##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431352_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2310593 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75541343715661 34.0 31.0 34.0 31.0 34.0 2 32.89150317689009 34.0 31.0 34.0 31.0 34.0 3 32.96176609208112 34.0 31.0 34.0 31.0 34.0 4 36.350090214936166 37.0 37.0 37.0 35.0 37.0 5 36.30754788922151 37.0 37.0 37.0 35.0 37.0 6 36.33949856162466 37.0 37.0 37.0 35.0 37.0 7 36.20589259986506 37.0 37.0 37.0 35.0 37.0 8 36.26463639420703 37.0 37.0 37.0 35.0 37.0 9 38.08245632181868 39.0 39.0 39.0 35.0 39.0 10-11 38.07926514968236 39.0 39.0 39.0 36.0 39.0 12-13 38.057857441790915 39.0 39.0 39.0 35.0 39.0 14-15 39.55711845400727 41.0 40.0 41.0 37.0 41.0 16-17 39.56302970709251 41.0 40.0 41.0 37.0 41.0 18-19 39.63218771112005 41.0 40.0 41.0 37.0 41.0 20-21 39.62660040084948 41.0 40.0 41.0 37.0 41.0 22-23 39.534822229618115 41.0 40.0 41.0 36.5 41.0 24-25 39.49768046557745 41.0 40.0 41.0 36.0 41.0 26-27 39.39997178213558 41.0 39.0 41.0 36.0 41.0 28-29 39.298163285355756 41.0 39.0 41.0 36.0 41.0 30-31 39.12704639025566 41.0 39.0 41.0 35.0 41.0 32-33 38.89992720483443 41.0 39.0 41.0 35.0 41.0 34-35 38.76885803774183 41.0 38.5 41.0 35.0 41.0 36-37 38.63759151871403 41.0 38.0 41.0 35.0 41.0 38-39 38.494246282231444 41.0 38.0 41.0 35.0 41.0 40-41 38.38476075189356 41.0 38.0 41.0 35.0 41.0 42-43 38.24818953402871 41.0 38.0 41.0 34.5 41.0 44-45 38.05017694591821 41.0 38.0 41.0 34.0 41.0 46-47 37.752266625926765 40.5 38.0 41.0 33.0 41.0 48-49 37.486568383094735 40.0 38.0 41.0 33.0 41.0 50-51 37.340517780500505 40.0 38.0 41.0 33.0 41.0 52-53 37.21950230958027 40.0 37.5 41.0 32.5 41.0 54-55 37.06881372011428 40.0 37.0 41.0 32.0 41.0 56-57 36.912447800196745 40.0 37.0 41.0 32.0 41.0 58-59 36.73387264654571 40.0 36.0 41.0 31.0 41.0 60-61 36.53149884034099 40.0 36.0 41.0 31.0 41.0 62-63 36.280672537309684 39.0 35.0 41.0 31.0 41.0 64-65 35.99151235202392 39.0 35.0 41.0 30.5 41.0 66-67 35.71374729344372 39.0 35.0 41.0 30.0 41.0 68-69 35.43741758068167 38.0 35.0 40.5 30.0 41.0 70-71 35.16523961597737 37.0 35.0 40.0 30.0 41.0 72-73 34.9064707631331 37.0 35.0 39.5 30.0 41.0 74-75 34.61199873798631 36.0 35.0 39.0 30.0 41.0 76-77 34.289892681229446 36.0 35.0 39.0 29.5 41.0 78-79 33.9645041337873 35.5 35.0 38.0 29.0 40.0 80-81 33.648152443982994 35.0 35.0 37.0 29.0 39.0 82-83 33.326392835086054 35.0 34.0 37.0 29.0 39.0 84-85 33.04521588181043 35.0 34.0 36.0 29.0 38.5 86-87 32.80803109851021 35.0 34.0 36.0 29.0 37.0 88-89 32.58003010482591 35.0 34.0 36.0 29.0 37.0 90-91 32.372518440071445 35.0 34.0 35.5 28.0 36.5 92-93 32.21395503232287 35.0 34.0 35.0 27.0 36.0 94-95 32.06909395120647 35.0 34.0 35.0 27.0 36.0 96-97 31.923837733430336 35.0 34.0 35.0 27.0 36.0 98-99 31.79938158732412 35.0 34.0 35.0 27.0 36.0 100 31.692778866723824 35.0 34.0 35.0 26.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2351470023274942 1101 2 0.26532211836063624 1101 3 0.24611529574638524 1101 4 0.16784806740574254 1101 5 0.1766901038175206 1101 6 0.21068580999050113 1101 7 0.20253626696723614 1101 8 0.20218997994117416 1101 9 0.23427770918400626 1101 10-11 0.29969790206821756 1101 12-13 0.31226180880524623 1101 14-15 0.37398618292843366 1101 16-17 0.42021952659604267 1101 18-19 0.37242235507044086 1101 20-21 0.3778448806889898 1101 22-23 0.4041839314912252 1101 24-25 0.3969208239843738 1101 26-27 0.4117432866910846 1101 28-29 0.4273799333187398 1101 30-31 0.4210476598973045 1101 32-33 0.42159490968342794 1101 34-35 0.4579270291254858 1101 36-37 0.45425641024780106 1101 38-39 0.45601707142783 1101 40-41 0.4810056438412431 1101 42-43 0.4950185673967198 