##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431351_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3186145
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70485743743615	34.0	31.0	34.0	31.0	34.0
2	32.82792434117091	34.0	31.0	34.0	31.0	34.0
3	32.903258326284586	34.0	31.0	34.0	31.0	34.0
4	36.30466410034697	37.0	37.0	37.0	35.0	37.0
5	36.26298614783696	37.0	37.0	37.0	35.0	37.0
6	36.29976350731056	37.0	37.0	37.0	35.0	37.0
7	36.16895590125371	37.0	37.0	37.0	35.0	37.0
8	36.22650224644516	37.0	37.0	37.0	35.0	37.0
9	38.033913396910684	39.0	39.0	39.0	35.0	39.0
10-11	38.02365601691072	39.0	39.0	39.0	35.0	39.0
12-13	37.9924628351817	39.0	39.0	39.0	35.0	39.0
14-15	39.42867289467366	41.0	39.0	41.0	36.0	41.0
16-17	39.44811614035143	41.0	39.0	41.0	36.0	41.0
18-19	39.53261182400675	41.0	39.5	41.0	36.5	41.0
20-21	39.532077949999135	41.0	40.0	41.0	36.5	41.0
22-23	39.45027203721111	41.0	39.5	41.0	36.0	41.0
24-25	39.402607696762075	41.0	39.0	41.0	36.0	41.0
26-27	39.29307878329455	41.0	39.0	41.0	35.5	41.0
28-29	39.19252466538717	41.0	39.0	41.0	35.0	41.0
30-31	38.98226351908026	41.0	38.5	41.0	35.0	41.0
32-33	38.735533379679836	41.0	38.0	41.0	35.0	41.0
34-35	38.61425500094943	41.0	38.0	41.0	35.0	41.0
36-37	38.48710024182829	41.0	38.0	41.0	35.0	41.0
38-39	38.35117563701589	40.0	38.0	41.0	35.0	41.0
40-41	38.22372851831916	40.0	38.0	41.0	35.0	41.0
42-43	38.042583906256624	40.0	38.0	41.0	34.0	41.0
44-45	37.77026861614898	40.0	38.0	41.0	33.5	41.0
46-47	37.365231180627376	40.0	37.0	41.0	33.0	41.0
48-49	37.00440249894465	40.0	37.0	41.0	32.0	41.0
50-51	36.78869511588455	40.0	37.0	41.0	31.0	41.0
52-53	36.6333617898746	40.0	36.0	41.0	31.0	41.0
54-55	36.44854942257807	40.0	35.5	41.0	31.0	41.0
56-57	36.238952244797396	39.5	35.0	41.0	31.0	41.0
58-59	36.01211416931747	39.0	35.0	41.0	30.0	41.0
60-61	35.77766988005882	39.0	35.0	41.0	30.0	41.0
62-63	35.51640038353559	38.5	35.0	41.0	30.0	41.0
64-65	35.22193669779624	37.5	35.0	40.5	29.0	41.0
66-67	34.9138794687624	37.0	35.0	40.0	29.0	41.0
68-69	34.63379067807648	36.5	35.0	39.5	29.0	41.0
70-71	34.37498371856899	36.0	35.0	39.0	29.0	41.0
72-73	34.121281360390064	36.0	35.0	39.0	29.0	41.0
74-75	33.83386725965077	35.0	35.0	38.5	29.0	40.5
76-77	33.54656881592018	35.0	35.0	37.0	29.0	39.5
78-79	33.262555219552155	35.0	34.0	37.0	29.0	39.0
80-81	32.99634715306428	35.0	34.0	36.5	28.0	39.0
82-83	32.72709041804438	35.0	34.0	36.0	27.5	38.0
84-85	32.508661250508055	35.0	34.0	36.0	27.0	37.0
86-87	32.327778082918385	35.0	34.0	35.0	27.0	37.0
88-89	32.1430410417605	35.0	34.0	35.0	27.0	36.0
90-91	31.978862543920627	35.0	34.0	35.0	26.0	36.0
92-93	31.8591095194977	35.0	34.0	35.0	26.0	36.0
94-95	31.735575593703363	35.0	34.0	35.0	25.5	36.0
96-97	31.612426458933918	35.0	34.0	35.0	25.0	36.0
98-99	31.49995668747028	35.0	34.0	35.0	25.0	35.0
100	31.400298479824365	35.0	34.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2452815442074865
1101	2	0.19145635778763648
1101	3	0.20162461844367385
1101	4	0.10626612899723398
1101	5	0.1581162341465756
1101	6	0.17513362689062717
1101	7	0.18771289155895232
1101	8	0.19135271807986953
1101	9	0.2362772887099851
1101	10-11	0.20743830997258783
1101	12-13	0.21037046706111795
1101	14-15	0.2879301433168493
1101	16-17	0.3272767660445908
1101	18-19	0.2907736891060537
1101	20-21	0.29751277388032094
