##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431351_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3186145 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.803976278543505 33.0 31.0 34.0 26.0 34.0 2 30.928851323464563 33.0 31.0 34.0 26.0 34.0 3 31.004385864422368 34.0 31.0 34.0 27.0 34.0 4 34.15270679771322 37.0 35.0 37.0 30.0 37.0 5 34.3572467668609 37.0 35.0 37.0 31.0 37.0 6 34.362851345434684 37.0 35.0 37.0 31.0 37.0 7 34.30367952494315 37.0 35.0 37.0 31.0 37.0 8 34.26372559943129 37.0 35.0 37.0 30.0 37.0 9 35.6779901730775 39.0 37.0 39.0 30.0 39.0 10-11 35.71931597588936 39.0 37.0 39.0 30.0 39.0 12-13 35.65555491040113 39.0 37.0 39.0 30.0 39.0 14-15 36.98250456272392 41.0 38.0 41.0 30.0 41.0 16-17 36.99563877350215 40.5 38.0 41.0 30.0 41.0 18-19 37.01465266018967 40.5 38.0 41.0 30.0 41.0 20-21 37.06380327951176 41.0 38.0 41.0 30.0 41.0 22-23 37.0879120379016 40.0 38.0 41.0 30.0 41.0 24-25 37.11789074257449 40.0 38.0 41.0 30.0 41.0 26-27 37.13107517077848 40.0 38.0 41.0 30.0 41.0 28-29 37.10774133003991 40.0 38.0 41.0 30.0 41.0 30-31 37.033288974607245 40.0 38.0 41.0 30.0 41.0 32-33 36.944670597226434 40.0 38.0 41.0 30.0 41.0 34-35 36.866866385553706 40.0 37.0 41.0 30.0 41.0 36-37 36.758490118936834 40.0 37.0 41.0 29.5 41.0 38-39 36.64480634120544 40.0 37.0 41.0 29.0 41.0 40-41 36.53069320448378 40.0 37.0 41.0 28.5 41.0 42-43 36.3857085914169 40.0 36.5 41.0 28.0 41.0 44-45 36.22833800721562 40.0 36.0 41.0 28.0 41.0 46-47 36.07438032481259 40.0 35.5 41.0 28.0 41.0 48-49 35.91499068623682 40.0 35.0 41.0 27.0 41.0 50-51 35.742814435626755 40.0 35.0 41.0 27.0 41.0 52-53 35.53859130704974 39.0 35.0 41.0 27.0 41.0 54-55 35.33324064033495 39.0 35.0 41.0 27.0 41.0 56-57 35.09686847271546 39.0 34.5 41.0 27.0 41.0 58-59 34.83446217921657 38.0 34.0 40.5 26.5 41.0 60-61 34.61797658298665 38.0 34.0 40.0 26.0 41.0 62-63 34.41809428007828 37.5 34.0 40.0 26.0 41.0 64-65 34.12863460388651 37.0 34.0 40.0 26.0 41.0 66-67 33.79766049567738 36.5 33.5 40.0 25.5 41.0 68-69 33.46567419248025 36.0 33.0 39.0 25.0 41.0 70-71 33.21377354137994 35.5 33.0 39.0 25.0 41.0 72-73 32.96908191560648 35.0 33.0 39.0 24.5 41.0 74-75 32.63090003750614 35.0 33.0 37.5 24.0 40.0 76-77 32.30917048659116 35.0 33.0 37.0 23.0 39.5 78-79 31.99587871863961 35.0 33.0 36.5 22.5 39.0 80-81 31.69468401469487 35.0 32.0 36.0 21.5 39.0 82-83 31.405919065202617 35.0 32.0 36.0 20.0 37.5 84-85 31.149194559569636 35.0 32.0 35.0 20.0 37.0 86-87 30.92458896252368 35.0 32.0 35.0 19.0 37.0 88-89 30.703579247021086 35.0 32.0 35.0 18.0 36.0 90-91 30.52316467078554 35.0 31.0 35.0 16.5 36.0 92-93 30.347476338961346 35.0 31.0 35.0 11.0 36.0 94-95 30.137420299452785 35.0 31.0 35.0 4.5 35.5 96-97 29.96512007457288 35.0 31.0 35.0 2.0 35.0 98-99 29.85573161296802 35.0 31.0 35.0 2.0 35.0 100 29.820289409301836 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4739580464540474 1101 2 0.4745193150351703 1101 3 0.5054066583056063 1101 4 0.5677629114423013 1101 5 0.4289806517301784 1101 6 0.5194264491818004 1101 7 0.48708208608579895 1101 8 0.45333766266904263 1101 9 0.6212807913273224 1101 10-11 0.47924538657491667 1101 12-13 0.5476278352058159 1101 14-15 0.6741118296161446 1101 16-17 0.5987857003409331 1101 18-19 0.6172829794374195 1101 20-21 0.6316823184833282 1101 22-23 0.5596523906027926 1101 24-25 0.5685659849846445 1101 26-27 0.5149250781896271 1101 28-29 0.5652405147057209 1101 30-31 0.6007714598589402 1101 32-33 0.5379962366055082 1101 34-35 0.5886525135819909 1101 36-37 0.5724587070720588 1101 38-39 0.5125316730175982 1101 40-41 0.5126061432631914 1101 42-43 0.5714983009751933 1101 44-45 0.5876003969879591 1101 46-47 