1101 44-45 0.5457150553698895 1101 46-47 0.5411223396579175 1101 48-49 0.5396739053728936 1101 50-51 0.47506988366843217 1101 52-53 0.507890517220261 1101 54-55 0.5206716366320521 1101 56-57 0.5134368297318517 1101 58-59 0.6117665606638241 1101 60-61 0.5248417335629298 1101 62-63 0.5144591407913524 1101 64-65 0.5544487609139424 1101 66-67 0.6000361334068458 1101 68-69 0.54198214142599 1101 70-71 0.5187722016893588 1101 72-73 0.5216791952645607 1101 74-75 0.5103247533366257 1101 76-77 0.5078412348886587 1101 78-79 0.5006694040920436 1101 80-81 0.4974236334051838 1101 82-83 0.5446774837483517 1101 84-85 0.5595304231891873 1101 86-87 0.49251366245127315 1101 88-89 0.4843613203984418 1101 90-91 0.5263743455946681 1101 92-93 0.5367873579745321 1101 94-95 0.5381593441529802 1101 96-97 0.5598771346342737 1101 98-99 0.5799614169909937 1101 100 0.5785456674230254 1102 1 0.17054176732965232 1102 2 0.16942436949962314 1102 3 0.2077809385422782 1102 4 0.1554463784955189 1102 5 0.19188931383406072 1102 6 0.1703704289098411 1102 7 0.21623697687985555 1102 8 0.19479317547700248 1102 9 0.2779242564770712 1102 10-11 0.24048442737043274 1102 12-13 0.2540230571593298 1102 14-15 0.332699159554231 1102 16-17 0.3672175827338151 1102 18-19 0.3378324670627464 1102 20-21 0.3155516585402296 1102 22-23 0.30076768821221833 1102 24-25 0.357009022244263 1102 26-27 0.32178774299644175 1102 28-29 0.2930121294767858 1102 30-31 0.27734578411021005 1102 32-33 0.3044226472524869 1102 34-35 0.3400508615541824 1102 36-37 0.2115407429646936 1102 38-39 0.2725423649831811 1102 40-41 0.2574440138004519 1102 42-43 0.21366364410905447 1102 44-45 0.17876857882837527 1102 46-47 0.16699187166089047 1102 48-49 0.19233607047400625 1102 50-51 0.15822654995289298 1102 52-53 0.17212247021019778 1102 54-55 0.20758288085117726 1102 56-57 0.23541663478339103 1102 58-59 0.24512912269176468 1102 60-61 0.22489168998464493 1102 62-63 0.2125886294923376 1102 64-65 0.27624656054007346 1102 66-67 0.2543309078727134 1102 68-69 0.2656995423095765 1102 70-71 0.2394832638164175 1102 72-73 0.23439284946464056 1102 74-75 0.2006678179190473 1102 76-77 0.16830806797403852 1102 78-79 0.14837973155451323 1102 80-81 0.11339849172158978 1102 82-83 0.11591072959777904 1102 84-85 0.12796739949035896 1102 86-87 0.19805877712431652 1102 88-89 0.20557849335404654 1102 90-91 0.18582472098025704 1102 92-93 0.1938850448737952 1102 94-95 0.21336086666374143 1102 96-97 0.19517956353301358 1102 98-99 0.2075133799972484 1102 100 0.2685050813203027 1103 1 0.19174876938595986 1103 2 0.20470371917961927 1103 3 0.18355807268887503 1103 4 0.1361441768223557 1103 5 0.1662649929926161 1103 6 0.1534401792825122 1103 7 0.14363599706782537 1103 8 0.1598774465506665 1103 9 0.232126839811734 1103 10-11 0.2417359168547506 1103 12-13 0.23354792604122565 1103 14-15 0.31268669095369717 1103 16-17 0.3300136523750439 1103 18-19 0.28764592905498176 1103 20-21 0.30567413644732255 1103 22-23 0.33357211608049653 1103 24-25 0.3189266223023992 1103 26-27 0.3466533983148352 1103 28-29 0.35198939560424947 1103 30-31 0.38913419498931745 1103 32-33 0.32925617712620436 1103 34-35 0.3593359483616183 1103 36-37 0.35641485471018086 1103 38-39 0.35514010980502064 1103 40-41 0.36542198219855493 1103 42-43 0.3427473282269844 1103 44-45 0.29402246944417954 1103 46-47 0.30611258280880094 1103 48-49 0.19977219905771193 1103 50-51 0.17224600983579563 1103 52-53 0.2804720556516713 1103 54-55 0.28188412437243215 1103 56-57 0.31571790131150834 1103 58-59 0.31373770936018275 1103 60-61 0.3001698837097706 1103 62-63 0.3256045409865962 1103 64-65 0.2716458882344881 1103 66-67 