1101	22-23	0.31392381130692826
1101	24-25	0.2956841236780434
1101	26-27	0.24049696469644744
1101	28-29	0.26185554894365737
1101	30-31	0.29569960194045564
1101	32-33	0.3181542948237279
1101	34-35	0.3289622158991321
1101	36-37	0.338083943079468
1101	38-39	0.346416670164011
1101	40-41	0.2582722694206723
1101	42-43	0.335928958386539
1101	44-45	0.352297163488295
1101	46-47	0.4176820714381222
1101	48-49	0.3696065260249526
1101	50-51	0.3949600131835993
1101	52-53	0.4181447481660783
1101	54-55	0.4235328195658852
1101	56-57	0.3588066879927254
1101	58-59	0.378628171718546
1101	60-61	0.425561732607747
1101	62-63	0.38334727773087707
1101	64-65	0.42153837811719796
1101	66-67	0.42530930968675307
1101	68-69	0.45318139250512957
1101	70-71	0.4406259623902997
1101	72-73	0.3806277308029351
1101	74-75	0.3894806485596689
1101	76-77	0.3922559464840347
1101	78-79	0.3402449812805628
1101	80-81	0.329030546154236
1101	82-83	0.4395988483862894
1101	84-85	0.3634240639269848
1101	86-87	0.3417804591778335
1101	88-89	0.3819647995477524
1101	90-91	0.3599650590897596
1101	92-93	0.41281529233791403
1101	94-95	0.4292066560081267
1101	96-97	0.46481603196089694
1101	98-99	0.4596976946056941
1101	100	0.4609324925443872
1102	1	0.07538967906938865
1102	2	0.12886843855915942
1102	3	0.1470946613698061
1102	4	0.08093371225898238
1102	5	0.15123069617251872
1102	6	0.12456584939609883
1102	7	0.16833391291811495
1102	8	0.17480507587573868
1102	9	0.21041569537293725
1102	10-11	0.21418667079370834
1102	12-13	0.21166179488763248
1102	14-15	0.2834345082183489
1102	16-17	0.2870836588718362
1102	18-19	0.2929082159460634
1102	20-21	0.2696743696739716
1102	22-23	0.2393266238362486
1102	24-25	0.2516051023889503
1102	26-27	0.24635359851309602
1102	28-29	0.2331734423214158
1102	30-31	0.17825194672840894
1102	32-33	0.21322228032413193
1102	34-35	0.27633209946819903
1102	36-37	0.2400460747348987
1102	38-39	0.2790967180718056
1102	40-41	0.26873991816500364
1102	42-43	0.23543005640784287
1102	44-45	0.25978529426659946
1102	46-47	0.2994867629873639
1102	48-49	0.19353469171119286
1102	50-51	0.04653699166576075
1102	52-53	0.05447150343962193
1102	54-55	0.15603661622676412
1102	56-57	0.11360839174807325
1102	58-59	0.1502419549566909
1102	60-61	0.04054785078326972
1102	62-63	0.07704549524339654
1102	64-65	0.1182520766629267
1102	66-67	0.0913463206363545
1102	68-69	0.15268852128713917
1102	70-71	0.10671814624252107
1102	72-73	0.05836033996171608
1102	74-75	0.0496218106459807
1102	76-77	0.058379240604338634
1102	78-79	0.07851736667899445
1102	80-81	-0.003851134549300639
1102	82-83	0.06974598686584699
1102	84-85	0.10467890716143557
1102	86-87	0.09235161953646553
1102	88-89	0.07786374131536888
1102	90-91	0.12103618662655435
1102	92-93	0.08871415758837031
1102	94-95	0.09991322758409282
1102	96-97	0.09724041185976162
1102	98-99	0.12903435724111034
1102	100	0.15476345726876772
1103	1	0.15107121323772787
1103	2	0.16246704328874984
1103	3	0.13774550950364528
1103	4	0.10238347633277556
1103	5	0.09515091546911236
1103	6	0.12021483707648883
1103	7	0.13032392320884156
1103	8	0.09361673885833
1103	9	0.2594088307078337
1103	10-11	0.24666434983173957
1103	12-13	0.21680302709511778
1103	14-15	0.32057456920618677
1103	16-17	0.3506298653087043
1103	18-19	0.3325718591021314
1103	20-21	0.3242857708193938