0.6005031638238734 1101 48-49 0.5583507367508531 1101 50-51 0.48460340422182924 1101 52-53 0.474893843119375 1101 54-55 0.5133466241938365 1101 56-57 0.4729691160161593 1101 58-59 0.46363882258730627 1101 60-61 0.5567764155591632 1101 62-63 0.544012109184763 1101 64-65 0.5287987383090496 1101 66-67 0.4696455599579821 1101 68-69 0.47004077853318194 1101 70-71 0.4666942673339918 1101 72-73 0.5599375274370644 1101 74-75 0.5184659716106879 1101 76-77 0.4625542421960205 1101 78-79 0.4664498510866153 1101 80-81 0.4375597625049288 1101 82-83 0.40900196379416087 1101 84-85 0.42701198085581993 1101 86-87 0.4554249112596942 1101 88-89 0.398298076872166 1101 90-91 0.38932587409891184 1101 92-93 0.4726535080637433 1101 94-95 0.43955055820088873 1101 96-97 0.40050942366548625 1101 98-99 0.41383782804516045 1101 100 0.49562291465122144 1102 1 0.04471850222249785 1102 2 0.165275095202162 1102 3 0.17844217072696011 1102 4 0.03978662531965682 1102 5 0.1269041772544952 1102 6 0.13355018871254032 1102 7 0.16747583133116706 1102 8 0.1841149401319626 1102 9 0.15075882747805736 1102 10-11 0.1776419089906227 1102 12-13 0.13775161380058876 1102 14-15 0.14566320102522212 1102 16-17 0.19698400302913655 1102 18-19 0.17243816228725706 1102 20-21 0.07221519206932214 1102 22-23 0.05409643903735173 1102 24-25 0.1328362750209422 1102 26-27 0.12730170936441 1102 28-29 0.13713171293632342 1102 30-31 0.13595626693299323 1102 32-33 0.1393367770502607 1102 34-35 0.13827324014870612 1102 36-37 0.18951889345981243 1102 38-39 0.1253920235548165 1102 40-41 0.0783861874256786 1102 42-43 0.05891515400142566 1102 44-45 0.08716666548346552 1102 46-47 0.07156472470153119 1102 48-49 0.07905864737719526 1102 50-51 0.06685064688554121 1102 52-53 0.14493325549976532 1102 54-55 0.10404941622461195 1102 56-57 0.07866116745341145 1102 58-59 0.06814032735774589 1102 60-61 0.10214681501500422 1102 62-63 0.17358833900688353 1102 64-65 0.0954738822720671 1102 66-67 0.1684242654728081 1102 68-69 0.1752687735056 1102 70-71 0.15629997438681897 1102 72-73 0.18541792434367466 1102 74-75 0.17610668365525584 1102 76-77 0.1315939477579775 1102 78-79 0.08075531888236043 1102 80-81 0.12200383308919527 1102 82-83 0.15125228526693135 1102 84-85 0.15782917812874686 1102 86-87 0.13022197968577132 1102 88-89 0.11896411448719846 1102 90-91 0.19166824572022634 1102 92-93 0.15047240039321963 1102 94-95 0.15994415443914534 1102 96-97 0.16792699555034218 1102 98-99 0.11366658061804813 1102 100 0.11974724618318078 1103 1 0.14304063003176637 1103 2 0.19651175025617462 1103 3 0.25184895464300894 1103 4 0.25001577465344127 1103 5 0.18267149335778043 1103 6 0.210695598130755 1103 7 0.3139431398094814 1103 8 0.31532653032891744 1103 9 0.32868059048316667 1103 10-11 0.3387550872722329 1103 12-13 0.2901763799100294 1103 14-15 0.3512848413339711 1103 16-17 0.33366004064752275 1103 18-19 0.32946789908236696 1103 20-21 0.3424097722070769 1103 22-23 0.33939956354398504 1103 24-25 0.30236394879316464 1103 26-27 0.3930141794194739 1103 28-29 0.35130210698016384 1103 30-31 0.25092034495138904 1103 32-33 0.2023309942154441 1103 34-35 0.35963666584374465 1103 36-37 0.3878531356181867 1103 38-39 0.39377101912496215 1103 40-41 0.3494443753221219 1103 42-43 0.2956513851029996 1103 44-45 0.2890845451279489 1103 46-47 0.28540301934360457 1103 48-49 0.29345438125490375 1103 50-51 0.24161867252934144 1103 52-53 0.24763284910665817 1103 54-55 0.23561007785720278 1103 56-57 0.16501228767732812 1103 58-59 0.19573996016455197 1103 60-61 0.149698198469693 1103 62-63 0.2043590594712157 1103 64-65 0.155904326895552 1103 66-67 0.21070244795811277 1103 68-69 0.18622897453412435 1103 70-71 0.18011427624693255 