0.30888398121159355 1103 68-69 0.3003069230813935 1103 70-71 0.24322749140709732 1103 72-73 0.3103804684024425 1103 74-75 0.2835577780301861 1103 76-77 0.21152338448822405 1103 78-79 0.18120618429097135 1103 80-81 0.19817754020800749 1103 82-83 0.1972851823780033 1103 84-85 0.19623228598653952 1103 86-87 0.18553841113553915 1103 88-89 0.20486976957030123 1103 90-91 0.25525089320125716 1103 92-93 0.26177494746775665 1103 94-95 0.2694462816276868 1103 96-97 0.2887365741327308 1103 98-99 0.29335888101810426 1103 100 0.31883821797297074 1104 1 0.09146163161197052 1104 2 0.11941499019128798 1104 3 0.13793741818735583 1104 4 0.04416443648695889 1104 5 0.005046662203433527 1104 6 0.09618765806182239 1104 7 0.062413315161457206 1104 8 0.11547573415231938 1104 9 0.1933002210433159 1104 10-11 0.1399352825265936 1104 12-13 0.15038288385157017 1104 14-15 0.20617552278662288 1104 16-17 0.30536286548809244 1104 18-19 0.26092368675528377 1104 20-21 0.22166318853307132 1104 22-23 0.24354507442664186 1104 24-25 0.22391080904803573 1104 26-27 0.21713929404264576 1104 28-29 0.1677750059922758 1104 30-31 0.18756226401026055 1104 32-33 0.15087767847739286 1104 34-35 0.1606848845853932 1104 36-37 0.1486622696611235 1104 38-39 0.15332302393878194 1104 40-41 0.16255509941441915 1104 42-43 0.21116073470223995 1104 44-45 0.21299563276031108 1104 46-47 0.20835817280771352 1104 48-49 0.12457311746707234 1104 50-51 0.15774460830858317 1104 52-53 0.20783873975877043 1104 54-55 0.19769859237644027 1104 56-57 0.1857164539357612 1104 58-59 0.18563421210480868 1104 60-61 0.19645417347979333 1104 62-63 0.20927454650639987 1104 64-65 0.2517470786785623 1104 66-67 0.27747457884293425 1104 68-69 0.252709746632803 1104 70-71 0.23825908195617984 1104 72-73 0.21973631427221108 1104 74-75 0.30266205920585065 1104 76-77 0.24946494646736994 1104 78-79 0.26241659496810854 1104 80-81 0.238444477650809 1104 82-83 0.31446831946156095 1104 84-85 0.29277386702429453 1104 86-87 0.2789175210983359 1104 88-89 0.250411222951314 1104 90-91 0.25729964908740044 1104 92-93 0.2971941650218213 1104 94-95 0.34420476765466645 1104 96-97 0.3127893970548463 1104 98-99 0.3054233422796777 1104 100 0.24543289342816976 1105 1 -0.007924186771440134 1105 2 0.10170481869148773 1105 3 0.12384838178214608 1105 4 -0.06798041616380601 1105 5 -0.08172777914494134 1105 6 -0.05706080104889821 1105 7 -0.41795080938515383 1105 8 -0.3232894528016672 1105 9 -0.2972020671908453 1105 10-11 -0.31993229701808445 1105 12-13 -0.19273737617010056 1105 14-15 -0.16195089716866562 1105 16-17 -0.08078163206061362 1105 18-19 -0.07960903879793335 1105 20-21 -0.09945007452871124 1105 22-23 -0.07984709231190124 1105 24-25 -0.14051303786674652 1105 26-27 -0.1219095216195214 1105 28-29 -0.13518312095308715 1105 30-31 -0.07195854750664665 1105 32-33 -0.0928387362963079 1105 34-35 -0.12465809689494733 1105 36-37 -0.16670866704736653 1105 38-39 -0.1903866873777389 1105 40-41 -0.11860909180441581 1105 42-43 -0.20877063639027682 1105 44-45 -0.14582675098905895 1105 46-47 -0.0793493173092159 1105 48-49 -0.11346592201810068 1105 50-51 -0.0618121844676196 1105 52-53 -0.04675358134045382 1105 54-55 -0.046871879075894185 1105 56-57 4.5093641240612214E-5 1105 58-59 0.004809994869312106 1105 60-61 0.004574053034971826 1105 62-63 -0.02808074034721386 1105 64-65 0.06950689199634041 1105 66-67 0.09738620191296832 1105 68-69 0.03263579119447968 1105 70-71 0.0015300248246035153 1105 72-73 -0.0017065052381184387 1105 74-75 -0.062206933500064565 1105 76-77 -0.023977767961724794 1105 78-79 -0.042898068935038225 1105 80-81 -0.04525171804666428 1105 82-83 0.04249674211997245 1105 84-85 