1103	22-23	0.30965703997060956
1103	24-25	0.282937169140709
1103	26-27	0.2734182937162686
1103	28-29	0.3060574029829297
1103	30-31	0.36196871364455063
1103	32-33	0.34988537738882997
1103	34-35	0.4250351872236635
1103	36-37	0.3823935837479979
1103	38-39	0.3937937976277439
1103	40-41	0.43265293455034026
1103	42-43	0.4693651802360037
1103	44-45	0.4519316622812184
1103	46-47	0.47157042008339545
1103	48-49	0.43784189328859213
1103	50-51	0.35832460174258784
1103	52-53	0.3479638831457237
1103	54-55	0.49821812779889996
1103	56-57	0.4315911674729449
1103	58-59	0.3862627227535853
1103	60-61	0.34682634364277476
1103	62-63	0.32448249415779173
1103	64-65	0.36303541595887623
1103	66-67	0.41725401586600697
1103	68-69	0.4168088552967646
1103	70-71	0.3909791082353209
1103	72-73	0.3942872837672624
1103	74-75	0.3356390546305619
1103	76-77	0.3155081916291209
1103	78-79	0.3395642445315161
1103	80-81	0.362732105363186
1103	82-83	0.38137442813235367
1103	84-85	0.3460875989132717
1103	86-87	0.35453953071012023
1103	88-89	0.3758104337994368
1103	90-91	0.4046532565308212
1103	92-93	0.4255963879983078
1103	94-95	0.3774391290977235
1103	96-97	0.3620302950941543
1103	98-99	0.38814378535026606
1103	100	0.3952177085293265
1104	1	0.0449382522316526
1104	2	0.06585440856399316
1104	3	0.08553230303737536
1104	4	0.02215462757647657
1104	5	0.05906404180567648
1104	6	0.03753876284870472
1104	7	-0.011580246049447851
1104	8	0.09126176703662026
1104	9	0.10218371841694562
1104	10-11	0.10224234446658897
1104	12-13	0.12475858510307347
1104	14-15	0.15205529421201902
1104	16-17	0.1582537290745165
1104	18-19	0.16929589925731392
1104	20-21	0.14412556340082716
1104	22-23	0.1615973229697545
1104	24-25	0.1397426284382135
1104	26-27	0.12575722707725845
1104	28-29	0.15096861751185742
1104	30-31	0.16853259934516274
1104	32-33	0.15750731519118943
1104	34-35	0.1705158658137762
1104	36-37	0.13067504655190731
1104	38-39	0.1595235745539938
1104	40-41	0.1982054265400066
1104	42-43	0.22573677292978545
1104	44-45	0.18380081078291965
1104	46-47	0.19318776138136684
1104	48-49	0.20570067837350337
1104	50-51	0.16973404298881434
1104	52-53	0.18162280060211344
1104	54-55	0.22014371954876566
1104	56-57	0.17350017089952274
1104	58-59	0.14489416874865668
1104	60-61	0.11138207731785599
1104	62-63	0.14381609539263707
1104	64-65	0.1588761871223454
1104	66-67	0.1622161287942987
1104	68-69	0.1527945340250696
1104	70-71	0.17189635892633248
1104	72-73	0.16111055052292755
1104	74-75	0.17310117213301623
1104	76-77	0.11821581699031469
1104	78-79	0.06828904101254807
1104	80-81	0.10985396964188254
1104	82-83	0.14281248438069838
1104	84-85	0.15972309280203945
1104	86-87	0.14282363978782797
1104	88-89	0.19367943612051164
1104	90-91	0.2108904747539455
1104	92-93	0.2962519561079411
1104	94-95	0.24313555796664588
1104	96-97	0.25735259207906935
1104	98-99	0.2365522124804258
1104	100	0.23144610198786708
1105	1	0.02973446181661643
1105	2	0.07936683122865418
1105	3	0.08848068920164565
1105	4	-0.02790744396837397
1105	5	-0.04280160317988191
1105	6	-0.08272371920976695
1105	7	-0.4586583293458162
1105	8	-0.4030941347735535
1105	9	-0.3798018028809338
1105	10-11	-0.37940325657487506
1105	12-13	-0.24637037233049597
1105	14-15	-0.23506779938335143
1105	16-17	-0.16145936386314474
1105	18-19	-0.21872611520301888
1105	20-21	-0.22500912027079067