1103 72-73 0.2545403397915962 1103 74-75 0.2752211466352179 1103 76-77 0.23994614941400982 1103 78-79 0.2623885819982519 1103 80-81 0.2702897621880993 1103 82-83 0.2404129987811885 1103 84-85 0.3107698741593232 1103 86-87 0.35529143064196944 1103 88-89 0.30973913221077964 1103 90-91 0.22721773846047455 1103 92-93 0.2719857672883741 1103 94-95 0.1497032988814091 1103 96-97 0.14987360421943663 1103 98-99 0.03404571793703326 1103 100 0.04550818557978076 1104 1 0.13250700622462475 1104 2 0.16869005248664237 1104 3 0.23357758003569984 1104 4 0.21111492392649467 1104 5 0.18339666674985722 1104 6 0.21953413334694716 1104 7 0.16796401706079678 1104 8 0.1921716153616444 1104 9 0.2867942556465195 1104 10-11 0.26984546281873634 1104 12-13 0.2550952684403711 1104 14-15 0.2795325429614053 1104 16-17 0.30152281247680435 1104 18-19 0.21958366682003572 1104 20-21 0.16122752820349717 1104 22-23 0.18107597799321695 1104 24-25 0.18757106160734338 1104 26-27 0.21674805344778747 1104 28-29 0.20717128321573597 1104 30-31 0.23060660646660835 1104 32-33 0.259344953502314 1104 34-35 0.23690096089826795 1104 36-37 0.1714097507104455 1104 38-39 0.1730358172411357 1104 40-41 0.15462296365446093 1104 42-43 0.16347622026700748 1104 44-45 0.10031956630825789 1104 46-47 0.06664289298885251 1104 48-49 0.05461700065380626 1104 50-51 0.08514531192629704 1104 52-53 0.10457300547370352 1104 54-55 0.06184214316508729 1104 56-57 0.0735614276239005 1104 58-59 -0.01949237891903266 1104 60-61 0.03019096199902549 1104 62-63 -0.09597642891893088 1104 64-65 -0.057083133252653795 1104 66-67 -0.017963991830058035 1104 68-69 0.0180741399758233 1104 70-71 0.017488593890114146 1104 72-73 0.033548527351157986 1104 74-75 0.040536745119212014 1104 76-77 0.015594927057961172 1104 78-79 0.035456544530177325 1104 80-81 0.008609299363673273 1104 82-83 0.06958909183526885 1104 84-85 0.0744751986682779 1104 86-87 0.05576135609659261 1104 88-89 0.08307148137678766 1104 90-91 0.06025257153192243 1104 92-93 0.009573125379773728 1104 94-95 0.0014384186228895146 1104 96-97 0.046836581327532656 1104 98-99 -9.658421771518988E-4 1104 100 0.10048572740135597 1105 1 0.16398240526261532 1105 2 0.2771653910313887 1105 3 0.21488071990889068 1105 4 0.294359349776677 1105 5 0.19561732891735062 1105 6 0.24297453386501644 1105 7 0.28743500507580677 1105 8 0.2721212603522787 1105 9 0.2915875881374461 1105 10-11 0.3017981135126462 1105 12-13 0.3580273390186903 1105 14-15 0.3503587507472048 1105 16-17 0.36947791202337754 1105 18-19 0.3206394694466965 1105 20-21 0.3294287834725935 1105 22-23 0.29771116178470436 1105 24-25 0.3602675808610556 1105 26-27 0.37363907481324077 1105 28-29 0.3846894514684749 1105 30-31 0.3887103268795187 1105 32-33 0.44113366163138323 1105 34-35 0.3915234854759717 1105 36-37 0.3899711704625375 1105 38-39 0.44123223989134175 1105 40-41 0.45397765999741324 1105 42-43 0.43342088800222456 1105 44-45 0.415878683701024 1105 46-47 0.39131699416815025 1105 48-49 0.35378303429138214 1105 50-51 0.44603742412475356 1105 52-53 0.4765293717625667 1105 54-55 0.5141816242346593 1105 56-57 0.46308863917738563 1105 58-59 0.4199826608731101 1105 60-61 0.427359623916729 1105 62-63 0.525754871215824 1105 64-65 0.584174634730239 1105 66-67 0.5689134249069454 1105 68-69 0.5052358652041562 1105 70-71 0.46943474669058816 1105 72-73 0.5720711209928027 1105 74-75 0.5232387791289312 1105 76-77 0.44526104537630573 1105 78-79 0.4487597035579327 1105 80-81 0.355699520400524 1105 82-83 0.43851571391675037 1105 84-85 0.38297043450740986 1105 86-87 0.38207092496541506 1105 88-89 0.4267684612196341 1105 90-91 0.4677590554992115 1105 92-93 0.4124948759468623 1105 