FastQCFastQC Report
Wed 25 May 2016
SRR2431351_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431351_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3186145
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330891.0385277506202637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45550.047.980621
GTATCAA84350.039.283061
TCAACGC101000.030.9918374
ATCAACG102300.030.5983
TATCAAC114350.028.2778722
CAACGCA114600.027.4369585
TACATGG191150.026.8990822
GTACATG198900.026.749331
AACGCAG119350.026.4237566
ACGCAGA125500.024.6045887
ACATGGG202950.024.3856183
CGCAGAG126200.024.2074178
CATGGGG107700.022.8234044
GCAGAGT138500.021.1074149
CCGGTCG8400.020.1426989
CATGGGT29000.019.123994
ATACGAC1250.006413899418.7998523
CATGGGA66850.018.560814
CCCGGTC9650.018.5076268
TACGACT1550.001033926418.1934054
Produced by FastQC (version 0.11.5)