FastQCFastQC Report
Wed 25 May 2016
SRR2431350_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431350_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2847400
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA567861.994310599143078No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43540.15291142796937557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50350.039.2985531
GTATCAA83650.034.779131
ATCAACG96600.028.608443
TCAACGC97200.028.3834924
TACCTGG22450.028.2625522
CAACGCA103150.026.7462465
GTACCTG24850.026.4786381
AACGCAG107400.025.687856
TACATGG158750.025.1947362
GTACACG3000.025.0664421
GTACATG170200.024.9633461
ACGCAGA116900.023.6807127
CGCAGAG117550.023.5097858
ACCTGGG26400.023.499793
TATAACG1802.163415E-723.4997882
TACACGG2402.6193447E-1023.4997882
TATCAAC121850.023.3744322
ACATGGG167000.022.7399163
GCAGAGT127050.020.8270479
CATGGGT33300.020.7475624
Produced by FastQC (version 0.11.5)