94-95 0.4146547713061466 1105 96-97 0.40322238634753305 1105 98-99 0.476173427254011 1105 100 0.45456203899806624 1106 1 0.08697574993471235 1106 2 0.11023584532424735 1106 3 0.06997633037924444 1106 4 0.044780851757614926 1106 5 0.05333821737053768 1106 6 0.009639003754031705 1106 7 -0.03679661365212894 1106 8 -0.029970654853435974 1106 9 -0.015610949435419741 1106 10-11 -0.07338608992706952 1106 12-13 0.014180259633064907 1106 14-15 0.015193651551797416 1106 16-17 0.07987124861993067 1106 18-19 0.0363220137893947 1106 20-21 0.04136923593097208 1106 22-23 0.08978695179462193 1106 24-25 0.08589135405875936 1106 26-27 0.1417565745907936 1106 28-29 0.1319512498790445 1106 30-31 0.15634992532430658 1106 32-33 0.17661077743825615 1106 34-35 0.2142491381812306 1106 36-37 0.23473292538417212 1106 38-39 0.17339527210216943 1106 40-41 0.21959467117740417 1106 42-43 0.20944746861818686 1106 44-45 0.1504108520427394 1106 46-47 0.12697846670469914 1106 48-49 0.1141232825993228 1106 50-51 0.05573953426215894 1106 52-53 0.05352317344313917 1106 54-55 0.06500249312577466 1106 56-57 0.059637357657393864 1106 58-59 0.08180279092931642 1106 60-61 0.10216865982187073 1106 62-63 0.1585731987183081 1106 64-65 0.17657382795720622 1106 66-67 0.14862338848661238 1106 68-69 0.14755862464878788 1106 70-71 0.22514845408807105 1106 72-73 0.17582648892346242 1106 74-75 0.17634833757798418 1106 76-77 0.21455036176529063 1106 78-79 0.21417346360086498 1106 80-81 0.19378262463538576 1106 82-83 0.19953452579735398 1106 84-85 0.17146761921799936 1106 86-87 0.14978741819792418 1106 88-89 0.20879812662011688 1106 90-91 0.18019017209918786 1106 92-93 0.21362083728499925 1106 94-95 0.1506588185245441 1106 96-97 0.10295253242446378 1106 98-99 0.13825128883585336 1106 100 0.22757312215766845 1107 1 -0.11668494974699328 1107 2 -0.036640701996653036 1107 3 -0.14972319140066404 1107 4 -0.12734465690596863 1107 5 -0.12270904783043335 1107 6 -0.12039432183574661 1107 7 -0.1730621459992605 1107 8 -0.10031787543292836 1107 9 -0.13217073280998903 1107 10-11 -0.12556507157485441 1107 12-13 -0.14925216895791493 1107 14-15 -0.09694510809251256 1107 16-17 -0.12990946726056052 1107 18-19 -0.04260923011862161 1107 20-21 -0.028788237236000214 1107 22-23 0.012891757266629611 1107 24-25 -0.011033605641387112 1107 26-27 -0.02100697668011975 1107 28-29 0.01153131469441604 1107 30-31 0.02097983497542799 1107 32-33 0.0640304734420738 1107 34-35 0.06257011114368538 1107 36-37 0.036492956471612104 1107 38-39 0.03071722746492611 1107 40-41 0.017020171814380092 1107 42-43 0.015857676038294244 1107 44-45 0.03079776288814884 1107 46-47 -0.02661424942083812 1107 48-49 -0.03578604907127669 1107 50-51 -0.015402563197852714 1107 52-53 -0.0011770731577342985 1107 54-55 0.0037348833629096134 1107 56-57 0.02502397230678355 1107 58-59 -0.004174844957425705 1107 60-61 -0.09237567243201283 1107 62-63 0.027345263046825607 1107 64-65 0.09757719852633073 1107 66-67 0.11060310199127343 1107 68-69 0.10694182121638818 1107 70-71 0.06754680072798891 1107 72-73 0.06503379358219519 1107 74-75 0.08146561401418495 1107 76-77 0.10792917786679368 1107 78-79 0.033611544380093505 1107 80-81 0.09968616501680927 1107 82-83 0.025625310583350824 1107 84-85 0.06214069989310289 1107 86-87 0.07340768560732513 1107 88-89 0.10882918171075673 1107 90-91 0.06302855481638048 1107 92-93 0.09867363397180995 1107 94-95 0.1561573487655501 1107 96-97 0.14275296707442564 1107 98-99 0.14011722263472848 1107 100 0.1861211672740808 1108 1 -0.16379350769603818 1108 2 -0.13022951940260086 1108 3 -0.10606655812534527 1108 4 -0.20314779084446144 1108 5 -0.17635131057630815 1108 6 -0.17305013196809682 