1105	22-23	-0.16272561930454543
1105	24-25	-0.14115187905830595
1105	26-27	-0.1415897003299733
1105	28-29	-0.20144310363636464
1105	30-31	-0.22123663406269856
1105	32-33	-0.16593295339491476
1105	34-35	-0.2249986380531439
1105	36-37	-0.16567069708356286
1105	38-39	-0.18429226621370987
1105	40-41	-0.16231397786634005
1105	42-43	-0.1258164131928936
1105	44-45	-0.1971843437441052
1105	46-47	-0.249634832055051
1105	48-49	-0.2064689652157199
1105	50-51	-0.18419873151982102
1105	52-53	-0.1668810958373541
1105	54-55	-0.17290065151544098
1105	56-57	-0.20133143261033126
1105	58-59	-0.18605884324336586
1105	60-61	-0.2106812857920346
1105	62-63	-0.16499616806434858
1105	64-65	-0.035813976528991986
1105	66-67	-0.06354943573230543
1105	68-69	-0.13501096054045547
1105	70-71	-0.13523110894652746
1105	72-73	-0.02663596068293117
1105	74-75	-0.12532198683219775
1105	76-77	-0.12969787448844983
1105	78-79	-0.15100013009998747
1105	80-81	-0.1551686586441079
1105	82-83	-0.12316872733521222
1105	84-85	-0.09843790074140202
1105	86-87	-0.11289365620895353
1105	88-89	-0.11422068884348846
1105	90-91	-0.13684560209964758
1105	92-93	-0.1151467894829672
1105	94-95	-0.1126241321672019
1105	96-97	-0.1121841908939274
1105	98-99	-0.1083638675532157
1105	100	-0.11037329058369849
1106	1	0.125615767149462
1106	2	0.096418034663877
1106	3	0.06422294986388977
1106	4	0.06539318508367842
1106	5	0.0683365727567562
1106	6	0.11711438842085897
1106	7	0.08689647290626823
1106	8	0.09587060158565208
1106	9	0.0902343240435215
1106	10-11	0.10626944587086484
1106	12-13	0.10989062792761928
1106	14-15	0.08846788290937013
1106	16-17	0.12264173720390659
1106	18-19	0.14438054231863617
1106	20-21	0.1626407365654643
1106	22-23	0.19405238825930837
1106	24-25	0.16661451266899974
1106	26-27	0.11869216153402107
1106	28-29	0.14620717923244797
1106	30-31	0.20799679303261343
1106	32-33	0.13309943039300975
1106	34-35	0.1130712264707725
1106	36-37	0.1539026553888263
1106	38-39	0.09322379323772623
1106	40-41	0.11183994442414047
1106	42-43	0.11749679472003294
1106	44-45	0.09779160154059952
1106	46-47	0.09802939452304571
1106	48-49	0.05888774825108811
1106	50-51	0.0700796594960309
1106	52-53	-0.0614182366784064
1106	54-55	-0.05411362432471378
1106	56-57	-0.08682941629092511
1106	58-59	-0.062107261434015015
1106	60-61	0.023168299373523382
1106	62-63	0.04403313766110273
1106	64-65	0.012626446838361005
1106	66-67	0.009681721854001069
1106	68-69	0.015500003734260304
1106	70-71	-0.0128851429910668
1106	72-73	-0.016562062203028916
1106	74-75	-0.026441262456955883
1106	76-77	-0.034830758265087525
1106	78-79	0.004350118845188433
1106	80-81	-0.0031601309076876305
1106	82-83	0.026622387269952696
1106	84-85	-0.016065915006862497
1106	86-87	-0.04549346716495961
1106	88-89	-0.0036942874745875542
1106	90-91	0.0033168134994667753
1106	92-93	0.012759650680330026
1106	94-95	0.02916654078119052
1106	96-97	0.015814155118722084
1106	98-99	-0.005857869600806254
1106	100	0.07273945130048176
1107	1	-0.013754991207356682
1107	2	0.05209449480408068
1107	3	0.02570885507833509
1107	4	-0.052678876070586966
1107	5	-0.09265847601854205
1107	6	-0.08826800872078167
1107	7	-0.042023074797363336
1107	8	0.014218883214077493
1107	9	0.04375253625694597
1107	10-11	0.017932937275823235
1107	12-13	0.04623207945791563