0.047387657303922026 1105 86-87 0.04794084550580635 1105 88-89 0.06029628583699065 1105 90-91 0.03388909313527222 1105 92-93 0.03555270576978131 1105 94-95 0.0493901635391083 1105 96-97 0.08487085674170558 1105 98-99 0.039602277480799586 1105 100 -0.04433581918193852 1106 1 0.09805766951600248 1106 2 0.10603872263962444 1106 3 0.11519384927497356 1106 4 0.05515443421745658 1106 5 0.06758715971848517 1106 6 0.09964733816023852 1106 7 0.0947838629016573 1106 8 0.1154648799959972 1106 9 0.16046458915051431 1106 10-11 0.16947215541126326 1106 12-13 0.1631763161002482 1106 14-15 0.1365452054834435 1106 16-17 0.21897776002187186 1106 18-19 0.20422848133215865 1106 20-21 0.1550199021576617 1106 22-23 0.1987720085221767 1106 24-25 0.2076113198512104 1106 26-27 0.22076384219727885 1106 28-29 0.19468944892737028 1106 30-31 0.17202659495020356 1106 32-33 0.07965673390265948 1106 34-35 0.08588889338712846 1106 36-37 0.1623134269444293 1106 38-39 0.1628609493477029 1106 40-41 0.15858001134773758 1106 42-43 0.2080403292172761 1106 44-45 0.22525203095653978 1106 46-47 0.21048723201548825 1106 48-49 0.25481345480110207 1106 50-51 0.23632335521268288 1106 52-53 0.2367112067111492 1106 54-55 0.2821637456009398 1106 56-57 0.23446863710128696 1106 58-59 0.25413882183210035 1106 60-61 0.23807657382712932 1106 62-63 0.22272284618636462 1106 64-65 0.1837702934165364 1106 66-67 0.25935964977320225 1106 68-69 0.23466125815629368 1106 70-71 0.20407646570981086 1106 72-73 0.30613087601098243 1106 74-75 0.31047425598841727 1106 76-77 0.2761877148692804 1106 78-79 0.2494254032804193 1106 80-81 0.22735226994830526 1106 82-83 0.24750738455690424 1106 84-85 0.21908779553816515 1106 86-87 0.22283524659343357 1106 88-89 0.2671470163408074 1106 90-91 0.24736390240367712 1106 92-93 0.2710016879388917 1106 94-95 0.2638810506652831 1106 96-97 0.18821303806513967 1106 98-99 0.24498871554753876 1106 100 0.23414457085580054 1107 1 -0.06059853938569404 1107 2 -0.03179649935139395 1107 3 -0.042926092552960426 1107 4 -0.10749726821781991 1107 5 -0.13886168731490045 1107 6 -0.09363713967286458 1107 7 -0.04313149531639482 1107 8 -0.06247294067234321 1107 9 0.017620814990962685 1107 10-11 0.0027447852361319747 1107 12-13 0.016108071481653496 1107 14-15 -0.025042508136564834 1107 16-17 -0.03804950346194147 1107 18-19 0.009267561225463794 1107 20-21 -0.014572622978143102 1107 22-23 -0.03643514780241475 1107 24-25 -0.039690330073952396 1107 26-27 -0.023866954633312787 1107 28-29 -0.03942572787138232 1107 30-31 -0.006675216401980322 1107 32-33 0.017895819690352255 1107 34-35 0.07453829944475387 1107 36-37 0.0033007067805854717 1107 38-39 0.07868874290038264 1107 40-41 0.08625980096090302 1107 42-43 0.1143059339434771 1107 44-45 0.13515939043975322 1107 46-47 0.06488855667111437 1107 48-49 0.029076805382580062 1107 50-51 0.03564294927218015 1107 52-53 0.004129518271994925 1107 54-55 -0.02301999727281867 1107 56-57 0.014404531158000111 1107 58-59 -0.054996776411805115 1107 60-61 -0.07079396719950637 1107 62-63 -0.04134166932519889 1107 64-65 0.08913628354102343 1107 66-67 0.11032856132270297 1107 68-69 0.022080318668031396 1107 70-71 0.021782120714462394 1107 72-73 0.020887039322381895 1107 74-75 0.035095986411363356 1107 76-77 0.054732011061283004 1107 78-79 0.04838533379965071 1107 80-81 0.042290186780114425 1107 82-83 0.04816103791052484 1107 84-85 -0.017300667518881596 1107 86-87 0.031243390196337373 1107 88-89 0.10708496735394846 1107 90-91 0.014612370738319669 1107 92-93 0.01800121289437584 1107 94-95 0.07691069985394705 1107 96-97 0.09782932454174542 1107 98-99 0.11648013504647992 1107 100 0.11854870901460757 