FastQCFastQC Report
Wed 25 May 2016
SRR2431352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431352_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310593
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117940.5104317376534941No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92570.40063308423422034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67830.29356100360383675No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44080.19077353735599475No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24260.10499469183884831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45000.059.0159651
GTATCAA89050.045.8690151
ATCAACG119650.034.0166663
TATCAAC121650.033.457412
TCAACGC122200.033.3837474
CAACGCA124350.032.8436285
TATAACG2300.032.69482
GTCTCGC11550.031.742871
TACCTGG20050.031.6450612
AACGCAG129550.031.6341536
TAGATCG2051.8189894E-1229.8034576
CGCTCTG10650.029.5666685
ATAACGC2550.029.489433
GTACCTG21800.029.3235111
TCGCTCT13850.028.5046714
ACGCAGA144750.028.1824237
CGCAGAG144600.028.081658
TGGTATC17950.026.7068272
TCTGTCG8300.026.0469518
GCAGAGT155550.025.9537629
Produced by FastQC (version 0.11.5)