1108 7 -0.14673276211347996 1108 8 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2214 96-97 -0.2564212070997449 2214 98-99 -0.1614656101981069 2214 100 -0.15994990379698848 2215 1 -0.2597258349873677 2215 2 -0.2791465618938176 2215 3 -0.2828350390905179 2215 4 -0.25282475885666145 2215 5 -0.13027039068589374 2215 6 -0.22861584258923529 2215 7 -0.13714716635026747 2215 8 -0.2196525553814226 2215 9 -0.257328115256378 2215 10-11 -0.2743799990342808 2215 12-13 -0.30015870208951867 2215 14-15 -0.3679756015015272 2215 16-17 -0.22890866741065707 2215 18-19 -0.24469152798096871 2215 20-21 -0.21485369619148287 2215 22-23 -0.22544228375283382 2215 24-25 -0.3161053196645156 2215 26-27 -0.21146094910340452 2215 28-29 -0.2299609814014616 2215 30-31 -0.2903040649273976 2215 32-33 -0.3649102868286107 2215 34-35 -0.34632031015082987 2215 36-37 -0.3829810663272113 2215 38-39 -0.3509636130394469 2215 40-41 -0.3959249069874815 2215 42-43 -0.27440209414474737 2215 44-45 -0.23020843878301633 2215 46-47 -0.2880986238208152 2215 48-49 -0.25931308359574956 2215 50-51 -0.29639223024889816 2215 52-53 -0.24883011531204602 2215 54-55 -0.23746696883638663 2215 56-57 -0.21233422988517958 2215 58-59 -0.24534158714718757 2215 60-61 -0.22862523949999058 2215 62-63 -0.3483308880768945 2215 64-65 -0.2980151786517311 2215 66-67 -0.27374776330589157 2215 68-69 -0.3195305408058502 2215 70-71 -0.39556556264013665 2215 72-73 -0.30782629705283426 2215 74-75 -0.2893207916827052 2215 76-77 -0.23933736159924024 2215 78-79 -0.25060528114108394 2215 80-81 -0.23506980030181523 2215 82-83 -0.2384297565407678 2215 84-85 -0.28099569554508363 2215 86-87 -0.33598735064193264 2215 88-89 -0.33073118611048 2215 90-91 -0.2850909567302402 2215 92-93 -0.2741222528905709 2215 94-95 -0.22644501977032405 2215 96-97 -0.22785276836506796 2215 98-99 -0.1981508042522222 2215 100 -0.2870724135921314 2216 1 -0.35459826714028253 2216 2 -0.4009378522741258 2216 3 -0.5524911737188951 2216 4 -0.5403357576342458 2216 5 -0.4055469102888125 2216 6 -0.5131988621455577 2216 7 -0.417677808745637 2216 8 -0.5859655751354467 2216 9 -0.5554680990448162 2216 10-11 -0.5706741652081888 2216 12-13 -0.47405534528955684 2216 14-15 -0.5373507104622774 2216 16-17 -0.5614387829430783 2216 18-19 -0.6723792671971651 2216 20-21 -0.6503871551982314 2216 22-23 -0.6073509731658433 2216 24-25 -0.7519107793716913 2216 26-27 -0.8299438123211047 2216 28-29 -0.6517012608185553 2216 30-31 -0.7259255911815785 2216 32-33 -0.7176563609678794 2216 34-35 -0.7619468161841993 2216 36-37 -0.7059477977049156 2216 38-39 -0.6840383397678025 2216 40-41 -0.726657571283603 2216 42-43 -0.7775914828887309 2216 44-45 -0.77404583686058 2216 46-47 -0.6713567255582547 2216 48-49 -0.7357583685446514 2216 50-51 -0.6784783058414092 2216 52-53 -0.7215928156746685 2216 54-55 -0.799634068864087 2216 56-57 -0.6359635260840903 2216 58-59 -0.6742926612980114 2216 60-61 -0.6873643540159122 2216 62-63 -0.7520920977152059 2216 64-65 -0.7414124587345938 2216 66-67 -0.8291815702424543 2216 68-69 -0.8047359493159192 2216 70-71 -0.8972071309971383 2216 72-73 -0.8973821184647832 2216 74-75 -0.8308705274681287 2216 76-77 -0.8681672277447277 2216 78-79 -0.8407737861366513 2216 80-81 -0.7888694848002622 2216 82-83 -0.7802649085183901 2216 84-85 -0.7180291220722239 2216 86-87 -0.8047956582847036 2216 88-89 -0.8397271398954622 2216 90-91 -0.8608451256103571 2216 92-93 -0.92754223403956 2216 94-95 -0.9259890984546857 2216 96-97 -1.0218891522057838 2216 98-99 -1.0502214821980047 2216 100 -1.0444310996801534 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 65385.0 3 14762.0 4 10844.0 5 10035.0 6 6631.0 7 6283.0 8 6775.0 9 7321.0 10 7090.0 11 7604.0 12 8741.0 13 9785.0 14 10155.0 15 10231.0 16 10230.0 17 9826.0 18 9875.0 19 9050.0 20 8632.0 21 9206.0 22 11493.0 23 19681.0 24 33919.0 25 42015.0 26 39777.0 27 28424.0 28 29050.0 29 33486.0 30 38967.0 31 46519.0 32 61616.0 33 83692.0 34 121038.0 35 203302.0 36 365947.0 37 662187.0 38 810209.0 39 314768.0 40 1594.