1108 1 -0.07554454422144374 1108 2 -0.07930134181633974 1108 3 -0.05165096736572394 1108 4 -0.02068019765149387 1108 5 -0.17251663200551093 1108 6 -0.1467117502221953 1108 7 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-0.30863136684723713 2214 88-89 -0.30505312378186034 2214 90-91 -0.3238786240445819 2214 92-93 -0.36164611245173006 2214 94-95 -0.4210744808947453 2214 96-97 -0.3908884342162189 2214 98-99 -0.3040071027686828 2214 100 -0.3581785831728368 2215 1 -0.13875964257250928 2215 2 -0.17953997620298168 2215 3 -0.16448633292702652 2215 4 -0.09705409198112136 2215 5 -0.14298357182772747 2215 6 -0.20732844613596058 2215 7 -0.2292146232075183 2215 8 -0.2200361246428031 2215 9 -0.21191256085246124 2215 10-11 -0.22827607830148366 2215 12-13 -0.22657779648088194 2215 14-15 -0.3032709802446476 2215 16-17 -0.31821031875674777 2215 18-19 -0.3505802122806685 2215 20-21 -0.3250082422210312 2215 22-23 -0.3158032666474142 2215 24-25 -0.31644172847749985 2215 26-27 -0.3376845855315054 2215 28-29 -0.3700452348772174 2215 30-31 -0.37694883233309184 2215 32-33 -0.4396707008675875 2215 34-35 -0.4285583894477867 2215 36-37 -0.42507471562830546 2215 38-39 -0.4187181736741792 2215 40-41 -0.43021451406406186 2215 42-43 -0.4307712943539954 2215 44-45 -0.4599139291859302 2215 46-47 -0.4259289422023542 2215 48-49 -0.41619750766921015 2215 50-51 -0.35665872126192966 2215 52-53 -0.4075067517630373 2215 54-55 -0.49467099351137733 2215 56-57 -0.4588802624781678 2215 58-59 -0.35085366654373473 2215 60-61 -0.3120551739712596 2215 62-63 -0.37694228350394354 2215 64-65 -0.35676924060775406 2215 66-67 -0.4163331705744753 2215 68-69 -0.43622038816096165 2215 70-71 -0.4425084800226955 2215 72-73 -0.42985831800798735 2215 74-75 -0.46392030264426865 2215 76-77 -0.4179231815355209 2215 78-79 -0.4778031439292434 2215 80-81 -0.48837658055511923 2215 82-83 -0.3984355092626899 2215 84-85 -0.3730699252980827 2215 86-87 -0.40307259669600626 2215 88-89 -0.49090817462278125 2215 90-91 -0.4370588382320122 2215 92-93 -0.4625809784570656 2215 94-95 -0.45707775336096645 2215 96-97 -0.41994578000752014 2215 98-99 -0.49750290015328247 2215 100 -0.5579205593361607 2216 1 -0.32023950048121463 2216 2 -0.30829533007461407 2216 3 -0.43283454087070794 2216 4 -0.2514166156937634 2216 5 -0.34297958217497637 2216 6 -0.24482776410420115 2216 7 -0.3774955365223036 2216 8 -0.331752119036409 2216 9 -0.44215535918744564 2216 10-11 -0.39716907892180586 2216 12-13 -0.3741356318419591 2216 14-15 -0.5412438240082338 2216 16-17 -0.6473988668873005 2216 18-19 -0.5315820265162259 2216 20-21 -0.5418092044109315 2216 22-23 -0.5805204866474298 2216 24-25 -0.6108528712669212 2216 26-27 -0.6621062754216425 2216 28-29 -0.680758832123388 2216 30-31 -0.6751526620765773 2216 32-33 -0.5991864949831083 2216 34-35 -0.6254697829887448 2216 36-37 -0.6700411037360041 2216 38-39 -0.68580628521741 2216 40-41 -0.7155825060867471 2216 42-43 -0.6255228688685932 2216 44-45 -0.6996727950705832 2216 46-47 -0.663237993506435 2216 48-49 -0.7403713046490807 2216 50-51 -0.6592660525533773 2216 52-53 -0.7362243717226278 2216 54-55 -0.8015039698681505 2216 56-57 -0.833066127846898 2216 58-59 -0.8643313909203769 2216 60-61 -0.7657986303503748 2216 62-63 -0.8063028092829967 2216 64-65 -0.8008660646328707 2216 66-67 -0.7102627896198115 2216 68-69 -0.6903530656169252 2216 70-71 -0.6779449735131635 2216 72-73 -0.753976402780772 2216 74-75 -0.7671632129629629 2216 76-77 -0.6856006058057602 2216 78-79 -0.7119827414416378 2216 80-81 -0.7370709635839034 2216 82-83 -0.7335674592595396 2216 84-85 -0.7142582241064162 2216 86-87 -0.682994246627473 2216 88-89 -0.6171777324660965 2216 90-91 -0.5710956036900257 2216 92-93 -0.6708825820364694 2216 94-95 -0.6597650392822274 2216 96-97 -0.7199763984173693 2216 98-99 -0.7791902821493615 2216 100 -0.8189811258693389 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36.0 3 192.0 4 118.0 5 170.0 6 273.0 7 525.0 8 879.0 9 1405.0 10 2042.0 11 3182.0 12 5306.0 13 7773.0 14 7655.0 15 9040.0 16 10953.0 17 16272.0 18 19798.0 19 10565.0 20 6754.0 21 5885.0 22 5850.0 23 6144.0 24 6906.0 25 8535.0 26 9999.0 27 12660.0 28 15289.0 29 18545.0 30 23453.0 31 28935.0 32 36473.0 33 47501.0 34 66187.0 35 106902.0 36 175262.0 37 402072.0 38 768986.0 39 455843.0 40 6228.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.89357650130367 21.557856848534797 14.522600493769023 24.02596615639251 2 14.154115415393365 22.05321317947384 40.49220265100777 23.300468754125024 3 15.17043728300959 27.700332296655482 32.08346280986728 25.045767610467646 4 10.764345434762474 18.84133742706206 39.67780496735375 30.71651217082171 5 11.211624028983035 37.298953125885866 35.06640935898274 16.42301348614836 6 27.710202532423494 39.73434525249579 18.72220681011325 13.833245404967471 7 23.822585803730902 33.512998611179036 24.467312070970525 18.197103514119537 8 23.596669772651435 34.00200727691982 23.077019622235504 19.32430332819324 9 24.122119300110402 15.64386285252314 23.71811045908994 36.51590738827652 10-11 22.04202557525276 26.869076466517473 30.43450317732288 20.65439478090689 12-13 24.11629828360079 25.033378877197325 30.155354058460315 20.69496878074157 14-15 21.840150991542 26.533145387352942 28.623777532434314 23.002926088670744 16-17 19.722556071103824 29.905374940545563 28.536851795188507 21.8352171931621 18-19 19.198599666838774 27.39218893158596 32.701973043283694 20.707238358291573 20-21 21.25112471127542 27.025378333613926 31.730815422707504 19.992681532403154 22-23 20.501153493513236 27.669692085071794 31.87837995455272 19.950774466862246 24-25 20.420286229676204 26.96008209151594 32.140875585131425 20.478756093676427 26-27 20.12737483456549 26.75540188575865 32.30441507810586 20.812808201569993 28-29 20.168590215500657 26.889109324829814 32.18000470013283 20.762295759536702 30-31 20.875192162538344 27.829896336100933 31.394662901896698 19.90024859946402 32-33 19.70341756880312 27.187877017296135 32.55823716856291 20.550468245337836 34-35 19.801372542909277 26.407730768298553 32.36220469032953 21.42869199846264 36-37 21.148225067642386 27.761522252901056 30.93718168367708 20.15307099577948 38-39 20.704762209784448 27.414971668764316 31.470718330560516 20.409547790890727 40-41 20.11036947654892 27.189536345191023 31.661337400541523 21.038756777718532 42-43 19.97340929687845 27.435663395629327 31.535755926428926 21.055171381063296 44-45 20.89867595842943 27.403717772660247 31.015218276646916 20.682387992263404 46-47 20.616284044473865 28.6183164825491 30.076859138754365 20.68854033422267 48-49 20.47524697625583 27.83223099648308 30.56004944285896 21.13247258440213 50-51 20.637798364778266 27.33081202567241 30.41896308427323 21.612426525276085 52-53 20.650131805990927 27.888100587165287 29.994854134847632 21.46691347199615 54-55 21.057368359664682 28.63771974679145 29.47084191431133 20.834069979232535 56-57 20.47232286431916 28.00993083772671 29.79158267803264 21.726163619921486 58-59 19.97746886550804 27.247092235046562 30.639594681942484 22.135844217502918 60-61 21.317786346067038 28.119607701419596 29.341028924684508 21.221577027828857 62-63 20.864600559250373 27.97448533774663 29.744766819599988 21.416147283403006 64-65 20.606593199235 27.354319865073595 30.480227370203234 21.558859565488167 