1107	14-15	0.043991493775337176
1107	16-17	0.06268952079845036
1107	18-19	0.07521707467001448
1107	20-21	0.08591507129202824
1107	22-23	0.0839510326116013
1107	24-25	0.046040777956704915
1107	26-27	0.05639686280163403
1107	28-29	0.07722551326027371
1107	30-31	0.12123354018169863
1107	32-33	0.09004330620176404
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2216	54-55	-0.7064125487240958
2216	56-57	-0.7211992448270266
2216	58-59	-0.8027002271004946
2216	60-61	-0.8338265963530063
2216	62-63	-0.8822657083878696
2216	64-65	-0.8006391462763389
2216	66-67	-0.8027657163077322
2216	68-69	-0.7832567122936354
2216	70-71	-0.7295981255823776
2216	72-73	-0.7802411808120624
2216	74-75	-0.7445952112109637
2216	76-77	-0.6944361419031679
2216	78-79	-0.5951916235400958
2216	80-81	-0.5980290415943301
2216	82-83	-0.6711798660990809
2216	84-85	-0.6518055804534697
2216	86-87	-0.6333188253874127
2216	88-89	-0.6105645251502594
2216	90-91	-0.6267716160049659
2216	92-93	-0.7814951461174644
2216	94-95	-0.66904277630276
2216	96-97	-0.8433464302837201
2216	98-99	-0.8378390433608587
2216	100	-0.8516221383781577
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46.0
3	327.0
4	223.0
5	384.0
6	472.0
7	850.0
8	1643.0
9	2787.0
10	3251.0
11	4137.0
12	6356.0
13	9400.0
14	9946.0
15	13152.0
16	18017.0
17	28826.0
18	36093.0
19	18512.0
20	10220.0
21	8781.0
22	8745.0
23	8616.0
24	9648.0
25	11269.0
26	13887.0
27	17829.0
28	21858.0
29	27747.0
30	34953.0
31	44043.0
32	55090.0
33	71019.0
34	99179.0
35	172492.0
36	308566.0
37	686867.0
38	989267.0
39	427179.0
40	4468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.35463557429669	20.7765747543365	14.933948802553282	23.934840868813527
2	13.900878303052217	21.822208914600218	39.91065689435255	24.36625588799502
3	15.47436485722195	29.104080368245654	30.808873700855017	24.61268107367738
4	10.478133766722095	18.684837105711328	39.21198447986129	31.62504464770528
5	11.891298104762967	38.88828035133366	34.571464889388274	14.648956654515096
6	27.854538949106207	39.621015364962986	18.332373448163846	14.192072237766956
7	25.200767698896314	34.08300626619315	23.198316460801376	17.51790957410915
8	25.79838645133853	34.13940671250053	20.25711321989426	19.80509361626668
9	26.883647793807246	15.288695272814012	21.29608037298993	36.53157656038881
10-11	24.03289869105141	26.858601852709153	28.256623599993098	20.85187585624634
12-13	25.48498263575575	25.558551164495025	28.33764627786871	20.618819921880515
14-15	22.76020080693126	25.961498927387172	27.677020349042497	23.601279916639072
16-17	20.5835735661748	28.934433304196766	27.90216703885103	22.579826090777413
18-19	20.510993693005183	26.601127695067234	32.2552488979629	20.632629713964683
20-21	22.246476541400344	25.635258282344335	31.84946071192617	20.26880446432915
22-23	21.64685717818873	25.70811748316891	32.05271956691189	20.592305771730476
24-25	20.962612614662593	25.952080282686758	32.07785969329988	21.007447409350778
26-27	20.85201916873645	26.24934265772017	31.979044767696692	20.919593405846694
28-29	20.849988952051905	25.875141865697127	32.583113839061525	20.691755343189442
30-31	21.232782015724894	26.1192660795215	31.94966439319361	20.698287511559997
32-33	20.84450390574425	26.063974953017286	32.095385689792494	20.99613545144597
34-35	21.260782785584723	25.696418151897326	32.025818735265524	21.016980327252423