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.97493010333533 21.196453882011166 13.46905903459116 22.359556980062344 2 15.936541454667916 22.425704056597585 38.68577147981117 22.951983008923328 3 17.98634399878223 29.469311660329332 28.61891094096471 23.925433399923733 4 12.176289639228372 19.535155829477834 38.15666779551326 30.131886735780533 5 12.622809068639373 41.14922578853128 33.18938717478332 13.038577968046026 6 28.123139405143206 40.217755312454386 18.337144103611102 13.321961178791298 7 25.806515397133527 35.29807965425302 22.329460837469732 16.56594411114372 8 24.56843615089709 37.4884382223659 19.876935920995432 18.06618970574158 9 26.01513741527771 19.037771350644743 20.134268842127398 34.81282239195015 10-11 23.530708740499882 29.368123547421725 27.354263537911805 19.746904174166588 12-13 24.108821161623215 27.85389553833865 26.879944258657407 21.15733904138073 14-15 22.2592819849693 28.604410659276336 24.813418723881053 24.32288863187331 16-17 21.63636620430018 31.11275538307265 25.492499556674286 21.758378855952884 18-19 21.63727639514209 30.198688383610918 27.247174877477327 20.916860343769667 20-21 23.798979644680326 28.81654789722376 26.35262990228003 21.03184255581588 22-23 23.16839001363717 29.001677575879313 26.439725750083564 21.39020666039995 24-25 22.259799852172453 29.268630272633544 26.597345695189638 21.87422418000436 26-27 22.153323216614435 29.478586191149493 26.556245870793703 21.81184472144237 28-29 22.070841094802653 29.147920135461508 27.190005476837996 21.591233292897844 30-31 22.504029163770014 29.39029767948414 26.51498911694226 21.590684039803588 32-33 22.039925992068785 29.350045274147913 26.708718529759317 21.901310204023986 34-35 22.51598718827925 28.724289070334212 26.916304813497188 21.843418927889346 36-37 22.585020455754528 29.03508785695566 26.475458587101343 21.904433100188474 38-39 22.269090075938163 29.180498690423697 26.653793220333664 21.89661801330448 40-41 22.30057012471184 28.890461670765138 26.773734403173744 22.03523380134928 42-43 22.084321335030264 29.183135105276126 26.92683477996136 21.805708779732246 44-45 22.13274976499814 29.06705438704139 26.713881508845326 22.086314339115138 46-47 22.317440041178287 28.822322901186233 26.7617449927734 22.098492064862082 48-49 22.102211292957477 28.77960670339862 26.78812483424326 22.33005716940064 50-51 22.079268206563103 28.924185810752494 26.616506781706416 22.380039200977983 52-53 22.161703249538235 29.01146997390263 26.54508818650752 22.281738590051614 54-55 22.128983458066095 29.27751247981495 26.533083083161625 22.06042097895733 56-57 22.211434193986776 29.05158114272891 26.436288995008073 22.30069566827624 58-59 22.44850752241345 28.349164899902547 26.78429575552902 22.418031822154987 60-61 22.50407624260666 28.895561878068953 26.55370361361457 22.046658265709816 62-63 21.99925332953355 29.009104891507587 26.698161367131966 22.293480411826895 64-65 22.45868007555841 28.86662861649872 26.519230373889197 22.155460934053682 66-67 22.153307523668886 28.819215697967294 26.546327929205983 22.48114884915784 68-69 22.46468694927569 29.030223043835107 26.587914234913978 21.917175771975224 70-71 22.50909798518272 28.729624671821274 26.626879818715093 22.13439752428091 72-73 22.326243783631945 28.729263734073623 26.57620729753354 