66-67 20.688906739191214 27.85429075199496 29.59020139584363 21.866601112970198 68-69 21.514973873380843 27.93517369814304 29.387323369144774 21.162529059331348 70-71 21.744990145186463 27.413001082860934 29.601358293667385 21.240650478285218 72-73 21.066403414886853 27.707891797286322 30.235905217241143 20.98979957058568 74-75 20.986186020865954 27.382757758082683 30.01993457857366 21.611121642477706 76-77 21.26300291029753 27.841456840973635 29.360810027402113 21.534730221326733 78-79 21.491992575872366 28.048110978460116 29.46223980226563 20.99765664340189 80-81 21.16373962001699 27.443361549950513 29.73551628013262 21.65738254989987 82-83 20.680398619908836 27.635018514895876 29.98848337121717 21.696099493978114 84-85 21.066266824001207 27.667609216837967 29.521909152070556 21.744214807090266 86-87 21.306961973956838 27.89647654546733 29.383695705096645 21.41286577547918 88-89 20.496521291092122 27.689791480879816 29.74486005638038 22.068827171647683 90-91 20.434732178369728 27.460053865985962 29.569552517605125 22.535661438039185 92-93 20.779354835358955 28.064880450408165 29.392196973287255 21.763567740945625 94-95 20.609621720064887 27.557968530627154 29.704587278257605 22.127822471050358 96-97 20.377975595378757 27.733899260174866 29.78882480591168 22.099300338534704 98-99 20.20143745400162 27.53146608146525 29.810605167139247 22.45649129739388 100 20.87191967491174 27.733345567279272 29.191767535406672 22.202967222402318 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1077.0 1 719.0 2 1887.0 3 2891.5 4 1516.5 5 729.5 6 1407.0 7 3340.5 8 5358.0 9 5659.0 10 3819.5 11 2073.0 12 1830.5 13 1988.0 14 2148.5 15 2332.5 16 2561.0 17 2972.0 18 3417.5 19 3879.5 20 4792.5 21 5831.0 22 7427.0 23 10098.5 24 13298.0 25 17011.5 26 22084.5 27 28579.0 28 35910.0 29 43411.0 30 50019.5 31 56062.5 32 61004.0 33 65289.0 34 69718.0 35 73078.0 36 75096.0 37 76988.5 38 78569.0 39 79387.5 40 79518.0 41 78211.5 42 75762.5 43 73387.5 44 70871.5 45 68356.5 46 66922.0 47 65754.5 48 65559.0 49 66532.5 50 66346.0 51 64023.0 52 60091.0 53 55969.5 54 52135.5 55 48879.0 56 46435.5 57 43798.0 58 41092.0 59 38060.0 60 33787.0 61 28671.5 62 23777.5 63 19238.5 64 14996.0 65 11675.5 66 8652.5 67 5808.0 68 3825.5 69 2430.0 70 1581.5 71 1096.0 72 777.0 73 569.0 74 444.5 75 306.5 76 177.0 77 127.0 78 95.0 79 51.0 80 25.5 81 14.0 82 8.5 83 5.5 84 3.0 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 2.0 95 2.5 96 3.5 97 5.5 98 4.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008699065564554206 2 0.0 3 3.0295253209890275E-4 4 0.0028996885215180693 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.5147626604945137E-4 24-25 4.3278933156986105E-5 26-27 4.7606826472684717E-4 28-29 8.655786631397222E-4 30-31 0.0021206677246923193 32-33 0.0045659274480620345 34-35 0.005929213842507097 36-37 0.00417641704964916 38-39 0.0029862463878320413 40-41 0.0035055935857158747 42-43 4.7606826472684717E-4 44-45 6.924629305117777E-4 46-47 0.002921327988096562 48-49 0.0020341098583783472 50-51 8.439391965612291E-4 52-53 0.0 54-55 3.245919986773958E-4 56-57 5.409866644623263E-4 58-59 5.409866644623263E-4 60-61 2.5967359894191666E-4 62-63 0.0 64-65 0.0 66-67 8.22299729982736E-4 68-69 8.006602634042429E-4 70-71 0.0020341098583783472 72-73 1.7311573262794442E-4 74-75 0.0015147626604945137 76-77 1.9475519920643747E-4 78-79 8.006602634042429E-4 80-81 6.924629305117777E-4 82-83 9.088575962967083E-4 84-85 7.573813302472568E-4 86-87 2.3803413236342358E-4 88-89 0.0016229599933869792 90-91 0.0019042730589073887 92-93 0.002878049054939576 94-95 0.001255089061552597 96-97 0.0020341098583783472 98-99 0.0021423071912708125 100 0.003548872518872861 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2310593.