36-37	21.38080903098516	25.9969761541791	31.441562155034713	21.180652659801027
38-39	21.16422276943145	26.105819230646706	31.602300705996157	21.12765729392569
40-41	21.17426555437174	25.989622493605275	31.468737459885364	21.367374492137618
42-43	21.098726164627514	26.277774325309167	31.45454699531657	21.16895251474675
44-45	21.320702130577807	26.17001623599281	30.98651624807643	21.522765385352958
46-47	21.63841494926053	26.174325700899598	30.391014775282283	21.796244574557587
48-49	21.66829694079178	26.398294199169225	29.811684898901298	22.121723961137697
50-51	21.87829015974179	26.56106873882748	29.25997073861688	22.300670362813847
52-53	21.851092778263386	26.927399726001173	29.06426104273346	22.15724645300198
54-55	21.914597386423413	27.206264523880368	29.03043565050036	21.848702439195865
56-57	21.897991777160065	26.820381055297414	29.050236721685945	22.231390445856576
58-59	22.05230652619289	26.059434312396967	29.46366625890875	22.424592902501395
60-61	22.123921302769656	26.50785355924969	29.28336369927828	22.084861438702376
62-63	21.686301157040873	26.48388569886179	29.57065670269244	22.259156441404894
64-65	21.889399258351393	26.56348031869234	29.439134126036322	22.107986296919947
66-67	21.86583229680517	26.48923438781438	29.383698087672233	22.26123522770821
68-69	22.22166250250893	26.58981453451904	29.18535515863581	22.003167804336215
70-71	22.192363065583326	26.39588157067534	29.250072738537682	22.16168262520366
72-73	21.98395615515181	26.385310896180464	29.165678010786706	22.465054937881025
74-75	22.04656459067939	26.63888549305175	28.996994906453537	22.317555009815322
76-77	22.191092522218163	26.54729935859531	28.865549198840473	22.39605892034605
78-79	22.238870897578423	26.53165023385914	28.929906914584762	22.299571953977676
80-81	21.824139198927597	26.825619841584576	29.042848872600864	22.307392086886967
82-83	21.88668354830853	26.81152278770561	29.11127508706558	22.190518576920276
84-85	21.825001196601182	26.487132025520165	28.940643088784668	22.747223689093982
86-87	22.005315527863363	26.648336214042274	28.846311379579724	22.50003687851464
88-89	21.643755387669763	26.70407296456524	28.939003535519742	22.713168112245256
90-91	21.610957012323105	26.579056601552846	28.866329000694424	22.94365738542962
92-93	21.57015186680303	26.91216094168058	28.936863327723984	22.580823863792407
94-95	21.678807397240583	26.381960806714904	29.076314762812423	22.86291703323209
96-97	21.78956744775532	26.724885799603648	28.747584011128456	22.737962741512575
98-99	21.30783147126803	26.65613226083059	28.96123058264921	23.074805685252166
100	21.38021082630919	26.555734635683535	29.0526940225076	23.011360515499675
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4169.0
1	2573.5
2	2990.0
3	4203.0
4	2111.0
5	1207.0
6	3087.5
7	8673.0
8	14747.0
9	14638.0
10	8500.0
11	3739.5
12	3036.5
13	3196.5
14	3479.5
15	3799.5
16	4245.5
17	4763.5
18	5246.5
19	5838.5
20	6779.5
21	8036.0
22	9391.5
23	11397.5
24	14469.0
25	17663.0
26	21344.0
27	26508.5
28	32470.5
29	38722.0
30	43477.0
31	48030.0
32	53618.5
33	58173.5
34	62937.5
35	69806.5
36	75483.5
37	81347.0
38	88816.5
39	92450.5
40	97083.5
41	104367.5
42	109183.0
43	117317.0
44	121577.5
45	118246.5
46	116632.0
47	117834.0
48	116421.5
49	114966.0
50	118451.0
51	113480.5
