 30.071955554266804 27.075062548878144 20.872477325084944 38-39 21.3894874605783 29.757642302214194 27.802754530979712 21.050115706227796 40-41 20.80680154401922 29.375857193369843 28.093459124995185 21.723882137615753 42-43 20.619360484516314 29.60302831351086 28.078679369322074 21.698931832650754 44-45 21.49749003827156 29.473256432439637 27.84776462146297 21.181488907825827 46-47 21.100297629223324 30.602403798505406 27.283602088295083 21.01369648397619 48-49 20.65601774090028 29.60263880311245 28.272958500263783 21.468384955723486 50-51 20.935816043760195 29.026249971327704 28.18700653901401 21.850927445898087 52-53 20.919543164893167 29.3735850493791 27.927138184872884 21.779733600854843 54-55 21.29935908227888 30.234684342937072 27.44951187855239 21.01644469623166 56-57 20.77860531906744 29.75132357797327 27.545417994428274 21.924653108531015 58-59 20.411794721095408 28.983338909102557 28.373192509455365 22.231673860346675 60-61 21.713863064589916 29.914766469040632 27.10094335090602 21.270427115463434 62-63 21.210464153574428 29.791897577807948 27.478249090168628 21.519389178448993 64-65 21.004431333801612 29.105432333113047 28.231391881990366 21.65874445109498 66-67 20.906602763879228 29.44770022241044 27.43784820606658 22.207848807643753 68-69 21.89195587453091 29.664181446061683 27.290158846668366 21.15370383273904 70-71 22.0351442248808 29.173658017660404 27.618299717864637 21.172898039594166 72-73 21.236777744933878 29.42673157929588 28.400544795210582 20.935945880559668 74-75 21.168094078013738 29.058341300263614 28.164869364704217 21.608695257018436 76-77 21.48647554978311 29.522572776772023 27.455852242259887 21.53509943118498 78-79 21.71262961499494 29.69261570514582 27.6242722106403 20.97048246921894 80-81 21.366143099659503 29.149709435999643 27.90260506774778 21.581542396593083 82-83 20.823062685950923 29.38873391400766 28.16188249795128 21.62632090209014 84-85 21.330995095586722 29.27824798361618 27.690719008107084 21.700037912690018 86-87 21.666796866462402 29.492331159898406 27.558463855923577 21.28240811771562 88-89 20.925621158167974 29.253658024607198 27.92336299138391 21.897357825840917 90-91 20.91948192581016 28.980811017094215 27.755782955211135 22.34392410188449 92-93 21.304974539452 29.683508433434586 27.472505832522376 21.539011194591037 94-95 21.136779271827127 29.229120596017417 27.795838758660597 21.83826137349486 96-97 20.923441139905457 29.390024204272375 27.904507274806672 21.7820273810155 98-99 20.7913434448815 29.223945278273156 27.96172214428183 22.02298913256351 100 21.54935825717754 29.49731768596043 27.428679011731198 21.52464504513083 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 10469.0 1 6994.0 2 2867.0 3 1830.0 4 1231.0 5 937.0 6 811.0 7 837.5 8 911.0 9 908.5 10 794.0 11 655.5 12 636.0 13 683.5 14 732.0 15 776.0 16 919.0 17 1178.5 18 1569.0 19 2136.0 20 2986.5 21 4148.5 22 5870.5 23 8684.5 24 12134.0 25 16030.5 26 21133.0 27 27510.5 28 35217.0 29 43138.0 30 49816.5 31 56014.0 32 61157.5 33 65274.5 34 69413.5 35 72484.0 36 75009.0 37 77026.0 38 78492.5 39 79242.5 40 79452.0 41 78349.0 42 75617.0 43 73037.0 44 70362.5 45 68015.5 46 66600.0 47 65634.0 48 65568.0 49 66427.5 50 66198.5 51 63859.5 52 59887.0 53 56361.5 54 53715.5 55 51037.0 56 48394.0 57 45710.5 58 42943.5 59 39990.5 60 35740.0 61 30634.0 62 25666.0 63 20780.5 64 16694.5 65 13393.5 66 10146.0 67 7061.0 68 4781.0 69 3370.0 70 2548.0 71 1961.5 72 1577.5 73 1251.0 74 945.0 75 712.5 76 547.0 77 418.5 78 315.0 79 248.0 80 191.5 81 136.5 82 111.5 83 111.0 84 96.5 85 78.0 86 72.5 87 69.0 88 57.0 89 45.0 90 37.5 91 30.5 92 29.0 93 24.0 94 13.5 95 10.5 96 12.0 97 11.5 98 11.5 99 13.0 100 31.