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.00789529311112 #Duplication Level Percentage of deduplicated Percentage of total 1 83.34416379932125 59.18093656354107 2 11.314618961490025 16.068545569978667 3 2.630612132196022 5.603826925192887 4 0.9868625094955906 2.8030011897183913 5 0.4927969732324033 1.749623793802429 6 0.2807175482481295 1.195989736376523 7 0.18819743724227153 0.9354452742691748 8 0.12857298229812902 0.730375749163886 9 0.10335441244265096 0.6605081367127903 >10 0.48762551733785714 6.22927402340959 >50 0.028845676092842637 1.4002073981811893 >100 0.012473245861747778 1.40698548608353 >500 6.708869553807853E-4 0.3128535158188193 >1k 3.0494861608118166E-4 0.517005159445662 >5k 1.2197944643247266E-4 0.6946526015671318 >10k+ 6.098972321623633E-5 0.5107688767383262 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11794 0.5104317376534941 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9257 0.40063308423422034 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6783 0.29356100360383675 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4408 0.19077353735599475 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2426 0.10499469183884831 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.1639466578493053E-5 0.0 12-13 0.0 0.0 0.0 6.491839973547916E-5 0.0 14-15 0.0 0.0 0.0 1.2983679947095833E-4 0.0 16-17 0.0 0.0 0.0 1.7311573262794442E-4 0.0 18-19 0.0 0.0 0.0 1.7311573262794442E-4 0.0 20-21 0.0 0.0 0.0 2.3803413236342358E-4 0.0 22-23 0.0 0.0 0.0 3.89510398412875E-4 0.0 24-25 0.0 0.0 0.0 6.924629305117777E-4 0.0 26-27 0.0 0.0 0.0 0.001146891728660132 0.0 28-29 0.0 0.0 0.0 0.0025318175896836873 0.0 30-31 0.0 0.0 0.0 0.0059508533090855895 0.0 32-33 0.0 0.0 0.0 0.013741061277343088 0.0 34-35 0.0 0.0 0.0 0.026897856957066868 0.0 36-37 0.0 0.0 0.0 0.04607042434561171 0.0 38-39 0.0 0.0 0.0 0.07026334798036694 0.0 40-41 0.0 0.0 0.0 0.0984379334655649 0.0 42-43 0.0 0.0 0.0 0.12856007094282723 0.0 44-45 0.0 0.0 0.0 0.16305338066894515 0.0 46-47 0.0 0.0 0.0 0.20501230636464318 0.0 48-49 0.0 0.0 0.0 0.2502604309802722 0.0 50-51 0.0 0.0 0.0 0.2997066121121288 0.0 52-53 0.0 0.0 0.0 0.35053771910500897 0.0 54-55 0.0 0.0 0.0 0.40740623727328873 0.0 56-57 0.0 0.0 0.0 0.475007930864501 0.0 58-59 0.0 0.0 0.0 0.5493827774947817 0.0 60-61 0.0 0.0 0.0 0.6251209105195072 0.0 62-63 0.0 0.0 0.0 0.7002098595468782 0.0 64-65 0.0 0.0 0.0 0.7903165983797233 0.0 66-67 0.0 0.0 0.0 0.894012922223862 0.0 68-69 0.0 0.0 0.0 1.0085289793572472 0.0 70-71 0.0 0.0 0.0 1.135011661508539 0.0 72-73 0.0 0.0 0.0 1.2870938326221883 0.0 74-75 0.0 0.0 0.0 1.440993718928431 0.0 76-77 0.0 0.0 0.0 1.607682529982563 0.0 78-79 0.0 0.0 0.0 1.7962272022809729 0.0 80-81 0.0 0.0 0.0 2.0042906734331836 0.0 82-83 0.0 0.0 0.0 2.2271988186582403 0.0 84-85 0.0 0.0 0.0 2.451967958009048 0.0 86-87 0.0 0.0 0.0 2.698376563938348 0.0 88 0.0 0.0 0.0 2.9013763999111917 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4500 0.0 59.015965 1 GTATCAA 8905 0.0 45.869015 1 ATCAACG 11965 0.0 34.016666 3 TATCAAC 12165 0.0 33.45741 2 TCAACGC 12220 0.0 33.383747 4 CAACGCA 12435 0.0 32.843628 5 TATAACG 230 0.0 32.6948 2 GTCTCGC 1155 0.0 31.74287 1 TACCTGG 2005 0.0 31.645061 2 AACGCAG 12955 0.0 31.634153 6 TAGATCG 205 1.8189894E-12 29.803457 6 CGCTCTG 1065 0.0 29.566668 5 ATAACGC 255 0.0 29.48943 3 GTACCTG 2180 0.0 29.323511 1 TCGCTCT 1385 0.0 28.504671 4 ACGCAGA 14475 0.0 28.182423 7 CGCAGAG 14460 0.0 28.08165 8 TGGTATC 1795 0.0 26.706827 2 TCTGTCG 830 0.0 26.046951 8 GCAGAGT 15555 0.0 25.953762 9 >>END_MODULE