22.368285184760893 74-75 22.270816299948685 28.78925786491199 26.68527326910734 22.25465256603199 76-77 22.408600361879323 28.727631667736404 26.426104273346002 22.43766369703827 78-79 22.448695837760052 28.656715246795105 26.608377835911423 22.286211079533416 80-81 22.164135656098512 29.03402073665825 26.65584899620074 22.1459946110425 82-83 22.209810832318652 29.051864143510265 26.71144705908385 22.026877965087237 84-85 22.21730332783345 28.554417322347792 26.634923843274294 22.593355506544466 86-87 22.537668683813116 28.793778227090378 26.508399057594367 22.160154031502135 88-89 22.12728346448661 28.953756092416565 26.58322009158418 22.33574035151264 90-91 22.109186336218613 28.7758728811591 26.503456622572468 22.611484160049816 92-93 22.188016015974544 29.000031072773375 26.535552117672278 22.2764007935798 94-95 22.257160734050863 28.53845022721541 26.766818154069576 22.437570884664147 96-97 22.397071385067445 28.884040715242666 26.404739743625356 22.31414815606453 98-99 21.931078380969062 28.95978850327082 26.558981737283798 22.550151378476322 100 21.9357434710528 28.79554004623919 26.661512936740944 22.607203545967064 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 29405.0 1 19286.0 2 7058.5 3 3904.5 4 2298.5 5 1574.5 6 1443.0 7 1718.0 8 2208.5 9 2316.5 10 1718.0 11 1171.0 12 1069.5 13 1078.0 14 1143.5 15 1227.5 16 1370.5 17 1706.0 18 2122.0 19 2652.5 20 3603.0 21 4958.0 22 6473.0 23 8494.5 24 11669.5 25 15298.0 26 19440.5 27 24551.0 28 30574.0 29 37306.0 30 42642.5 31 47336.0 32 52590.0 33 57249.0 34 62187.0 35 69101.5 36 75297.5 37 81406.5 38 89174.0 39 93230.0 40 97770.5 41 104781.5 42 109857.5 43 118323.5 44 122120.0 45 118804.5 46 118579.0 47 118800.5 48 117746.5 49 117401.5 50 118793.5 51 113867.5 52 103484.5 53 95089.0 54 89897.5 55 87628.5 56 79087.0 57 67585.5 58 61467.5 59 57370.0 60 50613.0 61 41937.5 62 35349.5 63 29257.5 64 23304.0 65 19025.0 66 15141.0 67 11663.0 68 9007.0 69 6847.0 70 5113.5 71 3919.5 72 3036.5 73 2240.5 74 1735.0 75 1316.5 76 928.5 77 700.0 78 517.5 79 359.5 80 247.0 81 181.0 82 132.0 83 105.0 84 101.0 85 80.5 86 69.0 87 64.5 88 52.5 89 38.5 90 33.5 91 34.5 92 28.5 93 19.0 94 13.5 95 13.0 96 13.0 97 11.5 98 10.0 99 13.0 100 38.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.080165843048574E-4 2 6.27717822007473E-5 3 0.0 4 6.27717822007473E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 1.5692945550186825E-5 64-65 1.5692945550186825E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.0985061885130776E-4 84-85 5.649460398067256E-4 86-87 0.0013652862628662538 88-89 0.0035936845309927825 90-91 0.0027305725257325076 92-93 0.0023853277236283972 94-95 0.002244091213676716 96-97 0.002055775867074474 98-99 0.0028561160901340024 100 0.0032327467833384855 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3186145.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.470982772129055 #Duplication Level Percentage of deduplicated Percentage of total 1 74.14692234473378 35.939741955779816 2 13.921505329636735 13.495780899898502 3 4.904445129836767 7.131698261855106 4 2.3230058355416348 4.503935033363753 5 1.2808748444102969 3.1042631258332487 6 0.7767983151818345 2.2591306651558547 7 0.5222933295181759 1.7721249683951397 8 0.35252098445765134 1.3669630851568633 9 0.25496209956060445 1.112243718181312 >10 1.2954286841865492 11.51673032435279 >50 0.11444286362867655 3.871951056827725 >100 0.09744378515934442 9.136774784827029 >500 0.006952365235148806 2.2334429226689814 >1k 0.002339113536723211 1.5131075041534685 >5k 0.0 0.0 >10k+ 6.497537602008919E-5 1.042111693550373 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33089 1.0385277506202637 