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.2983679947095833E-4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.655786631397221E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.1639466578493053E-5 82-83 1.5147626604945137E-4 84-85 9.088575962967083E-4 86-87 0.0017960757260149234 88-89 0.003916743450707243 90-91 0.003224280520195465 92-93 0.0024885386565267013 94-95 0.0024452597233697152 96-97 0.0021855861244277986 98-99 0.002878049054939576 100 0.0038085461178147774 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2310593.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.64277613624623 #Duplication Level Percentage of deduplicated Percentage of total 1 84.80051676254949 63.29745988944977 2 10.480037179655731 15.645181382011597 3 2.3677409100767854 5.302042640984801 4 0.8570249422883027 2.558828836416205 5 0.4194531224865743 1.5654572760707415 6 0.25203072763319123 1.1287363909307724 7 0.16784219094921962 0.8769744959667783 8 0.1193706351266163 0.7128124475998039 9 0.08134196871592522 0.5464431325209915 >10 0.42287702343752387 5.593775429083865 >50 0.023391520485932793 1.1989209340330576 >100 0.00814107875348212 0.9183060179769362 >500 1.7395338091352337E-4 0.09738272452252145 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 5.7984460304473654E-5 0.5576784024320536 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12885 0.557649053727766 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.3278933156986105E-5 0.0 12-13 0.0 0.0 0.0 8.655786631397221E-5 0.0 14-15 0.0 0.0 0.0 1.2983679947095833E-4 0.0 16-17 0.0 0.0 0.0 1.7311573262794442E-4 0.0 18-19 0.0 0.0 0.0 1.7311573262794442E-4 0.0 20-21 0.0 0.0 0.0 2.3803413236342358E-4 0.0 22-23 0.0 0.0 0.0 3.89510398412875E-4 0.0 24-25 0.0 0.0 0.0 6.924629305117777E-4 0.0 26-27 0.0 0.0 0.0 0.0011252522620816387 0.0 28-29 0.0 0.0 0.0 0.002466899189948208 0.0 30-31 0.0 0.0 0.0 0.0057993770430361385 0.0 32-33 0.0 0.0 0.0 0.013459748211822678 0.0 34-35 2.1639466578493053E-5 0.0 0.0 0.02663818335812495 0.0 36-37 4.3278933156986105E-5 0.0 0.0 0.045161566749315 0.0 38-39 6.491839973547916E-5 0.0 0.0 0.06922465358459928 0.0 40-41 8.655786631397221E-5 0.0 0.0 0.09696644973822738 0.0 42-43 8.655786631397221E-5 0.0 0.0 0.1264826821512919 0.0 44-45 8.655786631397221E-5 0.0 0.0 0.16065139987873245 0.0 46-47 8.655786631397221E-5 0.0 0.0 0.2018745837107617 0.0 48-49 8.655786631397221E-5 0.0 0.0 0.2467764768611348 0.0 50-51 8.655786631397221E-5 0.0 0.0 0.29585478706115703 0.0 52-53 8.655786631397221E-5 0.0 0.0 0.345668839124848 0.0 54-55 8.655786631397221E-5 0.0 0.0 0.40104423409921175 0.0 56-57 8.655786631397221E-5 0.0 0.0 0.46736919916229297 0.0 58-59 8.655786631397221E-5 0.0 0.0 0.5408351881962769 0.0 60-61 8.655786631397221E-5 0.0 0.0 0.6152100348265575 0.0 62-63 8.655786631397221E-5 0.0 0.0 0.6893901262576316 0.0 64-65 8.655786631397221E-5 0.0 0.0 0.7780902997628747 0.0 66-67 8.655786631397221E-5 0.0 0.0 0.8798174321483706 0.0 68-69 8.655786631397221E-5 0.0 0.0 0.9915203586265517 0.0 70-71 8.655786631397221E-5 0.0 0.0 1.1157741757202588 0.0 72-73 8.655786631397221E-5 0.0 0.0 1.264740263646605 0.0 74-75 8.655786631397221E-5 0.0 0.0 1.4146801275689833 0.0 76-77 8.655786631397221E-5 0.0 0.0 1.5784908895681757 0.0 78-79 8.655786631397221E-5 0.0 0.0 1.7630971789493 0.0 80-81 8.655786631397221E-5 0.0 0.0 1.9665947226534486 0.0 82-83 8.655786631397221E-5 0.0 0.0 2.1846772668315015 0.0 84-85 8.655786631397221E-5 0.0 0.0 2.4051185128666104 0.0 86-87 8.655786631397221E-5 0.0 0.0 2.6449270814894703 0.0 88 8.655786631397221E-5 0.0 0.0 2.8426468876171613 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2380 0.0 38.113182 1 GTCTCGC 880 0.0 37.386105 1 GTATCAA 4040 0.0 33.039375 1 TCTGTCG 880 0.0 32.04523 8 TACCTGG 1890 0.0 31.58179 2 TCGCTCT 1075 0.0 31.041647 4 CTCGCTC 935 0.0 30.66289 3 TCTCGCT 955 0.0 30.51288 2 TCAACGC 4250 0.0 30.079794 4 GTACCTG 2110 0.0 29.848135 1 ATCAACG 4240 0.0 29.818192 3 CGCTCTG 930 0.0 29.311626 5 CAACGCA 4540 0.0 28.468966 5 CTGTCGC 1080 0.0 26.981295 9 AACGCAG 4835 0.0 26.731977 6 TATCAAC 4915 0.0 26.105618 2 CGTTAGA 95 0.0013050371 24.73667 2 CGCAGAG 5290 0.0 23.8108 8 ACGCAGA 5410 0.0 23.630152 7 GTACATG 7435 0.0 22.188147 1 >>END_MODULE