52	101643.5
53	93381.5
54	87716.0
55	83261.0
56	74075.0
57	63014.0
58	58023.0
59	55919.0
60	49434.5
61	39761.0
62	33174.0
63	27560.5
64	21585.5
65	17160.5
66	13549.5
67	10138.5
68	7565.0
69	5639.0
70	4100.0
71	3021.0
72	2238.0
73	1641.0
74	1286.5
75	924.5
76	585.0
77	429.5
78	304.0
79	175.0
80	84.5
81	30.5
82	17.0
83	13.5
84	6.5
85	5.0
86	1.5
87	1.0
88	1.0
89	1.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.5
95	6.0
96	7.5
97	7.0
98	4.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008882207181405742
2	3.138589110037365E-5
3	3.452448021041101E-4
4	0.0028561160901340024
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.0985061885130776E-4
24-25	1.0985061885130776E-4
26-27	6.120248764572862E-4
28-29	0.0010357344063123305
30-31	0.0015849875005688695
32-33	0.004158630570799508
34-35	0.005147286140461279
36-37	0.004268481189650816
38-39	0.0024951783424797054
40-41	0.003750613986494651
42-43	5.963319309070993E-4
44-45	4.3940247540523106E-4
46-47	0.0035936845309927825
48-49	0.00236963477807821
50-51	0.0010828132429628909
52-53	0.0
54-55	4.080165843048574E-4
56-57	6.120248764572862E-4
58-59	7.061825497584071E-4
60-61	3.1385891100373645E-4
62-63	0.0
64-65	0.0
66-67	8.788049508104621E-4
68-69	8.317261141599017E-4
70-71	0.0023853277236283972
72-73	3.138589110037365E-5
74-75	0.001710531064970364
76-77	4.080165843048574E-4
78-79	0.0012240497529145723
80-81	8.94497896360649E-4
82-83	0.0012868215351153197
84-85	0.001176970916264012
86-87	2.510871288029892E-4
88-89	0.0015849875005688695
90-91	0.0018046887382714848
92-93	0.003028738491186057
94-95	0.0011926638618141986
96-97	0.0024794853969295184
98-99	0.002416713614728771
100	0.003452448021041102
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3186145.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.938158522655065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.90737428212897	31.155394516334177
2	15.560253156334888	13.67377739671372
3	5.489478546818682	7.235927357905002
4	2.655351593973336	4.666850370775409
5	1.4210469290605388	3.121909261859706
6	0.8912793199760375	2.349670322944281
7	0.5996379276441521	1.8442890424717588
8	0.40746321904285304	1.4322546808364962
9	0.30216655664766523	1.194897785960695
>10	1.4952340695220823	12.064407018738804
>50	0.14256969879308898	4.412947623115532
>100	0.11527271205210772	9.864626776827912
>500	0.009868524137880618	2.8629540980042756
>1k	0.0026459086455140847	1.7750145735391738
>5k	1.4302208894670726E-4	0.34283056028970343
>10k+	2.145331334200609E-4	2.0022486136835362
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28014	0.8792443532858675	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19504	0.6121504200216876	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16206	0.5086397511726554	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5680	0.17827186145012233	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5231	0.16417959634605456	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4491	0.14095403693177805	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3299	0.10354205474013267	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	3.138589110037365E-5	0.0	0.0	1.5692945550186825E-5	0.0
16-17	3.138589110037365E-5	0.0	0.0	3.138589110037365E-5	0.0
18-19	3.138589110037365E-5	0.0	0.0	6.27717822007473E-5	0.0