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.138589110037365E-5 3 0.0 0.0 0.0 0.0 3.138589110037365E-5 4 0.0 0.0 0.0 0.0 3.138589110037365E-5 5 0.0 0.0 0.0 0.0 3.138589110037365E-5 6 0.0 0.0 0.0 0.0 3.138589110037365E-5 7 0.0 0.0 0.0 0.0 3.138589110037365E-5 8 0.0 0.0 0.0 0.0 3.138589110037365E-5 9 0.0 0.0 0.0 0.0 3.138589110037365E-5 10-11 0.0 0.0 0.0 0.0 3.138589110037365E-5 12-13 0.0 0.0 0.0 0.0 3.138589110037365E-5 14-15 0.0 0.0 0.0 1.5692945550186825E-5 3.138589110037365E-5 16-17 0.0 0.0 0.0 3.138589110037365E-5 3.138589110037365E-5 18-19 0.0 0.0 0.0 6.27717822007473E-5 3.138589110037365E-5 20-21 0.0 0.0 0.0 1.883153466022419E-4 3.138589110037365E-5 22-23 0.0 0.0 0.0 4.550954209554179E-4 3.138589110037365E-5 24-25 0.0 0.0 0.0 9.101908419108358E-4 3.138589110037365E-5 26-27 0.0 0.0 0.0 0.0015536016094684956 3.138589110037365E-5 28-29 0.0 0.0 0.0 0.0034053691843905407 3.138589110037365E-5 30-31 0.0 0.0 0.0 0.008176024631647336 3.138589110037365E-5 32-33 0.0 0.0 0.0 0.01611665508004187 3.138589110037365E-5 34-35 0.0 0.0 0.0 0.025830588375607512 3.138589110037365E-5 36-37 0.0 0.0 0.0 0.042010015237850126 3.138589110037365E-5 38-39 0.0 0.0 0.0 0.06429399791911541 3.138589110037365E-5 40-41 0.0 0.0 0.0 0.09094061946333265 3.138589110037365E-5 42-43 0.0 0.0 0.0 0.11473112491741588 3.138589110037365E-5 44-45 0.0 0.0 0.0 0.14274303272449934 3.138589110037365E-5 46-47 0.0 0.0 0.0 0.177738301301416 3.138589110037365E-5 48-49 0.0 0.0 0.0 0.21527582705746284 3.138589110037365E-5 50-51 3.138589110037365E-5 0.0 0.0 0.25568516184919393 3.138589110037365E-5 52-53 3.138589110037365E-5 0.0 0.0 0.29509014812571305 3.138589110037365E-5 54-55 3.138589110037365E-5 0.0 0.0 0.3363939808138048 3.138589110037365E-5 56-57 3.138589110037365E-5 0.0 0.0 0.38741174679746215 3.138589110037365E-5 58-59 3.138589110037365E-5 0.0 0.0 0.4475000353091275 3.138589110037365E-5 60-61 3.138589110037365E-5 0.0 0.0 0.5142107468429717 3.138589110037365E-5 62-63 3.138589110037365E-5 0.0 0.0 0.5821768940208307 3.138589110037365E-5 64-65 3.138589110037365E-5 0.0 0.0 0.6548666178092961 3.138589110037365E-5 66-67 3.138589110037365E-5 0.0 0.0 0.7379921503886357 3.138589110037365E-5 68-69 3.138589110037365E-5 0.0 0.0 0.8375168110679206 3.138589110037365E-5 70-71 3.138589110037365E-5 0.0 0.0 0.9513534380889759 3.138589110037365E-5 72-73 3.138589110037365E-5 0.0 0.0 1.0732091602861766 3.138589110037365E-5 74-75 9.415767330112095E-5 0.0 0.0 1.1967754135483477 3.138589110037365E-5 76-77 9.415767330112095E-5 0.0 0.0 1.3239510442870617 3.138589110037365E-5 78-79 9.415767330112095E-5 0.0 0.0 1.4751211887720115 3.138589110037365E-5 80-81 9.415767330112095E-5 0.0 0.0 1.6535499796776354 3.138589110037365E-5 82-83 9.415767330112095E-5 0.0 0.0 1.8444546622956581 3.138589110037365E-5 84-85 9.415767330112095E-5 0.0 0.0 2.0369914112509004 3.138589110037365E-5 86-87 9.415767330112095E-5 0.0 0.0 2.240811388056727 3.138589110037365E-5 88 9.415767330112095E-5 0.0 0.0 2.4060737976457442 3.138589110037365E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4555 0.0 47.98062 1 GTATCAA 8435 0.0 39.28306 1 TCAACGC 10100 0.0 30.991837 4 ATCAACG 10230 0.0 30.598 3 TATCAAC 11435 0.0 28.277872 2 CAACGCA 11460 0.0 27.436958 5 TACATGG 19115 0.0 26.899082 2 GTACATG 19890 0.0 26.74933 1 AACGCAG 11935 0.0 26.423756 6 ACGCAGA 12550 0.0 24.604588 7 ACATGGG 20295 0.0 24.385618 3 CGCAGAG 12620 0.0 24.207417 8 CATGGGG 10770 0.0 22.823404 4 GCAGAGT 13850 0.0 21.107414 9 CCGGTCG 840 0.0 20.142698 9 CATGGGT 2900 0.0 19.12399 4 ATACGAC 125 0.0064138994 18.799852 3 CATGGGA 6685 0.0 18.56081 4 CCCGGTC 965 0.0 18.507626 8 TACGACT 155 0.0010339264 18.193405 4 >>END_MODULE