20-21	3.138589110037365E-5	0.0	0.0	1.7262240105205508E-4	0.0
22-23	3.138589110037365E-5	0.0	0.0	4.2370952985504426E-4	0.0
24-25	3.138589110037365E-5	0.0	0.0	8.788049508104622E-4	1.5692945550186825E-5
26-27	3.138589110037365E-5	0.0	0.0	0.0015222157183681219	3.138589110037365E-5
28-29	3.138589110037365E-5	0.0	0.0	0.003405369184390541	3.138589110037365E-5
30-31	3.138589110037365E-5	0.0	0.0	0.00825448935939827	3.138589110037365E-5
32-33	3.138589110037365E-5	0.0	0.0	0.01610096213449168	3.138589110037365E-5
34-35	3.138589110037365E-5	0.0	0.0	0.025861974266707888	3.138589110037365E-5
36-37	3.138589110037365E-5	0.0	0.0	0.04208847996560106	3.138589110037365E-5
38-39	3.138589110037365E-5	0.0	0.0	0.06435676970131617	3.138589110037365E-5
40-41	3.138589110037365E-5	0.0	0.0	0.09106616302773415	3.138589110037365E-5
42-43	3.138589110037365E-5	0.0	0.0	0.11504498382841961	3.138589110037365E-5
44-45	3.138589110037365E-5	0.0	0.0	0.14348060116535813	3.138589110037365E-5
46-47	3.138589110037365E-5	0.0	0.0	0.17904081578208147	3.138589110037365E-5
48-49	3.138589110037365E-5	0.0	0.0	0.21681373572138116	3.138589110037365E-5
50-51	3.138589110037365E-5	0.0	0.0	0.25772524477071823	3.138589110037365E-5
52-53	3.138589110037365E-5	0.0	0.0	0.29750686174044183	3.138589110037365E-5
54-55	4.7078836650560476E-5	0.0	0.0	0.3391088603939871	3.138589110037365E-5
56-57	6.27717822007473E-5	0.0	0.0	0.39097404543735453	3.138589110037365E-5
58-59	9.415767330112095E-5	0.0	0.0	0.4517685164987783	3.138589110037365E-5
60-61	9.415767330112095E-5	0.0	0.0	0.5193894188745334	3.138589110037365E-5
62-63	9.415767330112095E-5	0.0	0.0	0.5883756075131545	3.138589110037365E-5
64-65	9.415767330112095E-5	0.0	0.0	0.6616145843958765	3.138589110037365E-5
66-67	9.415767330112095E-5	0.0	0.0	0.7452893700694727	3.138589110037365E-5
68-69	9.415767330112095E-5	0.0	0.0	0.84586545810062	3.138589110037365E-5
70-71	9.415767330112095E-5	0.0	0.0	0.9616480103698984	3.138589110037365E-5
72-73	9.415767330112095E-5	0.0	0.0	1.0851357989043184	3.138589110037365E-5
74-75	9.415767330112095E-5	0.0	0.0	1.2108833715979657	3.138589110037365E-5
76-77	9.415767330112095E-5	0.0	0.0	1.3403344794414567	3.138589110037365E-5
78-79	9.415767330112095E-5	0.0	0.0	1.493560399793481	3.138589110037365E-5
80-81	9.415767330112095E-5	0.0	0.0	1.6758653482500012	3.138589110037365E-5
82-83	9.415767330112095E-5	0.0	0.0	1.8694064457204553	3.138589110037365E-5
84-85	9.415767330112095E-5	0.0	0.0	2.064736538983631	3.138589110037365E-5
86-87	9.415767330112095E-5	0.0	0.0	2.272008963810498	3.138589110037365E-5
88	9.415767330112095E-5	0.0	0.0	2.4400333318163487	3.138589110037365E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8920	0.0	63.075527	1
GTATCAA	18160	0.0	46.14951	1
ATCAACG	24515	0.0	33.973705	3
TCAACGC	24830	0.0	33.542706	4
TATCAAC	25210	0.0	33.14897	2
CAACGCA	25730	0.0	32.367397	5
AACGCAG	26590	0.0	31.355892	6
ACGCAGA	29460	0.0	28.125706	7
CGCAGAG	29805	0.0	27.721302	8
GTGGTAT	3055	0.0	27.694458	1
TGGTATC	2990	0.0	27.352005	2
GCAGAGT	31995	0.0	25.471285	9
GTACATG	18785	0.0	24.346315	1
TACATGG	18270	0.0	23.976648	2
ACATGGG	19055	0.0	22.347574	3
AGAGTAC	31185	0.0	21.611616	10-11
CAGAGTA	31400	0.0	21.493572	10-11
AGTACTT	21975	0.0	21.237541	12-13
GAGTACT	22005	0.0	21.144514	12-13
CATGGGG	10295	0.0	20.909819	4
>>END_MODULE